Nitin kabra - Academia.edu (original) (raw)

Papers by Nitin kabra

PubMed, Feb 13, 2009

Aims/objectives: Over the last several decades, it has become amply evident that oxidative stress... more Aims/objectives: Over the last several decades, it has become amply evident that oxidative stress plays a significant role in the pathogenesis of coronary artery atherosclerosis and its complications. Relatively recent studies have demonstrated an association between increased oxidative stress and coronary artery disease. We therefore undertook this study to see the effect of free radical nitric oxide in patients with premature cardiovascular disease. Methods and results: The study included 50 individuals with premature coronary artery disease aged below 45 years, and 50 age- and sex-matched normal individuals for comparison. Free radical nitric oxide was estimated using the Griess reagent as previously described. Mean plasma nitric oxide was found to be high in the patient group as compared to the control group, which was found to be statistically significant at p > 0.0001. Conclusion: Increased NO production was present in the plasma of patients with premature coronary artery disease individuals as compared to normal controls. Since NO seems to have a role in tissue damage, high concentrations of NO could have probably given rise to the said clinical manifestations. Further studies are needed in this context as to whether the changed lifestyles in the young individuals or the type of occupation is leading to the oxidative stress and thereby to the disease.

PubMed, Sep 1, 2014

Aim: Detection of mutations in the genes for telomerase reverse transcriptase (TERT) in patients ... more Aim: Detection of mutations in the genes for telomerase reverse transcriptase (TERT) in patients of apparently acquired aplastic anaemia. Material and Methods: Five patients with apparently acquired aplastic anaemia and six unrelated healthy individuals were recruited for this study. The genomic DNA was extracted from whole blood of subjects (patients and controls) and amplified by Polymerase chain reaction. The amplified products were sequenced and analysed in an automated genetic-sequence analyser to identify mutations in genes encoding telomerase complex-namely DKC1, NOP10, NHP2, and TERT. Result: In this study, mutations were observed in both coding and non-coding regions of TERT gene. Out of five patients, four patients had novel nonsynonymous mutation in TERT. Another substitution mutation was found in DKC1 gene in a healthy control. There was an important observation that two healthy controls had mutations in the coding region of TERT and DKC1 genes, but no symptoms or haematological abnormalities were expressed in both controls. There was no significant difference observed (Z= 0.666; P= 0.506) between two groups (controls and patients) with respect to no. of individuals having mutations. Conclusion: The present study was undertaken to evaluate the mutation spectrum in the genes implicated in aplastic anaemia, i.e. TERT, DKC1, NOP10, and NHP2 in small case-control group (5+6). We have been successful in finding mutations in TERT and DKC1 while no population specific mutations were found in NOP10 and NHP2. The statistical significance of these mutations is difficult to establish as the sample size was too low. None of the patients with TERT mutations had a response to immunosuppressive therapy.

Journal of cardiovascular disease research, Jul 1, 2011

Background: Researchers have determined that Indians face a higher risk of heart disease, despite... more Background: Researchers have determined that Indians face a higher risk of heart disease, despite the fact that nearly half of them are vegetarians and lack many of the other traditional risk factors. In the below-30 age group, coronary artery disease mortality among Indians is threefold higher than in the whites in United Kingdom and tenfold higher than the Chinese in Singapore. High levels of homocysteine have been widely linked to the early onset of heart diseases in other populations, although a definite proof among Indians is lacking, which needs to be investigated by way of screening for factors responsible for high homocysteine levels. Objective: To screen for genetic factors responsible for hyperhomocysteinemia and the risk for premature coronary artery disease. Materials and Methods: A total of 100 individuals with proven premature coronary artery disease and 200 age-and-sex matched controls were screened for polymorphisms in Methylenetetrahydrofolate reductase (MTHFR) (C677T) Methionine synthase (MS) genes (A2756G, C2758G), and the B12 and Folate levels were estimated. Results: Results from the mutational analysis revealed that in the study group, seven individuals had a polymorphism for the C677T allele in the MTHFR gene (one homozygous and six heterozygous) (Fischer's Exact test P > 0.046) (OR: 0.2711 95% CI 0.0774 to 0.9491). Six were heterozygous for the A2756G polymorphism in the MS gene (Fischer's Exact test P > 0.0012). None showed a polymorphism at the C2758G allele in the MS gene. Four controls showed heterozygosity for the C677T polymorphism and none for the MS gene. The B12 and Folate levels were significantly lower in the study group as compared to the controls. Conclusions: It is important to know which factors determine the total homocysteine concentrations. In the general population, the most important modifiable determinants of tHcy are folate intake and coffee consumption. Smoking and alcohol consumption are also associated with the total homocysteine concentrations, but more research is necessary to elucidate whether these relations are not originating from residual confounding due to other lifestyle factors.

Indian heart journal

Over the last several decades, it has become amply evident that oxidative stress plays a signific... more Over the last several decades, it has become amply evident that oxidative stress plays a significant role in the pathogenesis of coronary artery atherosclerosis and its complications. Relatively recent studies have demonstrated an association between increased oxidative stress and coronary artery disease. We therefore undertook this study to see the effect of free radical nitric oxide in patients with premature cardiovascular disease. The study included 50 individuals with premature coronary artery disease aged below 45 years, and 50 age- and sex-matched normal individuals for comparison. Free radical nitric oxide was estimated using the Griess reagent as previously described. Mean plasma nitric oxide was found to be high in the patient group as compared to the control group, which was found to be statistically significant at p > 0.0001. Increased NO production was present in the plasma of patients with premature coronary artery disease individuals as compared to normal controls....

International Journal of Cardiology, 2022

Indian Journal of Cardiovascular Disease in Women - WINCARS, 2021

Background: Prosthetic valve thrombosis (PVT) is a rare but serious complication of valve replace... more Background: Prosthetic valve thrombosis (PVT) is a rare but serious complication of valve replacement, most often encountered with mechanical prostheses. The significant morbidity and mortality associated with this condition warrants rapid diagnostic evaluation. Transthoracic, TEE and Cinefluoroscopy represent the main diagnostic procedures. Methods: The present study is an institutional based single centre prospective and retrospective observational study conducted in patients who are admitted with diagnosis of prosthetic valve thrombosis to the Department Of Cardiology, Gandhi Medical college/Hospital, Secunderabad, Telangana, India. The study was conducted between April 2016 to February 2018. A total of 66 patients admitted to the department of cardiology with a diagnosis of prosthetic valve thrombosis. Results: A total of 66 patients included in this study had mean age of 35.2 ±10.5 years and 43(65.15%) are females and 23(34.85%) are males. 58(87.88%) patients underwent MVR, 4(6...

The Journal of the Association of Physicians of India, 2014

Journal of …, 2011