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Papers by leftheris georgakopoulos
PubMed, Apr 9, 2005
Background: The aim of our study was to detect Ep-CAM mRNA in peripheral blood (PB) and bone marr... more Background: The aim of our study was to detect Ep-CAM mRNA in peripheral blood (PB) and bone marrow (BM) samples of patients with metastatic colorectal cancer, using a novel chromatographic method (molecular strip) for the detection of PCR amplified product and to confirm the results by the standard method of agarose gel electrophoresis. Patients and methods: Thirty patients participated in the present study providing 27 PB and 26 BM samples. PB samples were also obtained from 20 healthy volunteers. PCR products were detected using the molecular strip and the results were confirmed by 1.5% agarose gel electrophoresis. Results: In total 26 out of 27 blood samples (96%) and 19 out of 26 bone marrow samples (73%) were found positive for Ep-CAM expression using both methods, whereas none of the 20 healthy controls was found positive for the above marker. Conclusion: Ep-CAM mRNA is highly expressive in peripheral blood of patients with metastatic colorectal cancer. Its prognostic significance should be evaluated in patients with resectable carcinomas. The new strip detection method is highly specific for the PCR product under investigation, reliable, easy to handle, fast and safe for the user.
Biochemical and Biophysical Research Communications, Dec 1, 1995
Biochemical and Biophysical Research Communications, 1996
Primary hepatocytes respond to the proliferating signals of Hepatocyte Growth Factor (HGF) throug... more Primary hepatocytes respond to the proliferating signals of Hepatocyte Growth Factor (HGF) through activation of the tyrosine kinase activity of the met (p 145) receptor. Addition of dHGF in hepatocyte cultures resulted in receptor phosphorylation which co-precipitated with a phosphorylated protein of 85 kDa. This protein was identified as the regulatory subunit of phosphatidylinositol 3-kinase (PI 3-kinase). Co-precipitation of the PI 3-kinase regulatory subunit with the met receptor was observed only with the phosphorylated receptor. Wortmanin, which specifically inhibits PI 3-kinase, was found to abolish the hepatocyte DNA synthetic response due to stimulation with dHGF. It is suggested that the D-3-phosphorylated inositol phospholipids participate as major regulators in the growth and differentiation factor-initiated cascades, this not being restricted to primary hepatocytes.
Ejc Supplements, Sep 1, 2003
Ejc Supplements, Sep 1, 2003
La presente invention concerne une nouvelle technique de detection de l'antigene 17-1A du can... more La presente invention concerne une nouvelle technique de detection de l'antigene 17-1A du cancer dans le sang peripherique, dans des tissus et dans des prelevements d'urine, de meme que des techniques permettant d'etablir le diagnostic et le pronostic d'un cancer ainsi que la surveillance d'une therapie de lutte contre le cancer.
European Journal of Cancer Supplements, 2003
European Journal of Cancer Supplements, 2003
Anticancer research
The aim of our study was to detect Ep-CAM mRNA in peripheral blood (PB) and bone marrow (BM) samp... more The aim of our study was to detect Ep-CAM mRNA in peripheral blood (PB) and bone marrow (BM) samples of patients with metastatic colorectal cancer, using a novel chromatographic method (molecular strip) for the detection of PCR amplified product and to confirm the results by the standard method of agarose gel electrophoresis. Thirty patients participated in the present study providing 27 PB and 26 BM samples. PB samples were also obtained from 20 healthy volunteers. PCR products were detected using the molecular strip and the results were confirmed by 1.5% agarose gel electrophoresis. In total 26 out of 27 blood samples (96%) and 19 out of 26 bone marrow samples (73%) were found positive for Ep-CAM expression using both methods, whereas none of the 20 healthy controls was found positive for the above marker. Ep-CAM mRNA is highly expressive in peripheral blood of patients with metastatic colorectal cancer. Its prognostic significance should be evaluated in patients with resectable c...
Journal of Hepatology, 2001
Journal of Hepatology, 2002
Conclusions: 1. The intrafamilial spread of I-1BV was probably by vertical transmission among bot... more Conclusions: 1. The intrafamilial spread of I-1BV was probably by vertical transmission among both groups, as shown by HBV sequencing. 2. The asian origin people living in Brazil has the same genotype as in their origin country.
Biochemical and Biophysical Research Communications, 1996
Primary hepatocytes respond to the proliferating signals of Hepatocyte Growth Factor (HGF) throug... more Primary hepatocytes respond to the proliferating signals of Hepatocyte Growth Factor (HGF) through activation of the tyrosine kinase activity of the met (p 145) receptor. Addition of dHGF in hepatocyte cultures resulted in receptor phosphorylation which co-precipitated with a phosphorylated protein of 85 kDa. This protein was identified as the regulatory subunit of phosphatidylinositol 3-kinase (PI 3-kinase). Co-precipitation of the PI 3-kinase regulatory subunit with the met receptor was observed only with the phosphorylated receptor. Wortmanin, which specifically inhibits PI 3-kinase, was found to abolish the hepatocyte DNA synthetic response due to stimulation with dHGF. It is suggested that the D-3-phosphorylated inositol phospholipids participate as major regulators in the growth and differentiation factor-initiated cascades, this not being restricted to primary hepatocytes.
Annals of the New York Academy of Sciences, 2006
In this study, we examined several molecular markers in prostate and breast cancer patients and i... more In this study, we examined several molecular markers in prostate and breast cancer patients and in normal individuals. The markers tested were: variations in the quantity of plasma DNA, glutathione-S-transferase P1 gene (GSTP1), Ras association domain family 1A (RASSF1A), and ataxia telangiectasia mutated (ATM) methylation status in plasma, carcinoembryonic antigen (CEA) and prostate-specific membrane antigen (PSMA) mRNA in peripheral blood mononuclear
PubMed, Apr 9, 2005
Background: The aim of our study was to detect Ep-CAM mRNA in peripheral blood (PB) and bone marr... more Background: The aim of our study was to detect Ep-CAM mRNA in peripheral blood (PB) and bone marrow (BM) samples of patients with metastatic colorectal cancer, using a novel chromatographic method (molecular strip) for the detection of PCR amplified product and to confirm the results by the standard method of agarose gel electrophoresis. Patients and methods: Thirty patients participated in the present study providing 27 PB and 26 BM samples. PB samples were also obtained from 20 healthy volunteers. PCR products were detected using the molecular strip and the results were confirmed by 1.5% agarose gel electrophoresis. Results: In total 26 out of 27 blood samples (96%) and 19 out of 26 bone marrow samples (73%) were found positive for Ep-CAM expression using both methods, whereas none of the 20 healthy controls was found positive for the above marker. Conclusion: Ep-CAM mRNA is highly expressive in peripheral blood of patients with metastatic colorectal cancer. Its prognostic significance should be evaluated in patients with resectable carcinomas. The new strip detection method is highly specific for the PCR product under investigation, reliable, easy to handle, fast and safe for the user.
Biochemical and Biophysical Research Communications, Dec 1, 1995
Biochemical and Biophysical Research Communications, 1996
Primary hepatocytes respond to the proliferating signals of Hepatocyte Growth Factor (HGF) throug... more Primary hepatocytes respond to the proliferating signals of Hepatocyte Growth Factor (HGF) through activation of the tyrosine kinase activity of the met (p 145) receptor. Addition of dHGF in hepatocyte cultures resulted in receptor phosphorylation which co-precipitated with a phosphorylated protein of 85 kDa. This protein was identified as the regulatory subunit of phosphatidylinositol 3-kinase (PI 3-kinase). Co-precipitation of the PI 3-kinase regulatory subunit with the met receptor was observed only with the phosphorylated receptor. Wortmanin, which specifically inhibits PI 3-kinase, was found to abolish the hepatocyte DNA synthetic response due to stimulation with dHGF. It is suggested that the D-3-phosphorylated inositol phospholipids participate as major regulators in the growth and differentiation factor-initiated cascades, this not being restricted to primary hepatocytes.
Ejc Supplements, Sep 1, 2003
Ejc Supplements, Sep 1, 2003
La presente invention concerne une nouvelle technique de detection de l'antigene 17-1A du can... more La presente invention concerne une nouvelle technique de detection de l'antigene 17-1A du cancer dans le sang peripherique, dans des tissus et dans des prelevements d'urine, de meme que des techniques permettant d'etablir le diagnostic et le pronostic d'un cancer ainsi que la surveillance d'une therapie de lutte contre le cancer.
European Journal of Cancer Supplements, 2003
European Journal of Cancer Supplements, 2003
Anticancer research
The aim of our study was to detect Ep-CAM mRNA in peripheral blood (PB) and bone marrow (BM) samp... more The aim of our study was to detect Ep-CAM mRNA in peripheral blood (PB) and bone marrow (BM) samples of patients with metastatic colorectal cancer, using a novel chromatographic method (molecular strip) for the detection of PCR amplified product and to confirm the results by the standard method of agarose gel electrophoresis. Thirty patients participated in the present study providing 27 PB and 26 BM samples. PB samples were also obtained from 20 healthy volunteers. PCR products were detected using the molecular strip and the results were confirmed by 1.5% agarose gel electrophoresis. In total 26 out of 27 blood samples (96%) and 19 out of 26 bone marrow samples (73%) were found positive for Ep-CAM expression using both methods, whereas none of the 20 healthy controls was found positive for the above marker. Ep-CAM mRNA is highly expressive in peripheral blood of patients with metastatic colorectal cancer. Its prognostic significance should be evaluated in patients with resectable c...
Journal of Hepatology, 2001
Journal of Hepatology, 2002
Conclusions: 1. The intrafamilial spread of I-1BV was probably by vertical transmission among bot... more Conclusions: 1. The intrafamilial spread of I-1BV was probably by vertical transmission among both groups, as shown by HBV sequencing. 2. The asian origin people living in Brazil has the same genotype as in their origin country.
Biochemical and Biophysical Research Communications, 1996
Primary hepatocytes respond to the proliferating signals of Hepatocyte Growth Factor (HGF) throug... more Primary hepatocytes respond to the proliferating signals of Hepatocyte Growth Factor (HGF) through activation of the tyrosine kinase activity of the met (p 145) receptor. Addition of dHGF in hepatocyte cultures resulted in receptor phosphorylation which co-precipitated with a phosphorylated protein of 85 kDa. This protein was identified as the regulatory subunit of phosphatidylinositol 3-kinase (PI 3-kinase). Co-precipitation of the PI 3-kinase regulatory subunit with the met receptor was observed only with the phosphorylated receptor. Wortmanin, which specifically inhibits PI 3-kinase, was found to abolish the hepatocyte DNA synthetic response due to stimulation with dHGF. It is suggested that the D-3-phosphorylated inositol phospholipids participate as major regulators in the growth and differentiation factor-initiated cascades, this not being restricted to primary hepatocytes.
Annals of the New York Academy of Sciences, 2006
In this study, we examined several molecular markers in prostate and breast cancer patients and i... more In this study, we examined several molecular markers in prostate and breast cancer patients and in normal individuals. The markers tested were: variations in the quantity of plasma DNA, glutathione-S-transferase P1 gene (GSTP1), Ras association domain family 1A (RASSF1A), and ataxia telangiectasia mutated (ATM) methylation status in plasma, carcinoembryonic antigen (CEA) and prostate-specific membrane antigen (PSMA) mRNA in peripheral blood mononuclear