katari Raju - Academia.edu (original) (raw)

Papers by katari Raju

Complementary DNA (cDNA)-oligodeoxythmidylate-celluloses were prepared from cDNA copies of polyso... more Complementary DNA (cDNA)-oligodeoxythmidylate-celluloses were prepared from cDNA copies of polysomal messenger RNA (mRNA) of Novikoff hepatoma, normal rat liver, and regenerating rat liver. cDNA synthesis with reverse transcriptase was approximately 46% with respect to input mRNA with oligodeoxythymidylate-cellulose primer. The cDNA's of normal liver, regenerating liver, and Novikoff hepatomas were used as affinity matrices for hybridization of different mRNA species. Under the conditions used, degradation of mRNA was not detected. After normalization for homologous hybridization efficiency, 53 and 65% of the Novikoff hepatoma mRNA bound to normal liver and regenerating liver cDNA's. Under these conditions an average of 82% of mRNA of normal liver bound to regenerating liver cDNA, and 92% of regenerating liver mRNA bound to normal liver cDNA. The bound and unbound mRNA's were analyzed by translation in the wheat germ system; 2-D gel analysis of the proteins synthesized in the wheat germ system indicated that the cDNA affinity columns selectively adsorbed some mRNA species.

PURPOSE: To obtain a molded composite polyester resin article improved in thermal shock resistanc... more PURPOSE: To obtain a molded composite polyester resin article improved in thermal shock resistance by molding a resin compsn. comprising an unsatd. polyester resin having a specified heat distortion temp. and an inorg. filler. CONSTITUTION: This molded article, having an improved thermal shock resistance to rupture of at least 600 cycles according to the more strict form of ANSI test Z 124,3, is prepd. by molding a composite compsn. comprising about 60-80 wt.% inorg. filler and about 20-40 wt.% unsatd. polyester. Such an improved thermal shock resistance is directly related to the use of an unsatd. polyester resin which in the unfilled state gives a cured item having a heat distortion temp. of about 68-78°C. Thus is obtd. a molded polyester article improved in thermal shock resistance. COPYRIGHT: (C)1995,JPO

JNCI: Journal of the National Cancer Institute, 1982

The ability to induce new formation of capillaries in the cornea was tested for ceruloplasmin, th... more The ability to induce new formation of capillaries in the cornea was tested for ceruloplasmin, the copper carrier of serum, for fragments of the ceruloplasmin molecule with and without copper, for heparin, and for glycyl-L-histidyl-L-lysine, bound or not bound to copper ions. Male or female 2- to 3-kg New Zealand White rabbits were used. These experiments were prompted by the previous observation of copper accumulation in the cornea during angiogenesis and by the inability of copper-deficient rabbits to mount an angiogenic response. The results showed that the three different molecules were all able to induce angiogenesis provided that they were bound to copper. Fragments of the ceruloplasmin molecule also induced angiogenesis but only when copper was bound to the peptides. The data are interpreted to indicate that copper ions are involved in the sequence of events leading to angiogenesis and that the carrier molecules may be of quite a different nature.

Cancer research, 1977

Complementary DNA (cDNA)-oligodeoxythmidylate-celluloses were prepared from cDNA copies of polyso... more Complementary DNA (cDNA)-oligodeoxythmidylate-celluloses were prepared from cDNA copies of polysomal messenger RNA (mRNA) of Novikoff hepatoma, normal rat liver, and regenerating rat liver. cDNA synthesis with reverse transcriptase was approximately 46% with respect to input mRNA with oligodeoxythymidylate-cellulose primer. The cDNA's of normal liver, regenerating liver, and Novikoff hepatomas were used as affinity matrices for hybridization of different mRNA species. Under the conditions used, degradation of mRNA was not detected. After normalization for homologous hybridization efficiency, 53 and 65% of the Novikoff hepatoma mRNA bound to normal liver and regenerating liver cDNA's. Under these conditions an average of 82% of mRNA of normal liver bound to regenerating liver cDNA, and 92% of regenerating liver mRNA bound to normal liver cDNA. The bound and unbound mRNA's were analyzed by translation in the wheat germ system; 2-D gel analysis of the proteins synthesized i...

Cancer research, 1978

Protein 64/7.2 (molecular weight/isoelectric point) is present in the cytosol of several hepatoma... more Protein 64/7.2 (molecular weight/isoelectric point) is present in the cytosol of several hepatomas including the Novikoff hepatoma and Morris hepatomas 9618A, 7794A, and 3924A, but it is not present in liver or 18-hr regenerating liver. Quantitatively, its concentration was highest in Novikoff hepatoma (150 microgram/g tissue) and Morris hepatoma 3924A (550 microgram/g tissue), which are rapid-growing tumors, less in Morris hepatoma 7794A (72 microgram/g tissue), which is of intermediate growth rate, and least in the slow-growing Morris hepatoma 9618A (25 microgram/g tissue). Protein 64/7.2 was isolated from Novikoff hepatoma ascites cells in high purity as shown by its migration as a single band on one-dimensional acid-urea-polyacrylamide gels and a single spot on two-dimensional isoelectric focusing sodium dodecyl sulfate-polyacrylamide gels. Its amino acid composition has an acidic to basic amino acid ratio of 1.6. Its amino-terminal amino acid is lysine, and its carboxyl-termina...

Cancer research, 1983

An assay to measure endothelial cell mobilization on a gelatin substratum has been developed. Uti... more An assay to measure endothelial cell mobilization on a gelatin substratum has been developed. Utilization of the gelatin-agarose and Boyden chamber assays established that: (a) fragments or extracts of corneas treated with several effectors of angiogenesis in vivo acquired the capacity to mobilize the capillary endothelium in vitro; (b) this mobilization was selective for the capillary endothelium; endothelium from aorta and fibroblasts from human skin or rabbit cornea were unresponsive; and (c) among the effectors of angiogenesis utilized alone; i.e., without the intermediary action of the cornea, none were able to mobilize the capillary endothelium in vitro, except for the heparin-copper complex. The data are interpreted to indicate that new formation of capillaries in vivo is the end result of a cascade of events of which heparin and copper are important components.

Cancer research, 1984

The mechanism of neovascularization was further explored by the use of chemically defined angioge... more The mechanism of neovascularization was further explored by the use of chemically defined angiogenesis effectors. The vascularization of the rabbit cornea was selected as an experimental approach that permits comparison of one cornea treated by the angiogenesis effector with the contralateral cornea of the same subject treated by the same molecule deprived of angiogenic capacity. Under these conditions, we observed that neovascularization was initiated by the appearance of a chemoattractant for the bovine capillary endothelium only in the cornea treated by the angiogenesis effector. The chemoattractant was purified about 150-fold by a single-step procedure, using gelatin:Sepharose affinity chromatography. Chemoattraction resulted from the combined effect of a chemotactic factor(s) and an activating factor(s). The association of the two enhanced 5- to 8-fold the motility of the capillary endothelium in a concentration-dependent manner with optimum at 0.2 mg/ml. The activating factor(...

Protein 45/7.8 (molecular weight x 10~3/isoelectric point) was found in the cytosol of several ra... more Protein 45/7.8 (molecular weight x 10~3/isoelectric point) was found in the cytosol of several rapidly growing hepatomas including Morris hepatomas 3924A and 9618A2 and Novikoff hepatoma. It was not found in Morris hepatomas 7794A and 8999, which have intermediate growth rates, or in the slow- growing Morris hepatoma 9618A, normal rat liver, 18-hr regen erating rat liver, or livers of fetal rats. Protein 45/7.8 was isolated under nondenaturing conditions from Morris hepato mas 3924A and 9618A2 and Novikoff hepatoma in high purity by purification in three steps: ammonium sulfate fractionation; diethylaminoethyl cellulose chromatography; and hydroxylap- atite chromatography. The protein was highly purified as shown by two-dimensional, isoelectric-focusing sodium dodecyl sul- fate:polyacrylamide gels. The amino acid composition of pro tein 45/7.8 from the three tumors studied was very similar; the acidic:basic amino acid ratio was 1.4. The NH2terminus amino acid of protein 45/7.8 was p...

Archives of Biochemistry and Biophysics, 1998

The yeastTrichosporon spec.SBUG 752 isolated from soil produced cytochrome P450 during the statio... more The yeastTrichosporon spec.SBUG 752 isolated from soil produced cytochrome P450 during the stationary phase of growth on glucose. After cell disruption and ultracentrifugation, large amounts of P450 (250 pmol/mg protein) were found in the cytosolic fraction. In contrast, no P450 was detectable in the microsomes. Similar results were also obtained from some other yeast species of the genusTrichosporon. After purification

Cancer Research, May 1, 1980

Complementary DNA (cDNA)-oligodeoxythmidylate-celluloses were prepared from cDNA copies of polyso... more Complementary DNA (cDNA)-oligodeoxythmidylate-celluloses were prepared from cDNA copies of polysomal messenger RNA (mRNA) of Novikoff hepatoma, normal rat liver, and regenerating rat liver. cDNA synthesis with reverse transcriptase was approximately 46% with respect to input mRNA with oligodeoxythymidylate-cellulose primer. The cDNA's of normal liver, regenerating liver, and Novikoff hepatomas were used as affinity matrices for hybridization of different mRNA species. Under the conditions used, degradation of mRNA was not detected. After normalization for homologous hybridization efficiency, 53 and 65% of the Novikoff hepatoma mRNA bound to normal liver and regenerating liver cDNA's. Under these conditions an average of 82% of mRNA of normal liver bound to regenerating liver cDNA, and 92% of regenerating liver mRNA bound to normal liver cDNA. The bound and unbound mRNA's were analyzed by translation in the wheat germ system; 2-D gel analysis of the proteins synthesized in the wheat germ system indicated that the cDNA affinity columns selectively adsorbed some mRNA species.

PURPOSE: To obtain a molded composite polyester resin article improved in thermal shock resistanc... more PURPOSE: To obtain a molded composite polyester resin article improved in thermal shock resistance by molding a resin compsn. comprising an unsatd. polyester resin having a specified heat distortion temp. and an inorg. filler. CONSTITUTION: This molded article, having an improved thermal shock resistance to rupture of at least 600 cycles according to the more strict form of ANSI test Z 124,3, is prepd. by molding a composite compsn. comprising about 60-80 wt.% inorg. filler and about 20-40 wt.% unsatd. polyester. Such an improved thermal shock resistance is directly related to the use of an unsatd. polyester resin which in the unfilled state gives a cured item having a heat distortion temp. of about 68-78°C. Thus is obtd. a molded polyester article improved in thermal shock resistance. COPYRIGHT: (C)1995,JPO

JNCI: Journal of the National Cancer Institute, 1982

The ability to induce new formation of capillaries in the cornea was tested for ceruloplasmin, th... more The ability to induce new formation of capillaries in the cornea was tested for ceruloplasmin, the copper carrier of serum, for fragments of the ceruloplasmin molecule with and without copper, for heparin, and for glycyl-L-histidyl-L-lysine, bound or not bound to copper ions. Male or female 2- to 3-kg New Zealand White rabbits were used. These experiments were prompted by the previous observation of copper accumulation in the cornea during angiogenesis and by the inability of copper-deficient rabbits to mount an angiogenic response. The results showed that the three different molecules were all able to induce angiogenesis provided that they were bound to copper. Fragments of the ceruloplasmin molecule also induced angiogenesis but only when copper was bound to the peptides. The data are interpreted to indicate that copper ions are involved in the sequence of events leading to angiogenesis and that the carrier molecules may be of quite a different nature.

Cancer research, 1977

Complementary DNA (cDNA)-oligodeoxythmidylate-celluloses were prepared from cDNA copies of polyso... more Complementary DNA (cDNA)-oligodeoxythmidylate-celluloses were prepared from cDNA copies of polysomal messenger RNA (mRNA) of Novikoff hepatoma, normal rat liver, and regenerating rat liver. cDNA synthesis with reverse transcriptase was approximately 46% with respect to input mRNA with oligodeoxythymidylate-cellulose primer. The cDNA's of normal liver, regenerating liver, and Novikoff hepatomas were used as affinity matrices for hybridization of different mRNA species. Under the conditions used, degradation of mRNA was not detected. After normalization for homologous hybridization efficiency, 53 and 65% of the Novikoff hepatoma mRNA bound to normal liver and regenerating liver cDNA's. Under these conditions an average of 82% of mRNA of normal liver bound to regenerating liver cDNA, and 92% of regenerating liver mRNA bound to normal liver cDNA. The bound and unbound mRNA's were analyzed by translation in the wheat germ system; 2-D gel analysis of the proteins synthesized i...

Cancer research, 1978

Protein 64/7.2 (molecular weight/isoelectric point) is present in the cytosol of several hepatoma... more Protein 64/7.2 (molecular weight/isoelectric point) is present in the cytosol of several hepatomas including the Novikoff hepatoma and Morris hepatomas 9618A, 7794A, and 3924A, but it is not present in liver or 18-hr regenerating liver. Quantitatively, its concentration was highest in Novikoff hepatoma (150 microgram/g tissue) and Morris hepatoma 3924A (550 microgram/g tissue), which are rapid-growing tumors, less in Morris hepatoma 7794A (72 microgram/g tissue), which is of intermediate growth rate, and least in the slow-growing Morris hepatoma 9618A (25 microgram/g tissue). Protein 64/7.2 was isolated from Novikoff hepatoma ascites cells in high purity as shown by its migration as a single band on one-dimensional acid-urea-polyacrylamide gels and a single spot on two-dimensional isoelectric focusing sodium dodecyl sulfate-polyacrylamide gels. Its amino acid composition has an acidic to basic amino acid ratio of 1.6. Its amino-terminal amino acid is lysine, and its carboxyl-termina...

Cancer research, 1983

An assay to measure endothelial cell mobilization on a gelatin substratum has been developed. Uti... more An assay to measure endothelial cell mobilization on a gelatin substratum has been developed. Utilization of the gelatin-agarose and Boyden chamber assays established that: (a) fragments or extracts of corneas treated with several effectors of angiogenesis in vivo acquired the capacity to mobilize the capillary endothelium in vitro; (b) this mobilization was selective for the capillary endothelium; endothelium from aorta and fibroblasts from human skin or rabbit cornea were unresponsive; and (c) among the effectors of angiogenesis utilized alone; i.e., without the intermediary action of the cornea, none were able to mobilize the capillary endothelium in vitro, except for the heparin-copper complex. The data are interpreted to indicate that new formation of capillaries in vivo is the end result of a cascade of events of which heparin and copper are important components.

Cancer research, 1984

The mechanism of neovascularization was further explored by the use of chemically defined angioge... more The mechanism of neovascularization was further explored by the use of chemically defined angiogenesis effectors. The vascularization of the rabbit cornea was selected as an experimental approach that permits comparison of one cornea treated by the angiogenesis effector with the contralateral cornea of the same subject treated by the same molecule deprived of angiogenic capacity. Under these conditions, we observed that neovascularization was initiated by the appearance of a chemoattractant for the bovine capillary endothelium only in the cornea treated by the angiogenesis effector. The chemoattractant was purified about 150-fold by a single-step procedure, using gelatin:Sepharose affinity chromatography. Chemoattraction resulted from the combined effect of a chemotactic factor(s) and an activating factor(s). The association of the two enhanced 5- to 8-fold the motility of the capillary endothelium in a concentration-dependent manner with optimum at 0.2 mg/ml. The activating factor(...

Protein 45/7.8 (molecular weight x 10~3/isoelectric point) was found in the cytosol of several ra... more Protein 45/7.8 (molecular weight x 10~3/isoelectric point) was found in the cytosol of several rapidly growing hepatomas including Morris hepatomas 3924A and 9618A2 and Novikoff hepatoma. It was not found in Morris hepatomas 7794A and 8999, which have intermediate growth rates, or in the slow- growing Morris hepatoma 9618A, normal rat liver, 18-hr regen erating rat liver, or livers of fetal rats. Protein 45/7.8 was isolated under nondenaturing conditions from Morris hepato mas 3924A and 9618A2 and Novikoff hepatoma in high purity by purification in three steps: ammonium sulfate fractionation; diethylaminoethyl cellulose chromatography; and hydroxylap- atite chromatography. The protein was highly purified as shown by two-dimensional, isoelectric-focusing sodium dodecyl sul- fate:polyacrylamide gels. The amino acid composition of pro tein 45/7.8 from the three tumors studied was very similar; the acidic:basic amino acid ratio was 1.4. The NH2terminus amino acid of protein 45/7.8 was p...

Archives of Biochemistry and Biophysics, 1998

The yeastTrichosporon spec.SBUG 752 isolated from soil produced cytochrome P450 during the statio... more The yeastTrichosporon spec.SBUG 752 isolated from soil produced cytochrome P450 during the stationary phase of growth on glucose. After cell disruption and ultracentrifugation, large amounts of P450 (250 pmol/mg protein) were found in the cytosolic fraction. In contrast, no P450 was detectable in the microsomes. Similar results were also obtained from some other yeast species of the genusTrichosporon. After purification

Cancer Research, May 1, 1980