Kanwal Asif - Academia.edu (original) (raw)
Papers by Kanwal Asif
Journal of Materials Chemistry B
A schematic representation of FeNP-induced ROS production and its involvement in ferroptosis.
Journal of Advanced Research
Sensors and Actuators B: Chemical, 2022
This paper reports on an ultrasensitive and label-free electrochemical immunosensor for monitorin... more This paper reports on an ultrasensitive and label-free electrochemical immunosensor for monitoring the SARS-CoV-2 spike protein (SARS-CoV-2 SP). A self-supported electrode, which can simultaneously serve as an antibody immobilization matrix and electron transport channel, was initially fabricated by a controlled partial exfoliation of a flexible graphitic carbon foil (GCF). Mild acidic treatment enabled the partial oxidation and exfoliation (down to a few layers) of the flexible GCF; this also provided a high percentage of oxygen functionality and an enhanced surface roughness. The substrate electrode was further functionalized with ethylenediamine (EDA) to provide a suitable platform with even a higher surface roughness, for the covalent immobilization of an anti-SARS-CoV-2 antibody. The change in the current response for the [Fe(CN)6]3−/4− redox couple, induced by the binding of SARS-CoV-2 SP to the antibody immobilized on the electrode surface, was used to determine the SARS-CoV-2 SP concentration. The immunosensor thus prepared could detect SARS-CoV-2 SP within 30 min with high reproducibility and specificity over a wide concentration range (0.2–100 ng/mL). Detection limits of 25 pg/mL and 27 pg/mL were found in a phosphate buffer solution (pH 7.4), and diluted blood plasma, respectively. The immunosensor was also employed to detect SARS-CoV-2 SP in artificial human saliva.
Advanced Functional Materials, 2021
Assessment of glucose concentration is important in the diagnosis and treatment of diabetes. Sinc... more Assessment of glucose concentration is important in the diagnosis and treatment of diabetes. Since the introduction of enzymatic glucose biosensors, scientific and technological advances in nanomaterials have led to the development of new generations of glucose sensors. This field has witnessed major developments over the last decade, as the novel nanomaterials are capable of efficiently catalyzing glucose directly (i.e., act as artificial enzymes, therefore defined nanozymes) or to entrap enzymes that are able to oxidize glucose. Among other nanomaterials, metal–organic frameworks (MOFs) have recently provided a tremendous basis to construct glucose sensing devices. MOFs are large porous crystalline compounds with versatile structural and tuneable chemical properties. In addition, they possess catalytic, peroxidase‐like, and electrochemical redox activity. This review comprehensively summarizes the general characteristics of MOFs, their subtypes, and MOF composites, as well as MOF‐derived materials employed to construct electrochemical, optical, transistor, and microfluidic devices for the detection of glucose. They include enzymatic, nonenzymatic, wearable, and flexible sensing devices and methods. The review also outlines the design and synthesis of MOFs and the working principles of the different transduction‐based glucose sensors and highlights the current challenges and future perspectives.
Biosensors and Bioelectronics: X
Cancers, 2021
Cancer is one of the major causes of death in developed countries and current therapies are based... more Cancer is one of the major causes of death in developed countries and current therapies are based on surgery, chemotherapeutic agents, and radiation. To overcome side effects induced by chemo- and radiotherapy, in recent decades, targeted therapies have been proposed in second and even first lines. Targeted drugs act on the essential pathways involved in tumor induction, progression, and metastasis, basically all the hallmark of cancers. Among emerging pathways, the cholesterol metabolic pathway is a strong candidate for this purpose. Cancer cells have an accelerated metabolic rate and require a continuous supply of cholesterol for cell division and membrane renewal. Steroidogenic acute regulatory related lipid transfer (START) proteins are a family of proteins involved in the transfer of lipids and some of them are important in non-vesicular cholesterol transportation within the cell. The alteration of their expression levels is implicated in several diseases, including cancers. In...
Journal of Materials Chemistry B
A schematic representation of FeNP-induced ROS production and its involvement in ferroptosis.
Journal of Advanced Research
Sensors and Actuators B: Chemical, 2022
This paper reports on an ultrasensitive and label-free electrochemical immunosensor for monitorin... more This paper reports on an ultrasensitive and label-free electrochemical immunosensor for monitoring the SARS-CoV-2 spike protein (SARS-CoV-2 SP). A self-supported electrode, which can simultaneously serve as an antibody immobilization matrix and electron transport channel, was initially fabricated by a controlled partial exfoliation of a flexible graphitic carbon foil (GCF). Mild acidic treatment enabled the partial oxidation and exfoliation (down to a few layers) of the flexible GCF; this also provided a high percentage of oxygen functionality and an enhanced surface roughness. The substrate electrode was further functionalized with ethylenediamine (EDA) to provide a suitable platform with even a higher surface roughness, for the covalent immobilization of an anti-SARS-CoV-2 antibody. The change in the current response for the [Fe(CN)6]3−/4− redox couple, induced by the binding of SARS-CoV-2 SP to the antibody immobilized on the electrode surface, was used to determine the SARS-CoV-2 SP concentration. The immunosensor thus prepared could detect SARS-CoV-2 SP within 30 min with high reproducibility and specificity over a wide concentration range (0.2–100 ng/mL). Detection limits of 25 pg/mL and 27 pg/mL were found in a phosphate buffer solution (pH 7.4), and diluted blood plasma, respectively. The immunosensor was also employed to detect SARS-CoV-2 SP in artificial human saliva.
Advanced Functional Materials, 2021
Assessment of glucose concentration is important in the diagnosis and treatment of diabetes. Sinc... more Assessment of glucose concentration is important in the diagnosis and treatment of diabetes. Since the introduction of enzymatic glucose biosensors, scientific and technological advances in nanomaterials have led to the development of new generations of glucose sensors. This field has witnessed major developments over the last decade, as the novel nanomaterials are capable of efficiently catalyzing glucose directly (i.e., act as artificial enzymes, therefore defined nanozymes) or to entrap enzymes that are able to oxidize glucose. Among other nanomaterials, metal–organic frameworks (MOFs) have recently provided a tremendous basis to construct glucose sensing devices. MOFs are large porous crystalline compounds with versatile structural and tuneable chemical properties. In addition, they possess catalytic, peroxidase‐like, and electrochemical redox activity. This review comprehensively summarizes the general characteristics of MOFs, their subtypes, and MOF composites, as well as MOF‐derived materials employed to construct electrochemical, optical, transistor, and microfluidic devices for the detection of glucose. They include enzymatic, nonenzymatic, wearable, and flexible sensing devices and methods. The review also outlines the design and synthesis of MOFs and the working principles of the different transduction‐based glucose sensors and highlights the current challenges and future perspectives.
Biosensors and Bioelectronics: X
Cancers, 2021
Cancer is one of the major causes of death in developed countries and current therapies are based... more Cancer is one of the major causes of death in developed countries and current therapies are based on surgery, chemotherapeutic agents, and radiation. To overcome side effects induced by chemo- and radiotherapy, in recent decades, targeted therapies have been proposed in second and even first lines. Targeted drugs act on the essential pathways involved in tumor induction, progression, and metastasis, basically all the hallmark of cancers. Among emerging pathways, the cholesterol metabolic pathway is a strong candidate for this purpose. Cancer cells have an accelerated metabolic rate and require a continuous supply of cholesterol for cell division and membrane renewal. Steroidogenic acute regulatory related lipid transfer (START) proteins are a family of proteins involved in the transfer of lipids and some of them are important in non-vesicular cholesterol transportation within the cell. The alteration of their expression levels is implicated in several diseases, including cancers. In...