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Papers by kaveh ahmadpanah

Journal of Aquaculture Development

Acta Biochimica et Biophysica Sinica, 2019

Chemosphere, 2017

Dimethoate is class I pesticide, highly toxic to aquatic organism. Ascorbic acid recuperate the f... more Dimethoate is class I pesticide, highly toxic to aquatic organism. Ascorbic acid recuperate the fish haematological and biochemical profiles. Ascorbic acid diet improve the erythrocytic indices in group C fish. Dietary ascorbic acid enhance the non-specific immune responses. Ascorbic acid withhold the toxicity exerted by dimethoate pesticide.

Ecotoxicology and environmental safety, Jan 15, 2016

Marine and Freshwater Behaviour and Physiology, 2019

This study investigated the effects of nonylphenol (NP) on hematological and immunological parame... more This study investigated the effects of nonylphenol (NP) on hematological and immunological parameters in both male and female rainbow trout (Oncorhynchus mykiss). Fish were randomly distributed into six groups and administered with NP (10, 50 and 100 μg g-1 week-1 BW) and a single dose of 17-β estradiol (E2; 2 μg g-1 week-1 BW, positive control). The solvent controls received ethanol and coconut oil as a vehicle, while the controls were not injected. Red blood cells (RBCs) count, hematocrit (Hct), hemoglobin (Hb), white blood cells (WBCs), and lymphocytes demonstrated a NP dose-dependent decrease, whereas mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), monocytes, and neutrophils showed an increasing trend in both male and female fish 21 days post-treatment. Also, RBCs, Hb, MCHC, WBCs, and lymphocytes were significantly reduced (p<0.05) in E2 treated fish. Lysozyme, complement components (C3 and C4) and immunoglobulin M (IgM) were increased in fish sera subjected to 10 and 50 μg g-1 NP, while these decreased in groups administered with 100 μg g-1 NP and 2 μg g-1 E2. Except for C4 level at 10 μg g-1 NP, no significant differences were observed in hematological and immunological parameters of male and female in each treatment. Overall, a frequent exposure to NP could lead to adverse effects on fish immunephysiological functions which may cause serious ecological threats of fish natural population sustainability.

This study investigated the effects of nonylphenol (NP) on hematological and immunological parame... more This study investigated the effects of nonylphenol (NP) on hematological and immunological parameters in both male and female rainbow trout (Oncorhynchus mykiss). Fish were randomly distributed into six groups and administered with NP (10, 50 and 100 μg g -1 week -1 BW) and a single dose of 17-β estradiol (E2; 2 μg g -1 week -1 BW, positive control). The solvent controls received ethanol and coconut oil as a vehicle, while the controls were not injected. Red blood cells (RBCs) count, hematocrit (Hct), hemoglobin (Hb), white blood cells (WBCs), and lymphocytes demonstrated a NP dose-dependent decrease, whereas mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), monocytes, and neutrophils showed an increasing trend in both male and female fish 21 days post-treatment. Also, RBCs, Hb, MCHC, WBCs, and lymphocytes were significantly reduced (p<0.05) in E2 treated fish. Lysozyme, complement components (C3 and C4) and immunoglobulin M (IgM) were increased in fish sera subjected to 10 and 50 μg g -1 NP, while these decreased in groups administered with 100 μg g -1 NP and 2 μg g -1 E2. Except for C4 level at 10 μg g -1 NP, no significant differences were observed in hematological and immunological parameters of male and female in each treatment. Overall, a frequent exposure to NP could lead to adverse effects on fish immunephysiological functions which may cause serious ecological threats of fish natural population sustainability.

Bisphenol A (BPA) is a well-known endocrine disrupting chemical (EDC), ubiquitous in the aquatic ... more Bisphenol A (BPA) is a well-known endocrine disrupting chemical (EDC), ubiquitous in the aquatic environment , which poses an ecotoxicological risk to the health of aquatic organisms. However, the im-munotoxic effects of its long-term exposure on fish have received limited attention. We examined a number of typical immune-related parameters and oxidative stress indices in the liver and blood serum of the red common carp (Cyprinus carpio), following a 30-day exposure to five different concentrations of BPA (0.1, 1, 10, 100, and 1000 μg/L). A significant increase in the hepato somatic index was observed in fish upon exposure to 1000 mg/L BPA, which correlated strongly with the accumulated BPA concentrations in fish bile. Induced oxidative stress was also apparent in the exposed fish liver, based on the enhanced levels of lipid peroxidation and inhibited activities of catalase, superoxide dismutase, and glutathione peroxidase. Serum lysozyme and C-reaction protein levels increased at low concentrations of exposure; however, they were significantly suppressed upon exposure to high concentrations. A significant increase was observed in the levels of immunoglobulin M, complement component 3, and alkaline phosphatase, in both fish liver and serum at low doses of 0.1 and 1 μg/L. This suggests that long-term exposure to environmentally relevant concentrations of BPA (even as low as 0.1 μg/L) could significantly disturb the immune response of fish. Moreover, RXRα expression in the liver was significantly altered upon BPA exposure and the trend underlying this change correlated closely with those of the most immune-related parameters, implying the involvement of the PPARγ/RXRα signaling pathway in regulating the immune response of fish upon long-term BPA exposure. In short, our results demonstrate the susceptibility of fish immune system to long-term BPA exposure. Therefore, the immunotoxicity of EDCs in aquatic organisms should not have been underestimated.

The immune system in higher organisms is under integrated control and has the capacity to rapidly... more The immune system in higher organisms is under integrated control and has the capacity to rapidly respond to the environment. Recently, there has been a significant increase in the prevalence of allergic diseases. Environmental factors likely play a major role in the explosion of allergy. Although the "hygiene hypothesis" may explain the increase in allergic diseases which are prone to T helper 2 (Th2) immune responses, recent findings highlight the possible involvement of environmental xenobiotic chemicals which can modulate normal immune function. Interestingly, several reports suggest that the prevalence of systemic lupus erythematosus, a Th2-type autoimmune disease, is also increasing, although the development of high-sensitivity immunological tests may be a possible cause. The increased prevalence of autoimmune disease in women, the sexual dimorphism of the immune response, and the immunomodulatory effects of sex steroids, have focused attention on the role of chemicals which influence sex steroids in the development of immune diseases. Moreover, recent reports indicate that some environmental chemicals can work on nuclear hormone receptors, other than sex hormone receptors, and modulate immune reactions. This review focuses on the impact of environmental chemicals on immune system function and pathogenesis of immune diseases, including allergy and autoimmune diseases.

The endocrine system of wildlife is exposed to a wide variety of natural and man-made chemicals w... more The endocrine system of wildlife is exposed to a wide variety of natural and man-made chemicals which may lead to damage to the reproductive system and other adverse effects, including alteration of drug-metabolizing enzymes. In the present study, the effects of in vivo exposure to a natural (17-estradiol: E2) or a xenoestrogen (4-nonylphenol: NP) estrogen or an anti-estrogen (3,3 ,4,4 ,5-pentachlorobiphenyl: PCB 126) upon vitellogenin (VTG) synthesis and hepatic phase 1 and 2 enzymes have been investigated in adult male sea bass. By means of ELISA analysis with the use of polyclonal antibodies prepared against VTG purified from E2-treated sea bass, we assessed the time course and sensitivity of VTG induction in the plasma of sea bass treated with E2 at 0.1, 0.5, 2.5 and 5.0 mg/kg doses or NP at 5.0 or 50 mg/kg doses, respectively. Sea bass sensitivity to this induction was found to be similar to that of other fish species, but with a delay in maximal response. E2 treatment also caused a selective time-and dose-dependent inhibition of hepatic CYP1A-linked EROD and phase 2 glutathione S-transferase (GST) activities, without affecting the activity of CYP3A-linked 6-testosterone hydroxylase, ()-and (-1)-lauric acid hydroxylases or phase 2 DT-diaphorase. A similar selective inhibition on CYP1A was also observed in fish treated with 50 mg/kg NP. The results regarding CYP1A and CYP3A were also confirmed by Western blot analysis. When the sea bass were treated with either 10 or 100 g/kg PCB 126, an AhR ligand not yet tested in vivo in fish to assess its anti-estrogenicity, a modest and selective induction of EROD and DT-diaphorase activities was observed. Interestingly, both these activities were recovered to their control levels in sea bass co-treated with 0.5 mg/kg E2 and 10 or 100 g/kg PCB 126, probably through a cross-talk mechanism between the estrogen receptor and AhR or other transcription factors that regulate the expression of these enzymes. Furthermore, it was demonstrated that PCB 126 possesses a potent anti-estrogenic activity in the sea bass in vivo as it inhibited the E2-induced VTG synthesis with an IC50 of 28 g/kg. The results of this study suggest that the exposure of fish to xenoestrogens or anti-estrogens

Journal of Aquaculture Development

Acta Biochimica et Biophysica Sinica, 2019

Chemosphere, 2017

Ecotoxicology and environmental safety, Jan 15, 2016

Marine and Freshwater Behaviour and Physiology, 2019

This study investigated the effects of nonylphenol (NP) on hematological and immunological parame... more This study investigated the effects of nonylphenol (NP) on hematological and immunological parameters in both male and female rainbow trout (Oncorhynchus mykiss). Fish were randomly distributed into six groups and administered with NP (10, 50 and 100 μg g -1 week -1 BW) and a single dose of 17-β estradiol (E2; 2 μg g -1 week -1 BW, positive control). The solvent controls received ethanol and coconut oil as a vehicle, while the controls were not injected. Red blood cells (RBCs) count, hematocrit (Hct), hemoglobin (Hb), white blood cells (WBCs), and lymphocytes demonstrated a NP dose-dependent decrease, whereas mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), monocytes, and neutrophils showed an increasing trend in both male and female fish 21 days post-treatment. Also, RBCs, Hb, MCHC, WBCs, and lymphocytes were significantly reduced (p<0.05) in E2 treated fish. Lysozyme, complement components (C3 and C4) and immunoglobulin M (IgM) were increased in fish sera subjected to 10 and 50 μg g -1 NP, while these decreased in groups administered with 100 μg g -1 NP and 2 μg g -1 E2. Except for C4 level at 10 μg g -1 NP, no significant differences were observed in hematological and immunological parameters of male and female in each treatment. Overall, a frequent exposure to NP could lead to adverse effects on fish immunephysiological functions which may cause serious ecological threats of fish natural population sustainability.