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Papers by kee yoeup paek

Research paper thumbnail of Assessment of the Limitations of Somatic Hybidization

Research paper thumbnail of Mass Propagation of Peanut (Arachis hypogaea L.) Plant through Cultured Seeds in Vitro

Japanese journal of tropical agriculture, 1989

Micropropagation of peanut plant was examined through cultured seeds in vitro. Multiple shoot bud... more Micropropagation of peanut plant was examined through cultured seeds in vitro. Multiple shoot buds were induced from peanut seedlings by BAP in vitro. Multile shoot buds formed from nodal region. Shoots were continuously multiplied by using combinations of BAP and IBA. One can calculate that more than 1,600,000 shoots could be obtained from a single shoot in a year using the present technique. Plant regeneration was achieved by rooting single shoots. The rooting rate was 50% at IBA free and 100% at all concentrations of IBA used. Regenerated plantlets were successfully planted out in soil via semi-aquaculture condition.

Research paper thumbnail of A comprehensivein silicoexploration of pharmacological properties, bioactivities and COX-2 inhibitory potential of eleutheroside B fromEleutherococcus senticosus(Rupr. & Maxim.) Maxim

Journal of Biomolecular Structure and Dynamics, 2020

Research paper thumbnail of Effects of Various Bioreactors on Growth and Ginsenoside Accumulation in Ginseng Adventitious Root Cultures(Panax ginseng C.A. Meyer)

Journal of Plant Biotechnology, 2004

Research paper thumbnail of Reversion of inflorescence development inEuphorbia milliiand its application to large-scale micropropagation in an air-lift bioreactor

The Journal of Horticultural Science and Biotechnology, 2005

Résumé/Abstract An efficient, simple micropropagation protocol was developed for Euphorbia millii... more Résumé/Abstract An efficient, simple micropropagation protocol was developed for Euphorbia millii Des Moulines using inflorescence explants and an automated, low cost, air-lift bioreactor system. Inflorescence meristems of the main axis usually reverted to ...

Research paper thumbnail of Effects of ABA, reduced nitrogen source and osmoticum for somatic embryogenesis in Liriodendron tulipifera

Journal of Plant Biotechnology, 2011

Research paper thumbnail of Auxin and Cytokinin Affect Biomass and Bioactive Compound Production from Adventitious Roots of Eleutherococcus koreanum

Wonye kwahak kisulchi = Korean journal of horticultural science and technology /

In an attempt to improve biomass and bioactive compound production, we cultured adventitious root... more In an attempt to improve biomass and bioactive compound production, we cultured adventitious roots of in 250 mL Erlenmeyer flasks using Murashige and Skoog (MS) medium with different concentrations of auxins (IBA, NAA, IAA) and cytokinins (BA, kinetin, TDZ). Root biomass (fresh and dry weight) was enhanced at indole-3-butyric acid (IBA) after 5 weeks of culture. The content of total phenolics and flavonoids was also increased with IBA compared to -naphtalene acetic acid (NAA) or indole-3-acetic acid (IAA) treatments. The combination of IBA with thidiazuron (TDZ; N-phenyl-N'-1,2,3,-thidiazol-5-ylurea) enhanced root fresh and dry weight (1.4- and 1.6-fold, respectively) as well as the content of total phenolics and flavonoids compared to the relative control (without cytokinin). On the contrary, -benzyladenine (BA) and 6-furfurylaminopurine (kinetin) did not significantly affect root biomass and bioactive compound production in adventitious roots of . These results suggested that ...

Research paper thumbnail of 철피석곡의 원괴체상구체 및 다신초 추출물의 생리활성에 관한 비교 연구

Journal of the Society of Cosmetic Scientists of Korea, 2014

Research paper thumbnail of In Vitro Cell. Dev. Biol.-2003

Research paper thumbnail of Growth and Flowering Response of Calla(Zantedeschia)Base on Shading Rates in Highland

Korean Society for Floricultural Science, 2012

Research paper thumbnail of Production of Caffeic Acid Derivatives from Adventitious Root Cultures of Echinacea purpurea (L.) Moench

Production of Biomass and Bioactive Compounds Using Bioreactor Technology, 2014

Echinacea purpurea (L.) Moench, the purple cone flower is one of the world’s most important medic... more Echinacea purpurea (L.) Moench, the purple cone flower is one of the world’s most important medicinal herbs and active ingredients of purple cone flower are caffeic acid derivatives namely caftaric acid, chlorogenic acid, caffeic acid, cynarin, echinacoside and cichoric acid. Efforts have been made in the recent past for the production of caffeic acid derivatives from adventitious root cultures. Bioreactor cultures have been established for large-scale production of biomass and bioactive compounds and various physiological parameters affecting the biomass and accumulation of caffeic acid derivatives have been investigated. Advances in adventitious root cultures of Echinacea purpurea for the production of bioactive compounds have been summarized in this review.

Research paper thumbnail of Food Ingredients from Plant Cell, Tissue and Organ Cultures: Bio-safety and Efficacy Evaluations

Production of Biomass and Bioactive Compounds Using Bioreactor Technology, 2014

Plants are valuable source of a wide range of secondary metabolites, which are used as pharmaceut... more Plants are valuable source of a wide range of secondary metabolites, which are used as pharmaceuticals, flavors, fragrances, coloring agents and food additives. Various bioactive compounds are produced these days through plant cell, tissue and organ cultures (PCTOC) and the diverse products which are derived from PCTOC are available in the market as pharmaceuticals and food ingredients. Even though PCTOC products are of in vitro origin, they possess many components other than targeted compounds, and sometimes these components may be toxic, thus making the biosafety evaluations necessary for the PCTOC raw materials/products. Currently, well framed biosafety evaluation methods/procedures are not available for PCTOC raw materials/products. In this chapter, we have discussed various methods proposed by scientists and we have put forward a general criterion for evaluation of PCTOC products. We have also discussed two specific examples namely, tissue cultured mountain ginseng adventitious roots (TCMGARs) and tissue cultured Echinacea purpurea adventitious roots (TCEPARs) to illustrate the various steps involved in the process of safety evaluation.

Research paper thumbnail of Number of air exchanges, sucrose concentration, photosynthetic photon flux, and differences in photoperiod and dark period temperatures affect growth of Rehmannia glutinosa plantlets in vitro

Plant Cell Tissue and Organ Culture, 2000

Rehmannia glutinosa plantlets were cultured for 4 weeks under different culture conditions to det... more Rehmannia glutinosa plantlets were cultured for 4 weeks under different culture conditions to determine the optimum environment for in vitro growth and ex vitro survival. Plantlet growth increased with an increasing number of air exchanges of the culture vessel, exhibiting greatest shoot weight, total fresh weight, leaf area, and chlorophyll content at 4.4 h−1 of air exchanges. High sucrose concentration

Research paper thumbnail of FISH and GISH analysis of the genomic relationships amongPanax species

Genes & Genomics, 2009

Research paper thumbnail of Growth, secondary metabolite production and antioxidant enzyme response of Morinda citrifolia adventitious root as affected by auxin and cytokinin

Plant Biotechnology Reports, 2010

Morinda citrifolia adventitious roots were cultured in shake flasks using Murashige and Skoog med... more Morinda citrifolia adventitious roots were cultured in shake flasks using Murashige and Skoog medium with different types and concentrations of auxin and cytokinin. Root (fresh weight and dry weight) accumulation was enhanced at 5 mg l−1 indole butyric acid (IBA) and at 7 and 9 mg l−1 naphthalene acetic acid (NAA). On the other hand, 9 mg l−1 NAA decreased the anthraquinone, phenolic and flavonoid contents

Research paper thumbnail of Photon flux density and light quality induce changes in growth, stomatal development, photosynthesis and transpiration of Withania Somnifera (L.) Dunal. plantlets

Plant Cell, Tissue and Organ Culture, 2007

Research paper thumbnail of Copper-induced changes in the growth, oxidative metabolism, and saponin production in suspension culture roots of Panax ginseng in bioreactors

Research paper thumbnail of Internal air current patterns depend on the ventilation method in a scaled-up culture vessel for micropropagation

Journal of Plant Biology, 2006

ABSTRACT

Research paper thumbnail of Nitric Oxide Elicitation Induces the Accumulation of Secondary Metabolites and Antioxidant Defense in Adventitious Roots ofEchinacea purpurea

Journal of Plant Biology, 2007

Abstract Nitric oxide and reactive oxygen species are important signal molecules that play key ro... more Abstract Nitric oxide and reactive oxygen species are important signal molecules that play key roles in plant defense responses. We investigated the involvement of nitric oxide elicitation in the synthesis of secondary metabolites within the adventitious roots ...

Research paper thumbnail of The effects of spaceflight on soluble protein, isoperoxidase, and genomic DNA in ural licorice (Glycyrrhiza uralensis fisch.)

Journal of Plant Biology, 2000

Research paper thumbnail of Assessment of the Limitations of Somatic Hybidization

Research paper thumbnail of Mass Propagation of Peanut (Arachis hypogaea L.) Plant through Cultured Seeds in Vitro

Japanese journal of tropical agriculture, 1989

Micropropagation of peanut plant was examined through cultured seeds in vitro. Multiple shoot bud... more Micropropagation of peanut plant was examined through cultured seeds in vitro. Multiple shoot buds were induced from peanut seedlings by BAP in vitro. Multile shoot buds formed from nodal region. Shoots were continuously multiplied by using combinations of BAP and IBA. One can calculate that more than 1,600,000 shoots could be obtained from a single shoot in a year using the present technique. Plant regeneration was achieved by rooting single shoots. The rooting rate was 50% at IBA free and 100% at all concentrations of IBA used. Regenerated plantlets were successfully planted out in soil via semi-aquaculture condition.

Research paper thumbnail of A comprehensivein silicoexploration of pharmacological properties, bioactivities and COX-2 inhibitory potential of eleutheroside B fromEleutherococcus senticosus(Rupr. & Maxim.) Maxim

Journal of Biomolecular Structure and Dynamics, 2020

Research paper thumbnail of Effects of Various Bioreactors on Growth and Ginsenoside Accumulation in Ginseng Adventitious Root Cultures(Panax ginseng C.A. Meyer)

Journal of Plant Biotechnology, 2004

Research paper thumbnail of Reversion of inflorescence development inEuphorbia milliiand its application to large-scale micropropagation in an air-lift bioreactor

The Journal of Horticultural Science and Biotechnology, 2005

Résumé/Abstract An efficient, simple micropropagation protocol was developed for Euphorbia millii... more Résumé/Abstract An efficient, simple micropropagation protocol was developed for Euphorbia millii Des Moulines using inflorescence explants and an automated, low cost, air-lift bioreactor system. Inflorescence meristems of the main axis usually reverted to ...

Research paper thumbnail of Effects of ABA, reduced nitrogen source and osmoticum for somatic embryogenesis in Liriodendron tulipifera

Journal of Plant Biotechnology, 2011

Research paper thumbnail of Auxin and Cytokinin Affect Biomass and Bioactive Compound Production from Adventitious Roots of Eleutherococcus koreanum

Wonye kwahak kisulchi = Korean journal of horticultural science and technology /

In an attempt to improve biomass and bioactive compound production, we cultured adventitious root... more In an attempt to improve biomass and bioactive compound production, we cultured adventitious roots of in 250 mL Erlenmeyer flasks using Murashige and Skoog (MS) medium with different concentrations of auxins (IBA, NAA, IAA) and cytokinins (BA, kinetin, TDZ). Root biomass (fresh and dry weight) was enhanced at indole-3-butyric acid (IBA) after 5 weeks of culture. The content of total phenolics and flavonoids was also increased with IBA compared to -naphtalene acetic acid (NAA) or indole-3-acetic acid (IAA) treatments. The combination of IBA with thidiazuron (TDZ; N-phenyl-N'-1,2,3,-thidiazol-5-ylurea) enhanced root fresh and dry weight (1.4- and 1.6-fold, respectively) as well as the content of total phenolics and flavonoids compared to the relative control (without cytokinin). On the contrary, -benzyladenine (BA) and 6-furfurylaminopurine (kinetin) did not significantly affect root biomass and bioactive compound production in adventitious roots of . These results suggested that ...

Research paper thumbnail of 철피석곡의 원괴체상구체 및 다신초 추출물의 생리활성에 관한 비교 연구

Journal of the Society of Cosmetic Scientists of Korea, 2014

Research paper thumbnail of In Vitro Cell. Dev. Biol.-2003

Research paper thumbnail of Growth and Flowering Response of Calla(Zantedeschia)Base on Shading Rates in Highland

Korean Society for Floricultural Science, 2012

Research paper thumbnail of Production of Caffeic Acid Derivatives from Adventitious Root Cultures of Echinacea purpurea (L.) Moench

Production of Biomass and Bioactive Compounds Using Bioreactor Technology, 2014

Echinacea purpurea (L.) Moench, the purple cone flower is one of the world’s most important medic... more Echinacea purpurea (L.) Moench, the purple cone flower is one of the world’s most important medicinal herbs and active ingredients of purple cone flower are caffeic acid derivatives namely caftaric acid, chlorogenic acid, caffeic acid, cynarin, echinacoside and cichoric acid. Efforts have been made in the recent past for the production of caffeic acid derivatives from adventitious root cultures. Bioreactor cultures have been established for large-scale production of biomass and bioactive compounds and various physiological parameters affecting the biomass and accumulation of caffeic acid derivatives have been investigated. Advances in adventitious root cultures of Echinacea purpurea for the production of bioactive compounds have been summarized in this review.

Research paper thumbnail of Food Ingredients from Plant Cell, Tissue and Organ Cultures: Bio-safety and Efficacy Evaluations

Production of Biomass and Bioactive Compounds Using Bioreactor Technology, 2014

Plants are valuable source of a wide range of secondary metabolites, which are used as pharmaceut... more Plants are valuable source of a wide range of secondary metabolites, which are used as pharmaceuticals, flavors, fragrances, coloring agents and food additives. Various bioactive compounds are produced these days through plant cell, tissue and organ cultures (PCTOC) and the diverse products which are derived from PCTOC are available in the market as pharmaceuticals and food ingredients. Even though PCTOC products are of in vitro origin, they possess many components other than targeted compounds, and sometimes these components may be toxic, thus making the biosafety evaluations necessary for the PCTOC raw materials/products. Currently, well framed biosafety evaluation methods/procedures are not available for PCTOC raw materials/products. In this chapter, we have discussed various methods proposed by scientists and we have put forward a general criterion for evaluation of PCTOC products. We have also discussed two specific examples namely, tissue cultured mountain ginseng adventitious roots (TCMGARs) and tissue cultured Echinacea purpurea adventitious roots (TCEPARs) to illustrate the various steps involved in the process of safety evaluation.

Research paper thumbnail of Number of air exchanges, sucrose concentration, photosynthetic photon flux, and differences in photoperiod and dark period temperatures affect growth of Rehmannia glutinosa plantlets in vitro

Plant Cell Tissue and Organ Culture, 2000

Rehmannia glutinosa plantlets were cultured for 4 weeks under different culture conditions to det... more Rehmannia glutinosa plantlets were cultured for 4 weeks under different culture conditions to determine the optimum environment for in vitro growth and ex vitro survival. Plantlet growth increased with an increasing number of air exchanges of the culture vessel, exhibiting greatest shoot weight, total fresh weight, leaf area, and chlorophyll content at 4.4 h−1 of air exchanges. High sucrose concentration

Research paper thumbnail of FISH and GISH analysis of the genomic relationships amongPanax species

Genes & Genomics, 2009

Research paper thumbnail of Growth, secondary metabolite production and antioxidant enzyme response of Morinda citrifolia adventitious root as affected by auxin and cytokinin

Plant Biotechnology Reports, 2010

Morinda citrifolia adventitious roots were cultured in shake flasks using Murashige and Skoog med... more Morinda citrifolia adventitious roots were cultured in shake flasks using Murashige and Skoog medium with different types and concentrations of auxin and cytokinin. Root (fresh weight and dry weight) accumulation was enhanced at 5 mg l−1 indole butyric acid (IBA) and at 7 and 9 mg l−1 naphthalene acetic acid (NAA). On the other hand, 9 mg l−1 NAA decreased the anthraquinone, phenolic and flavonoid contents

Research paper thumbnail of Photon flux density and light quality induce changes in growth, stomatal development, photosynthesis and transpiration of Withania Somnifera (L.) Dunal. plantlets

Plant Cell, Tissue and Organ Culture, 2007

Research paper thumbnail of Copper-induced changes in the growth, oxidative metabolism, and saponin production in suspension culture roots of Panax ginseng in bioreactors

Research paper thumbnail of Internal air current patterns depend on the ventilation method in a scaled-up culture vessel for micropropagation

Journal of Plant Biology, 2006

ABSTRACT

Research paper thumbnail of Nitric Oxide Elicitation Induces the Accumulation of Secondary Metabolites and Antioxidant Defense in Adventitious Roots ofEchinacea purpurea

Journal of Plant Biology, 2007

Abstract Nitric oxide and reactive oxygen species are important signal molecules that play key ro... more Abstract Nitric oxide and reactive oxygen species are important signal molecules that play key roles in plant defense responses. We investigated the involvement of nitric oxide elicitation in the synthesis of secondary metabolites within the adventitious roots ...

Research paper thumbnail of The effects of spaceflight on soluble protein, isoperoxidase, and genomic DNA in ural licorice (Glycyrrhiza uralensis fisch.)

Journal of Plant Biology, 2000