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El presente documento describe el resultado de un proceso de investigacion y desarrollo tecnologi... more El presente documento describe el resultado de un proceso de investigacion y desarrollo tecnologico para el diseno y la implementacion de un sistema optoelectronico de medicion de pH. Se utiliza como sensor una pelicula delgada obtenida por el metodo dip-coating, en la cual se constituye una matriz porosa de vidrio que se deposita sobre un sustrato sodico calcico donde se inmovilizan las moleculas de los indicadores organicos de pH que actuan como dopantes de la pelicula delgada. Cuando la pelicula se somete a un ambiente acido, cambia de color el cual es captado por un sensor optico y mediante procesamiento de senales realizado por un microcontrolador se tiene una estimacion cuantitativa que es proporcional al pH del ambiente acido. El valor obtenido se muestra numericamente en una pantalla de cristal liquido.
Microbiome, Oct 17, 2017
Shotgun metagenomic sequencing is increasingly utilized as a tool to evaluate ecological-level dy... more Shotgun metagenomic sequencing is increasingly utilized as a tool to evaluate ecological-level dynamics of antimicrobial resistance and virulence, in conjunction with microbiome analysis. Interest in use of this method for environmental surveillance of antimicrobial resistance and pathogenic microorganisms is also increasing. In published metagenomic datasets, the total of all resistance- and virulence-related sequences accounts for < 1% of all sequenced DNA, leading to limitations in detection of low-abundance resistome-virulome elements. This study describes the extent and composition of the low-abundance portion of the resistome-virulome, using a bait-capture and enrichment system that incorporates unique molecular indices to count DNA molecules and correct for enrichment bias. The use of the bait-capture and enrichment system significantly increased on-target sequencing of the resistome-virulome, enabling detection of an additional 1441 gene accessions and revealing a low-abu...
Nucleic acids research, Jan 4, 2017
Antimicrobial resistance has become an imminent concern for public health. As methods for detecti... more Antimicrobial resistance has become an imminent concern for public health. As methods for detection and characterization of antimicrobial resistance move from targeted culture and polymerase chain reaction to high throughput metagenomics, appropriate resources for the analysis of large-scale data are required. Currently, antimicrobial resistance databases are tailored to smaller-scale, functional profiling of genes using highly descriptive annotations. Such characteristics do not facilitate the analysis of large-scale, ecological sequence datasets such as those produced with the use of metagenomics for surveillance. In order to overcome these limitations, we present MEGARes (https://megares.meglab.org), a hand-curated antimicrobial resistance database and annotation structure that provides a foundation for the development of high throughput acyclical classifiers and hierarchical statistical analysis of big data. MEGARes can be browsed as a stand-alone resource through the website or...
Hogar Y Arquitectura Revista Bimestral De La Obra Sindical Del Hogar, 1965
Arquitectura Revista Del Colegio Oficial De Arquitectos De Madrid, 1959
Avian Diseases, 2003
Killed vaccines in oil emulsions are critical components of breeder and layer vaccination program... more Killed vaccines in oil emulsions are critical components of breeder and layer vaccination programs. Current vaccination sites are limited, and each has inherent problems. Oil emulsion vaccines are associated with increased condemnations of spent fowl when vaccines are injected intramuscularly into the breast. In an attempt to reduce tissue reaction when injected into the breast muscle, a commercially available Pasteurella multocida bacterin was heated to 41 C (100 F) for 5 hr prior to administration. A second treatment group was injected with the same bacterin at room temperature, 25 C. The vaccine was injected into the breast muscle at 10 and 18 wk of age into white Leghorn hens. Seroconversion was evaluated using P. multocida enzyme-linked immunosorbent assay (ELISA) at 10, 18, and 24 wk. Treatment and control groups were euthanized and lesions scored at 24 wk of age. One replicate was challenged with type 1 P. multocida at 24 wk of age. Lesion scores for the heated vaccine group were significantly lower than the room temperature vaccine. ELISA titers were not significantly different at 24 wk between the two treatment group; however, a significant rise in antibody titer was observed at 18 wk in the group that was injected with the heated vaccine. Survivability to challenge was improved in birds injected with the heated vaccine. Results suggest that heating of a P. multocida bacterin reduces local tissue reaction without having a deleterious effect on immunity as measured by ELISA and challenge.
El presente documento describe el resultado de un proceso de investigacion y desarrollo tecnologi... more El presente documento describe el resultado de un proceso de investigacion y desarrollo tecnologico para el diseno y la implementacion de un sistema optoelectronico de medicion de pH. Se utiliza como sensor una pelicula delgada obtenida por el metodo dip-coating, en la cual se constituye una matriz porosa de vidrio que se deposita sobre un sustrato sodico calcico donde se inmovilizan las moleculas de los indicadores organicos de pH que actuan como dopantes de la pelicula delgada. Cuando la pelicula se somete a un ambiente acido, cambia de color el cual es captado por un sensor optico y mediante procesamiento de senales realizado por un microcontrolador se tiene una estimacion cuantitativa que es proporcional al pH del ambiente acido. El valor obtenido se muestra numericamente en una pantalla de cristal liquido.
Microbiome, Oct 17, 2017
Shotgun metagenomic sequencing is increasingly utilized as a tool to evaluate ecological-level dy... more Shotgun metagenomic sequencing is increasingly utilized as a tool to evaluate ecological-level dynamics of antimicrobial resistance and virulence, in conjunction with microbiome analysis. Interest in use of this method for environmental surveillance of antimicrobial resistance and pathogenic microorganisms is also increasing. In published metagenomic datasets, the total of all resistance- and virulence-related sequences accounts for < 1% of all sequenced DNA, leading to limitations in detection of low-abundance resistome-virulome elements. This study describes the extent and composition of the low-abundance portion of the resistome-virulome, using a bait-capture and enrichment system that incorporates unique molecular indices to count DNA molecules and correct for enrichment bias. The use of the bait-capture and enrichment system significantly increased on-target sequencing of the resistome-virulome, enabling detection of an additional 1441 gene accessions and revealing a low-abu...
Nucleic acids research, Jan 4, 2017
Antimicrobial resistance has become an imminent concern for public health. As methods for detecti... more Antimicrobial resistance has become an imminent concern for public health. As methods for detection and characterization of antimicrobial resistance move from targeted culture and polymerase chain reaction to high throughput metagenomics, appropriate resources for the analysis of large-scale data are required. Currently, antimicrobial resistance databases are tailored to smaller-scale, functional profiling of genes using highly descriptive annotations. Such characteristics do not facilitate the analysis of large-scale, ecological sequence datasets such as those produced with the use of metagenomics for surveillance. In order to overcome these limitations, we present MEGARes (https://megares.meglab.org), a hand-curated antimicrobial resistance database and annotation structure that provides a foundation for the development of high throughput acyclical classifiers and hierarchical statistical analysis of big data. MEGARes can be browsed as a stand-alone resource through the website or...
Hogar Y Arquitectura Revista Bimestral De La Obra Sindical Del Hogar, 1965
Arquitectura Revista Del Colegio Oficial De Arquitectos De Madrid, 1959
Avian Diseases, 2003
Killed vaccines in oil emulsions are critical components of breeder and layer vaccination program... more Killed vaccines in oil emulsions are critical components of breeder and layer vaccination programs. Current vaccination sites are limited, and each has inherent problems. Oil emulsion vaccines are associated with increased condemnations of spent fowl when vaccines are injected intramuscularly into the breast. In an attempt to reduce tissue reaction when injected into the breast muscle, a commercially available Pasteurella multocida bacterin was heated to 41 C (100 F) for 5 hr prior to administration. A second treatment group was injected with the same bacterin at room temperature, 25 C. The vaccine was injected into the breast muscle at 10 and 18 wk of age into white Leghorn hens. Seroconversion was evaluated using P. multocida enzyme-linked immunosorbent assay (ELISA) at 10, 18, and 24 wk. Treatment and control groups were euthanized and lesions scored at 24 wk of age. One replicate was challenged with type 1 P. multocida at 24 wk of age. Lesion scores for the heated vaccine group were significantly lower than the room temperature vaccine. ELISA titers were not significantly different at 24 wk between the two treatment group; however, a significant rise in antibody titer was observed at 18 wk in the group that was injected with the heated vaccine. Survivability to challenge was improved in birds injected with the heated vaccine. Results suggest that heating of a P. multocida bacterin reduces local tissue reaction without having a deleterious effect on immunity as measured by ELISA and challenge.