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Papers by matthias kohl

Nutrients

The present study aimed to evaluate the effect of high intensity dynamic resistance exercise (HIT... more The present study aimed to evaluate the effect of high intensity dynamic resistance exercise (HIT-DRT) and whey protein supplementation (WPS) on bone mineral density (BMD) and sarcopenia parameters in osteosarcopenic men. Men ≥ 72 years with osteosarcopenia (n = 43) were randomly assigned to a HIT-RT (HIT-RT: n = 21) or a non-training control group (n = 22). Supervised HIT-RT twice/week was applied for 18 months, while the control group maintained their habitual lifestyle. Supplying WPS, total protein intake amounted to 1.5–1.6 (HIT-RT) and 1.2 g/kg/body mass/d (control). Both groups were supplied with calcium and vitamin D. Primary study outcomes were BMD and the sarcopenia Z-score. After adjusting for multiplicity, we observed significant positive effects for sarcopenia Z-score (standardized mean difference (SMD): 1.40), BMD at lumbar spine (SMD: 0.72) and total hip (SMD: 0.72). In detail, effect sizes for skeletal muscle mass changes were very pronounced (1.97, p < 0.001), whi...

Osteologie

ZusammenfassungZiel dieser Metaanalyse war es (1) den Effekt eines dynamischen Krafttrainings (DR... more ZusammenfassungZiel dieser Metaanalyse war es (1) den Effekt eines dynamischen Krafttrainings (DRT) auf die Knochendichte (BMD) der Lendenwirbelsäule (LWS) einzuschätzen, (2) günstige Belastungs-komponenten zu identifizieren und (3) Trainingsempfehlungen abzuleiten.Eine systematische Literaturrecherche schloss (a) kontrollierte Studien, (b) DRT ≥ 6 Monate mit mindestens einer Trainings- (TG) und einer Kontrollgruppe (KG), (c) LWS-BMD-Messung vor und nach der Intervention und (e) postmenopausale Frauen ein. Subgruppenanalysen wurden für Interventionsdauer, Art des DRT, Trainingshäufigkeit (TH), und -volumen sowie Reizintensität durchgeführt.17 Artikel mit 20 TG und 18 KG konnten eingeschlossen werden. Die standardisierte Mittelwertdifferenz für die LWS-BMD zeigte einen signifikanten Effekt (0,59; 95%-CI: 0,26–0,92). Eine niedrige TH zeigte signifikant höhere Effekte auf die BMD als eine TH von ≥ 2 Einheiten/Woche.Die Meta-Analyse zeigte einen moderaten DRT-Effekt auf die LWS-BMD. Erg...

International Journal of Molecular Sciences

Assessment of hematotoxicity from environmental or xenobiotic compounds is of notable interest an... more Assessment of hematotoxicity from environmental or xenobiotic compounds is of notable interest and is frequently assessed via the colony forming unit (CFU) assay. Identification of the mode of action of single compounds is of further interest, as this often enables transfer of results across different tissues and compounds. Metabolomics displays one promising approach for such identification, nevertheless, suitability with current protocols is restricted. Here, we combined a hematopoietic stem and progenitor cell (HSPC) expansion approach with distinct lineage differentiations, resulting in formation of erythrocytes, dendritic cells and neutrophils. We examined the unique combination of pathway activity in glycolysis, glutaminolysis, polyamine synthesis, fatty acid oxidation and synthesis, as well as glycerophospholipid and sphingolipid metabolism. We further assessed their interconnections and essentialness for each lineage formation. By this, we provide further insights into activ...

Frontiers in Sports and Active Living

Statistics & Probability Letters

Microchimica Acta

For modern approaches in precision medicine, fast and easy-to-use point-of-care diagnostics (POCs... more For modern approaches in precision medicine, fast and easy-to-use point-of-care diagnostics (POCs) are essential. Digoxin was chosen as an example of a drug requiring close monitoring. Digoxin is a cardiac glycoside used for the treatment of tachycardia with a narrow therapeutic window of 0.5-2.0 ng•mL −1 , and toxic effects are common for concentrations above 2.5 ng•mL −1. For monitoring of blood concentration levels and treatment of intoxication, highly selective antibodies for digoxin and its hapten, digoxigenin, are available. A smartphone readout system is described for measuring digoxigenin in human serum using a common gold nanoparticle lateral flow assay (LFA). The R-package GNSplex, which also includes a Shiny app for quantitative test interpretation based on linear models, is used for image analysis. Images of lateral flow strips were taken with an iPhone camera and a simple darkbox made from black cardboard. Sensitivity and accuracy of the quantitative smartphone system as well as analytical parameters such as limit of detection (LOD) were determined and compared to data obtained with a high resolution BioImager. The data show that the smartphone based digoxin assay yields reliable quantitative results within the clinically relevant concentration range.

Scientific Reports

Aptamer-based lateral flow assays (LFAs) are an emerging field of aptamer applications due to num... more Aptamer-based lateral flow assays (LFAs) are an emerging field of aptamer applications due to numerous potential applications. When compared to antibodies, potential advantages like cost effectiveness or lower batch to batch variations are evident. The development of LFAs for small molecules, however, is still challenging due to several reasons, primarily linked to target size and accessible interaction sites. In small molecule analysis, however, aptamers in many cases are preferable since immunogenicity is not required and they may exhibit even higher target selectivity. We report the first cross-recognition of a small molecule (ampicillin) and a protein (C-reactive protein), predicted by in-silico analysis, then experimentally confirmed-using two different aptamers. These features can be exploited for developing an aptamer-based LFA for label-free ampicillin detection, functioning also for analysis in milk extract. Most importantly, the principal setup denotes a novel, transferable and versatile general approach for detection of small molecules using competitive LFAs, unlikely to be generally realized by aptamer-DNA-binding otherwise. Lateral flow assays (LFAs) are simple, fast and cost-effective test systems with high suitability for point-of-care testing (POCT). LFAs are widely applied, including detection of pathogens, food allergens or protein biomarkers of several medical conditions 1-3. They are used for qualitative and to some extent for quantitative monitoring in several areas, including food production or non-laboratory environments 3. LFAs usually employ antigen-antibody binding reaction, combined with lateral fluid flow through a membrane. Antibodies, however, have several limitations; for example, antibodies cannot be raised against toxic or non-immunogenic targets 3. Low-molecular-weight (LMW) compounds, like metabolites or drugs, lacking intrinsic immunogenicity, make it difficult to raise specific antibodies against them. Even though, some antibodies exist against LMW compounds and also have been implemented in LFA applications, their selectivity often remains low, thus limiting LFA applications, which can be overcome by aptamers as probes 4-6. Aptamers are single-stranded DNA or RNA molecules, which can form 3D motifs and selectively bind to corresponding targets. They are comparable to monoclonal antibodies; properties, however, are quite different, since they belong to a different class of biomolecules. The main advantage of aptamers is that, in principle, they can be generated against any desired target, toxic small molecules, non-immunogenic targets or even single molecules, which does not apply to antibodies. Another advantage of aptamers is, that they can be easily and reversibly denaturized by heat or chemicals, due to their robust phosphodiester backbone, a feature which antibodies do not possess. Aptamers are cheap in production, own an extreme low batch-to-batch variation and possess a longer shelf life, compared to antibodies.

PLOS ONE

Surfaces with regular contact with the human body are typically contaminated with microorganisms ... more Surfaces with regular contact with the human body are typically contaminated with microorganisms and might be considered as fomites. Despite spectacles being widespread across populations, little is known about their microbial contamination. Therefore, we swab-sampled 11 worn spectacles within a university setting as well as 10 worn spectacles in a nursing home setting. The microbial load was determined by aerobic cultivation. All spectacles were found to be contaminated with bacteria, with nose pads and ear clips having the highest density, i.e. at sites with direct skin contact. Summed over all sites, the median microbial load of the university spectacles (1.4 ± 10.7 x 10 3 CFU cm-2) did not differ significantly from the spectacles tested in the nursing home (20.8 ± 39.9 x 10 3 CFU cm-2). 215 dominant bacterial morphotypes were analyzed by MALDI biotyping. 182 isolates could be assigned to 10 genera, with Staphylococcus being the most common. On genus-level, bacterial diversity was greater on nursing home spectacles (10 genera) compared to the university environment (2 genera). Four cleaning methods were investigated using lenses artificially contaminated with Escherichia coli, Micrococcus luteus, a 1:2 mixture of E. coli and M. luteus, and Staphylococcus epidermidis (the dominant isolate in our study), respectively. Best cleaning results (99%-100% median germ reduction) were obtained using impregnated wipes; dry cleaning was less effective (85%-90% median germ reduction). Finally, 10 additional worn university spectacles were cleaned with wipes impregnated with an alcohol-free cleaning solution before sampling. The average bacterial load was significantly lower (0.09 ± 0.49 x 10 3 CFU cm-2) compared to the uncleaned university spectacles previously investigated. Spectacles are significantly contaminated with bacteria of mostly human skin origin-including significant amounts of potentially pathogenic ones and may contribute to eye infections as well as fomites in clinical environments.

BMC geriatrics, Mar 9, 2018

Sarcopenic Obesity (SO) is characterized by low lean and high fat mass; i.e. from a functional as... more Sarcopenic Obesity (SO) is characterized by low lean and high fat mass; i.e. from a functional aspect a disproportion between engine (muscle) and mass to be moved (fat). At present, most research focuses on the engine, but the close "cross talk" between age-associated adipose and skeletal muscle tissue inflammation calls for comprehensive interventions that affect both components alike. Protein and exercise are likely candidates, however with respect to the latter, the enthusiasm for intense and frequent exercise is rather low, especially in functionally limited older people. The aim of this study was therefore to evaluate the effect of whole-body electromyostimulation (WB-EMS), a time-efficient, joint-friendly and highly customizable exercise technology, on obesity parameters and cardiometabolic risk in men with SO. One-hundred community-dwelling (cdw) Bavarian men ≥70 years with SO were randomly assigned to either (a) whey protein supplementation (WPS), (b) WB-EMS and pr...

Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme, Jan 18, 2017

To determine the underlying mechanisms after 1 session of (intense) whole-body electromyostimulat... more To determine the underlying mechanisms after 1 session of (intense) whole-body electromyostimulation (WB-EMS) on total energy expenditure (TEE) and resting metabolic rate (RMR), 16 subjects followed a standardized protocol of indirect calorimetry for up to 72 h in 12-h intervals. The single session significantly increased RMR by approximately 25% ± 10% (p < 0.001) and TEE by approximately 9.5% ± 1%, a net effect of ∼460 ± 50 kcal (WB-EMS vs. control group).

Journal of Water and Health

Despite its frequent use in many religious institutions, the microbiological quality of holy wate... more Despite its frequent use in many religious institutions, the microbiological quality of holy water is clearly underinvestigated. We analyzed the microbial load of 54 holy water samples, repeatedly taken in five Roman Catholic churches in the greater area of Villingen-Schwenningen, Germany, by means of aerobic colony counting and Matrix-Assisted Laser Desorption/Ionization (MALDI) Biotyping of representative isolates. Over all samples, colony counting indicated an average aerobic microbial load of 5.85 ± 3.98 × 103 colony forming units (CFU) ml−1 (average ± standard error of the mean (SEM)). Urban churches showed significantly higher contaminations than rural churches, probably owing to a greater number of visitors. Out of 145 bacterial isolates, 63 (43%) were identified to genus level and 39 (27%) to species level. The majority of the identified bacteria were typical human skin commensals, mainly affiliated with the genus Staphylococcus. Ten out of 20 (50%) of the identified species...

Clinical Interventions in Aging

Background: Sarcopenic obesity (SO) is a geriatric syndrome characterized by the disproportion be... more Background: Sarcopenic obesity (SO) is a geriatric syndrome characterized by the disproportion between the amount of lean mass and fat mass. Exercise decreases fat and maintains muscle mass; however, older people fail to exercise at doses sufficient to affect musculoskeletal and cardiometabolic risk factors. The aim of this study was to evaluate the effect of whole-body electromyostimulation (WB-EMS), a time-efficient, joint-friendly and highly individualized exercise technology, on sarcopenia and SO in older men. Materials and methods: A total of 100 community-dwelling northern Bavarian men aged $70 years with sarcopenia and obesity were randomly (1-1-1) assigned to either 16 weeks of 1) WB-EMS and protein supplementation (WB-EMS&P), 2) isolated protein supplementation or 3) nonintervention control. WB-EMS consisted of 1.5×20 min (85 Hz, 350 µs, 4 s of strain to 4 s of rest) applied with moderate-to-high intensity while moving. We further generated a daily protein intake of 1.7-1.8 g/kg/body mass per day. The primary study end point was Sarcopenia Z-Score, and the secondary study end points were body fat rate (%), skeletal muscle mass index (SMI) and handgrip strength. Results: Intention-to-treat analysis determined a significantly favorable effect of WB-EMS&P (P,0.001) and protein (P=0.007) vs control. Both groups significantly (P,0.001) lost body fat (WB-EMS&P: 2.1%; protein: 1.1%) and differed significantly (P#0.004) from control (0.3%). Differences between WB-EMS&P and protein were significant for the Sarcopenia Z-Score (P=0.39) and borderline nonsignificant (P=0.051) for body fat. SMI increased significantly in both groups (P,0.001 and P=0.043) and decreased significantly in the control group (CG; P=0.033); differences between the verum groups and control were significant (P#0.009). Handgrip strength increased in the WB-EMS group (1.90 kg; P,0.001; P=0.050 vs control) only. No adverse effects of WB-EMS or protein supplementation were recorded. Conclusion: WB-EMS&P is a safe and efficient method for tackling sarcopenia and SO in older men. However, the suboptimum effect on functional parameters should be addressed by increased voluntary activation during WB-EMS application.

Journal of Biochips & Tissue Chips

JBTC, an open access journal Biochips J Biochip Tissue chip Given the recent dramatic development... more JBTC, an open access journal Biochips J Biochip Tissue chip Given the recent dramatic development in gain of power togeth

European Journal of Medicinal Chemistry

Ceramide generation is involved in signal transduction of cellular stress response, in particular... more Ceramide generation is involved in signal transduction of cellular stress response, in particular during stress-induced apoptosis in response to stimuli such as minimally modified Low-density lipoproteins, TNFalpha and exogenous C-ceramide. In this paper we describe 48 diverse synthetic products and evaluate their lysosomotropic and acid sphingomyelinase inhibiting activities in macrophages. A stimuli-induced increase of C-ceramide in macrophages can be almost completely suppressed by representative compound NB 06 providing an effective protection of macrophages against apoptosis. Compounds like NB 06 thus offer highly interesting fields of application besides prevention of apoptosis of macrophages in atherosclerotic plaques in vessel walls. Most importantly, they can be used for blocking pH-dependent lysosomal processes and enzymes in general as well as for analyzing lysosomal dependent cellular signaling. Modulation of gene expression of several prominent inflammatory messengers IL1B, IL6, IL23A, CCL4 and CCL20 further indicate potentially beneficial effects in the field of (systemic) infections involving bacterial endotoxins like LPS or infections with influenza A virus.

Menopause (New York, N.Y.), 2017

Multimorbidity related to menopause and/or increased age will put healthcare systems in western n... more Multimorbidity related to menopause and/or increased age will put healthcare systems in western nations under ever-greater strain. Effective strategies to prevent diseases are thus of high priority and should be started earlier in life. The purpose of the study was to evaluate the long-term effect of exercise on different important health parameters in initially early postmenopausal women over a 16-year period. In 1998, 137 early postmenopausal women with osteopenia living in Erlangen-Nürnberg were included in the study. Eighty-six women joined the exercise group (EG) and conducted two supervised group and two home training sessions per week, whereas the control group (CG: n = 51) maintained their physical activity level. Primary outcome parameters were clinical overall fractures incidence; secondary study endpoint was Framingham study-based 10-year risk of coronary death/myocardial infarction and low back pain. In 2014, 59 women of the EG and 46 women of the CG were included in the...

Clinical interventions in aging, 2016

Sarcopenic obesity (SO) is characterized by a combination of low muscle and high fat mass with an... more Sarcopenic obesity (SO) is characterized by a combination of low muscle and high fat mass with an additive negative effect of both conditions on cardiometabolic risk. The aim of the study was to determine the effect of whole-body electromyostimulation (WB-EMS) on the metabolic syndrome (MetS) in community-dwelling women aged ≥70 years with SO. The study was conducted in an ambulatory university setting. Seventy-five community-dwelling women aged ≥70 years with SO living in Northern Bavaria, Germany, were randomly allocated to either 6 months of WB-EMS application with (WB-EMS&P) or without (WB-EMS) dietary supplementation (150 kcal/day, 56% protein) or a non-training control group (CG). WB-EMS included one session of 20 min (85 Hz, 350 μs, 4 s of strain-4 s of rest) per week with moderate-to-high intensity. The primary study endpoint was the MetS Z-score with the components waist circumference (WC), mean arterial pressure (MAP), triglycerides, fasting plasma glucose, and high-densit...

The EPMA journal, 2016

There are more than 8000 rare diseases (RDs) that affect >5 % of the world's population. M... more There are more than 8000 rare diseases (RDs) that affect >5 % of the world's population. Many of the RDs have no effective treatment and lack of knowledge creates delayed diagnosis making management difficult. The emerging concept of the personalized medicine allows for early screening, diagnosis, and individualized treatment of human diseases. In this context, the discovery of biomarkers in RDs will be of prime importance to enable timely prevention and effective treatment. Since 80 % of RDs are of genetic origin, identification of new genes and causative mutations become valuable biomarkers. Furthermore, dynamic markers such as expressed genes, metabolites, and proteins are also very important to follow prognosis and response the therapy. Recent advances in omics technologies and their use in combination can define pathophysiological pathways that can be drug targets. Biomarker discovery and their use in diagnosis in RDs is a major pillar in RD research.

International journal of molecular sciences, Jan 15, 2017

Cardiac dysfunction, in particular of the left ventricle, is a common and early event in sepsis, ... more Cardiac dysfunction, in particular of the left ventricle, is a common and early event in sepsis, and is strongly associated with an increase in patients' mortality. Acid sphingomyelinase (SMPD1)-the principal regulator for rapid and transient generation of the lipid mediator ceramide-is involved in both the regulation of host response in sepsis as well as in the pathogenesis of chronic heart failure. This study determined the degree and the potential role to which SMPD1 and its modulation affect sepsis-induced cardiomyopathy using both genetically deficient and pharmacologically-treated animals in a polymicrobial sepsis model. As surrogate parameters of sepsis-induced cardiomyopathy, cardiac function, markers of oxidative stress as well as troponin I levels were found to be improved in desipramine-treated animals, desipramine being an inhibitor of ceramide formation. Additionally, ceramide formation in cardiac tissue was dysregulated in SMPD1(+/+) as well as SMPD1(-/-) animals, ...

International Journal of Statistics in Medical Research, Dec 20, 2012

We give a brief overview of feature selection methods used in statistical classification. We cove... more We give a brief overview of feature selection methods used in statistical classification. We cover filter, wrapper and embedded methods.