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Papers by Kazuaki kikuchi

Research paper thumbnail of Preparation of Water-Soluble Chitin from Alkaline Homogeneous Solution at a Low Temperature

Research paper thumbnail of Composition favorisant la production de collagene et/ou d’acide hyaluronique

Le probleme a resoudre dans le cadre de la presente invention consiste a proposer un nouveau mate... more Le probleme a resoudre dans le cadre de la presente invention consiste a proposer un nouveau materiau sans risque et capable de favoriser de maniere significative la production d’au moins un element choisi dans le groupe constitue par le collagene et l’acide hyaluronique ; et un nouveau materiau qui possede des fonctions empechant l’abaissement de la teneur en humidite dans la couche cornee et ameliorant la rugosite de la peau. La solution proposee est une composition destinee a favoriser la production d’au moins un element choisi dans le groupe constitue par le collagene et l'acide hyaluronique dans des cellules qui contient un hydrolysat de protease de proteine de soja ; et une composition empechant l'abaissement de la teneur en humidite dans la couche cornee et une composition ameliorant la rugosite de la peau contenant chacune un hydrolysat de protease de proteine de soja.

Research paper thumbnail of Separation and physiological functions of anserine from fish extract

More Efficient Utilization of Fish and Fisheries Products - Proceedings of the International Symposium on the occasion of the 70th anniversary of the Japanese Society of Fisheries Science, held in Kyoto, Japan, 7-10 October 2001, 2004

Abstract A separation process that produces anserine powder with a 10.6 % purity and a 9 % yield ... more Abstract A separation process that produces anserine powder with a 10.6 % purity and a 9 % yield from the raw material bonito extract was established. This powder was further purified to anserine hydrochloride with a purity of 98 %. In tests of its physiological effect, anserine that had been administered orally to mice was quickly absorbed into the blood stream and was found to increase the swimming time and the chinning exercise time of the mice. In addition, the content of lactic acid in plasma was lower in mice given anserine than in mice that had not received the dipeptide. These results suggest that anserine has an anti-fatigue effect. The anti-oxidant effect of anserine in vitro by ESR was investigated: anserine, as well as the other imidazole dipeptide carnosine, showed a strong ability to eliminate hydroxyl radicals and singlet oxygens. On the basis of these interesting properties, anserine was considered to have a potential for use in a wide range of applications, including as a component of functional foods and cosmetics.

Research paper thumbnail of Marine Plasmalogen and its Antioxidative Effect Intake of Dietary Fish Oil into Membrane Lipid and Their Stability Against Oxidative Stress

ACS Symposium Series, 1998

Research paper thumbnail of Elastin production-enhancing agents

Research paper thumbnail of Composition Capable of Promoting the Production of Collagen

Research paper thumbnail of Elastin Production-Enhancing Agents

Research paper thumbnail of Peptides that increase collagen or hyaluronic acid production

The present invention relates to a novel peptide or its derivative, or a salt thereof comprising ... more The present invention relates to a novel peptide or its derivative, or a salt thereof comprising the amino acid sequence described above. Furthermore, the invention relates to such a novel composition containing a peptide, etc., use of a method using such as the novel peptides, the novel peptide or the like, polynucleotides encoding the novel peptides. The novel peptide or derivative, or its salts thereof of the present invention can be used to enhance the production in the cell of at least one member selected from the group consisting of collagen and hyaluronic acid. Peptides, collagen, hyaluronic acid,

Research paper thumbnail of Water-Soluble Chitin of Low Degree of Deacetylation

Journal of Carbohydrate Chemistry, 2002

... Xiao Feng Guo,1,* Kazuaki Kikuchi,1 Yoshiharu Matahira,1 Kazuo Sakai,1 and Kozo Ogawa2 ... Wh... more ... Xiao Feng Guo,1,* Kazuaki Kikuchi,1 Yoshiharu Matahira,1 Kazuo Sakai,1 and Kozo Ogawa2 ... When the solution was kept at 5 °C for one day under stirring, it became quite clear without any cloudy appearance. The degree of deacetylation was 23%. ...

Research paper thumbnail of Fatty acid composition of choline and ethanolamine glycerophospholipid subclasses in heart tissue of mammals and migratory and demersal fish

Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 1999

The distribution and fatty acid composition of cardiac choline and ethanolamine glycerophospholip... more The distribution and fatty acid composition of cardiac choline and ethanolamine glycerophospholipids in both migratory and demersal fish and bovine and pig were determined. Phospholipid contents (mg/g heart) were 4.7-9.4 in demersal fish, 14.0-16.5 in migratory fish, and 16.8-20.6 in mammals. Phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were the major components in the phospholipid fraction. Diacyl forms represented 50.2-88.1% of PC in all animals, while plasmalogens comprised 47.0% in bovine, 8.2% in pig and 6.2-7.2% in four species of fish. In PE, plasmalogens varied from 45.0% in bovine and 57.9% in pig to 26.1-29.7% in fish. This glycerophospholipid subclass was identified as containing higher proportions of polyunsaturated fatty acids (PUFAs; 20:4, 20:5, and 22:6) than found in alkylacyl- and diacyl-glycerophospholipids. Qualitative and quantitative differences were found in PE-plasmalogen between land mammals and fish, especially with regard to n-3 fatty acid composition, but no significant difference was noted between migratory and demersal fish.

Research paper thumbnail of Antioxidant Activities of Chitobiose and Chitotriose

Biological and Pharmaceutical Bulletin, 2003

Chitooligosaccharides, the oligomers made up of b b-1,4-linked D-glucosamine, are obtained by par... more Chitooligosaccharides, the oligomers made up of b b-1,4-linked D-glucosamine, are obtained by partial hydrolysis of chitosan, a deacetylation product of chitin. The antioxidant activity of various chitooligosaccharides was tested in vitro with aminoguanidine, pyridoxamine, and Trolox as reference compounds. Hydroxylation of benzoate to salicylate by H 2 O 2 in the presence of Cu 2؉ was effectively inhibited by chitobiose, chitotriose, aminoguanidine, pyridoxamine, and Trolox (their IC 50 values‫,81؍‬ 80, 85, 10, and 95 m mM, respectively), whereas glucosamine and N-acetylchito-oligosaccharides (di-N-acetylchitobiose and tri-N-acetylchitotriose) did not show any inhibitory activity. Chitobiose and chitotriose were more potent than the 3 reference compounds in scavenging hydroxyl radicals produced by photolysis of zinc oxide: IC 50 values of the 2 oligomers were 30 and 55 m mM, respectively. Such a scavenging activity of these 2 chitooligomers was also shown by the use of another system, a mixture of Fe 3؉ /EDTA/ascorbate/H 2 O 2 , for producing hydroxyl radicals. Only chitobiose and Trolox, of the 10 compounds tested, had the ability to scavenge superoxide radicals generated by a non-enzymatic system using phenazine methosulfate and NADH. Taken together with our unpublished observation that chitobiose and chitotriose are appreciably absorbed from the intestine of rats, the present results suggest that these 2 chitooligosaccharides would act as effective antioxidants in vivo when orally ingested.

Research paper thumbnail of Oral N-acetylglucosamine supplementation improves skin conditions of female volunteers: Clinical evaluation by a microscopic three-dimensional skin surface analyzer

Journal of applied cosmetology, 2002

Summary Within the skin ti ssues, acidic mucopolysaccha rides such as hyaluronic acid are present... more Summary Within the skin ti ssues, acidic mucopolysaccha rides such as hyaluronic acid are present in the co­ rium layer and play a large part in water retenti on and skin resili ence. Hyalu ronic acid is a po lymer composed of dimers conta in ing N-acetylglucosamine and g lu curonic acid . Although appli cati ons o f the use of hyaluronic acid in cosmeceutical food ha ve been reported, the beauty efficacy o f ora ll y­ ingested hyaluronic acid cannot be predicted adequate ly because li ttle is known about its digestio n and absorpti on in humans. The purpose of thi s study was to in vesti gate the effect of long-term ora! N-acetylglucosamine sup­ plementati on on skin condi tions in fe ma les who have a common tende ncy of xeroderma and rough skin. The subjects (average age: 25.5 ± 10.7) were assigned rando mly and double -blind to e ither a N-acetylglucosamine group (n= l l ) or a placebo gro up (n= 11 ), and in gested a dail y I 000-mg dose of N-acetylglucosamine or lactose, respe...

Research paper thumbnail of Preparation of Water-Soluble Chitin from Alkaline Homogeneous Solution at a Low Temperature

Research paper thumbnail of Composition favorisant la production de collagene et/ou d’acide hyaluronique

Le probleme a resoudre dans le cadre de la presente invention consiste a proposer un nouveau mate... more Le probleme a resoudre dans le cadre de la presente invention consiste a proposer un nouveau materiau sans risque et capable de favoriser de maniere significative la production d’au moins un element choisi dans le groupe constitue par le collagene et l’acide hyaluronique ; et un nouveau materiau qui possede des fonctions empechant l’abaissement de la teneur en humidite dans la couche cornee et ameliorant la rugosite de la peau. La solution proposee est une composition destinee a favoriser la production d’au moins un element choisi dans le groupe constitue par le collagene et l'acide hyaluronique dans des cellules qui contient un hydrolysat de protease de proteine de soja ; et une composition empechant l'abaissement de la teneur en humidite dans la couche cornee et une composition ameliorant la rugosite de la peau contenant chacune un hydrolysat de protease de proteine de soja.

Research paper thumbnail of Separation and physiological functions of anserine from fish extract

More Efficient Utilization of Fish and Fisheries Products - Proceedings of the International Symposium on the occasion of the 70th anniversary of the Japanese Society of Fisheries Science, held in Kyoto, Japan, 7-10 October 2001, 2004

Abstract A separation process that produces anserine powder with a 10.6 % purity and a 9 % yield ... more Abstract A separation process that produces anserine powder with a 10.6 % purity and a 9 % yield from the raw material bonito extract was established. This powder was further purified to anserine hydrochloride with a purity of 98 %. In tests of its physiological effect, anserine that had been administered orally to mice was quickly absorbed into the blood stream and was found to increase the swimming time and the chinning exercise time of the mice. In addition, the content of lactic acid in plasma was lower in mice given anserine than in mice that had not received the dipeptide. These results suggest that anserine has an anti-fatigue effect. The anti-oxidant effect of anserine in vitro by ESR was investigated: anserine, as well as the other imidazole dipeptide carnosine, showed a strong ability to eliminate hydroxyl radicals and singlet oxygens. On the basis of these interesting properties, anserine was considered to have a potential for use in a wide range of applications, including as a component of functional foods and cosmetics.

Research paper thumbnail of Marine Plasmalogen and its Antioxidative Effect Intake of Dietary Fish Oil into Membrane Lipid and Their Stability Against Oxidative Stress

ACS Symposium Series, 1998

Research paper thumbnail of Elastin production-enhancing agents

Research paper thumbnail of Composition Capable of Promoting the Production of Collagen

Research paper thumbnail of Elastin Production-Enhancing Agents

Research paper thumbnail of Peptides that increase collagen or hyaluronic acid production

The present invention relates to a novel peptide or its derivative, or a salt thereof comprising ... more The present invention relates to a novel peptide or its derivative, or a salt thereof comprising the amino acid sequence described above. Furthermore, the invention relates to such a novel composition containing a peptide, etc., use of a method using such as the novel peptides, the novel peptide or the like, polynucleotides encoding the novel peptides. The novel peptide or derivative, or its salts thereof of the present invention can be used to enhance the production in the cell of at least one member selected from the group consisting of collagen and hyaluronic acid. Peptides, collagen, hyaluronic acid,

Research paper thumbnail of Water-Soluble Chitin of Low Degree of Deacetylation

Journal of Carbohydrate Chemistry, 2002

... Xiao Feng Guo,1,* Kazuaki Kikuchi,1 Yoshiharu Matahira,1 Kazuo Sakai,1 and Kozo Ogawa2 ... Wh... more ... Xiao Feng Guo,1,* Kazuaki Kikuchi,1 Yoshiharu Matahira,1 Kazuo Sakai,1 and Kozo Ogawa2 ... When the solution was kept at 5 °C for one day under stirring, it became quite clear without any cloudy appearance. The degree of deacetylation was 23%. ...

Research paper thumbnail of Fatty acid composition of choline and ethanolamine glycerophospholipid subclasses in heart tissue of mammals and migratory and demersal fish

Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 1999

The distribution and fatty acid composition of cardiac choline and ethanolamine glycerophospholip... more The distribution and fatty acid composition of cardiac choline and ethanolamine glycerophospholipids in both migratory and demersal fish and bovine and pig were determined. Phospholipid contents (mg/g heart) were 4.7-9.4 in demersal fish, 14.0-16.5 in migratory fish, and 16.8-20.6 in mammals. Phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were the major components in the phospholipid fraction. Diacyl forms represented 50.2-88.1% of PC in all animals, while plasmalogens comprised 47.0% in bovine, 8.2% in pig and 6.2-7.2% in four species of fish. In PE, plasmalogens varied from 45.0% in bovine and 57.9% in pig to 26.1-29.7% in fish. This glycerophospholipid subclass was identified as containing higher proportions of polyunsaturated fatty acids (PUFAs; 20:4, 20:5, and 22:6) than found in alkylacyl- and diacyl-glycerophospholipids. Qualitative and quantitative differences were found in PE-plasmalogen between land mammals and fish, especially with regard to n-3 fatty acid composition, but no significant difference was noted between migratory and demersal fish.

Research paper thumbnail of Antioxidant Activities of Chitobiose and Chitotriose

Biological and Pharmaceutical Bulletin, 2003

Chitooligosaccharides, the oligomers made up of b b-1,4-linked D-glucosamine, are obtained by par... more Chitooligosaccharides, the oligomers made up of b b-1,4-linked D-glucosamine, are obtained by partial hydrolysis of chitosan, a deacetylation product of chitin. The antioxidant activity of various chitooligosaccharides was tested in vitro with aminoguanidine, pyridoxamine, and Trolox as reference compounds. Hydroxylation of benzoate to salicylate by H 2 O 2 in the presence of Cu 2؉ was effectively inhibited by chitobiose, chitotriose, aminoguanidine, pyridoxamine, and Trolox (their IC 50 values‫,81؍‬ 80, 85, 10, and 95 m mM, respectively), whereas glucosamine and N-acetylchito-oligosaccharides (di-N-acetylchitobiose and tri-N-acetylchitotriose) did not show any inhibitory activity. Chitobiose and chitotriose were more potent than the 3 reference compounds in scavenging hydroxyl radicals produced by photolysis of zinc oxide: IC 50 values of the 2 oligomers were 30 and 55 m mM, respectively. Such a scavenging activity of these 2 chitooligomers was also shown by the use of another system, a mixture of Fe 3؉ /EDTA/ascorbate/H 2 O 2 , for producing hydroxyl radicals. Only chitobiose and Trolox, of the 10 compounds tested, had the ability to scavenge superoxide radicals generated by a non-enzymatic system using phenazine methosulfate and NADH. Taken together with our unpublished observation that chitobiose and chitotriose are appreciably absorbed from the intestine of rats, the present results suggest that these 2 chitooligosaccharides would act as effective antioxidants in vivo when orally ingested.

Research paper thumbnail of Oral N-acetylglucosamine supplementation improves skin conditions of female volunteers: Clinical evaluation by a microscopic three-dimensional skin surface analyzer

Journal of applied cosmetology, 2002

Summary Within the skin ti ssues, acidic mucopolysaccha rides such as hyaluronic acid are present... more Summary Within the skin ti ssues, acidic mucopolysaccha rides such as hyaluronic acid are present in the co­ rium layer and play a large part in water retenti on and skin resili ence. Hyalu ronic acid is a po lymer composed of dimers conta in ing N-acetylglucosamine and g lu curonic acid . Although appli cati ons o f the use of hyaluronic acid in cosmeceutical food ha ve been reported, the beauty efficacy o f ora ll y­ ingested hyaluronic acid cannot be predicted adequate ly because li ttle is known about its digestio n and absorpti on in humans. The purpose of thi s study was to in vesti gate the effect of long-term ora! N-acetylglucosamine sup­ plementati on on skin condi tions in fe ma les who have a common tende ncy of xeroderma and rough skin. The subjects (average age: 25.5 ± 10.7) were assigned rando mly and double -blind to e ither a N-acetylglucosamine group (n= l l ) or a placebo gro up (n= 11 ), and in gested a dail y I 000-mg dose of N-acetylglucosamine or lactose, respe...