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Papers by jimena verónica lavandera

European Journal of Nutrition

Purpose: We examined the effect of a functional milk fat (FMF) on the glucose metabolism and its ... more Purpose: We examined the effect of a functional milk fat (FMF) on the glucose metabolism and its association with the intramuscular triacylglycerol (TAG) content in rats fed high-fat diets. Methods: Male Wistar rats were fed for 60 days with S7 (soybean oil 7%), S30 (soybean oil 30%), MF30 (soybean oil 3% + milk fat 27%), or FMF30 (soybean oil 3% + FMF 27%) diets. An oral glucose tolerance test was performed. The levels of key metabolites in gastrocnemius muscle and mRNA levels of genes involved in glucose and lipid metabolism in muscle, epididymal white adipose tissue (EWAT), and serum were assessed. Results: The S30 diet induced glucose intolerance and led to TAG, citrate, and glucose accumulation in muscle. Moreover, we observed a downregulation of uncoupling proteins (Ucp2 and Ucp3) and insulin receptor substrate-1 (Irs1) genes, lower carnitine palmitoyl transferase-1b (CPT-1b), and phosphofructokinase-1 (PFK1) activities in muscle and lower expression of adiponectin (Adipoq) in EWAT. The FMF30 diet ameliorated the glucose intolerance and normalized the glucose and TAG levels in muscle, preventing the accumulation of citrate and enhancing glucose utilization by the PFK1. The beneficial effects might also be related to the higher expression of Adipoq in EWAT, its receptor in muscle (Adipor1), and the expression of Ucp2, Ucp3, and Irs1 in muscle, restoring the alterations observed with the S30 diet. Conclusions: FMF30 modulated key genes involved in glucose and lipid metabolism in skeletal muscle, improving the glucose utilization and preventing TAG, glucose, and citrate accumulation.

Revista Española de Nutrición Humana y Dietética

Introducción: Las Guías Alimentarias para la Población Argentina recomiendan una ingesta diaria d... more Introducción: Las Guías Alimentarias para la Población Argentina recomiendan una ingesta diaria de 3 porciones de leche, yogur y/o queso, preferentemente descremados. Sin embargo, la Segunda Encuesta Nacional de Nutrición y Salud (2019) informó que solo 4 de 10 adolescentes y adultos los consumen, al menos una vez al día. El objetivo del presente trabajo fue determinar el contenido de minerales y la contribución de una porción de los alimentos antes mencionados a la ingesta diaria recomendada (IDR). Material y métodos: Se cuantificó calcio (Ca), sodio (Na), potasio (K), hierro (Fe), zinc (Zn), manganeso (Mn) y cobre (Cu) por absorción atómica y fósforo (P) por espectrofotometría visible en 12 leches, 11 yogures, 22 quesos blandos (QB), 23 quesos semiduros (QSD) y 12 quesos duros (QD); y se estableció que un alimento es fuente de algún mineral cuando una porción aporta más del 15% de la IDR para dicho nutriente. Resultados: En promedio, el contenido de Ca, P y Na en yogures es signif...

Food & Function, 2021

Potential effect of functional milk fat, naturally enriched in conjugated linoleic acid, on the p... more Potential effect of functional milk fat, naturally enriched in conjugated linoleic acid, on the prevention of liver lipid accumulation and some biochemical mechanisms involved in the liver triacylglycerol regulation in high-fat fed rats.

de gallinas ponedoras podria mejorar el perfil de los acidos grasos (AG) en los huevos. Esta prac... more de gallinas ponedoras podria mejorar el perfil de los acidos grasos (AG) en los huevos. Esta practica ha conducido al empleo de suplementos con diversos tipos de aceites ricos en PUFA n-3, como el aceite de lino, con la finalidad de ofrecer al consumidor huevos con una mejor calidad nutricional. No obstante, este tipo de aceites son muy inestables, dando diversos productos que podrian provocar alteraciones hepaticas que van desde esteatosis a danos irreversibles que comprometerian la salud de las gallinas.

Los lipidos son componentes importantes de las membranas celulares y juegan un papel importante e... more Los lipidos son componentes importantes de las membranas celulares y juegan un papel importante en los procesos metabolicos. Estan compuestos por acidos grasos (FA) de diferentes longitudes de cadena que pueden ser saturadas o insaturadas. Los cambios en la composicion de AG de la dieta podrian inducir alteraciones en la composicion de AG de los tejidos.

Dietas ricas en grasas desarrollan hipertrigliceridemia, acumulo de lipidos en tejido adiposo e h... more Dietas ricas en grasas desarrollan hipertrigliceridemia, acumulo de lipidos en tejido adiposo e higado entre otras alteraciones. Los Conjugados del Acido Linoleico (CLA) mejoran el perfil de lipidos plasmaticos y corporales, aunque tambien, generan ciertos efectos deletereos. Los tocoferoles y fitoesteroles presentes en los destilados de desodorizacion (DD) de aceites, han mostrado mejorar la dislipemia y reducir la produccion de citoquinas pro-inflamatorias.

Los acidos docosahexaenoico (DHA, 22:6 n-3) y araquidonico (AA, 20:4 n-6) determinan los niveles ... more Los acidos docosahexaenoico (DHA, 22:6 n-3) y araquidonico (AA, 20:4 n-6) determinan los niveles de mediadores lipidicos anti y proi-inflamatorios que son derivados de estos acidos grasos (AG) poliinsaturados (AGPI), por accion de enzimas, como las ciclooxigenasas (COX1 y COX2). El tipo de grasa dietaria, y algunos isomeros como los acidos grasos trans (AGt), podrian modular parametros relacionados a la inflamacion y al estres oxidativo.

Increasing polyunsaturated or long-chain fatty acids in meat for human consumption improves both ... more Increasing polyunsaturated or long-chain fatty acids in meat for human consumption improves both nutritional quality and consumer perception. The increase could occur through the addition of rich sources of omega-3 fatty acids (such as flaxseed or flaxseed oil) to the animal diet. The aim of this study was to evaluate the effects of dietary supplement with two presentations of flax (crushed seeds or oil) on the change of FA content in two cuts of caiman meat (tail and neck). We measured fatty profile in two different caiman meat cuts (neck and tail) from 30 animals (total length 96.7 ± 4.9 cm, snout-vent length 47.8 ± 3 cm, weight 4.2 ± 0.6 kg), raised in individual enclosures, fed three a week for 50 days with: crushed chicken head and a dry food formulated for these reptiles in a 70/30 ratio (C, n=10), control diet with 10% crushed flaxseed (FS, n=10), and control diet with 10% flaxseed oil (FO, n=10), while the remaining days animals were fed the control diet. Meats from animals ...

Mediterranean Journal of Nutrition and Metabolism, 2021

BACKGROUND: Arachidonic (20:4n-6) and docosahexaenoic (22:6n-3) acids interaction affects brain s... more BACKGROUND: Arachidonic (20:4n-6) and docosahexaenoic (22:6n-3) acids interaction affects brain structure and function. Unsaturated fatty acids (UFAs) generate oxygenated lipid-derived eicosanoids which modulate the inflammatory response. The presence of trans fatty acids (TFA) in neuronal membranes can favor to generation of pro-oxidant metabolites. OBJECTIVE: This study evaluated the effect of supplementation with TFA to diets containing different proportions of FA, on the oxidative stress (OS) generation and the inflammatory response in mice brain and liver. METHODS: CF1 mice were fed diets (16 weeks) with olive (O), corn (C) or rapeseed (R) oils. OS parameters and gene expression of some key liver and brain enzymes involved in OS production were evaluated. RESULTS: In brain and liver, lipoperoxidation was increased and catalase activity was decreased in C. In brain, glutathione was diminished by supplementation with TFA in all diets and histological sections showed lymphocytes i...

British Journal of Nutrition, 2020

Maternal nutritional programming by a high-fat (HF) diet is related to hepatic lipid accumulation... more Maternal nutritional programming by a high-fat (HF) diet is related to hepatic lipid accumulation and steatosis in offspring. Conjugated linoleic acid (CLA) might ameliorate impaired hepatic lipid homeostasis; therefore, the aim was to investigate the potential preventive effect of maternal CLA consumption on triacylglycerol (TAG) metabolism alterations induced by HF diets in adult male rat offspring receiving or not receiving CLA. Female Wistar rats were fed a control (C) diet, HF diet or HF diet supplemented with CLA (HF+CLA) for 4 weeks before mating and throughout pregnancy and lactation. After weaning, for 9 weeks, male offspring of C or HF rats continued with the same diets as their mothers (C/C or HF/HF groups, respectively), and male offspring of HF+CLA rats were fed HF or HF+CLA diets (HF+CLA/HF or HF+CLA/HF+CLA groups, respectively). Nutritional parameters, serum and liver TAG levels, the TAG secretion rate, and the activities as well as gene expression of key hepatic enzy...

European Journal of Lipid Science and Technology, 2019

This study aims to investigate the effect of conjugated linoleic acid (CLA) on nutritional parame... more This study aims to investigate the effect of conjugated linoleic acid (CLA) on nutritional parameters and triacylglycerol (TAG) regulation in male Wistar rats fed linoleic acid (LA)‐deprived (−LA) diets compared to LA‐enriched (+LA) diets. In both +LA and −LA groups, CLA are incorporated into the tissues, showing higher levels in the adipose tissue. However, different metabolic and nutritional effects are observed depending on the LA status. CLA markedly reduces fat depots in the −LA group, associated with an increased lipoprotein lipase (LPL) and lipogenic enzyme activities as compensatory mechanisms. Moreover, CLA restores the hepatic TAG levels in −LA animals, associated with a normalized triacylglycerol‐secretion rate (TAG‐SR), an increased lipogenic enzyme activity and higher mRNA levels of fatty acid synthase. Serum TAG levels are not affected by CLA in the +LA group. However, in the −LA group, CLA decreases the TAG levels associated with a reduced TAG‐SR and a higher adipose ...

The British journal of nutrition, 2017

Conjugated linoleic acid (CLA) might regulate the lipid depots in liver and adipose tissue. As th... more Conjugated linoleic acid (CLA) might regulate the lipid depots in liver and adipose tissue. As there is an association between maternal nutrition, fat depots and risk of offspring chronic disease, the aim was to investigate the effect of maternal CLA consumption on TAG regulation and some inflammatory parameters in adult male rat offspring receiving or not receiving CLA. Female Wistar rats were fed control (C) or CLA-supplemented (1 %, w/w) diets during 4 weeks before and throughout pregnancy and lactation. After weaning, male offspring of CLA rats were fed C or CLA diets (CLA/C and CLA/CLA groups, respectively), whereas C male rat offspring were fed a C diet (C/C group) for 9 weeks. Serum TAG levels were increased in the CLA/CLA and CLA/C groups, associated with a reduction of lipoprotein lipase activity and weights of adipose tissue. The liver TAG levels were decreased in the CLA/CLA group, related to a significant reduction of fatty acid synthase (FAS), acetyl-CoA carboxylase (AC...

British Journal of Nutrition, 2016

The aim of this study was to investigate the effects oftrans-fatty acids (TFA) on liver and serum... more The aim of this study was to investigate the effects oftrans-fatty acids (TFA) on liver and serum TAG regulation in mice fed diets containing different proportions ofn-3,n-6 andn-9 unsaturated fatty acids (UFA) from olive (O), maize (C) or rapeseed (R) oils partially substituted or not with TFA (Ot, Ct and Rt, respectively). Male CF1 mice were fed (30 d) one of these diets. The effects of the partial substitution (1 %, w/w) of different UFA with TFA on the activity and expression of hepatic enzymes involved in lipogenesis and fatty acids oxidation were evaluated, as well as their transcription factor expressions. Some of the mechanisms involved in the serum TAG regulation, hepatic VLDL rich in TAG (VLDL-TAG) secretion rate and lipoprotein lipase (LPL) activity were assessed. In liver, TFA induced an increase in TAG content in the Ot and Rt groups, and this effect was associated with an imbalance between lipogenesis andβ-oxidation. In the Ot group, exacerbated lipogenesis may be one ...

Nutritional neuroscience, Jan 21, 2016

The levels of docosahexaenoic acid (DHA, 22:6n-3) and arachidonic acid (AA, 20:4n-6) are critical... more The levels of docosahexaenoic acid (DHA, 22:6n-3) and arachidonic acid (AA, 20:4n-6) are critical for the normal structure and function of the brain. Trans fatty acids (TFA) and the source of the dietary fatty acids (FA) interfere with long-chain polyunsaturated fatty acids (LC-PUFA) biosynthesis. The aim of this study was to investigate the effect of TFA supplementation in diets containing different proportions of n-9, n-6, and n-3 FA on the brain FA profile, including the retention of TFA, LC-PUFA levels, and n-6/n-3 PUFA ratios. These parameters were also investigated in the liver, considering that LC-PUFA are mainly bioconverted from their dietary precursors in this tissue and transported by serum to the brain. Also, stearoyl-CoA desaturase-1 (SCD1) and sterol regulatory element-binding protein-1c (SREBP-1c) gene expressions were evaluated. Male CF1 mice were fed (16 weeks) diets containing different oils (olive, corn, and rapeseed) with distinct proportions of n-9, n-6, and n-3...

Nutricion hospitalaria, 2015

there is still little evidence on the metabolic trans fatty acids (TFA) effects at recommended fa... more there is still little evidence on the metabolic trans fatty acids (TFA) effects at recommended fat levels. to investigate the differential TFA effects on some nutritional parameters, TFA retention, and triacylglycerol (TAG) regulation in rats fed recommended and high-fat diets. male Wistar rats were fed (30 days) diets containing recommended (7%,w/w) or high-fat (20%,w/w) levels, supplemented or not with TFA (C7, C20, TFA7 and TFA20). TFA7 (vs.C7) rats showed an increased body weight associated with higher fat pads and liver and serum TAG. The hypertriacylglyceridaemia was related to a decreased muscle LPL activity, while the higher hepatic TAG content was associated with both an increased SREBP-1c gene expression and ACC activity, and a reduced CPT-Ia gene expression. The TFA20 diet did not potentiate the higher body weight, fat pads and TAG levels induced by the C20 diet. Although the hepatic TAG-secretion rate (TAG-SR) increased by TFA20 vs. C20, the same triacylglyceridaemia was...

Methods and Protocols

The Multidrug Resistance protein (ABCB1, MDR1) is involved in the transport of xenobiotics and an... more The Multidrug Resistance protein (ABCB1, MDR1) is involved in the transport of xenobiotics and antiretroviral drugs. Some variants of the ABCB1 gene are of clinical importance; among them, exon 12 (c.1236C>T, rs1128503), 21 (c.2677G>T/A, rs2032582), and 26 (c.3435C>T, rs1045642) have a high incidence in Caucasians. Several protocols have been used for genotyping the exon 21 variants, such as allele-specific PCR-RFLP using adapted primer to generate a digestion site for several enzymes and automatic sequencing to detect the SNVs, TaqMan Allele Discrimination assay and High-Resolution Melter analysis (HRMA). The aim was to describe a new approach to genotype the three variants c.2677G>T/A for the exon 21 doing only one PCR with the corresponding primers and the digestion of the PCR product with two restriction enzymes: BrsI to identify A allele and BseYI to differentiate between G or T. An improvement of this methodology was also described. The proposal technique here desc...

European Journal of Lipid Science and Technology, 2015

Given that the effects of trans fatty acids (TFA) may be associated with the type of TFA isomer a... more Given that the effects of trans fatty acids (TFA) may be associated with the type of TFA isomer and the proportion of n-3/n-6/n-9 fatty acids (FA), this work aimed to investigate the influence of different oils on the following parameters: content, incorporation, and retention of TFA; conversion of vaccenic acid (VA) to rumenic acid (RA) in different tissues; and hepatic output of TFA by VLDL. Another objective was to assess relative conversion rates of key FA and the potential alteration of FA composition induced by TFA in tissues. Male CF1 mice were fed (30-days) diets containing 7% olive, corn or rapeseed oils either supplemented with 0.75% of TFA or without added TFA. FA composition of liver, epididymal adipose tissue, gastrocnemius muscle, brain, and serum was assessed. With the exception of the brain, TFAs were incorporated into the analyzed tissues and serum. TFA retention and RA bioconversion from VA depended on the dietary unsaturated FA proportions. The higher levels of hepatic RA in the liver of mice fed an olive oilþTFA diet could be associated with a raised D9-desaturase index. The FA composition of tissues was scarcely modified by the consumption of partially hydrogenated vegetable oils containing similar proportions of t9-, t10-, and t11-18:1. Practical applications: The present study evaluates the interaction of hydrogenated vegetable oils with different edible oils and the impact on the FA profile and, specifically, TFA incorporation and retention in the tissues. Since the characterization of FA present in tissues might be determining the biological effects of edible fats, this study is relevant for elucidation of existing controversial findings associated with the intake of TFA and its effects on human metabolic alterations.

Biomedical Reports, 2020

In Argentina, porphyria cutanea tarda (PCT) is strongly associated with infection with human immu... more In Argentina, porphyria cutanea tarda (PCT) is strongly associated with infection with human immunodeficiency virus (HIV); however, whether the onset of this disease is associated with HIV infection and/or the antiretroviral therapy has not been determined. The ABCB1 gene variants c.1236C>T, c.2677G>T/A and c.3435C>T affect drug efflux. The GSTT1 null, GSTM1 null and GSTP1 (c.313A>G) gene variants alter Glutathione S-transferase (GST) activity, modifying the levels of xenobiotics. The aim of the present study was to evaluate the role of genetic variants in initiation of PCT and to analyze the genetic basis of the PCT-HIV association. Control individuals, and HIV, PCT and PCT-HIV patients were recruited, PCR-restriction fragment length polymorphism was used to genotype the ABCB1 and GSTP1 variants, and multiplex PCR was used to study the GSTM1 and GSTT1 variants. The high frequency of c.3435C>T (PCT and PCT-HIV) and c.1236C>T (PCT) suggested that the onset of PCT were not specifically related to HIV infection or antiretroviral therapy for these variants. c.2677G>T/A frequencies in the PCT-HIV patients were higher compared with the other groups, suggesting that a mechanism involving antiretroviral therapy served a role in this association. PCT-HIV patients also had a high frequency of GSTT1 null and low frequency for GSTM1 null variants; thus, the genetic basis for PCT onset may involve a combination between the absence of GSTT1 and the presence of GSTM1. In conclusion, genes encoding for proteins involved in the flow and metabolism of xenobiotics may influence the PCT-HIV association. The present study is the first to investigate the possible role of GST and ABCB1 gene variants in the triggering of PCT in HIV-infected individuals, to the best of our knowledge, and may provide novel insights into the molecular basis of the association between PCT and HIV.

Acta Bioquimica Clinica Latinoamericana, 2016

Fil: Rossetti, Maria Victoria. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficin... more Fil: Rossetti, Maria Victoria. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina

New Frontiers in Medicine and Medical Research Vol. 4, 2021

Porphyrias are a group of metabolic disorders due to alterations in heme biosynthesis. Porphyria ... more Porphyrias are a group of metabolic disorders due to alterations in heme biosynthesis. Porphyria Cutanea Tarda (PCT) is a hepatic cutaneous Porphyria resulting from an acquired or inherited deficiency of Uroporphyrinogen decarboxylase. Triggering factors are involved in the onset of this disease. In Argentina, PCT is strongly associated to infection with human immunodeficiency virus (HIV); however, whether the onset of this disease is associated with HIV infection and/or the antiretroviral therapy has not been determined. Our investigation was focused to study the involvement of genetic variants in the development of Porphyrias. In this work, the aim was to evaluate the role of ABCB1 and GST genetic variants in the association between PCT and HIV. This article summarized the main reports about the role of drug metabolism in the onset of PCT, in particular those related with cytochrome P-450 gene variants. Moreover, we extensively described our results about the involvement of ABCB1 transporter and Glutathione S-transferases (GSTs) variants in the association PCT-HIV. We centered our investigation on the ABCB1 gene variants: Exon 12 (rs1128503, NM_000927.5: c.1236C>T), exon 21 (rs2032582, NM_000927.5: and c.2677G>T/A) and exon 26 (rs1045642, NM_000927.5: and c.3435C>T), that affect drug efflux. The genotypification of GSTT1 null, GSTM1 null and GSTP1 (rs1695, NM_000852.4: and c.313A>G), gene variants that alter activity, modifying xenobiotics levels was also analyzed. The high frequency of c.3435C>T (PCT and PCT-HIV) and c.1236C>T (PCT) suggested that the onset of PCT was not specifically related to HIV infection or antiretroviral therapy for these variants. c.2677G>T/A frequencies in the PCT-HIV patients were higher compared with the other groups, suggesting that a mechanism involving antiretroviral therapy had a role in this association. PCT-HIV patients also had a high frequency of GSTT1 null and low frequency for GSTM1 null variants; thus, the genetic basis for PCT onset may involve a combination between the absence of GSTT1 and the presence of GSTM1. When the gene variants studied were analyzed in a whole, PCT-HIV patients had more risk alleles than Controls, PCT or HIV groups. In conclusion, genes encoding for proteins involved in the flow and metabolism of xenobiotics may influence the PCT-HIV association, providing novel insights into the molecular basis of the association between PCT and HIV.