iwuala beatrice - Academia.edu (original) (raw)

Papers by iwuala beatrice

Tropical Journal of Natural Product Research

medicinal chemists as amide derivatives are associated with broad spectrum of biological activiti... more medicinal chemists as amide derivatives are associated with broad spectrum of biological activities including anti-tuberculosis, 7 anticonvulsant, 8 analgesic-anti-inflammatory, 9 insecticidal, 10 antifungal, 11 and antitumor properties. 12 There is a need for discovery of new compounds which can be used clinically and has antibacterial and antifungal activities in which their mechanism of action are distinct from existing antimicrobial agents and do not show resistance compared to the existing antimicrobial agents. 13 Materials and Methods General experimental procedures All chemicals were of analytical grade and purchased from Sigma-Aldrich (Germany) through Bristol Scientific Company and commercial suppliers. Analytical thin layer chromatography (TLC) was performed using aluminum plates coated with silica gel 60F254 (Merck). Spots were visualized using UV lamp or by saturation with Iodine vapour. NMR spectra were recorded using 400 MHz spectrometer at room temperature in DMSO-d6. The infrared spectra of the solid samples were recorded on Agilent FTIR 400s Fourier Transform Infra-Red Spectrophotometer. Melting point determination was carried out on Stuart Automatic Melting Point /SMP40/ apparatus. Synthesis of N,N'-(4-Nitro-1,2-phenylene)diacetamide (3a) An intimate mixture of Acetic anhydride (0.04 mol), 4-Nitro-1,2phenylenediamine (0.02 mol) and 20 mL of toluene was stirred at room temperature for 20 minutes. The reaction was monitor with TLC until completion, the solvent was evaporated using rotary evaporator and the separated solid was filtered, washed with toluene and dried. The crude products were recrystallized from ethanol for further purification (Scheme 1).

medicinal chemists as amide derivatives are associated with broad spectrum of biological activiti... more medicinal chemists as amide derivatives are associated with broad spectrum of biological activities including anti-tuberculosis, 7 anticonvulsant, 8 analgesic-anti-inflammatory, 9 insecticidal, 10 antifungal, 11 and antitumor properties. 12 There is a need for discovery of new compounds which can be used clinically and has antibacterial and antifungal activities in which their mechanism of action are distinct from existing antimicrobial agents and do not show resistance compared to the existing antimicrobial agents. 13 Materials and Methods General experimental procedures All chemicals were of analytical grade and purchased from Sigma-Aldrich (Germany) through Bristol Scientific Company and commercial suppliers. Analytical thin layer chromatography (TLC) was performed using aluminum plates coated with silica gel 60F254 (Merck). Spots were visualized using UV lamp or by saturation with Iodine vapour. NMR spectra were recorded using 400 MHz spectrometer at room temperature in DMSO-d6. The infrared spectra of the solid samples were recorded on Agilent FTIR 400s Fourier Transform Infra-Red Spectrophotometer. Melting point determination was carried out on Stuart Automatic Melting Point /SMP40/ apparatus. Synthesis of N,N'-(4-Nitro-1,2-phenylene)diacetamide (3a) An intimate mixture of Acetic anhydride (0.04 mol), 4-Nitro-1,2phenylenediamine (0.02 mol) and 20 mL of toluene was stirred at room temperature for 20 minutes. The reaction was monitor with TLC until completion, the solvent was evaporated using rotary evaporator and the separated solid was filtered, washed with toluene and dried. The crude products were recrystallized from ethanol for further purification (Scheme 1).

medicinal chemists as amide derivatives are associated with broad spectrum of biological activiti... more medicinal chemists as amide derivatives are associated with broad spectrum of biological activities including anti-tuberculosis, 7 anticonvulsant, 8 analgesic-anti-inflammatory, 9 insecticidal, 10 antifungal, 11 and antitumor properties. 12 There is a need for discovery of new compounds which can be used clinically and has antibacterial and antifungal activities in which their mechanism of action are distinct from existing antimicrobial agents and do not show resistance compared to the existing antimicrobial agents. 13 Materials and Methods General experimental procedures All chemicals were of analytical grade and purchased from Sigma-Aldrich (Germany) through Bristol Scientific Company and commercial suppliers. Analytical thin layer chromatography (TLC) was performed using aluminum plates coated with silica gel 60F254 (Merck). Spots were visualized using UV lamp or by saturation with Iodine vapour. NMR spectra were recorded using 400 MHz spectrometer at room temperature in DMSO-d6. The infrared spectra of the solid samples were recorded on Agilent FTIR 400s Fourier Transform Infra-Red Spectrophotometer. Melting point determination was carried out on Stuart Automatic Melting Point /SMP40/ apparatus. Synthesis of N,N'-(4-Nitro-1,2-phenylene)diacetamide (3a) An intimate mixture of Acetic anhydride (0.04 mol), 4-Nitro-1,2phenylenediamine (0.02 mol) and 20 mL of toluene was stirred at room temperature for 20 minutes. The reaction was monitor with TLC until completion, the solvent was evaporated using rotary evaporator and the separated solid was filtered, washed with toluene and dried. The crude products were recrystallized from ethanol for further purification (Scheme 1).

Tropical Journal of Natural Product Research

medicinal chemists as amide derivatives are associated with broad spectrum of biological activiti... more medicinal chemists as amide derivatives are associated with broad spectrum of biological activities including anti-tuberculosis, 7 anticonvulsant, 8 analgesic-anti-inflammatory, 9 insecticidal, 10 antifungal, 11 and antitumor properties. 12 There is a need for discovery of new compounds which can be used clinically and has antibacterial and antifungal activities in which their mechanism of action are distinct from existing antimicrobial agents and do not show resistance compared to the existing antimicrobial agents. 13 Materials and Methods General experimental procedures All chemicals were of analytical grade and purchased from Sigma-Aldrich (Germany) through Bristol Scientific Company and commercial suppliers. Analytical thin layer chromatography (TLC) was performed using aluminum plates coated with silica gel 60F254 (Merck). Spots were visualized using UV lamp or by saturation with Iodine vapour. NMR spectra were recorded using 400 MHz spectrometer at room temperature in DMSO-d6. The infrared spectra of the solid samples were recorded on Agilent FTIR 400s Fourier Transform Infra-Red Spectrophotometer. Melting point determination was carried out on Stuart Automatic Melting Point /SMP40/ apparatus. Synthesis of N,N'-(4-Nitro-1,2-phenylene)diacetamide (3a) An intimate mixture of Acetic anhydride (0.04 mol), 4-Nitro-1,2phenylenediamine (0.02 mol) and 20 mL of toluene was stirred at room temperature for 20 minutes. The reaction was monitor with TLC until completion, the solvent was evaporated using rotary evaporator and the separated solid was filtered, washed with toluene and dried. The crude products were recrystallized from ethanol for further purification (Scheme 1).

medicinal chemists as amide derivatives are associated with broad spectrum of biological activiti... more medicinal chemists as amide derivatives are associated with broad spectrum of biological activities including anti-tuberculosis, 7 anticonvulsant, 8 analgesic-anti-inflammatory, 9 insecticidal, 10 antifungal, 11 and antitumor properties. 12 There is a need for discovery of new compounds which can be used clinically and has antibacterial and antifungal activities in which their mechanism of action are distinct from existing antimicrobial agents and do not show resistance compared to the existing antimicrobial agents. 13 Materials and Methods General experimental procedures All chemicals were of analytical grade and purchased from Sigma-Aldrich (Germany) through Bristol Scientific Company and commercial suppliers. Analytical thin layer chromatography (TLC) was performed using aluminum plates coated with silica gel 60F254 (Merck). Spots were visualized using UV lamp or by saturation with Iodine vapour. NMR spectra were recorded using 400 MHz spectrometer at room temperature in DMSO-d6. The infrared spectra of the solid samples were recorded on Agilent FTIR 400s Fourier Transform Infra-Red Spectrophotometer. Melting point determination was carried out on Stuart Automatic Melting Point /SMP40/ apparatus. Synthesis of N,N'-(4-Nitro-1,2-phenylene)diacetamide (3a) An intimate mixture of Acetic anhydride (0.04 mol), 4-Nitro-1,2phenylenediamine (0.02 mol) and 20 mL of toluene was stirred at room temperature for 20 minutes. The reaction was monitor with TLC until completion, the solvent was evaporated using rotary evaporator and the separated solid was filtered, washed with toluene and dried. The crude products were recrystallized from ethanol for further purification (Scheme 1).

medicinal chemists as amide derivatives are associated with broad spectrum of biological activiti... more medicinal chemists as amide derivatives are associated with broad spectrum of biological activities including anti-tuberculosis, 7 anticonvulsant, 8 analgesic-anti-inflammatory, 9 insecticidal, 10 antifungal, 11 and antitumor properties. 12 There is a need for discovery of new compounds which can be used clinically and has antibacterial and antifungal activities in which their mechanism of action are distinct from existing antimicrobial agents and do not show resistance compared to the existing antimicrobial agents. 13 Materials and Methods General experimental procedures All chemicals were of analytical grade and purchased from Sigma-Aldrich (Germany) through Bristol Scientific Company and commercial suppliers. Analytical thin layer chromatography (TLC) was performed using aluminum plates coated with silica gel 60F254 (Merck). Spots were visualized using UV lamp or by saturation with Iodine vapour. NMR spectra were recorded using 400 MHz spectrometer at room temperature in DMSO-d6. The infrared spectra of the solid samples were recorded on Agilent FTIR 400s Fourier Transform Infra-Red Spectrophotometer. Melting point determination was carried out on Stuart Automatic Melting Point /SMP40/ apparatus. Synthesis of N,N'-(4-Nitro-1,2-phenylene)diacetamide (3a) An intimate mixture of Acetic anhydride (0.04 mol), 4-Nitro-1,2phenylenediamine (0.02 mol) and 20 mL of toluene was stirred at room temperature for 20 minutes. The reaction was monitor with TLC until completion, the solvent was evaporated using rotary evaporator and the separated solid was filtered, washed with toluene and dried. The crude products were recrystallized from ethanol for further purification (Scheme 1).