kyriakos papadopoulos - Academia.edu (original) (raw)
Papers by kyriakos papadopoulos
Biomolecules
The anticancer activity of acridone derivatives has attracted increasing interest, therefore, a v... more The anticancer activity of acridone derivatives has attracted increasing interest, therefore, a variety of substituted analogs belonging to this family have been developed and evaluated for their anti-cancer properties. A series of N-alkyl-acridones 1–6 and N,N′-dialkyl-9,9′-biacridylidenes 7–12 with variable alkyl chains were examined for their topoisomerase I activity at neutral and acidic conditions as well as for their binding capacity to calf thymus and possible radical trapping antioxidant activity. It was found that at a neutral pH, topoisomerase I activity of both classes of compounds was similar, while under acidic conditions, enhanced intercalation was observed. N-alkyl-acridone derivatives 1–6 exhibited stronger, dose-dependent, cytotoxic activity against MCF-7 human breast epithelial cancer cells than N,N′-dialkyl-9,9′-biacridylidenes 7–12, revealing that conjugation of the heteroaromatic system plays a significant role on the effective distribution of the compound in th...
Part of this work deals with the isolation and structure elucidation of the main polar secondary ... more Part of this work deals with the isolation and structure elucidation of the main polar secondary metabolites of the aerial parts of Retama raetam (Forssk.) Webb & Berthel, as well as the evaluation of their potential analgesic properties, while the rest deals with the antioxidant activities of the aqueous extracts of roots, stem, fruits and flowers of the plant. It was found that the isoflavones genistein 1, 6-hydroxygenistein 2, 3'-O-methylorobol 3, pratensein 4, biochanin A 8, the flavones 6-hydroxyapigenin 7 and luteolin 5, the flavonol kaempferol 6, as well as the phenolic compound p-coumaric acid 9 reduce significantly the pain at a concentration dose of 1 mg/kg. The most active compounds were 3 and 8 (86.19% and 75.23%, respectively). The obtained aqueous extracts of R. raetam were also evaluated for their antioxidant activities using two different photometric methods; the results revealed that all extracts exerted very low free radical scavenging activity compared to the ...
Analytica Chimica Acta, 2001
A sensitive and simple method for measuring antioxidant activity was developed, based on photosto... more A sensitive and simple method for measuring antioxidant activity was developed, based on photostoragechemiluminescence generated from photolyzed acridine and strong bases. This method was used to evaluate the antioxidant activity of food additives by measuring the inhibition of chemiluminescence at a gradient of antioxidant concentrations. Adipic, tartaric, citric, ascorbic, malic and fumaric acid were employed as antioxidants. The method is more suitable for lipophilic compounds.
Cancers
Increased rates of reactive oxygen/nitrogen species (ROS/RNS) are involved in almost all cancer t... more Increased rates of reactive oxygen/nitrogen species (ROS/RNS) are involved in almost all cancer types, associated with tumor development and progression, causing damage to biomolecules such as proteins, nucleic acids and membrane lipids, in different biological compartments. We used a human tumor xenograft mouse model to evaluate for the first time in parallel the remodeling of fatty acid moieties in erythrocyte membrane phospholipids and the level of ROS-induced DNA lesions in liver and kidney tissues. Using liquid chromatography tandem mass spectrometry the 5’R and 5’S diastereoisomers of 5’,8-cyclo-2’-deoxyadenosine and 5’,8-cyclo-2’-deoxyguanosine, together with 8-oxo-7,8-dihydro-2’-deoxyadenosine, were determined in mice at young (4- and 5-weeks) and old (17-weeks) ages and compared with control SCID mice without tumor implantation. Tumor-bearing mice showed a higher level of ROS-damaged nucleosides in genomic DNA as the age and tumor progress, compared to controls (1.07–1.53-f...
Molecules
The use of liposomes has been crucial for investigations in biomimetic chemical biology as a memb... more The use of liposomes has been crucial for investigations in biomimetic chemical biology as a membrane model and in medicinal chemistry for drug delivery. Liposomes are made of phospholipids whose biophysical characteristics strongly depend on the type of fatty acid moiety, where natural unsaturated lipids always have the double bond geometry in the cis configuration. The influence of lipid double bond configuration had not been considered so far with respect to the competence of liposomes in delivery. We were interested in evaluating possible changes in the molecular properties induced by the conversion of the double bond from cis to trans geometry. Here we report on the effects of the addition of trans-phospholipids supplied in different amounts to other liposome constituents (cholesterol, neutral phospholipids and cationic surfactants), on the size, ζ-potential and stability of liposomal formulations and on their ability to encapsulate two dyes such as rhodamine B and fluorescein. From a biotechnological point of view, trans-containing liposomes proved to have different characteristics from those containing the cis analogues, and to influence the incorporation and release of the dyes. These results open new perspectives in the use of the unnatural lipid geometry, for the purpose of changing liposome behavior and/or of obtaining molecular interferences, also in view of synergic effects of cell toxicity, especially in antitumoral strategies.
Journal of Photochemistry and Photobiology A: Chemistry
ABSTRACT 6,8-Diphenylimidazopyrazinone derivatives having a substituent R (R=CF3, H, and OMe) at ... more ABSTRACT 6,8-Diphenylimidazopyrazinone derivatives having a substituent R (R=CF3, H, and OMe) at pare position of the 8-phenyl group were synthesized and their chemiluminescent properties were investigated. The chemiluminescence maxima (CLmax) of these compounds were observed to be in the range of 513-553 nm with a bathochromic shift that increased with the electron-withdrawing character of R, contrary to the previously observed substituent effect at the 6-postion. The chemiluminescence efficiencies (phi(CL)) of these imidazopyrazinones were improved by the introduction of a p-substituted phenyl group at the 8-position. The quantitative investigation of the three quantum efficiencies (phi(R), phi(S), and phi(FL)) whose product gives us phi(CL) revealed that the phi(CL) gains made were largely because of the increase in the values of the fluorescence quantum yields of the corresponding light emitters (phi(FL)). The yields of the singlet-excited emitters (phi(S)) during the chemiluminescent reaction were found to be very small (0.015-0.019), suggesting that one cannot construct an efficient imidazopyrazinone-chemiluminescence system that is comparable to the aequorin bioluminescence system only by using the electronic effects of substituents.
Free Radical Research
5',8-Cyclo-2'-deoxyadenosine (cdA) and 5&... more 5',8-Cyclo-2'-deoxyadenosine (cdA) and 5',8-cyclo-2'-deoxyguanosine (cdG) in their two diastereomeric forms, 5'S and 5'R, are tandem lesions produced by the attack of hydroxyl radicals to the purine moieties of DNA. Their formation has been found to challenge the cells' repair machinery, initiating the nucleotide excision repair (NER) for restoring the genome integrity. The involvement of oxidatively induced DNA damage in carcinogenesis and the reduced capacity of some cancer cell lines to repair oxidised DNA base lesions, intrigued us to investigate the implication of these lesions in breast cancer, the most frequently occurring cancer in women. Using liquid chromatography tandem mass spectrometry (LC-MS/MS), we measured the levels of diastereomeric cdA's and cdG's in estrogen receptor-alpha positive (ER-α) MCF-7 and triple negative MDA-MB-231 breast cancer cell lines before and after exposure to two different conditions: ionising radiations and hydrogen peroxide, followed by an interval period to allow DNA repair. An increase at the measured levels of all four lesions, i.e. 5'S-cdA, 5'R-cdA, 5'S-cdG and 5'R-cdG, was observed either after γ-irradiation (5 Gy dose) or hydrogen peroxide treatment (300 μM) compared to the untreated cells (control), independently from the length of the interval period for repair. For comparison reasons, we also measured the levels of 8-oxo-2'-deoxyadenosine (8-oxo-dA), a well-known oxidatively induced DNA damage lesion and base excision repair (BER) substrate. The collected data indicate that MCF-7 and MDA-MB-231 breast cancer cells are highly susceptible to radiation-induced DNA damage, being mainly defective in the repair of these lesions.
Inorganic Chemistry
We report on the synthesis, characterization and optoelectronic properties of the novel trimethyl... more We report on the synthesis, characterization and optoelectronic properties of the novel trimethylsulfonium lead triiodide perovskite, (CH 3) 3 SPbI 3. At room temperature, the air-stable compound adopts a hexagonal crystal structure with a 1D network of face-sharing [PbI 6 ] octahedra along the c axis. UV-Vis reflectance spectroscopy on polycrystalline films revealed a band gap of 2.5 eV in good agreement with first-principles calculations, which show a small separation between direct and indirect band gaps. Electrical resistivity measurements on single crystals indicated that the compound behaves as a semi-insulator. According to multi-temperature single-crystal X-ray diffraction, synchrotron powder X-ray diffraction, Raman spectroscopy and differential scanning calorimetry, two fully reversible structural phase transitions occur at-5 °C and ca.-100 °C with reduction of the unit cell symmetry to monoclinic as temperature decreases. The role of the trimethylsulfonium cation regarding the chemical stability and optoelectronic properties of the new compound is discussed in comparison with APbI 3 (A = Cs, methylammonium, and formamidinium cation) that are most commonly used in perovskite solar cells.
Http Dx Doi Org 10 1080 00032719 2011 649456, Mar 1, 2012
Six well established spectrophotometric assays (Folin-Ciocalteau, DPPH, ABTS •+ , FRAP, CUPRAC an... more Six well established spectrophotometric assays (Folin-Ciocalteau, DPPH, ABTS •+ , FRAP, CUPRAC and the o-diphenols method) have been complementary implemented in order to estimate the total phenolic and o-diphenolic content as well as the free radical scavenging and reducing antioxidant capacities of forty white and ten red wines of different varieties and geographic origin. In white wines, the results were weakly correlated, whereas the results in red wines were strongly correlated, therefore postulating their separate use in order to estimate efficiently the correlation between results of these methods. The results were subjected to the unsupervised PCA pattern recognition method to investigate the possible classification of white wines. PC analysis accomplished to frame the wine samples in clear clusters, when the extracted PCA model was based on the results of all six spectrophotometric assays. The usefulness of implementing assays based on different antioxidant mechanisms is discussed. To our knowledge, this is the first time that the different responses of antioxidants to these spectrophotometric assays classified white wines according to their variety.
Http Dx Doi Org 10 1080 00032719 2011 649461, Apr 13, 2012
Journal für praktische Chemie
Chemistry of Natural Compounds
Photochemistry and Photobiology, 2015
A new family of Ru(II)-based photosensitizers was synthesized and systematically characterized. T... more A new family of Ru(II)-based photosensitizers was synthesized and systematically characterized. The ligands employed to coordinate the ruthenium metal center were the commercially available 2,2'-bipyridine and a pyridine-quinoline hybrid bearing an anthracene moiety. The complexes obtained carry either PF6- or Cl(-) counterions. These counterions determine the complexes' hydrophobic or hydrophilic character, respectively, therefore dictating their solubility in biologically related media. All photosensitizers exhibit characteristic, relatively strong and wide UV-Vis absorption spectral profiles. Their high efficiency in generating cytotoxic singlet oxygen was established (up to ΦΔ ~0.8). Moreover, the interaction of these photosensitizers with double-stranded DNA was studied fluoro- and photospectroscopically and their binding affinities were found to be of the order of 3 × 10(7) M(-1) . All complexes are photocytotoxic to DU145 human prostate cancer cells. The highest light-induced toxicity was conferred by the photosensitizers bearing Cl(-) counterions, probably due to the looser ionic "chaperoning" of Cl(-) , in comparison to PF6-, leading to higher cell internalization.
European Journal of Inorganic Chemistry
Activités anti-radicalaires de l'huile essentielle et des extraits bruts de
Microchemical Journal, 2015
ABSTRACT The antioxidant activity of oils is an important parameter that determines oils'... more ABSTRACT The antioxidant activity of oils is an important parameter that determines oils' nutritional value and quality. To date, the methods for the determination of the antioxidant activity of oils are performed under batch conditions, therefore, they have large turnaround times and are labor-intensive. In this work, we describe the first automated method for the determination of the antioxidant activity of edible oils by resorting to the benefits of Flow Injection Analysis (FIA) and the high sensitivity of chemiluminescence (CL) detection. In the developed method, we used the chemiluminescent reaction of bis(2,4, 6-trichlorophenyl) oxalate with the reactive oxygen species produced by the catalytic decomposition of H2O2 by Mn(II) ions. The antioxidants present in the sample scavenge the reactive oxygen species and inhibit the light reaction. 1-Amino-pyrene was used as fluorophore and 1-methyl-imidazole as base catalyst in the reaction. Method validation and application to real samples showed that the analytical features of the method are very satisfactory in terms of linearity of response-lines of standard antioxidant compounds (R2 = 0.98–0.999), precision (%RSD = 0.85–1.9%) and accuracy (%recovery = 91–109%), suggesting that the developed assay could serve as an efficient tool to the routine screening of antioxidant properties in edible oils.
Biomolecules
The anticancer activity of acridone derivatives has attracted increasing interest, therefore, a v... more The anticancer activity of acridone derivatives has attracted increasing interest, therefore, a variety of substituted analogs belonging to this family have been developed and evaluated for their anti-cancer properties. A series of N-alkyl-acridones 1–6 and N,N′-dialkyl-9,9′-biacridylidenes 7–12 with variable alkyl chains were examined for their topoisomerase I activity at neutral and acidic conditions as well as for their binding capacity to calf thymus and possible radical trapping antioxidant activity. It was found that at a neutral pH, topoisomerase I activity of both classes of compounds was similar, while under acidic conditions, enhanced intercalation was observed. N-alkyl-acridone derivatives 1–6 exhibited stronger, dose-dependent, cytotoxic activity against MCF-7 human breast epithelial cancer cells than N,N′-dialkyl-9,9′-biacridylidenes 7–12, revealing that conjugation of the heteroaromatic system plays a significant role on the effective distribution of the compound in th...
Part of this work deals with the isolation and structure elucidation of the main polar secondary ... more Part of this work deals with the isolation and structure elucidation of the main polar secondary metabolites of the aerial parts of Retama raetam (Forssk.) Webb & Berthel, as well as the evaluation of their potential analgesic properties, while the rest deals with the antioxidant activities of the aqueous extracts of roots, stem, fruits and flowers of the plant. It was found that the isoflavones genistein 1, 6-hydroxygenistein 2, 3'-O-methylorobol 3, pratensein 4, biochanin A 8, the flavones 6-hydroxyapigenin 7 and luteolin 5, the flavonol kaempferol 6, as well as the phenolic compound p-coumaric acid 9 reduce significantly the pain at a concentration dose of 1 mg/kg. The most active compounds were 3 and 8 (86.19% and 75.23%, respectively). The obtained aqueous extracts of R. raetam were also evaluated for their antioxidant activities using two different photometric methods; the results revealed that all extracts exerted very low free radical scavenging activity compared to the ...
Analytica Chimica Acta, 2001
A sensitive and simple method for measuring antioxidant activity was developed, based on photosto... more A sensitive and simple method for measuring antioxidant activity was developed, based on photostoragechemiluminescence generated from photolyzed acridine and strong bases. This method was used to evaluate the antioxidant activity of food additives by measuring the inhibition of chemiluminescence at a gradient of antioxidant concentrations. Adipic, tartaric, citric, ascorbic, malic and fumaric acid were employed as antioxidants. The method is more suitable for lipophilic compounds.
Cancers
Increased rates of reactive oxygen/nitrogen species (ROS/RNS) are involved in almost all cancer t... more Increased rates of reactive oxygen/nitrogen species (ROS/RNS) are involved in almost all cancer types, associated with tumor development and progression, causing damage to biomolecules such as proteins, nucleic acids and membrane lipids, in different biological compartments. We used a human tumor xenograft mouse model to evaluate for the first time in parallel the remodeling of fatty acid moieties in erythrocyte membrane phospholipids and the level of ROS-induced DNA lesions in liver and kidney tissues. Using liquid chromatography tandem mass spectrometry the 5’R and 5’S diastereoisomers of 5’,8-cyclo-2’-deoxyadenosine and 5’,8-cyclo-2’-deoxyguanosine, together with 8-oxo-7,8-dihydro-2’-deoxyadenosine, were determined in mice at young (4- and 5-weeks) and old (17-weeks) ages and compared with control SCID mice without tumor implantation. Tumor-bearing mice showed a higher level of ROS-damaged nucleosides in genomic DNA as the age and tumor progress, compared to controls (1.07–1.53-f...
Molecules
The use of liposomes has been crucial for investigations in biomimetic chemical biology as a memb... more The use of liposomes has been crucial for investigations in biomimetic chemical biology as a membrane model and in medicinal chemistry for drug delivery. Liposomes are made of phospholipids whose biophysical characteristics strongly depend on the type of fatty acid moiety, where natural unsaturated lipids always have the double bond geometry in the cis configuration. The influence of lipid double bond configuration had not been considered so far with respect to the competence of liposomes in delivery. We were interested in evaluating possible changes in the molecular properties induced by the conversion of the double bond from cis to trans geometry. Here we report on the effects of the addition of trans-phospholipids supplied in different amounts to other liposome constituents (cholesterol, neutral phospholipids and cationic surfactants), on the size, ζ-potential and stability of liposomal formulations and on their ability to encapsulate two dyes such as rhodamine B and fluorescein. From a biotechnological point of view, trans-containing liposomes proved to have different characteristics from those containing the cis analogues, and to influence the incorporation and release of the dyes. These results open new perspectives in the use of the unnatural lipid geometry, for the purpose of changing liposome behavior and/or of obtaining molecular interferences, also in view of synergic effects of cell toxicity, especially in antitumoral strategies.
Journal of Photochemistry and Photobiology A: Chemistry
ABSTRACT 6,8-Diphenylimidazopyrazinone derivatives having a substituent R (R=CF3, H, and OMe) at ... more ABSTRACT 6,8-Diphenylimidazopyrazinone derivatives having a substituent R (R=CF3, H, and OMe) at pare position of the 8-phenyl group were synthesized and their chemiluminescent properties were investigated. The chemiluminescence maxima (CLmax) of these compounds were observed to be in the range of 513-553 nm with a bathochromic shift that increased with the electron-withdrawing character of R, contrary to the previously observed substituent effect at the 6-postion. The chemiluminescence efficiencies (phi(CL)) of these imidazopyrazinones were improved by the introduction of a p-substituted phenyl group at the 8-position. The quantitative investigation of the three quantum efficiencies (phi(R), phi(S), and phi(FL)) whose product gives us phi(CL) revealed that the phi(CL) gains made were largely because of the increase in the values of the fluorescence quantum yields of the corresponding light emitters (phi(FL)). The yields of the singlet-excited emitters (phi(S)) during the chemiluminescent reaction were found to be very small (0.015-0.019), suggesting that one cannot construct an efficient imidazopyrazinone-chemiluminescence system that is comparable to the aequorin bioluminescence system only by using the electronic effects of substituents.
Free Radical Research
5',8-Cyclo-2'-deoxyadenosine (cdA) and 5&... more 5',8-Cyclo-2'-deoxyadenosine (cdA) and 5',8-cyclo-2'-deoxyguanosine (cdG) in their two diastereomeric forms, 5'S and 5'R, are tandem lesions produced by the attack of hydroxyl radicals to the purine moieties of DNA. Their formation has been found to challenge the cells' repair machinery, initiating the nucleotide excision repair (NER) for restoring the genome integrity. The involvement of oxidatively induced DNA damage in carcinogenesis and the reduced capacity of some cancer cell lines to repair oxidised DNA base lesions, intrigued us to investigate the implication of these lesions in breast cancer, the most frequently occurring cancer in women. Using liquid chromatography tandem mass spectrometry (LC-MS/MS), we measured the levels of diastereomeric cdA's and cdG's in estrogen receptor-alpha positive (ER-α) MCF-7 and triple negative MDA-MB-231 breast cancer cell lines before and after exposure to two different conditions: ionising radiations and hydrogen peroxide, followed by an interval period to allow DNA repair. An increase at the measured levels of all four lesions, i.e. 5'S-cdA, 5'R-cdA, 5'S-cdG and 5'R-cdG, was observed either after γ-irradiation (5 Gy dose) or hydrogen peroxide treatment (300 μM) compared to the untreated cells (control), independently from the length of the interval period for repair. For comparison reasons, we also measured the levels of 8-oxo-2'-deoxyadenosine (8-oxo-dA), a well-known oxidatively induced DNA damage lesion and base excision repair (BER) substrate. The collected data indicate that MCF-7 and MDA-MB-231 breast cancer cells are highly susceptible to radiation-induced DNA damage, being mainly defective in the repair of these lesions.
Inorganic Chemistry
We report on the synthesis, characterization and optoelectronic properties of the novel trimethyl... more We report on the synthesis, characterization and optoelectronic properties of the novel trimethylsulfonium lead triiodide perovskite, (CH 3) 3 SPbI 3. At room temperature, the air-stable compound adopts a hexagonal crystal structure with a 1D network of face-sharing [PbI 6 ] octahedra along the c axis. UV-Vis reflectance spectroscopy on polycrystalline films revealed a band gap of 2.5 eV in good agreement with first-principles calculations, which show a small separation between direct and indirect band gaps. Electrical resistivity measurements on single crystals indicated that the compound behaves as a semi-insulator. According to multi-temperature single-crystal X-ray diffraction, synchrotron powder X-ray diffraction, Raman spectroscopy and differential scanning calorimetry, two fully reversible structural phase transitions occur at-5 °C and ca.-100 °C with reduction of the unit cell symmetry to monoclinic as temperature decreases. The role of the trimethylsulfonium cation regarding the chemical stability and optoelectronic properties of the new compound is discussed in comparison with APbI 3 (A = Cs, methylammonium, and formamidinium cation) that are most commonly used in perovskite solar cells.
Http Dx Doi Org 10 1080 00032719 2011 649456, Mar 1, 2012
Six well established spectrophotometric assays (Folin-Ciocalteau, DPPH, ABTS •+ , FRAP, CUPRAC an... more Six well established spectrophotometric assays (Folin-Ciocalteau, DPPH, ABTS •+ , FRAP, CUPRAC and the o-diphenols method) have been complementary implemented in order to estimate the total phenolic and o-diphenolic content as well as the free radical scavenging and reducing antioxidant capacities of forty white and ten red wines of different varieties and geographic origin. In white wines, the results were weakly correlated, whereas the results in red wines were strongly correlated, therefore postulating their separate use in order to estimate efficiently the correlation between results of these methods. The results were subjected to the unsupervised PCA pattern recognition method to investigate the possible classification of white wines. PC analysis accomplished to frame the wine samples in clear clusters, when the extracted PCA model was based on the results of all six spectrophotometric assays. The usefulness of implementing assays based on different antioxidant mechanisms is discussed. To our knowledge, this is the first time that the different responses of antioxidants to these spectrophotometric assays classified white wines according to their variety.
Http Dx Doi Org 10 1080 00032719 2011 649461, Apr 13, 2012
Journal für praktische Chemie
Chemistry of Natural Compounds
Photochemistry and Photobiology, 2015
A new family of Ru(II)-based photosensitizers was synthesized and systematically characterized. T... more A new family of Ru(II)-based photosensitizers was synthesized and systematically characterized. The ligands employed to coordinate the ruthenium metal center were the commercially available 2,2'-bipyridine and a pyridine-quinoline hybrid bearing an anthracene moiety. The complexes obtained carry either PF6- or Cl(-) counterions. These counterions determine the complexes' hydrophobic or hydrophilic character, respectively, therefore dictating their solubility in biologically related media. All photosensitizers exhibit characteristic, relatively strong and wide UV-Vis absorption spectral profiles. Their high efficiency in generating cytotoxic singlet oxygen was established (up to ΦΔ ~0.8). Moreover, the interaction of these photosensitizers with double-stranded DNA was studied fluoro- and photospectroscopically and their binding affinities were found to be of the order of 3 × 10(7) M(-1) . All complexes are photocytotoxic to DU145 human prostate cancer cells. The highest light-induced toxicity was conferred by the photosensitizers bearing Cl(-) counterions, probably due to the looser ionic "chaperoning" of Cl(-) , in comparison to PF6-, leading to higher cell internalization.
European Journal of Inorganic Chemistry
Activités anti-radicalaires de l'huile essentielle et des extraits bruts de
Microchemical Journal, 2015
ABSTRACT The antioxidant activity of oils is an important parameter that determines oils'... more ABSTRACT The antioxidant activity of oils is an important parameter that determines oils' nutritional value and quality. To date, the methods for the determination of the antioxidant activity of oils are performed under batch conditions, therefore, they have large turnaround times and are labor-intensive. In this work, we describe the first automated method for the determination of the antioxidant activity of edible oils by resorting to the benefits of Flow Injection Analysis (FIA) and the high sensitivity of chemiluminescence (CL) detection. In the developed method, we used the chemiluminescent reaction of bis(2,4, 6-trichlorophenyl) oxalate with the reactive oxygen species produced by the catalytic decomposition of H2O2 by Mn(II) ions. The antioxidants present in the sample scavenge the reactive oxygen species and inhibit the light reaction. 1-Amino-pyrene was used as fluorophore and 1-methyl-imidazole as base catalyst in the reaction. Method validation and application to real samples showed that the analytical features of the method are very satisfactory in terms of linearity of response-lines of standard antioxidant compounds (R2 = 0.98–0.999), precision (%RSD = 0.85–1.9%) and accuracy (%recovery = 91–109%), suggesting that the developed assay could serve as an efficient tool to the routine screening of antioxidant properties in edible oils.