johan selmer - Academia.edu (original) (raw)
Papers by johan selmer
Journal of Biological Chemistry, Dec 1, 1990
Springer eBooks, 1985
Some methodological possibilities for the analysis of membrane proteins based on different types ... more Some methodological possibilities for the analysis of membrane proteins based on different types of antibody are described. Detergent immunoelectrophoresis (D-IE) is surveyed as a technique which works with polyspecific antibodies (Ab’s), and immunoblotting as one which functions with oligo- and mono-specific Ab’s. Finally, the application of monoclonal Ab’s in immunosorbent chromatography and ELISA techniques is shown.
Current Pharmaceutical Biotechnology, Jul 1, 2000
PubMed, Aug 1, 1985
A generally applicable approach to the preparative isolation of amphiphilic membrane proteins tha... more A generally applicable approach to the preparative isolation of amphiphilic membrane proteins that follow the Triton X-114 phase during a temperature-dependent phase separation is described. The phase separations were performed direct on whole blood and a 650-fold purification of human erythrocyte membrane acetylcholinesterase (AchE) was obtained. Thus, 0.2 mg enzyme was isolated per 1 liter of blood, with a specific activity of 13 IU/mg, the major contaminants being glycophorin and hemoglobin. The protein material was isolated from the detergent phase by Cu2+ chelate chromatography. This material was used to raise monoclonal anti-AchE antibodies which, when applied to immunosorbent chromatography of washed Triton X-100-lysed erythrocytes in one step, allowed a 246,000-fold purification of AchE with a yield of 88% and a specific activity of 3800 IU/mg.
Clinical Endocrinology, 1982
SUMMARYHypothalamic–pituitary–adrenocortical (HPA) function was assessed during normal daily work... more SUMMARYHypothalamic–pituitary–adrenocortical (HPA) function was assessed during normal daily work and family life in fifteen male brewery workers with a daily alcohol intake of 100 g or more for at least 10 years. Free urinary cortisol was determined during three baseline days and for 3 days during administration of dexamethasone 0·5 mg 6‐hourly. All subjects had normal basal urinary cortisol excretion and all except one suppressed normally upon dexame our subjects had Cushingoid‐like moon face and two of these also alo hump. There was no correlation between free urinary cortisol or dexamethasone suppression and the Cushingoid stigmata. These results suggest that chronic alcohol intake per se does not influence HPA‐function.
Fems Microbiology Letters, Aug 1, 1985
Fems Microbiology Letters, Aug 1, 1985
British Journal of Dermatology, Jan 9, 2016
L'invention concerne l'utilisation d'un medicament constituant un traitement de survi... more L'invention concerne l'utilisation d'un medicament constituant un traitement de survie pour des patients gravement atteints ou pour des patients souffrant du syndrome de reponse inflammatoire systemique (SIRS). L'invention concerne egalement une methode therapeutique. Le medicament selon l'invention contient un compose GLP-1 qui regule de maniere efficace le taux de glycemie.
L'invention concerne des procedes et des utilisations pour traiter des complications diabetiq... more L'invention concerne des procedes et des utilisations pour traiter des complications diabetiques tardives, consistant a administrer un compose GLP-1 et un modulateur de complications diabetiques.
Scandinavian Journal of Clinical and Laboratory Investigation, 1984
Plasma and urine fibronectin concentration was determined by electroimmunoassay and ELISA-method ... more Plasma and urine fibronectin concentration was determined by electroimmunoassay and ELISA-method in patients who received renal transplantation. The plasma fibronectin concentration decreased both after the transplantation, in relation to the acute rejection of the graft, and in the relation to immunosuppressive therapy. Urine fibronectin excretion increased in relation to the kidney transplantation and acute rejection of the graft. In association with improved kidney function, the urine fibronectin excretion decreased. It is suggested that it might be of clinical importance to determine the excretion of fibronectin into the urine in patients undergoing kidney transplantation.
Acta pathologica, microbiologica et immunologica Scandinavica, Aug 15, 2009
Two monoclonal antibodies against filamentous haemagglutinin (F-HA) from Bordetella have been pro... more Two monoclonal antibodies against filamentous haemagglutinin (F-HA) from Bordetella have been produced. In immunoblotting of a SDS-polyacrylamide gel electrophoresis (SDS-PAGE) of high ionic strength extracts of Bordetella pertussis, the two monoclonal antibodies both stained several high molecular weight bands. The two monoclonal antibodies were used for immuno-affinity column one-step purification of F-HA from high-ionic-strength extracts of Bordetella pertussis. In gradient SDS-PAGE, the eluted F-HA is present as a major double band with a molecular weight of approximately 240,000 daltons and several minor bands with molecular weights down to 90,000 daltons. The monoclonal antibodies were used in a monoclonal antibody catching enzyme linked immunosorbent assay (ELISA) for sensitive and specific detection of F-HA. This ELISA system proved valuable in optimizing the elution conditions for the monoclonal affinity columns.
Journal of The European Academy of Dermatology and Venereology, Nov 5, 2021
BackgroundPlaque psoriasis is a common, chronic and relapsing inflammatory skin disease clinicall... more BackgroundPlaque psoriasis is a common, chronic and relapsing inflammatory skin disease clinically characterized by erythema and scaling desquamation. As over 90% of psoriasis patients benefit from topical therapies, local treatments continue to play an eminent role in management strategies. One such topical treatment is the fixed dose combination of calcipotriol (CAL) and betamethasone dipropionate (BDP).ObjectivesPooled analysis of two different phase 3 clinical trails to compare superiority regarding efficacy, safety and quality of life (QoL) between CAL/BDP PAD‐cream and CAL/BDP TS.MethodsThe data from two phase 3, multicentre, randomized, investigator‐blind, active and vehicle‐controlled trials enrolling patients with psoriasis were pooled and analysed. Investigational products included a CAL/BDP cream based on PAD™ Technology (PAD‐cream) designed for high skin penetration and increased patient preference, an active control (marketed CAL/BDP topical suspension/gel, in the following abbreviated as CAL/BDP TS) and cream vehicle, which were applied once daily for 8 weeks.ResultsEfficacy and safety of the novel CAL/BDP PAD‐cream formulation for the topical treatment of psoriasis demonstrated superiority for all efficacy end points after 8 weeks of treatment. PGA treatment success for CAL/BDP PAD‐cream (43.2%) was greater than CAL/BDP TS (31.9%; P < 0.0001), the mean per cent reduction in mPASI for CAL/BDP PAD‐cream was 64.6% compared to 56.4% for CAL/BDP TS (P < 0.0001) and DLQI 0/1 was obtained by 43.8% in the CAL/BDP PAD‐cream group versus 34.2% in the CAL/BDP TS group (P = 0.0005). There was no adverse drug reaction reported with a frequency of >1%, associated with the CAL/BDP PAD‐cream.ConclusionsThe novel fixed dose combination CAL/BDP PAD‐cream offers greater efficacy, superior patient QoL and equivalent favourable safety for the topical treatment of psoriasis, in comparison to the currently available topical suspension/gel.
Journal of the European Academy of Dermatology and Venereology
Four posters about the novel, fixed‐dose calcipotriol and betamethasone dipropionate cream (CAL/B... more Four posters about the novel, fixed‐dose calcipotriol and betamethasone dipropionate cream (CAL/BDP cream) based on Poly‐Aphron Dispersion (PAD) Technology were presented at the 30th European Academy of Dermatology and Venereology (EADV) Congress 2021 and are summarized here. CAL/BDP cream was compared in two randomized, phase 3 trials to vehicle and active comparator (CAL/BDP gel/topical suspension [TS]) in adults with plaque psoriasis (NCT03802344 and NCT03308799). Pooled data from both trials demonstrated significant greater efficacy in favour of CAL/BDP cream for all efficacy endpoints, including PGA treatment success, mPASI, and mPASI75 compared to CAL/BDP gel/TS. CAL/BDP cream was well tolerated and comparable to CAL/BDP gel/TS with no adverse drug reactions with a frequency >1%. In the NCT03308799 study, CAL/BDP cream demonstrated a substantial improvement in the proportion of participants achieving a minimum 4‐point improvement on the peak pruritus numeric rating scale (N...
SKIN The Journal of Cutaneous Medicine, 2022
Journal of Biological Chemistry, Dec 1, 1990
Springer eBooks, 1985
Some methodological possibilities for the analysis of membrane proteins based on different types ... more Some methodological possibilities for the analysis of membrane proteins based on different types of antibody are described. Detergent immunoelectrophoresis (D-IE) is surveyed as a technique which works with polyspecific antibodies (Ab’s), and immunoblotting as one which functions with oligo- and mono-specific Ab’s. Finally, the application of monoclonal Ab’s in immunosorbent chromatography and ELISA techniques is shown.
Current Pharmaceutical Biotechnology, Jul 1, 2000
PubMed, Aug 1, 1985
A generally applicable approach to the preparative isolation of amphiphilic membrane proteins tha... more A generally applicable approach to the preparative isolation of amphiphilic membrane proteins that follow the Triton X-114 phase during a temperature-dependent phase separation is described. The phase separations were performed direct on whole blood and a 650-fold purification of human erythrocyte membrane acetylcholinesterase (AchE) was obtained. Thus, 0.2 mg enzyme was isolated per 1 liter of blood, with a specific activity of 13 IU/mg, the major contaminants being glycophorin and hemoglobin. The protein material was isolated from the detergent phase by Cu2+ chelate chromatography. This material was used to raise monoclonal anti-AchE antibodies which, when applied to immunosorbent chromatography of washed Triton X-100-lysed erythrocytes in one step, allowed a 246,000-fold purification of AchE with a yield of 88% and a specific activity of 3800 IU/mg.
Clinical Endocrinology, 1982
SUMMARYHypothalamic–pituitary–adrenocortical (HPA) function was assessed during normal daily work... more SUMMARYHypothalamic–pituitary–adrenocortical (HPA) function was assessed during normal daily work and family life in fifteen male brewery workers with a daily alcohol intake of 100 g or more for at least 10 years. Free urinary cortisol was determined during three baseline days and for 3 days during administration of dexamethasone 0·5 mg 6‐hourly. All subjects had normal basal urinary cortisol excretion and all except one suppressed normally upon dexame our subjects had Cushingoid‐like moon face and two of these also alo hump. There was no correlation between free urinary cortisol or dexamethasone suppression and the Cushingoid stigmata. These results suggest that chronic alcohol intake per se does not influence HPA‐function.
Fems Microbiology Letters, Aug 1, 1985
Fems Microbiology Letters, Aug 1, 1985
British Journal of Dermatology, Jan 9, 2016
L'invention concerne l'utilisation d'un medicament constituant un traitement de survi... more L'invention concerne l'utilisation d'un medicament constituant un traitement de survie pour des patients gravement atteints ou pour des patients souffrant du syndrome de reponse inflammatoire systemique (SIRS). L'invention concerne egalement une methode therapeutique. Le medicament selon l'invention contient un compose GLP-1 qui regule de maniere efficace le taux de glycemie.
L'invention concerne des procedes et des utilisations pour traiter des complications diabetiq... more L'invention concerne des procedes et des utilisations pour traiter des complications diabetiques tardives, consistant a administrer un compose GLP-1 et un modulateur de complications diabetiques.
Scandinavian Journal of Clinical and Laboratory Investigation, 1984
Plasma and urine fibronectin concentration was determined by electroimmunoassay and ELISA-method ... more Plasma and urine fibronectin concentration was determined by electroimmunoassay and ELISA-method in patients who received renal transplantation. The plasma fibronectin concentration decreased both after the transplantation, in relation to the acute rejection of the graft, and in the relation to immunosuppressive therapy. Urine fibronectin excretion increased in relation to the kidney transplantation and acute rejection of the graft. In association with improved kidney function, the urine fibronectin excretion decreased. It is suggested that it might be of clinical importance to determine the excretion of fibronectin into the urine in patients undergoing kidney transplantation.
Acta pathologica, microbiologica et immunologica Scandinavica, Aug 15, 2009
Two monoclonal antibodies against filamentous haemagglutinin (F-HA) from Bordetella have been pro... more Two monoclonal antibodies against filamentous haemagglutinin (F-HA) from Bordetella have been produced. In immunoblotting of a SDS-polyacrylamide gel electrophoresis (SDS-PAGE) of high ionic strength extracts of Bordetella pertussis, the two monoclonal antibodies both stained several high molecular weight bands. The two monoclonal antibodies were used for immuno-affinity column one-step purification of F-HA from high-ionic-strength extracts of Bordetella pertussis. In gradient SDS-PAGE, the eluted F-HA is present as a major double band with a molecular weight of approximately 240,000 daltons and several minor bands with molecular weights down to 90,000 daltons. The monoclonal antibodies were used in a monoclonal antibody catching enzyme linked immunosorbent assay (ELISA) for sensitive and specific detection of F-HA. This ELISA system proved valuable in optimizing the elution conditions for the monoclonal affinity columns.
Journal of The European Academy of Dermatology and Venereology, Nov 5, 2021
BackgroundPlaque psoriasis is a common, chronic and relapsing inflammatory skin disease clinicall... more BackgroundPlaque psoriasis is a common, chronic and relapsing inflammatory skin disease clinically characterized by erythema and scaling desquamation. As over 90% of psoriasis patients benefit from topical therapies, local treatments continue to play an eminent role in management strategies. One such topical treatment is the fixed dose combination of calcipotriol (CAL) and betamethasone dipropionate (BDP).ObjectivesPooled analysis of two different phase 3 clinical trails to compare superiority regarding efficacy, safety and quality of life (QoL) between CAL/BDP PAD‐cream and CAL/BDP TS.MethodsThe data from two phase 3, multicentre, randomized, investigator‐blind, active and vehicle‐controlled trials enrolling patients with psoriasis were pooled and analysed. Investigational products included a CAL/BDP cream based on PAD™ Technology (PAD‐cream) designed for high skin penetration and increased patient preference, an active control (marketed CAL/BDP topical suspension/gel, in the following abbreviated as CAL/BDP TS) and cream vehicle, which were applied once daily for 8 weeks.ResultsEfficacy and safety of the novel CAL/BDP PAD‐cream formulation for the topical treatment of psoriasis demonstrated superiority for all efficacy end points after 8 weeks of treatment. PGA treatment success for CAL/BDP PAD‐cream (43.2%) was greater than CAL/BDP TS (31.9%; P < 0.0001), the mean per cent reduction in mPASI for CAL/BDP PAD‐cream was 64.6% compared to 56.4% for CAL/BDP TS (P < 0.0001) and DLQI 0/1 was obtained by 43.8% in the CAL/BDP PAD‐cream group versus 34.2% in the CAL/BDP TS group (P = 0.0005). There was no adverse drug reaction reported with a frequency of >1%, associated with the CAL/BDP PAD‐cream.ConclusionsThe novel fixed dose combination CAL/BDP PAD‐cream offers greater efficacy, superior patient QoL and equivalent favourable safety for the topical treatment of psoriasis, in comparison to the currently available topical suspension/gel.
Journal of the European Academy of Dermatology and Venereology
Four posters about the novel, fixed‐dose calcipotriol and betamethasone dipropionate cream (CAL/B... more Four posters about the novel, fixed‐dose calcipotriol and betamethasone dipropionate cream (CAL/BDP cream) based on Poly‐Aphron Dispersion (PAD) Technology were presented at the 30th European Academy of Dermatology and Venereology (EADV) Congress 2021 and are summarized here. CAL/BDP cream was compared in two randomized, phase 3 trials to vehicle and active comparator (CAL/BDP gel/topical suspension [TS]) in adults with plaque psoriasis (NCT03802344 and NCT03308799). Pooled data from both trials demonstrated significant greater efficacy in favour of CAL/BDP cream for all efficacy endpoints, including PGA treatment success, mPASI, and mPASI75 compared to CAL/BDP gel/TS. CAL/BDP cream was well tolerated and comparable to CAL/BDP gel/TS with no adverse drug reactions with a frequency >1%. In the NCT03308799 study, CAL/BDP cream demonstrated a substantial improvement in the proportion of participants achieving a minimum 4‐point improvement on the peak pruritus numeric rating scale (N...
SKIN The Journal of Cutaneous Medicine, 2022