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Papers by junyan Li

Journal of Neuroinflammation

Background The activation of NOD-like receptor protein 3 (NLRP3) inflammasome-dependent pyroptosi... more Background The activation of NOD-like receptor protein 3 (NLRP3) inflammasome-dependent pyroptosis has been shown to play a vital role in the pathology of manganese (Mn)-induced neurotoxicity. Sodium para-aminosalicylic acid (PAS-Na) has a positive effect on the treatment of manganism. However, the mechanism is still unclear. We hypothesized that PAS-Na might act through NLRP3. Methods The microglial cell line BV2 and male Sprague-Dawley rats were used to investigate the impacts of PAS-Na on Mn-induced NLRP3 inflammasome-dependent pyroptosis. The related protein of the NF-κB pathway and NLRP3-inflammasome-dependent pyroptosis was detected by western blot. The reactive oxygen species and mitochondrial membrane potential were detected by immunofluorescence staining and flow cytometry. The activation of microglia and the gasdermin D (GSDMD) were detected by immunofluorescence staining. Results Our results showed that Mn treatment induced oxidative stress and activated the NF-κB pathway...

Journal of Shenzhen University Science and Engineering

Cells

Epigenetic regulation plays an important role in the development and progression of nasopharyngea... more Epigenetic regulation plays an important role in the development and progression of nasopharyngeal carcinoma (NPC), but the epigenetic mechanisms underlying NPC metastasis remain poorly understood. Here, we demonstrate that hypermethylation of the UCHL1 promoter leads to its downregulation in NPC. Restoration of UCHL1 inhibited the migration and invasion of NPC cells in vitro and in vivo, and knockdown of UCHL1 promoted NPC cell migration and invasion in vitro and in vivo. Importantly, we found that UCHL1 interacts with CTTN, and may function as a ligase promoting CTTN degradation by increasing K48-linked ubiquitination of CTTN. Additionally, restoration of CTTN in NPC cells that overexpressed UCHL1 rescued UCHL1 suppressive effects on NPC cell migration and invasion, which indicated that CTTN is a functional target of UCHL1 in NPC. Our findings revealed that UCHL1 acts as a tumor suppressor gene in NPC and thus provided a novel therapeutic target for NPC treatment.

Applied Physics Letters

In this paper, we have presented the dispersion curve of Lamb waves in an aluminum plate with a p... more In this paper, we have presented the dispersion curve of Lamb waves in an aluminum plate with a periodic rectangular corrugation experimentally. A mode selection method was applied to generate Lamb waves in the region of the corrugated periodic structure using a moving continuous wave laser, and the signal can be detected at any point in the excitation region. The relationship between the phase velocity and the frequency is observed. It is found that the intensity distribution of the dispersion curves in the experimental results is related to the slope of the dispersion curves as well as the selection of the detection point. Vibrations at a series of locations were detected, reflecting the change in energy that Lamb waves undergo in the process of propagation through the periodic structure, which is different from the existing method of single-point excitation and detection. The spatial distribution of the mode amplitude at the resonances points, in particular induced by the Bragg reflection, has been studied.

Cancer Medicine

This is an open access article under the terms of the Creat ive Commo ns Attri bution License, wh... more This is an open access article under the terms of the Creat ive Commo ns Attri bution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Bioscience reports, Jan 25, 2018

Long non-coding RNA (lncRNA) SNHG14 is previously found to be overexpressed in several types of c... more Long non-coding RNA (lncRNA) SNHG14 is previously found to be overexpressed in several types of cancers. However, the clinical significance and biological function of SNHG14 in non-small cell lung cancer (NSCLC) are still elusive. In the present study, we found that SNHG14 was aberrantly upregulated in NSCLC tissues from patients and cell lines compared to their normal counterparts. Increased SNHG14 expression was closely associated with aggressive tumor progression and poor clinical outcome of NSCLC patients. Knockdown of SNHG14 inhibited NSCLC cell proliferation through inducing cell cycle arrest and apoptosis, whereas SNHG14 overexpression exerted the opposite effects. Animal experiment further revealed that downregulated SNHG14 greatly inhibited NSCLC tumor growth in vivo. Further studies demonstrated that SNHG14 might serve as a competing endogenous RNA (ceRNA) by sponging miR-340 in NSCLC cells. Taken together, our study demonstrated that SNHG14/miR-340 axis might play a novel...

Virology, May 6, 2018

Porcine reproductive and respiratory syndrome virus (PRRSV) causes inflammatory injuries in infec... more Porcine reproductive and respiratory syndrome virus (PRRSV) causes inflammatory injuries in infected pigs. PRRSV induces secretion of high mobility group box 1 (HMGB1) that enhances inflammatory response. However, the mechanism of PRRSV-induced HMGB1 secretion is unknown. Here, we discovered PRRSV induced HMGB1 secretion via activating protein kinase C-delta (PKCδ). HMGB1 secretion was positively correlated with PKCδ activation in PRRSV-infected cells in a dose and time-dependent manner. Suppression of PKCδ with inhibitor and siRNA significantly blocked PRRSV-induced HMGB1 translocation and secretion, which indicates PKCδ activation is essential for the PRRSV-mediated HMGB1 secretion. In addition, PKCδ knockdown in PRRSV-infected cells led to downregulation of inflammatory cytokines, including IL-1beta and IL-6. Moreover, PRRSV E and pORF5a proteins were found to activate PKCδ and consequent HMGB1 secretion. These results demonstrate PRRSV activates PKCδ to induce HMGB1 secretion vi...

Annals of biomedical engineering, Jan 22, 2018

Osteoporosis is the most common bone disease. However, the mechanism of osteoporosis-induced alte... more Osteoporosis is the most common bone disease. However, the mechanism of osteoporosis-induced alterations in bone is still unclear. The aim of this study was to investigate the effects of osteoporosis on the structural, densitometric and mechanical properties of the whole tibia using in vivo μCT imaging, spatiotemporal analysis and finite element modeling. Twelve C57Bl/6 female mice were adopted. At 14 weeks of age, half of the mice were ovariectomized (OVX), and the other half were SHAM-operated. The whole right tibia was scanned using an in vivo μCT imaging system at 14, 16, 17, 18, 19, 20, 21 and 22 weeks. The image datasets were registered in order to precisely quantify the bone properties. The results showed that OVX led to a significant increase in the endosteal area across the whole tibia 4 weeks after OVX intervention but did not have a significant influence on the periosteal area. Additionally, the bone volume and mineral content significantly decreased only in the proximal ...

Metal Finishing

Maxi-Blast Inc. will show the Pressure Dry Hone System designed for coating removal applications ... more Maxi-Blast Inc. will show the Pressure Dry Hone System designed for coating removal applications using plastic blast media. Samples of the environmentally safe plastic blast media will also be on display and available for hands-on demonstrations.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, Jan 16, 2016

The aim of this study is to explore a new method-high dose starting and low dose maintaining for ... more The aim of this study is to explore a new method-high dose starting and low dose maintaining for PEGylated Fibroblast growth factor 21 (pFGF-21) treatment. Db/db mice were initially treated with pFGF-21 of high dose, then treated with pFGF-21 of low doses. The mice were treated with pFGF-21 at initial dose of 1.0mg/kg for 14days, then treated with pFGF-21 at maintenance doses of 0.125/0.250/0.375/0.500mg/kg for 30days. The hypoglycemic and hypolipidemic effects of pFGF-21 of different maintenance doses were compared. The pharmacological efficacy of the maintenance doses of pFGF-21 was evaluated by blood glucose levels, oral glucose tolerance test, glycosylated hemoglobin levels, insulin levels, body weight, lipid profile parameters, the mRNA expressions of glycolysis-related genes, the mRNA expressions of gluconeogenesis-related genes and the mRNA expressions of lipid metabolism-related genes. Results showed that in comparison to the mice treated only with initial dose, the treatmen...

PloS one, 2016

Diabetes mellitus is a common endocrinopathy in dog. Fibroblast growth factor 21 (FGF-21) is a se... more Diabetes mellitus is a common endocrinopathy in dog. Fibroblast growth factor 21 (FGF-21) is a secreted protein, which is involved in glucose homeostasis. We speculate that the recombinant canine FGF-21 (cFGF-21) has the potential to become a powerful therapeutics to treat canine diabetes. The cFGF-21 gene was cloned and expressed in E. coli Rosetta (DE3). After purification, a cFGF-21 protein with the purity exceeding 95% was obtained. Mouse 3T3-L1 adipocytes and type 1 diabetic mice/dogs induced by STZ were used to examine the biological activity of cFGF-21 in vitro and in vivo, respectively. Results showed that cFGF-21 stimulated glucose uptake in adipocytes significantly in a dose-dependent manner, and reduced plasma glucose significantly in diabetic mice/dogs. After treatment with cFGF-21, the serum insulin level, glycosylated hemoglobin (HbA1c) level and the expressions of the hepatic gluconeogenesis genes (glucose-6-phosphatase, G6Pase and phosphoenolpyruvate carboxykinase, P...

2010 International Conference on Computational Intelligence and Software Engineering, 2010

... II. DESCRIPTION OF PSPM Based on public key system and digital signature technology, PSPM ...... more ... II. DESCRIPTION OF PSPM Based on public key system and digital signature technology, PSPM ... module, the unfamiliar node punishment mechanism module and channel encryption module. ... are claimed by the individual nodes, which is different from the usual MSA methods. ...

Journal of Organometallic Chemistry, 2014

ABSTRACT The equilibrium geometries, thermochemistry, and bonding of diiridium polynitrogen compo... more ABSTRACT The equilibrium geometries, thermochemistry, and bonding of diiridium polynitrogen compounds Ir-2(N-5)(4) were predicted using density functional theory. For Ir-2(N-5)(4) the preferred structure was a novel paddle wheel structure. Natural bonding orbital (NBO) analysis indicated that the bonding between the metal atom and the five-membered ring was predominantly ionic for Ir-2(N-5)(4) species. In addition, Nucleus independent chemical shift (NICS) values confirmed that the planar N-5(-) exhibited aromaticity in Ir-2(N-5)(4). The dissociation energies into mononuclear fragments for Ir-2(N-5)(4) were predicted to be 155.1 (149.7) kcal/mol, but Ir-2(N-5)(4) is thermodynamically unstable with respect to dissociation into Ir-2(N-5)(3) + N-5. Our most remarkable structural finding is the extremely short Iridium-Iridium distance (2.394 angstrom, MPW1PW91) predicted for the paddle wheel structure of Ir-2(N-5)(4). The new class of potential compounds was predicted stable enough for practical applications as high-energy density materials (HEDMs). (c) 2014 Elsevier B.V. All rights reserved.

Clinical biomechanics (Bristol, Avon), Jan 28, 2015

Symptomatic leg length inequality accounts for 8.7% of total hip replacement related claims made ... more Symptomatic leg length inequality accounts for 8.7% of total hip replacement related claims made against the UK National Health Service Litigation authority. It has not been established whether symptomatic leg length inequality patients following total hip replacement have abnormal hip kinetics during gait. Hip kinetics in 15 unilateral total hip replacement patients with symptomatic leg length inequality during gait was determined through multibody dynamics and compared to 15 native hip healthy controls and 15 'successful' asymptomatic unilateral total hip replacement patients. More significant differences from normal were found in symptomatic leg length inequality patients than in asymptomatic total hip replacement patients. The leg length inequality patients had altered functions defined by lower gait velocity, reduced stride length, reduced ground reaction force, decreased hip range of motion, reduced hip moment and less dynamic hip force with a 24% lower heel-strike pea...

Plant biotechnology journal, Jan 20, 2015

Elucidation of microRNA (miRNA) function through a loss-of-function approach has proven difficult... more Elucidation of microRNA (miRNA) function through a loss-of-function approach has proven difficult due to extensive genetic redundancy among most plant and animal miRNA families. Consequently, miRNA decoy technologies such as target MIMICs (MIMs) and short tandem target MIMICs (STTMs) in plants or molecular SPONGEs (SPs) in animals have been developed to generate loss-of-function phenotypes by perturbing endogenous miRNA activity. To test whether SPs can inhibit plant miRNA activity, synthetic SP transgenes containing multiple miRNA binding sites targeting different Arabidopsis miRNA families were generated. Additionally, their silencing efficacies were compared to the corresponding MIM and STTM transgenes via scoring the frequency and severity of phenotypic abnormalities elicited by each transgene. While SPs with wild-type miRNA binding sites have no apparent impact, SPs containing miRNA binding sites with two central mismatches (cmSPs) can generate strong loss-of-function phenotype...

2013 5th International Congress on Ultra Modern Telecommunications and Control Systems and Workshops (ICUMT), 2013

ABSTRACT The path geo-diversification mechanism introduced in this paper takes geographical diver... more ABSTRACT The path geo-diversification mechanism introduced in this paper takes geographical diversity of physical network topology into consideration when making routing decisions. It enables the network to be more resilient against area-based challenges by exploiting nodes' multiple ingress and egress ports. It shows better performance compared to OSPF when the network is subject to area-based challenges since the end nodes have access to multiple geographically diverse paths for their communication. We further incorporate geographical diversity into a new graph resilience metric cTGGD (compensated geographical graph diversity). This way we can effectively compare the resilience level of different topologies under regional-based network failures.

Medical engineering & physics, 2014

Hip hemiarthroplasty is a common treatment for femoral neck fracture. However, the acetabular car... more Hip hemiarthroplasty is a common treatment for femoral neck fracture. However, the acetabular cartilage may degenerate after hemiarthroplasty leading to postoperative failure and the need for revision surgery. The clearance between the acetabular cartilage and head of the prosthesis is one of the potential reasons for this failure. In this study, the influence of joint clearance on the biomechanical function of a generic hip model in hemiarthroplasty was investigated using biphasic numerical simulation. Both a prolonged loading period of 4000 s and dynamic gait load of 10 cycles were considered. It was found that a larger clearance led to a higher stress level, a faster reduction in load supported by the fluid and a faster cartilage consolidation process. Additionally, the mechanical performance of the acetabular cartilage in the natural model was similar to that in the hemiarthroplasty model with no clearance but different from the hemiarthroplasty models with clearances of 0.5mm a...

Clinical biomechanics (Bristol, Avon), 2014

Preclinical durability testing of hip replacement implants is standardised by ISO-14242-1 (2002) ... more Preclinical durability testing of hip replacement implants is standardised by ISO-14242-1 (2002) which is based on historical inverse dynamics analysis using data obtained from a small sample of normal healthy individuals. It has not been established whether loading cycles derived from normal healthy individuals are representative of loading cycles occurring in patients following total hip replacement. Hip joint kinematics and hip contact forces derived from multibody modelling of forces during normal walking were obtained for 15 asymptomatic total hip replacement patients and compared to 38 normal healthy individuals and to the ISO standard for pre-clinical testing. Hip kinematics in the total hip replacement patients were comparable to the ISO data and the hip contact force in the normal healthy group was also comparable to the ISO cycles. Hip contact forces derived from the asymptomatic total hip replacement patients were comparable for the first part of the stance period but exh...

Journal of biomechanics, Jan 17, 2014

Edge loading can negatively impact the biomechanics and long-term performance of hip replacements... more Edge loading can negatively impact the biomechanics and long-term performance of hip replacements. Although edge loading has been widely investigated for hard-on-hard articulations, limited work has been conducted for hard-on-soft combinations. The aim of the present study was to investigate edge loading and its effect on the contact mechanics of a modular metal-on-polyethylene (MoP) total hip replacement (THR). A three-dimensional finite element model was developed based on a modular MoP bearing. Different cup inclination angles and head lateral microseparation were modelled and their effect on the contact mechanics of the modular MoP hip replacement were examined. The results showed that lateral microseparation caused loading of the head on the rim of the cup, which produced substantial increases in the maximum von Mises stress in the polyethylene liner and the maximum contact pressure on both the articulating surface and backside surface of the liner. Plastic deformation of the l...