john jerome - Academia.edu (original) (raw)
Papers by john jerome
Pain physician
Pain interventionists can interrupt pain through anesthetic blockade of neural transmission to vi... more Pain interventionists can interrupt pain through anesthetic blockade of neural transmission to virtually any part of the body. Temporary pain relief can be achieved by the direct application of targeted anesthetic. Diagnostically, nerve blocks help identify specific pain generators, refine differential diagnosis, and disrupt the neural transmission mechanisms to stop pain generation peripherally. This study of patients with chronic spine pain was conducted to test the hypothesis that decreasing pain through interventional techniques coupled with cognitive motivational counseling can be highly effective in reducing chronic pain interference, reliance on prescription opioids, and enhancing overall function and quality of life. Retrospective case series. Rehabilitation center. This study involved a retrospective cohort of 78 consecutive patients with spine pain that underwent interventional procedures and cognitive motivational counseling, as well as a comparison group of 77 consecutiv...
BMC Genomics, 2016
Background: ChIP-seq is the primary technique used to investigate genome-wide protein-DNA interac... more Background: ChIP-seq is the primary technique used to investigate genome-wide protein-DNA interactions. As part of this procedure, immunoprecipitated DNA must undergo "library preparation" to enable subsequent highthroughput sequencing. To facilitate the analysis of biopsy samples and rare cell populations, there has been a recent proliferation of methods allowing sequencing library preparation from low-input DNA amounts. However, little information exists on the relative merits, performance, comparability and biases inherent to these procedures. Notably, recently developed single-cell ChIP procedures employing microfluidics must also employ library preparation reagents to allow downstream sequencing. Results: In this study, seven methods designed for low-input DNA/ChIP-seq sample preparation (Accel-NGS® 2S, Bowman-method, HTML-PCR, SeqPlex™, DNA SMART™, TELP and ThruPLEX®) were performed on five replicates of 1 ng and 0.1 ng input H3K4me3 ChIP material, and compared to a "gold standard" reference PCR-free dataset. The performance of each method was examined for the prevalence of unmappable reads, amplification-derived duplicate reads, reproducibility, and for the sensitivity and specificity of peak calling. Conclusions: We identified consistent high performance in a subset of the tested reagents, which should aid researchers in choosing the most appropriate reagents for their studies. Furthermore, we expect this work to drive future advances by identifying and encouraging use of the most promising methods and reagents. The results may also aid judgements on how comparable are existing datasets that have been prepared with different sample library preparation reagents.
The Journal of the American Osteopathic Association, Nov 1, 2010
A B S T R A C T Tooth movement results from alveolar bone resorption/deposition following applica... more A B S T R A C T Tooth movement results from alveolar bone resorption/deposition following application of orthodontic forces, and root resorption can be an undesirable complication associated with this process. No treatment for external root resorption is available to date. Objective: To determine if COX-2 inhibitors like Celebrex are effec- tive in protecting root resorption associated with orthodontic
Aps March Meeting Abstracts, Mar 1, 2004
Thin films of EVA or PE and dPS were spun cast onto Si wafers and crystallized in supercritical c... more Thin films of EVA or PE and dPS were spun cast onto Si wafers and crystallized in supercritical carbon dioxide at pressures and temperatures corresponding to the density fluctuation ridge^1 or thermally annealed using standard protocols. The morphology composition and melting points of the films were studied using scanning force microscopy and imaging time-of-flight secondary ion mass spectrometry as function
The Journal of the American Osteopathic Association, 2012
The potency of touch in osteopathic manipulative treatment (OMT) is physically realized within th... more The potency of touch in osteopathic manipulative treatment (OMT) is physically realized within the musculoskeletal, immune, nervous, and endocrine systems. Psychologically, touch supports a verbal and tactile interaction that is both diagnostic and therapeutic. Touch is a 2-way street that adds meaning and depth to the patient-physician experience. The relationship between touching and being touched offers a potentially powerful and intense deepening of the patient-physician relationship that emerges within the palpatory examination and treatment. Empathic communication, through word or deed, allows a therapeutic, synchronized healing to occur. In the present article, the authors provide a rationale to sensitize and invigorate osteopathic physicians to routinely evaluate and treat patients using their skillful touch.
Journal of the California Dental Association, 2005
Tooth movement results from alveolar bone resorption/deposition following application of orthodon... more Tooth movement results from alveolar bone resorption/deposition following application of orthodontic forces, and root resorption can be an undesirable complication associated with this process. No treatment for external root resorption is available to date. To determine if COX-2 inhibitors like Celebrex are effective in protecting root resorption associated with orthodontic forces. A force of 80 grams was applied to the left maxillary first molars of 7-week-old female Wistar rats using nickel titanium closed coil springs attached to the cervical area of the incisors with 0.010 stainless-steel ligature wires. Twenty animals were divided into three experimental groups: one receiving no treatment, the second receiving 25mg/kg, and the third receiving 50 mg/kg of celecoxib (Celebrex) in their drinking water. Rats were maintained on a soft diet and euthanized two weeks after initial placement of the force. Paraffin-embedded sections of the right (control) and left (experimental) maxillae...
Pain Forum, 1995
he modern history of pain theory building began in the early 19th century when physiology emerged... more he modern history of pain theory building began in the early 19th century when physiology emerged as a true experimental science. These early studies provided observable objective evidence that Decartes' straight through pain transmission pain theory was over simplistic and flawed. Since that time, rather than focusing on the interaction of cognitive, affective, and behavior processes, psychophysiologists have instead focused on mapping and describing pain transmission pathways. Consequently, the evolution of pain theory has closely paralleled our expanding knowledge of sensory physiology. From their physical observations and measurements we have had a series of pain theories (i.e., specificity theory, summation theory, pattern theory, the "forth theory of pain," sensory interaction theory)':" and finally the gate control theory of pain,9,10 which blended the scientifically agreed on elements of the earlier transmission theories, placing emphasis on signal modulation at the spinal cord and "central control processes." Central control processes initially were the "black box" in a physical wiring diagram in which were housed unobserved latent variables, such as attention, cognition, affect, appraisal, personality traits, learning, social context, cultural background, information processing style, childhood trauma, etc. 2,5,10 The organization, let alone the function and reciprocal causality among these central control facets, must be at least as complex as that of the physioloqical pain transmission systems from which we have drawn our theories." Structural equation modeling (SEM) may now be giving us the tools to describe the ,relationships among central control processes.
Pain physician
Pain interventionists can interrupt pain through anesthetic blockade of neural transmission to vi... more Pain interventionists can interrupt pain through anesthetic blockade of neural transmission to virtually any part of the body. Temporary pain relief can be achieved by the direct application of targeted anesthetic. Diagnostically, nerve blocks help identify specific pain generators, refine differential diagnosis, and disrupt the neural transmission mechanisms to stop pain generation peripherally. This study of patients with chronic spine pain was conducted to test the hypothesis that decreasing pain through interventional techniques coupled with cognitive motivational counseling can be highly effective in reducing chronic pain interference, reliance on prescription opioids, and enhancing overall function and quality of life. Retrospective case series. Rehabilitation center. This study involved a retrospective cohort of 78 consecutive patients with spine pain that underwent interventional procedures and cognitive motivational counseling, as well as a comparison group of 77 consecutiv...
BMC Genomics, 2016
Background: ChIP-seq is the primary technique used to investigate genome-wide protein-DNA interac... more Background: ChIP-seq is the primary technique used to investigate genome-wide protein-DNA interactions. As part of this procedure, immunoprecipitated DNA must undergo "library preparation" to enable subsequent highthroughput sequencing. To facilitate the analysis of biopsy samples and rare cell populations, there has been a recent proliferation of methods allowing sequencing library preparation from low-input DNA amounts. However, little information exists on the relative merits, performance, comparability and biases inherent to these procedures. Notably, recently developed single-cell ChIP procedures employing microfluidics must also employ library preparation reagents to allow downstream sequencing. Results: In this study, seven methods designed for low-input DNA/ChIP-seq sample preparation (Accel-NGS® 2S, Bowman-method, HTML-PCR, SeqPlex™, DNA SMART™, TELP and ThruPLEX®) were performed on five replicates of 1 ng and 0.1 ng input H3K4me3 ChIP material, and compared to a "gold standard" reference PCR-free dataset. The performance of each method was examined for the prevalence of unmappable reads, amplification-derived duplicate reads, reproducibility, and for the sensitivity and specificity of peak calling. Conclusions: We identified consistent high performance in a subset of the tested reagents, which should aid researchers in choosing the most appropriate reagents for their studies. Furthermore, we expect this work to drive future advances by identifying and encouraging use of the most promising methods and reagents. The results may also aid judgements on how comparable are existing datasets that have been prepared with different sample library preparation reagents.
The Journal of the American Osteopathic Association, Nov 1, 2010
A B S T R A C T Tooth movement results from alveolar bone resorption/deposition following applica... more A B S T R A C T Tooth movement results from alveolar bone resorption/deposition following application of orthodontic forces, and root resorption can be an undesirable complication associated with this process. No treatment for external root resorption is available to date. Objective: To determine if COX-2 inhibitors like Celebrex are effec- tive in protecting root resorption associated with orthodontic
Aps March Meeting Abstracts, Mar 1, 2004
Thin films of EVA or PE and dPS were spun cast onto Si wafers and crystallized in supercritical c... more Thin films of EVA or PE and dPS were spun cast onto Si wafers and crystallized in supercritical carbon dioxide at pressures and temperatures corresponding to the density fluctuation ridge^1 or thermally annealed using standard protocols. The morphology composition and melting points of the films were studied using scanning force microscopy and imaging time-of-flight secondary ion mass spectrometry as function
The Journal of the American Osteopathic Association, 2012
The potency of touch in osteopathic manipulative treatment (OMT) is physically realized within th... more The potency of touch in osteopathic manipulative treatment (OMT) is physically realized within the musculoskeletal, immune, nervous, and endocrine systems. Psychologically, touch supports a verbal and tactile interaction that is both diagnostic and therapeutic. Touch is a 2-way street that adds meaning and depth to the patient-physician experience. The relationship between touching and being touched offers a potentially powerful and intense deepening of the patient-physician relationship that emerges within the palpatory examination and treatment. Empathic communication, through word or deed, allows a therapeutic, synchronized healing to occur. In the present article, the authors provide a rationale to sensitize and invigorate osteopathic physicians to routinely evaluate and treat patients using their skillful touch.
Journal of the California Dental Association, 2005
Tooth movement results from alveolar bone resorption/deposition following application of orthodon... more Tooth movement results from alveolar bone resorption/deposition following application of orthodontic forces, and root resorption can be an undesirable complication associated with this process. No treatment for external root resorption is available to date. To determine if COX-2 inhibitors like Celebrex are effective in protecting root resorption associated with orthodontic forces. A force of 80 grams was applied to the left maxillary first molars of 7-week-old female Wistar rats using nickel titanium closed coil springs attached to the cervical area of the incisors with 0.010 stainless-steel ligature wires. Twenty animals were divided into three experimental groups: one receiving no treatment, the second receiving 25mg/kg, and the third receiving 50 mg/kg of celecoxib (Celebrex) in their drinking water. Rats were maintained on a soft diet and euthanized two weeks after initial placement of the force. Paraffin-embedded sections of the right (control) and left (experimental) maxillae...
Pain Forum, 1995
he modern history of pain theory building began in the early 19th century when physiology emerged... more he modern history of pain theory building began in the early 19th century when physiology emerged as a true experimental science. These early studies provided observable objective evidence that Decartes' straight through pain transmission pain theory was over simplistic and flawed. Since that time, rather than focusing on the interaction of cognitive, affective, and behavior processes, psychophysiologists have instead focused on mapping and describing pain transmission pathways. Consequently, the evolution of pain theory has closely paralleled our expanding knowledge of sensory physiology. From their physical observations and measurements we have had a series of pain theories (i.e., specificity theory, summation theory, pattern theory, the "forth theory of pain," sensory interaction theory)':" and finally the gate control theory of pain,9,10 which blended the scientifically agreed on elements of the earlier transmission theories, placing emphasis on signal modulation at the spinal cord and "central control processes." Central control processes initially were the "black box" in a physical wiring diagram in which were housed unobserved latent variables, such as attention, cognition, affect, appraisal, personality traits, learning, social context, cultural background, information processing style, childhood trauma, etc. 2,5,10 The organization, let alone the function and reciprocal causality among these central control facets, must be at least as complex as that of the physioloqical pain transmission systems from which we have drawn our theories." Structural equation modeling (SEM) may now be giving us the tools to describe the ,relationships among central control processes.