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Research paper thumbnail of Nanotechnology‐empowered therapeutics targeting neurodegenerative diseases

WIREs Nanomedicine and Nanobiotechnology

Neurodegenerative diseases are posing pressing health issues due to the high prevalence among agi... more Neurodegenerative diseases are posing pressing health issues due to the high prevalence among aging populations in the 21st century. They are evidenced by the progressive loss of neuronal function, often associated with neuronal necrosis and many related devastating complications. Nevertheless, effective therapeutical strategies to treat neurodegenerative diseases remain a tremendous challenge due to the multisystemic nature and limited drug delivery to the central nervous system. As a result, there is a pressing need to develop effective alternative therapeutics to manage the progression of neurodegenerative diseases. By utilizing the functional reconstructive materials and technologies with specific targeting ability at the nanoscale level, nanotechnology‐empowered medicines can transform the therapeutic paradigms of neurodegenerative diseases with minimal systemic side effects. This review outlines the current applications and progresses of the nanotechnology‐enabled drug deliver...

Research paper thumbnail of Immigration Picture Books by #ownvoices Authors

Georgia Journal of Literacy, 2020

Research paper thumbnail of Consistency of self-reported drug use events in a mixed methods study of people who inject drugs

The American Journal of Drug and Alcohol Abuse, 2015

Research paper thumbnail of Consenso de Rehabilitación Cardiovascular y Prevención Secundaria de las Sociedades Interamericana y Sudamericana de Cardiología

Consensus of cardiovascular rehabilitation and secondary prevention of American and South America... more Consensus of cardiovascular rehabilitation and secondary prevention of American and South American Societies of Cardiology

Research paper thumbnail of Aberrant promoter hypermethylation and genomic hypomethylation in tumor, adjacent normal tissues and blood from breast cancer patients

Anticancer research, 2010

Promoter hypermethylation and global hypomethylation in the human genome are hallmarks of most ca... more Promoter hypermethylation and global hypomethylation in the human genome are hallmarks of most cancers. Detection of aberrant methylation in white blood cells (WBC) has been suggested as a marker for cancer development, but has not been extensively investigated. This study was carried out to determine whether aberrant methylation in WBC DNA can be used as a surrogate biomarker for breast cancer risk. Promoter hypermethylation of 8 tumor suppressor genes (RASSF1A, APC, HIN1, BRCA1, CYCLIND2, RARbeta, CDH1 and TWIST1) and DNA methylation for three repetitive elements (LINE1, Sat2 and Alu) were analyzed in invasive ductal carcinoma of the breast, paired adjacent normal tissue and WBC from 40 breast cancer patients by the MethyLight assay. Methylation in WBC from 40 controls was also analyzed. Tumor and adjacent tissues showed frequent hypermethylation for all genes tested, while WBC DNA was rarely hypermethylated. For HIN1, RASSF1A, APC and TWIST1, there was agreement between hypermeth...

Research paper thumbnail of Correlations in global DNA methylation measures in peripheral blood mononuclear cells and granulocytes

Epigenetics : official journal of the DNA Methylation Society, Jan 2, 2014

Alterations in global DNA methylation levels have been associated with chronic diseases. Despite ... more Alterations in global DNA methylation levels have been associated with chronic diseases. Despite the increase in the number of studies measuring markers of global methylation, few have adequately examined within-individual differences by source of DNA and whether within-individual differences by source of DNA differ by age, race and other lifestyle factors. We examined correlations between peripheral mononuclear cell (PBMC) and granulocyte DNA methylation levels measured by the luminometric methylation assay (LUMA), and in LINE-1, Sat2, and Alu by MethyLight and pyrosequencing, in the same individual in 112 women participating in The New York City Multiethnic Breast Cancer Project. Levels of DNA methylation of Sat2 by MethyLight (r = 0.57; P < 0.01) and LINE-1 by pyrosequencing (r = 0.30; P < 0.01) were correlated between PBMC and granulocyte DNA of the same individuals, but LUMA and Alu levels were not. The magnitude of the correlations for Sat2 and LINE-1 varied when stratif...

Research paper thumbnail of Interannual Variability of the Meiobenthos and Hyperbenthos Communities from two Ecuadorian Sandy Beaches (1999-2001)

Investigaciones marinas, 2002

Research paper thumbnail of Características de las agresiones sexuales en beneficiarias del programa de tratamiento de depresión de un consultorio de atención primaria

Revista Chilena de Salud Pública, 2010

Research paper thumbnail of Availability and Characteristics of Cardiovascular Rehabilitation Programs in South America

Journal of Cardiopulmonary Rehabilitation and Prevention, 2013

Research paper thumbnail of Effect of α-tocopherol, α-tocotrienol and Rosa mosqueta shell extract on the performance of antioxidant-stripped canola oil (Brassica sp.) at high temperature

Research paper thumbnail of Early life socioeconomic factors and genomic DNA methylation in mid-life

Research paper thumbnail of Global DNA methylation levels in girls with and without a family history of breast cancer

Research paper thumbnail of Significant differences in global genomic DNA methylation by gender and race/ethnicity in peripheral blood

Research paper thumbnail of Physical activity and global genomic DNA methylation in a cancer-free population

Research paper thumbnail of DNA Methylation Changes Correlate with Gleason Score and Tumor Stage in Prostate Cancer

DNA and Cell Biology, 2012

Research paper thumbnail of Abstract 3772: Lifestyle factors and global methylation in peripheral leukocyte DNA

Cancer Research, 2010

Global hypomethylation is associated with genomic instability and may mediate the association bet... more Global hypomethylation is associated with genomic instability and may mediate the association between environmental and lifestyle factors and cancer risk. We examined the association between lifestyle factors and levels of genomic DNA methylation in the peripheral blood leukocytes of 160 participants in the North Texas Healthy Heart Study aged 45-75. We used in-person interviews for demographics and lifestyle factors, a self-administrated Block food frequency questionnaire for dietary data, bioelectrical impedance analysis (BIA) and CT-scan for body composition including fat mass % and areas of visceral and truncal subcutaneous adipose tissues at the L4L5 level. We measured genomic DNA methylation using bisulfite conversion of DNA and real time PCR (MethyLight) for SAT2-M1. The median level of global methylation was 61.3% in this cancer-free population. Male gender was positively associated with global hypomethylation (i.e. < median) (OR=3.7, 95%CI: 1.4-9.7). Compared to Hispanic...

Research paper thumbnail of Abstract 4936: Promoter methylation in prostate cancer: A pilot study

Cancer Research, 2010

Prostate cancer is a leading cause of cancer death among men in the United States. The current me... more Prostate cancer is a leading cause of cancer death among men in the United States. The current method used for the screening for prostate cancer, the prostate-specific antigen (PSA) test, has poor specificity and sensitivity. This has lead to the search for new biomarkers that can improve the prostate cancer screening, either by themselves or in combination with established markers. Epigenetic modifications, believed to occur early during tumorgenesis are one of the new measurements that could aid in improving prostate cancer screening. We conducted a case-control study to determine the association of promoter methylation of p16 INK4a and GSTP1 and prostate cancer risk. Twenty seven histologically confirmed prostate cancer patients were matched by age and ethnicity to twenty four controls. DNA was extracted from plasma, and percentage of DNA methylation was determined by pyrosequencing. We found that methylation levels were consistently higher in cases than in controls, but this dif...

Research paper thumbnail of Prenatal Tobacco Smoke Exposure and Genomewide Methylation in Adulthood

Cancer Epidemiology, Biomarkers & Prevention, 2011

Genomic DNA demethylation, including demethylation of repetitive elements (which comprise 45% of ... more Genomic DNA demethylation, including demethylation of repetitive elements (which comprise 45% of the human genome), has been linked to increased risk of breast and other cancers. Genomic DNA methylation can be altered prenatally and throughout life and may be a mechanism through which the environment alters disease risk. There is evidence that prenatal tobacco smoke exposure has a persistent impact on genomic DNA methylation; however, no study to date has assessed the association between prenatal smoke exposure and adult repetitive element methylation. We measured repetitive element methylation of Alu, LINE-1, and Sat2 using MethyLight in 92 members of the New York Women's Birth Cohort, a follow-up of former female participants of the New York site of the U.S. National Collaborative Perinatal Project (mean age at blood draw = 43.5, SD = 1.8). Prenatal smoke exposure was reported prospectively. We estimated associations using multivariable linear regression, and used the natural ...

Research paper thumbnail of Prenatal Smoke Exposure and Genomic DNA Methylation in a Multiethnic Birth Cohort

Cancer Epidemiology, Biomarkers & Prevention, 2011

Background: Exposure to prenatal tobacco smoke (PTS) has been associated with a number of health ... more Background: Exposure to prenatal tobacco smoke (PTS) has been associated with a number of health outcomes in the offspring, including some childhood cancers. Lower levels of genomic DNA methylation have also been associated with several types of cancers. We investigated whether PTS was associated with global DNA methylation levels in the offspring. Methods: Our sample was drawn from a birth cohort of women born between 1959 and 1963 in New York City (n = 90). We measured methylation of repetitive elements (Sat2, Alu, LINE-1) from peripheral blood granulocytes. We combined prospectively collected data on PTS with adult epidemiologic data and blood samples collected in 2001 to 2007 (mean age, 43 years). We used linear regression to assess the association between PTS and repetitive element methylation. Results: Thirty-six percent of mothers smoked during pregnancy. We observed an inverse association between PTS and Sat2 methylation. This inverse association remained even after adjustme...

Research paper thumbnail of Prognostic significance of gene-specific promoter hypermethylation in breast cancer patients

Breast Cancer Research and Treatment, 2011

Research paper thumbnail of Nanotechnology‐empowered therapeutics targeting neurodegenerative diseases

WIREs Nanomedicine and Nanobiotechnology

Neurodegenerative diseases are posing pressing health issues due to the high prevalence among agi... more Neurodegenerative diseases are posing pressing health issues due to the high prevalence among aging populations in the 21st century. They are evidenced by the progressive loss of neuronal function, often associated with neuronal necrosis and many related devastating complications. Nevertheless, effective therapeutical strategies to treat neurodegenerative diseases remain a tremendous challenge due to the multisystemic nature and limited drug delivery to the central nervous system. As a result, there is a pressing need to develop effective alternative therapeutics to manage the progression of neurodegenerative diseases. By utilizing the functional reconstructive materials and technologies with specific targeting ability at the nanoscale level, nanotechnology‐empowered medicines can transform the therapeutic paradigms of neurodegenerative diseases with minimal systemic side effects. This review outlines the current applications and progresses of the nanotechnology‐enabled drug deliver...

Research paper thumbnail of Immigration Picture Books by #ownvoices Authors

Georgia Journal of Literacy, 2020

Research paper thumbnail of Consistency of self-reported drug use events in a mixed methods study of people who inject drugs

The American Journal of Drug and Alcohol Abuse, 2015

Research paper thumbnail of Consenso de Rehabilitación Cardiovascular y Prevención Secundaria de las Sociedades Interamericana y Sudamericana de Cardiología

Consensus of cardiovascular rehabilitation and secondary prevention of American and South America... more Consensus of cardiovascular rehabilitation and secondary prevention of American and South American Societies of Cardiology

Research paper thumbnail of Aberrant promoter hypermethylation and genomic hypomethylation in tumor, adjacent normal tissues and blood from breast cancer patients

Anticancer research, 2010

Promoter hypermethylation and global hypomethylation in the human genome are hallmarks of most ca... more Promoter hypermethylation and global hypomethylation in the human genome are hallmarks of most cancers. Detection of aberrant methylation in white blood cells (WBC) has been suggested as a marker for cancer development, but has not been extensively investigated. This study was carried out to determine whether aberrant methylation in WBC DNA can be used as a surrogate biomarker for breast cancer risk. Promoter hypermethylation of 8 tumor suppressor genes (RASSF1A, APC, HIN1, BRCA1, CYCLIND2, RARbeta, CDH1 and TWIST1) and DNA methylation for three repetitive elements (LINE1, Sat2 and Alu) were analyzed in invasive ductal carcinoma of the breast, paired adjacent normal tissue and WBC from 40 breast cancer patients by the MethyLight assay. Methylation in WBC from 40 controls was also analyzed. Tumor and adjacent tissues showed frequent hypermethylation for all genes tested, while WBC DNA was rarely hypermethylated. For HIN1, RASSF1A, APC and TWIST1, there was agreement between hypermeth...

Research paper thumbnail of Correlations in global DNA methylation measures in peripheral blood mononuclear cells and granulocytes

Epigenetics : official journal of the DNA Methylation Society, Jan 2, 2014

Alterations in global DNA methylation levels have been associated with chronic diseases. Despite ... more Alterations in global DNA methylation levels have been associated with chronic diseases. Despite the increase in the number of studies measuring markers of global methylation, few have adequately examined within-individual differences by source of DNA and whether within-individual differences by source of DNA differ by age, race and other lifestyle factors. We examined correlations between peripheral mononuclear cell (PBMC) and granulocyte DNA methylation levels measured by the luminometric methylation assay (LUMA), and in LINE-1, Sat2, and Alu by MethyLight and pyrosequencing, in the same individual in 112 women participating in The New York City Multiethnic Breast Cancer Project. Levels of DNA methylation of Sat2 by MethyLight (r = 0.57; P < 0.01) and LINE-1 by pyrosequencing (r = 0.30; P < 0.01) were correlated between PBMC and granulocyte DNA of the same individuals, but LUMA and Alu levels were not. The magnitude of the correlations for Sat2 and LINE-1 varied when stratif...

Research paper thumbnail of Interannual Variability of the Meiobenthos and Hyperbenthos Communities from two Ecuadorian Sandy Beaches (1999-2001)

Investigaciones marinas, 2002

Research paper thumbnail of Características de las agresiones sexuales en beneficiarias del programa de tratamiento de depresión de un consultorio de atención primaria

Revista Chilena de Salud Pública, 2010

Research paper thumbnail of Availability and Characteristics of Cardiovascular Rehabilitation Programs in South America

Journal of Cardiopulmonary Rehabilitation and Prevention, 2013

Research paper thumbnail of Effect of α-tocopherol, α-tocotrienol and Rosa mosqueta shell extract on the performance of antioxidant-stripped canola oil (Brassica sp.) at high temperature

Research paper thumbnail of Early life socioeconomic factors and genomic DNA methylation in mid-life

Research paper thumbnail of Global DNA methylation levels in girls with and without a family history of breast cancer

Research paper thumbnail of Significant differences in global genomic DNA methylation by gender and race/ethnicity in peripheral blood

Research paper thumbnail of Physical activity and global genomic DNA methylation in a cancer-free population

Research paper thumbnail of DNA Methylation Changes Correlate with Gleason Score and Tumor Stage in Prostate Cancer

DNA and Cell Biology, 2012

Research paper thumbnail of Abstract 3772: Lifestyle factors and global methylation in peripheral leukocyte DNA

Cancer Research, 2010

Global hypomethylation is associated with genomic instability and may mediate the association bet... more Global hypomethylation is associated with genomic instability and may mediate the association between environmental and lifestyle factors and cancer risk. We examined the association between lifestyle factors and levels of genomic DNA methylation in the peripheral blood leukocytes of 160 participants in the North Texas Healthy Heart Study aged 45-75. We used in-person interviews for demographics and lifestyle factors, a self-administrated Block food frequency questionnaire for dietary data, bioelectrical impedance analysis (BIA) and CT-scan for body composition including fat mass % and areas of visceral and truncal subcutaneous adipose tissues at the L4L5 level. We measured genomic DNA methylation using bisulfite conversion of DNA and real time PCR (MethyLight) for SAT2-M1. The median level of global methylation was 61.3% in this cancer-free population. Male gender was positively associated with global hypomethylation (i.e. < median) (OR=3.7, 95%CI: 1.4-9.7). Compared to Hispanic...

Research paper thumbnail of Abstract 4936: Promoter methylation in prostate cancer: A pilot study

Cancer Research, 2010

Prostate cancer is a leading cause of cancer death among men in the United States. The current me... more Prostate cancer is a leading cause of cancer death among men in the United States. The current method used for the screening for prostate cancer, the prostate-specific antigen (PSA) test, has poor specificity and sensitivity. This has lead to the search for new biomarkers that can improve the prostate cancer screening, either by themselves or in combination with established markers. Epigenetic modifications, believed to occur early during tumorgenesis are one of the new measurements that could aid in improving prostate cancer screening. We conducted a case-control study to determine the association of promoter methylation of p16 INK4a and GSTP1 and prostate cancer risk. Twenty seven histologically confirmed prostate cancer patients were matched by age and ethnicity to twenty four controls. DNA was extracted from plasma, and percentage of DNA methylation was determined by pyrosequencing. We found that methylation levels were consistently higher in cases than in controls, but this dif...

Research paper thumbnail of Prenatal Tobacco Smoke Exposure and Genomewide Methylation in Adulthood

Cancer Epidemiology, Biomarkers & Prevention, 2011

Genomic DNA demethylation, including demethylation of repetitive elements (which comprise 45% of ... more Genomic DNA demethylation, including demethylation of repetitive elements (which comprise 45% of the human genome), has been linked to increased risk of breast and other cancers. Genomic DNA methylation can be altered prenatally and throughout life and may be a mechanism through which the environment alters disease risk. There is evidence that prenatal tobacco smoke exposure has a persistent impact on genomic DNA methylation; however, no study to date has assessed the association between prenatal smoke exposure and adult repetitive element methylation. We measured repetitive element methylation of Alu, LINE-1, and Sat2 using MethyLight in 92 members of the New York Women's Birth Cohort, a follow-up of former female participants of the New York site of the U.S. National Collaborative Perinatal Project (mean age at blood draw = 43.5, SD = 1.8). Prenatal smoke exposure was reported prospectively. We estimated associations using multivariable linear regression, and used the natural ...

Research paper thumbnail of Prenatal Smoke Exposure and Genomic DNA Methylation in a Multiethnic Birth Cohort

Cancer Epidemiology, Biomarkers & Prevention, 2011

Background: Exposure to prenatal tobacco smoke (PTS) has been associated with a number of health ... more Background: Exposure to prenatal tobacco smoke (PTS) has been associated with a number of health outcomes in the offspring, including some childhood cancers. Lower levels of genomic DNA methylation have also been associated with several types of cancers. We investigated whether PTS was associated with global DNA methylation levels in the offspring. Methods: Our sample was drawn from a birth cohort of women born between 1959 and 1963 in New York City (n = 90). We measured methylation of repetitive elements (Sat2, Alu, LINE-1) from peripheral blood granulocytes. We combined prospectively collected data on PTS with adult epidemiologic data and blood samples collected in 2001 to 2007 (mean age, 43 years). We used linear regression to assess the association between PTS and repetitive element methylation. Results: Thirty-six percent of mothers smoked during pregnancy. We observed an inverse association between PTS and Sat2 methylation. This inverse association remained even after adjustme...

Research paper thumbnail of Prognostic significance of gene-specific promoter hypermethylation in breast cancer patients

Breast Cancer Research and Treatment, 2011