kadriye demirkaya - Academia.edu (original) (raw)

Papers by kadriye demirkaya

Research paper thumbnail of Cyclic fatigue resistance of new and used Mtwo rotary nickel‐titanium instruments in two different radii of curvature

Australian Endodontic Journal, 2010

The fracture of nickel‐titanium rotary instruments is a serious problem that can jeopardise the o... more The fracture of nickel‐titanium rotary instruments is a serious problem that can jeopardise the outcome of root canal treatment. The aim of this study was to compare the cyclic fatigue resistance of new and used Mtwo rotary nickel‐titanium instruments in artificial canals with two different radii of curvature. Four hundred and twenty Mtwo instruments were used in the present study. Thirty new and 30 used instruments of each size were tested for cyclic fatigue in artificial canals with radii of curvature of 5 or 10 mm. Each instrument was rotated until fracture occurred and the number of cycles to fracture was calculated by multiplying time to fracture and rotational speed. Data were analysed using a t‐test. According to the results of the study, radius of curvature was an important factor on cyclic fatigue resistance and clinical use significantly reduced the instrument lifespan.

Research paper thumbnail of Effect of Pretreatment State-Trait Anxiety Level of Endodontics Patients to Treatment Satisfaction

Gulhane Medical Journal, 2017

Demirkaya ve ark. Kadriye Demirkaya(*), Gökhan Şüyün(*) ÖZET Genel olarak anksiyete, korkunun; bi... more Demirkaya ve ark. Kadriye Demirkaya(*), Gökhan Şüyün(*) ÖZET Genel olarak anksiyete, korkunun; bilinmeyene karşı duyulan seklidir. Dental anksiyete, anksiyetenin sık karşılaşılan bir türü olup bireylerin hem ağız hem de genel sağlık üzerine olumsuz etkileri vardır. Çalışmamızda, daha önce hiç endodontik tedavi görmemiş hastaların tedavi öncesi durumluluk-süreklilik anksiyete düzeylerinin belirlemesi ve mevcut sosyo-demografik durumları ile tedavi sonrası memnuniyetlerinin karşılaştırılması amaçlanmıştır. Çalışmaya, kök-kanal tedavisi yapılması planlanan 69 erkek, 81 bayan toplam 150 hasta dahil edildi. Hastaların genel özellikleri (yaş, cinsiyet, eğitim düzeyi) kayıt altına alındıktan sonra, hastalara durumluluk ve süreklilik kaygı değerlerini ölçen Spielberger'in "State-Trait Anxiety Inventory (STAI) anket formları uygulandı. Ardından tüm hastaların standart kök kanal tedavileri yapıldı. Tedavi sonrasında hastaların tedaviden memnuniyetini ölçmeye yönelik olarak kullanılan Görsel Analog Hasta Tatmin Skalası (GAHTS) uygulandı. Durumsal olarak anksiyetik olan hastaların tedavi sonrası memnuniyeti üst seviyede tespit edildi. Yaş, cinsiyet ve eğitim durumları ile durumsal anksiyete, süreklilik anksiyetesi ve tedavi sonrası GAHTS puanı arasındaki ilişkiler, korelasyon katsayıları ve istatistiksel anlamlılıklar Spearman testi ile incelendi. Gruplar içindeki anlamlı farklar ise Kruskal Wallis testi, Mann-Whitney U testi kullanılarak araştırıldı. Yaş grupları arasında sadece, 45-60 yas grubu hastalar anlamlı olarak, 30-45 yas grubu hastalardan durumsal olarak anksiyetik olarak görüldü (p=0,007). Kadınlar, erkeklere kıyasla durumsal ve süreklilik anksiyetesi açısından anlamlı seviyede daha yüksek puanlar göstermiştir (p=0,001 ; p<0,001). Lise veya dengi seviyede okullardan mezun olanlar ise, üniversite mezunlarına göre durumsal olarak daha anksiyetik bulunmuştur (p<0,001). Endodontik tedaviler hastaların anksiyete düzeyini arttırmaktadır. Bu konuda kültürel anlamda uyumlanmış daha geniş ve detaylı araştırmalara ihtiyaç vardır.

Research paper thumbnail of Evaluation of micro surface structure and chemical composition of two different calcium silicate–containing filling materials

Turkish endodontic journal., 2018

A number of materials has been developed by various manufacturers for use as root-end filling mat... more A number of materials has been developed by various manufacturers for use as root-end filling materials. The first material to be developed specifically for this purpose was mineral trioxide aggregate (MTA). Although MTA is considered to have ideal properties, its usage remained limited due to its high-cost, difficult handling characteristics, long setting time, and the potential of discoloration. [1] These shortcomings of MTA led to continuous efforts in developing the modified versions of MTA. In 2001, MTA Angelus (MTA-A, Angelus Dental Solutions, Londrina, Brazil) was introduced as an alternative to ProRoot MTA, the first MTA product, and used in cer-Objective: To investigate and compare the composition and micro surface structure of two different calcium silicate-containing filling materials using energy dispersive X-ray spectroscopy (EDX) and scanning electron microscopy (SEM). Methods: The materials investigated included DiaRoot BioAggregate (BA) and MTA Angelus (MTA-A). After mixing, each filling material was placed into cubes of 3 mm3. The hardening samples were compressed and broken and these samples were used for SEM examination. For elemental analysis and chemical composition, some samples were powdered and EDX was performed. Results: EDX findings indicated that the major constituents of BA included calcium, oxygen, tantalum, and silicon. The chemical structure of MTA-A was similar to that of BA except for the absence of tantalum (radiopacifier). In addition, MTA-A contained some elements, e.g., aluminum, sodium, potassium, phosphorus, iron, rubidium, and strontium in trace amounts. The chemistry of compounds of BA filling material is more biologically compatible as a restorative material. In SEM images, BA was noted to be granular and almost spherical and particles of all sizes were observed. MTA-A was detected as a porous structure; its particles were granular, but locally planar layers were also detected. Conclusion: The mineralogical composition of BA was different from that of MTA-A. As opposed to MTA-A, BA did not contain tricalcium aluminate phase and it included tantalum oxide as a radiopacifier. SEM images of MTA-A represented a more porous surface structure than that of BA. In light of these findings, BioAggregate seems to be a more suitable root-end filling material in terms of mineral content and surface structure.

Research paper thumbnail of Efficacy of preheated chelating agents on calcium ion removal from instrumented root canals

Journal of Clinical and Experimental Dentistry, 2021

Background: The heating of chelating agents such as EDTA increases dentin wettability by decreasi... more Background: The heating of chelating agents such as EDTA increases dentin wettability by decreasing surface tension. However, the calcium ion release effect of preheated chelating agents in instrumented root canals has not yet been mentioned. In this study, it was aimed to evaluate the number of calcium ions removed by the pre-heated chelating agents from the root canals. Material and Methods: After 51 bovine teeth were instrumented, three of them were separated as negative controls and the remaining teeth were divided into six groups according to the temperature of the solution (at 22 or 37ºC): EDTA-22, CITRIC-22, QMix-22, EDTA-37, CITRIC-37 and QMix-37. Following irrigation, calcium ion levels were determined by atomic absorption spectrophotometer in chelating agents collected from the root canals. Results: QMix solution eliminated significantly more calcium ions than other chelating agents at different temperatures (p < 0.05). Regardless of the heating, QMix and 17% EDTA were significantly superior to 40% Citric acid (p < 0.05) while no significant difference was detected between QMix and 17% EDTA groups (p < 0.05). Heating all chelating agents did not significantly increase their ability to remove calcium ions from pre-instrumented root canals (P < 0.05). In the SEM examination, it was observed that the smear layer was removed from the middle third of the roots, except for the negative control group. Conclusions: Temperature changes have shown that these agents do not increase the ability of the smear layer to dissolve the inorganic structure. QMix at different temperatures may be recommended to use as the final chelating agent.

Research paper thumbnail of Natural irrigation solutions in endodontics

Gulhane Medical Journal, 2020

Recently, it has been discovered that it has a detrimental impact on dentin elasticity and bendin... more Recently, it has been discovered that it has a detrimental impact on dentin elasticity and bending resistance, as well (3,4). Another irrigation solution, chlorhexidine gluconate (CHX), is reported to show a bactericidal effect by causing coagulation of intracellular components at high concentrations, while it has shown a bacteriostatic effect at low concentrations (4,5).

Research paper thumbnail of Evaluation of micro surface structure and chemical composition of two different calcium silicate–containing filling materials

Turkish Endodontic Journal, 2018

A number of materials has been developed by various manufacturers for use as root-end filling mat... more A number of materials has been developed by various manufacturers for use as root-end filling materials. The first material to be developed specifically for this purpose was mineral trioxide aggregate (MTA). Although MTA is considered to have ideal properties, its usage remained limited due to its high-cost, difficult handling characteristics, long setting time, and the potential of discoloration. [1] These shortcomings of MTA led to continuous efforts in developing the modified versions of MTA. In 2001, MTA Angelus (MTA-A, Angelus Dental Solutions, Londrina, Brazil) was introduced as an alternative to ProRoot MTA, the first MTA product, and used in cer-Objective: To investigate and compare the composition and micro surface structure of two different calcium silicate-containing filling materials using energy dispersive X-ray spectroscopy (EDX) and scanning electron microscopy (SEM). Methods: The materials investigated included DiaRoot BioAggregate (BA) and MTA Angelus (MTA-A). After mixing, each filling material was placed into cubes of 3 mm3. The hardening samples were compressed and broken and these samples were used for SEM examination. For elemental analysis and chemical composition, some samples were powdered and EDX was performed. Results: EDX findings indicated that the major constituents of BA included calcium, oxygen, tantalum, and silicon. The chemical structure of MTA-A was similar to that of BA except for the absence of tantalum (radiopacifier). In addition, MTA-A contained some elements, e.g., aluminum, sodium, potassium, phosphorus, iron, rubidium, and strontium in trace amounts. The chemistry of compounds of BA filling material is more biologically compatible as a restorative material. In SEM images, BA was noted to be granular and almost spherical and particles of all sizes were observed. MTA-A was detected as a porous structure; its particles were granular, but locally planar layers were also detected. Conclusion: The mineralogical composition of BA was different from that of MTA-A. As opposed to MTA-A, BA did not contain tricalcium aluminate phase and it included tantalum oxide as a radiopacifier. SEM images of MTA-A represented a more porous surface structure than that of BA. In light of these findings, BioAggregate seems to be a more suitable root-end filling material in terms of mineral content and surface structure.

Research paper thumbnail of The effects of hydraulic calcium silicate containing endodontic materials on oxidative stress in erythrocytes and liver

Turkish Journal of Biochemistry, 2017

Objective The aim of this study was to evaluate the effects of hydraulic calcium silicate endodon... more Objective The aim of this study was to evaluate the effects of hydraulic calcium silicate endodontic cements, MTA Angelus, MTA Fillapex, and Theracal LC, on erythrocyte and liver oxidative stress parameters of rats. Methods Right upper incisor of each rat was extracted and polyethylene tubes containing the dental cements, or left empty for the control group, were inserted into the extraction socket. Blood and liver samples of each animal were obtained after 7, 30, or 60 days. Thiobarbituric acid reactive substances (TBARS) levels and catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities were determined by spectrophotometry. Results Erythrocyte and liver TBARS levels, and CAT and SOD enzymatic activities were significantly increased in dental cement applied groups compared with controls on day 7. The highest erythrocyte and liver TBARS concentrations were observed in the MTA Angelus group on day 7 of exposure. On day 30, erythrocyte CAT activity rema...

Research paper thumbnail of Gene expression changes in bioceramic paste-treated human dental pulp cells

Journal of Oral Science, 2016

We evaluated the gene expression profiles of human dental pulp cells exposed to iRoot BP using mi... more We evaluated the gene expression profiles of human dental pulp cells exposed to iRoot BP using microarray after 24 and 72 h. The results were verified using quantitative reverse transcriptase PCR analysis. Of the 36,000 transcripts arrayed, 21 were up-regulated and 15 were down-regulated by more than two fold. The largest group of up-regulated genes included those involved in nucleobase-containing compound metabolic processes, cell communication, protein metabolic processes, developmental processes, and biological regulation. The largest groups of down-regulated genes were those involved in cell communication, development, and biological regulation processes. In conclusion, iRoot BP affects the expression of genes involved in different biological processes in human dental pulp cells.

Research paper thumbnail of Selective 5-HT7 receptor agonists LP 44 and LP 211 elicit an analgesic effect on formalin-induced orofacial pain in mice

Journal of applied oral science : revista FOB

To investigate the antinociceptive effects of pharmacological activation of 5-HT7 receptors on or... more To investigate the antinociceptive effects of pharmacological activation of 5-HT7 receptors on orofacial pain in mice. Nociception was evaluated by using an orofacial formalin test in male Balb-C mice. Selective 5-HT7 receptor agonists, LP 44 and LP 211 (1, 5, and 10 mg/kg), were given intraperitoneally 30 min prior to a formalin injection. A bolus of 10 µl of 4% subcutaneous formalin was injected into the upper lip of mice and facial grooming behaviors were monitored. The behavioral responses consisted of two distinct periods, the early phase corresponding to acute pain (Phase I: 0-12 min) and the late phase (Phase II: 12-30 min). LP 44 and LP 211 (1, 5, and 10 mg/kg) produced an analgesic effect with reductions in face rubbing time in both Phase I and Phase II of the formalin test. Our results suggest that 5-HT7 receptor agonists may be promising analgesic drugs in the treatment of orofacial pain.

Research paper thumbnail of Effect of Pretreatment State-Trait Anxiety Level of Endodontics Patients to Treatment Satisfaction

Gulhane Medical Journal, 2017

Demirkaya ve ark. Kadriye Demirkaya(*), Gökhan Şüyün(*) ÖZET Genel olarak anksiyete, korkunun; bi... more Demirkaya ve ark. Kadriye Demirkaya(*), Gökhan Şüyün(*) ÖZET Genel olarak anksiyete, korkunun; bilinmeyene karşı duyulan seklidir. Dental anksiyete, anksiyetenin sık karşılaşılan bir türü olup bireylerin hem ağız hem de genel sağlık üzerine olumsuz etkileri vardır. Çalışmamızda, daha önce hiç endodontik tedavi görmemiş hastaların tedavi öncesi durumluluk-süreklilik anksiyete düzeylerinin belirlemesi ve mevcut sosyo-demografik durumları ile tedavi sonrası memnuniyetlerinin karşılaştırılması amaçlanmıştır. Çalışmaya, kök-kanal tedavisi yapılması planlanan 69 erkek, 81 bayan toplam 150 hasta dahil edildi. Hastaların genel özellikleri (yaş, cinsiyet, eğitim düzeyi) kayıt altına alındıktan sonra, hastalara durumluluk ve süreklilik kaygı değerlerini ölçen Spielberger'in "State-Trait Anxiety Inventory (STAI) anket formları uygulandı. Ardından tüm hastaların standart kök kanal tedavileri yapıldı. Tedavi sonrasında hastaların tedaviden memnuniyetini ölçmeye yönelik olarak kullanılan Görsel Analog Hasta Tatmin Skalası (GAHTS) uygulandı. Durumsal olarak anksiyetik olan hastaların tedavi sonrası memnuniyeti üst seviyede tespit edildi. Yaş, cinsiyet ve eğitim durumları ile durumsal anksiyete, süreklilik anksiyetesi ve tedavi sonrası GAHTS puanı arasındaki ilişkiler, korelasyon katsayıları ve istatistiksel anlamlılıklar Spearman testi ile incelendi. Gruplar içindeki anlamlı farklar ise Kruskal Wallis testi, Mann-Whitney U testi kullanılarak araştırıldı. Yaş grupları arasında sadece, 45-60 yas grubu hastalar anlamlı olarak, 30-45 yas grubu hastalardan durumsal olarak anksiyetik olarak görüldü (p=0,007). Kadınlar, erkeklere kıyasla durumsal ve süreklilik anksiyetesi açısından anlamlı seviyede daha yüksek puanlar göstermiştir (p=0,001 ; p<0,001). Lise veya dengi seviyede okullardan mezun olanlar ise, üniversite mezunlarına göre durumsal olarak daha anksiyetik bulunmuştur (p<0,001). Endodontik tedaviler hastaların anksiyete düzeyini arttırmaktadır. Bu konuda kültürel anlamda uyumlanmış daha geniş ve detaylı araştırmalara ihtiyaç vardır.

Research paper thumbnail of In vivo evaluation of the effects of hydraulic calcium silicate dental cements on plasma and liver aluminium levels in rats

European Journal of Oral Sciences, 2015

In vivo evaluation of the effects of hydraulic calcium silicate dental cements on plasma and live... more In vivo evaluation of the effects of hydraulic calcium silicate dental cements on plasma and liver aluminium levels in rats.

Research paper thumbnail of Effects of iRoot BP and white mineral trioxide aggregate on cell viability and the expression of genes associated with mineralization

International endodontic journal, Jan 7, 2014

To evaluate the cytotoxicity and mineralization effects of iRoot BP in human dental pulp cells (h... more To evaluate the cytotoxicity and mineralization effects of iRoot BP in human dental pulp cells (hDPCs) and to compare them with those of white mineral trioxide aggregate (WMTA). hDPCs were exposed to prepared dilutions (1 : 1-1 : 10) of the test materials. Cell viability was evaluated using the XTT assay after incubation periods of 24, 48 or 72 h. The expression of mineralization-related genes (bone morphogenic protein, osteonectin, bone sialoprotein, osteopontin, dentine sialophosphoprotein and collagen type 1) and heme oxygenase 1 was measured by quantitative real-time polymerase chain reaction (qRT-PCR) at 24 and 72 h. Statistical differences between test materials were analysed with the Mann-Whitney test. The 1 : 1 and 1 : 2 dilutions of iRoot BP were associated with higher cell viability after 24 h (P < 0.05). Only the 1 : 1 dilution of iRoot BP had higher cell viability after 48 h (P < 0.05), and there was no difference between iRoot BP and WMTA after 72 h (P > 0.05)....

Research paper thumbnail of Cross-sectional evaluation of the periapical status as related to quality of root canal fillings and coronal restorations in a rural adult male population of Turkey

BMC Oral Health, 2011

Background To determine the prevalence of periapical lesions in root canal-treated teeth in a rur... more Background To determine the prevalence of periapical lesions in root canal-treated teeth in a rural, male adult, Turkish population and to investigate the influence of the quality of root canal fillings on prevalence of periapical lesions. Methods The sample for this cross-sectional study consisted of 552 adult male patients, 18-32 years of age, presenting consecutively as new patients seeking routine dental care at the Dental Sciences of Gulhane Military Medicine, Ankara. The radiographs of the 1014 root canal-treated teeth were evaluated. The teeth were grouped according to the radiographic quality of the root canal filling and the coronal restoration. The criteria used for the examination were slightly modified from those described by De Moor. Periapical status was assessed by the Periapical Index scores (PAI) proposed by Orstavik. Results The overall success rate of root canal treatment was 32.1%. The success rates of adequately root canal treatment were significantly higher tha...

Research paper thumbnail of Hypoxia inhibits mineralization ability of human dental pulp cells treated with TEGDMA but increases cell survival in accordance with the culture time

Archives of Oral Biology, 2016

To evaluate the cytotoxicity and mineralization effects of TEGDMA in human dental pulp cells (hDP... more To evaluate the cytotoxicity and mineralization effects of TEGDMA in human dental pulp cells (hDPCs) under hypoxic and normoxic culture conditions. Design: Cell viability was evaluated using XTT assay after incubation periods of 24, 48, or 72 h. The expression of mineralization-related genes (osteonectin, osteopontin, dentin sialophosphoprotein, collagen type 1) and heme oxygenase 1 (HO-1) was assessed by quantitative real-time polymerase chain reaction at 24 and 72 h. Results: In XTT assay, viability was higher in 0.3, 1, 2, 4, and 5 mM groups in the presence of 21% O 2 after 24 h (p < 0.05). Additionally, while 0.3, 1, 2 mM groups had higher cell viability in the presence of 21% O 2 after 48 h (p < 0.05), in 3 mM groups cell viability was higher under 3% O 2 than 21% O 2 after both 24 and 48 h (p < 0.05). 1-3 mM groups had higher cell viability under 3% O 2 after 72 h (p < 0.05). There was no difference between 4 and 5 mM groups with regards to cell viability after 48 or 72 h (p > 0.05). In the gene expression study, TEGDMA-treated hDPCs showed lower mineralization potential in the presence of 3% than with 21% O 2 (p < 0.05). hDPCs revealed higher HO 1 expression in 0.3 and 1 mM groups under hypoxic than under normoxic conditions after a 72-h time period (p < 0.001). Conclusions: Hypoxic conditions increased cell survival in accordance with the culture period but inhibited the odontoblastic differentiation of hDPCs treated with TEGDMA.

Research paper thumbnail of Cyclic fatigue resistance of new and used Mtwo rotary nickel‐titanium instruments in two different radii of curvature

Australian Endodontic Journal, 2010

The fracture of nickel‐titanium rotary instruments is a serious problem that can jeopardise the o... more The fracture of nickel‐titanium rotary instruments is a serious problem that can jeopardise the outcome of root canal treatment. The aim of this study was to compare the cyclic fatigue resistance of new and used Mtwo rotary nickel‐titanium instruments in artificial canals with two different radii of curvature. Four hundred and twenty Mtwo instruments were used in the present study. Thirty new and 30 used instruments of each size were tested for cyclic fatigue in artificial canals with radii of curvature of 5 or 10 mm. Each instrument was rotated until fracture occurred and the number of cycles to fracture was calculated by multiplying time to fracture and rotational speed. Data were analysed using a t‐test. According to the results of the study, radius of curvature was an important factor on cyclic fatigue resistance and clinical use significantly reduced the instrument lifespan.

Research paper thumbnail of Effect of Pretreatment State-Trait Anxiety Level of Endodontics Patients to Treatment Satisfaction

Gulhane Medical Journal, 2017

Demirkaya ve ark. Kadriye Demirkaya(*), Gökhan Şüyün(*) ÖZET Genel olarak anksiyete, korkunun; bi... more Demirkaya ve ark. Kadriye Demirkaya(*), Gökhan Şüyün(*) ÖZET Genel olarak anksiyete, korkunun; bilinmeyene karşı duyulan seklidir. Dental anksiyete, anksiyetenin sık karşılaşılan bir türü olup bireylerin hem ağız hem de genel sağlık üzerine olumsuz etkileri vardır. Çalışmamızda, daha önce hiç endodontik tedavi görmemiş hastaların tedavi öncesi durumluluk-süreklilik anksiyete düzeylerinin belirlemesi ve mevcut sosyo-demografik durumları ile tedavi sonrası memnuniyetlerinin karşılaştırılması amaçlanmıştır. Çalışmaya, kök-kanal tedavisi yapılması planlanan 69 erkek, 81 bayan toplam 150 hasta dahil edildi. Hastaların genel özellikleri (yaş, cinsiyet, eğitim düzeyi) kayıt altına alındıktan sonra, hastalara durumluluk ve süreklilik kaygı değerlerini ölçen Spielberger'in "State-Trait Anxiety Inventory (STAI) anket formları uygulandı. Ardından tüm hastaların standart kök kanal tedavileri yapıldı. Tedavi sonrasında hastaların tedaviden memnuniyetini ölçmeye yönelik olarak kullanılan Görsel Analog Hasta Tatmin Skalası (GAHTS) uygulandı. Durumsal olarak anksiyetik olan hastaların tedavi sonrası memnuniyeti üst seviyede tespit edildi. Yaş, cinsiyet ve eğitim durumları ile durumsal anksiyete, süreklilik anksiyetesi ve tedavi sonrası GAHTS puanı arasındaki ilişkiler, korelasyon katsayıları ve istatistiksel anlamlılıklar Spearman testi ile incelendi. Gruplar içindeki anlamlı farklar ise Kruskal Wallis testi, Mann-Whitney U testi kullanılarak araştırıldı. Yaş grupları arasında sadece, 45-60 yas grubu hastalar anlamlı olarak, 30-45 yas grubu hastalardan durumsal olarak anksiyetik olarak görüldü (p=0,007). Kadınlar, erkeklere kıyasla durumsal ve süreklilik anksiyetesi açısından anlamlı seviyede daha yüksek puanlar göstermiştir (p=0,001 ; p<0,001). Lise veya dengi seviyede okullardan mezun olanlar ise, üniversite mezunlarına göre durumsal olarak daha anksiyetik bulunmuştur (p<0,001). Endodontik tedaviler hastaların anksiyete düzeyini arttırmaktadır. Bu konuda kültürel anlamda uyumlanmış daha geniş ve detaylı araştırmalara ihtiyaç vardır.

Research paper thumbnail of Evaluation of micro surface structure and chemical composition of two different calcium silicate–containing filling materials

Turkish endodontic journal., 2018

A number of materials has been developed by various manufacturers for use as root-end filling mat... more A number of materials has been developed by various manufacturers for use as root-end filling materials. The first material to be developed specifically for this purpose was mineral trioxide aggregate (MTA). Although MTA is considered to have ideal properties, its usage remained limited due to its high-cost, difficult handling characteristics, long setting time, and the potential of discoloration. [1] These shortcomings of MTA led to continuous efforts in developing the modified versions of MTA. In 2001, MTA Angelus (MTA-A, Angelus Dental Solutions, Londrina, Brazil) was introduced as an alternative to ProRoot MTA, the first MTA product, and used in cer-Objective: To investigate and compare the composition and micro surface structure of two different calcium silicate-containing filling materials using energy dispersive X-ray spectroscopy (EDX) and scanning electron microscopy (SEM). Methods: The materials investigated included DiaRoot BioAggregate (BA) and MTA Angelus (MTA-A). After mixing, each filling material was placed into cubes of 3 mm3. The hardening samples were compressed and broken and these samples were used for SEM examination. For elemental analysis and chemical composition, some samples were powdered and EDX was performed. Results: EDX findings indicated that the major constituents of BA included calcium, oxygen, tantalum, and silicon. The chemical structure of MTA-A was similar to that of BA except for the absence of tantalum (radiopacifier). In addition, MTA-A contained some elements, e.g., aluminum, sodium, potassium, phosphorus, iron, rubidium, and strontium in trace amounts. The chemistry of compounds of BA filling material is more biologically compatible as a restorative material. In SEM images, BA was noted to be granular and almost spherical and particles of all sizes were observed. MTA-A was detected as a porous structure; its particles were granular, but locally planar layers were also detected. Conclusion: The mineralogical composition of BA was different from that of MTA-A. As opposed to MTA-A, BA did not contain tricalcium aluminate phase and it included tantalum oxide as a radiopacifier. SEM images of MTA-A represented a more porous surface structure than that of BA. In light of these findings, BioAggregate seems to be a more suitable root-end filling material in terms of mineral content and surface structure.

Research paper thumbnail of Efficacy of preheated chelating agents on calcium ion removal from instrumented root canals

Journal of Clinical and Experimental Dentistry, 2021

Background: The heating of chelating agents such as EDTA increases dentin wettability by decreasi... more Background: The heating of chelating agents such as EDTA increases dentin wettability by decreasing surface tension. However, the calcium ion release effect of preheated chelating agents in instrumented root canals has not yet been mentioned. In this study, it was aimed to evaluate the number of calcium ions removed by the pre-heated chelating agents from the root canals. Material and Methods: After 51 bovine teeth were instrumented, three of them were separated as negative controls and the remaining teeth were divided into six groups according to the temperature of the solution (at 22 or 37ºC): EDTA-22, CITRIC-22, QMix-22, EDTA-37, CITRIC-37 and QMix-37. Following irrigation, calcium ion levels were determined by atomic absorption spectrophotometer in chelating agents collected from the root canals. Results: QMix solution eliminated significantly more calcium ions than other chelating agents at different temperatures (p < 0.05). Regardless of the heating, QMix and 17% EDTA were significantly superior to 40% Citric acid (p < 0.05) while no significant difference was detected between QMix and 17% EDTA groups (p < 0.05). Heating all chelating agents did not significantly increase their ability to remove calcium ions from pre-instrumented root canals (P < 0.05). In the SEM examination, it was observed that the smear layer was removed from the middle third of the roots, except for the negative control group. Conclusions: Temperature changes have shown that these agents do not increase the ability of the smear layer to dissolve the inorganic structure. QMix at different temperatures may be recommended to use as the final chelating agent.

Research paper thumbnail of Natural irrigation solutions in endodontics

Gulhane Medical Journal, 2020

Recently, it has been discovered that it has a detrimental impact on dentin elasticity and bendin... more Recently, it has been discovered that it has a detrimental impact on dentin elasticity and bending resistance, as well (3,4). Another irrigation solution, chlorhexidine gluconate (CHX), is reported to show a bactericidal effect by causing coagulation of intracellular components at high concentrations, while it has shown a bacteriostatic effect at low concentrations (4,5).

Research paper thumbnail of Evaluation of micro surface structure and chemical composition of two different calcium silicate–containing filling materials

Turkish Endodontic Journal, 2018

A number of materials has been developed by various manufacturers for use as root-end filling mat... more A number of materials has been developed by various manufacturers for use as root-end filling materials. The first material to be developed specifically for this purpose was mineral trioxide aggregate (MTA). Although MTA is considered to have ideal properties, its usage remained limited due to its high-cost, difficult handling characteristics, long setting time, and the potential of discoloration. [1] These shortcomings of MTA led to continuous efforts in developing the modified versions of MTA. In 2001, MTA Angelus (MTA-A, Angelus Dental Solutions, Londrina, Brazil) was introduced as an alternative to ProRoot MTA, the first MTA product, and used in cer-Objective: To investigate and compare the composition and micro surface structure of two different calcium silicate-containing filling materials using energy dispersive X-ray spectroscopy (EDX) and scanning electron microscopy (SEM). Methods: The materials investigated included DiaRoot BioAggregate (BA) and MTA Angelus (MTA-A). After mixing, each filling material was placed into cubes of 3 mm3. The hardening samples were compressed and broken and these samples were used for SEM examination. For elemental analysis and chemical composition, some samples were powdered and EDX was performed. Results: EDX findings indicated that the major constituents of BA included calcium, oxygen, tantalum, and silicon. The chemical structure of MTA-A was similar to that of BA except for the absence of tantalum (radiopacifier). In addition, MTA-A contained some elements, e.g., aluminum, sodium, potassium, phosphorus, iron, rubidium, and strontium in trace amounts. The chemistry of compounds of BA filling material is more biologically compatible as a restorative material. In SEM images, BA was noted to be granular and almost spherical and particles of all sizes were observed. MTA-A was detected as a porous structure; its particles were granular, but locally planar layers were also detected. Conclusion: The mineralogical composition of BA was different from that of MTA-A. As opposed to MTA-A, BA did not contain tricalcium aluminate phase and it included tantalum oxide as a radiopacifier. SEM images of MTA-A represented a more porous surface structure than that of BA. In light of these findings, BioAggregate seems to be a more suitable root-end filling material in terms of mineral content and surface structure.

Research paper thumbnail of The effects of hydraulic calcium silicate containing endodontic materials on oxidative stress in erythrocytes and liver

Turkish Journal of Biochemistry, 2017

Objective The aim of this study was to evaluate the effects of hydraulic calcium silicate endodon... more Objective The aim of this study was to evaluate the effects of hydraulic calcium silicate endodontic cements, MTA Angelus, MTA Fillapex, and Theracal LC, on erythrocyte and liver oxidative stress parameters of rats. Methods Right upper incisor of each rat was extracted and polyethylene tubes containing the dental cements, or left empty for the control group, were inserted into the extraction socket. Blood and liver samples of each animal were obtained after 7, 30, or 60 days. Thiobarbituric acid reactive substances (TBARS) levels and catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities were determined by spectrophotometry. Results Erythrocyte and liver TBARS levels, and CAT and SOD enzymatic activities were significantly increased in dental cement applied groups compared with controls on day 7. The highest erythrocyte and liver TBARS concentrations were observed in the MTA Angelus group on day 7 of exposure. On day 30, erythrocyte CAT activity rema...

Research paper thumbnail of Gene expression changes in bioceramic paste-treated human dental pulp cells

Journal of Oral Science, 2016

We evaluated the gene expression profiles of human dental pulp cells exposed to iRoot BP using mi... more We evaluated the gene expression profiles of human dental pulp cells exposed to iRoot BP using microarray after 24 and 72 h. The results were verified using quantitative reverse transcriptase PCR analysis. Of the 36,000 transcripts arrayed, 21 were up-regulated and 15 were down-regulated by more than two fold. The largest group of up-regulated genes included those involved in nucleobase-containing compound metabolic processes, cell communication, protein metabolic processes, developmental processes, and biological regulation. The largest groups of down-regulated genes were those involved in cell communication, development, and biological regulation processes. In conclusion, iRoot BP affects the expression of genes involved in different biological processes in human dental pulp cells.

Research paper thumbnail of Selective 5-HT7 receptor agonists LP 44 and LP 211 elicit an analgesic effect on formalin-induced orofacial pain in mice

Journal of applied oral science : revista FOB

To investigate the antinociceptive effects of pharmacological activation of 5-HT7 receptors on or... more To investigate the antinociceptive effects of pharmacological activation of 5-HT7 receptors on orofacial pain in mice. Nociception was evaluated by using an orofacial formalin test in male Balb-C mice. Selective 5-HT7 receptor agonists, LP 44 and LP 211 (1, 5, and 10 mg/kg), were given intraperitoneally 30 min prior to a formalin injection. A bolus of 10 µl of 4% subcutaneous formalin was injected into the upper lip of mice and facial grooming behaviors were monitored. The behavioral responses consisted of two distinct periods, the early phase corresponding to acute pain (Phase I: 0-12 min) and the late phase (Phase II: 12-30 min). LP 44 and LP 211 (1, 5, and 10 mg/kg) produced an analgesic effect with reductions in face rubbing time in both Phase I and Phase II of the formalin test. Our results suggest that 5-HT7 receptor agonists may be promising analgesic drugs in the treatment of orofacial pain.

Research paper thumbnail of Effect of Pretreatment State-Trait Anxiety Level of Endodontics Patients to Treatment Satisfaction

Gulhane Medical Journal, 2017

Demirkaya ve ark. Kadriye Demirkaya(*), Gökhan Şüyün(*) ÖZET Genel olarak anksiyete, korkunun; bi... more Demirkaya ve ark. Kadriye Demirkaya(*), Gökhan Şüyün(*) ÖZET Genel olarak anksiyete, korkunun; bilinmeyene karşı duyulan seklidir. Dental anksiyete, anksiyetenin sık karşılaşılan bir türü olup bireylerin hem ağız hem de genel sağlık üzerine olumsuz etkileri vardır. Çalışmamızda, daha önce hiç endodontik tedavi görmemiş hastaların tedavi öncesi durumluluk-süreklilik anksiyete düzeylerinin belirlemesi ve mevcut sosyo-demografik durumları ile tedavi sonrası memnuniyetlerinin karşılaştırılması amaçlanmıştır. Çalışmaya, kök-kanal tedavisi yapılması planlanan 69 erkek, 81 bayan toplam 150 hasta dahil edildi. Hastaların genel özellikleri (yaş, cinsiyet, eğitim düzeyi) kayıt altına alındıktan sonra, hastalara durumluluk ve süreklilik kaygı değerlerini ölçen Spielberger'in "State-Trait Anxiety Inventory (STAI) anket formları uygulandı. Ardından tüm hastaların standart kök kanal tedavileri yapıldı. Tedavi sonrasında hastaların tedaviden memnuniyetini ölçmeye yönelik olarak kullanılan Görsel Analog Hasta Tatmin Skalası (GAHTS) uygulandı. Durumsal olarak anksiyetik olan hastaların tedavi sonrası memnuniyeti üst seviyede tespit edildi. Yaş, cinsiyet ve eğitim durumları ile durumsal anksiyete, süreklilik anksiyetesi ve tedavi sonrası GAHTS puanı arasındaki ilişkiler, korelasyon katsayıları ve istatistiksel anlamlılıklar Spearman testi ile incelendi. Gruplar içindeki anlamlı farklar ise Kruskal Wallis testi, Mann-Whitney U testi kullanılarak araştırıldı. Yaş grupları arasında sadece, 45-60 yas grubu hastalar anlamlı olarak, 30-45 yas grubu hastalardan durumsal olarak anksiyetik olarak görüldü (p=0,007). Kadınlar, erkeklere kıyasla durumsal ve süreklilik anksiyetesi açısından anlamlı seviyede daha yüksek puanlar göstermiştir (p=0,001 ; p<0,001). Lise veya dengi seviyede okullardan mezun olanlar ise, üniversite mezunlarına göre durumsal olarak daha anksiyetik bulunmuştur (p<0,001). Endodontik tedaviler hastaların anksiyete düzeyini arttırmaktadır. Bu konuda kültürel anlamda uyumlanmış daha geniş ve detaylı araştırmalara ihtiyaç vardır.

Research paper thumbnail of In vivo evaluation of the effects of hydraulic calcium silicate dental cements on plasma and liver aluminium levels in rats

European Journal of Oral Sciences, 2015

In vivo evaluation of the effects of hydraulic calcium silicate dental cements on plasma and live... more In vivo evaluation of the effects of hydraulic calcium silicate dental cements on plasma and liver aluminium levels in rats.

Research paper thumbnail of Effects of iRoot BP and white mineral trioxide aggregate on cell viability and the expression of genes associated with mineralization

International endodontic journal, Jan 7, 2014

To evaluate the cytotoxicity and mineralization effects of iRoot BP in human dental pulp cells (h... more To evaluate the cytotoxicity and mineralization effects of iRoot BP in human dental pulp cells (hDPCs) and to compare them with those of white mineral trioxide aggregate (WMTA). hDPCs were exposed to prepared dilutions (1 : 1-1 : 10) of the test materials. Cell viability was evaluated using the XTT assay after incubation periods of 24, 48 or 72 h. The expression of mineralization-related genes (bone morphogenic protein, osteonectin, bone sialoprotein, osteopontin, dentine sialophosphoprotein and collagen type 1) and heme oxygenase 1 was measured by quantitative real-time polymerase chain reaction (qRT-PCR) at 24 and 72 h. Statistical differences between test materials were analysed with the Mann-Whitney test. The 1 : 1 and 1 : 2 dilutions of iRoot BP were associated with higher cell viability after 24 h (P < 0.05). Only the 1 : 1 dilution of iRoot BP had higher cell viability after 48 h (P < 0.05), and there was no difference between iRoot BP and WMTA after 72 h (P > 0.05)....

Research paper thumbnail of Cross-sectional evaluation of the periapical status as related to quality of root canal fillings and coronal restorations in a rural adult male population of Turkey

BMC Oral Health, 2011

Background To determine the prevalence of periapical lesions in root canal-treated teeth in a rur... more Background To determine the prevalence of periapical lesions in root canal-treated teeth in a rural, male adult, Turkish population and to investigate the influence of the quality of root canal fillings on prevalence of periapical lesions. Methods The sample for this cross-sectional study consisted of 552 adult male patients, 18-32 years of age, presenting consecutively as new patients seeking routine dental care at the Dental Sciences of Gulhane Military Medicine, Ankara. The radiographs of the 1014 root canal-treated teeth were evaluated. The teeth were grouped according to the radiographic quality of the root canal filling and the coronal restoration. The criteria used for the examination were slightly modified from those described by De Moor. Periapical status was assessed by the Periapical Index scores (PAI) proposed by Orstavik. Results The overall success rate of root canal treatment was 32.1%. The success rates of adequately root canal treatment were significantly higher tha...

Research paper thumbnail of Hypoxia inhibits mineralization ability of human dental pulp cells treated with TEGDMA but increases cell survival in accordance with the culture time

Archives of Oral Biology, 2016

To evaluate the cytotoxicity and mineralization effects of TEGDMA in human dental pulp cells (hDP... more To evaluate the cytotoxicity and mineralization effects of TEGDMA in human dental pulp cells (hDPCs) under hypoxic and normoxic culture conditions. Design: Cell viability was evaluated using XTT assay after incubation periods of 24, 48, or 72 h. The expression of mineralization-related genes (osteonectin, osteopontin, dentin sialophosphoprotein, collagen type 1) and heme oxygenase 1 (HO-1) was assessed by quantitative real-time polymerase chain reaction at 24 and 72 h. Results: In XTT assay, viability was higher in 0.3, 1, 2, 4, and 5 mM groups in the presence of 21% O 2 after 24 h (p < 0.05). Additionally, while 0.3, 1, 2 mM groups had higher cell viability in the presence of 21% O 2 after 48 h (p < 0.05), in 3 mM groups cell viability was higher under 3% O 2 than 21% O 2 after both 24 and 48 h (p < 0.05). 1-3 mM groups had higher cell viability under 3% O 2 after 72 h (p < 0.05). There was no difference between 4 and 5 mM groups with regards to cell viability after 48 or 72 h (p > 0.05). In the gene expression study, TEGDMA-treated hDPCs showed lower mineralization potential in the presence of 3% than with 21% O 2 (p < 0.05). hDPCs revealed higher HO 1 expression in 0.3 and 1 mM groups under hypoxic than under normoxic conditions after a 72-h time period (p < 0.001). Conclusions: Hypoxic conditions increased cell survival in accordance with the culture period but inhibited the odontoblastic differentiation of hDPCs treated with TEGDMA.