lekha saha - Academia.edu (original) (raw)

Papers by lekha saha

Trials, Aug 17, 2021

Objectives: To compare the efficacy of chlorhexidine-alcohol and povidone-iodine as preoperative ... more Objectives: To compare the efficacy of chlorhexidine-alcohol and povidone-iodine as preoperative antiseptic skin preparation for prevention of surgical site infection (SSI) after cesarean delivery (CD). Materials and methods: A total of 311 eligible women who underwent CS were recruited in the study after fulfilling all the eligibility and exclusion criteria. Patients were randomized into two groups (153 in chlorhexidinealcohol group and 158 in povidone-iodine group) by a computer-generated randomization table. Patients were followed for a period of 30 days in postoperative period to monitor for SSI. Results: The rate of SSI in the chlorhexidine-alcohol group is 5.4% and that of the povidone-iodine group is 8.6%. E. coli, K. pneumoniae, and Acinetobacter baumannii were the most common organisms isolated. E. coli was found in 9.5% of the total SSI cases. Conclusions: The study found that the patients who received chlorhexidine-alcohol as skin antiseptic had less chance of developing SSI than those who received povidone-iodine; however, it did not reach a statistical significance.

The coexistence of autism spectrum disorder (ASD) and epilepsy is very much prevalent in certain ... more The coexistence of autism spectrum disorder (ASD) and epilepsy is very much prevalent in certain neurodevelopment disorders and their association could be best understood by examining shared phenotypes, pathophysiological mechanisms and risk factors involved. Various genetic as well as environmental factors affecting the role of certain biological pathways in synapse formation and its maintenance along with cellular growth factors and regulatory proteins have been found to be responsible for the complexities involved in co-occurrence disorders. In this review, we have briefly described potential in vivo as well as in vitro models that have helped us in validating certain associated risk factors and decipher the bases of mechanism in developing both disorders, thus further helpful in finding useful target-specific therapy for patients.

PubMed, 1999

The effect of ondansetron was investigated in wrap-restraint and conditioned emotional (footshock... more The effect of ondansetron was investigated in wrap-restraint and conditioned emotional (footshock) stress induced colonic hypermotility in rat. Ondansetron was administered in doses of 0.1, 0.5 and 1 mg/kg, sc. In wrap-restraint group, ondansetron dose-dependently reduced the fecal output to baseline levels (0.51 ± 0.20 gm/rat, 0.48 ± 0.38 gm/rat, 0.38 ± 0.16 gm/rat with 0.1, 0.5 and 1 mg/kg respectively). Diazepam, 5 mg/kg and atropine, 1 mg/kg also decreased the fecal output in this model. In footshock group, ordansetron did not affect the emotional stress induced increase in fecal pellet output. Diazepam and atropine however, decreased the fecal output in this model also.

PubMed, Oct 1, 2000

Nimodipine, a dihydropyridine calcium channel blocker, was administered orally using two differen... more Nimodipine, a dihydropyridine calcium channel blocker, was administered orally using two different doses (40 mg and 60 mg/kg/day) to rats. Both short term (2 weeks) and long term (6 weeks) effects of the drug were observed. The drug administration resulted in a marked decrease in sperm density, sperm motility and acrozomal reaction. Zona-pellucida penetration by the sperm obtained from drug-treated animals was significantly lower when compared with sperm from normal animals. Nimodipine stimulated Ca2+ ATPase activity in isolated plasma membrane of rate spermatozoa. In conclusion, short term and long term administration of nimodipine has deleterious effect on male reproductive functions in rats.

Methods and Findings in Experimental and Clinical Pharmacology, 2000

Annals of Hematology, Jul 13, 2023

$Research paper thumbnail of Relative refractoriness of breast cancer cells to tumour necrosis factor‐α induced necroptosis$

Clinical and Experimental Pharmacology and Physiology, Aug 29, 2022

Pharmacoepidemiology and Drug Safety, 2000

Ojective- Pattern of drug use in an ambulatory paediatric patients in a tertiary care hospital in... more Ojective- Pattern of drug use in an ambulatory paediatric patients in a tertiary care hospital in Northern India was studied.Methods- Prescribing patterns were studied prospectively, using drug use indicators developed by WHO/International Network for Rational Utilization of Drugs (INRUD).Results- The most frequent diagnosis was seizure disorder (26.9%) and antiepileptics were the most often prescribed drugs (24.6); followed by vitamin and mineral supplements (20.1%), and cough/cold preparations (16%). Use of vitamin and mineral supplements was disproportionately high (20.1%) when compared to the number of cases of anaemia (2.3% and malnutrition (2.6%). The average number of drugs per encounter (1.32), percentage of encounters with an antibiotic (6.1%) or injection (0.2%) prescribed, were all within the acceptable range. The percentage of drugs prescribed by generic name was too low, at 11.2%.Conclusion- Barring the frequent use of supplements and low rate of generic prescribing, prescribing trends were found to be rational in other aspects in the study population. Copyright (c) 2000 John Wiley &amp;amp; Sons, Ltd.

World Journal of Clinical Cases

World Journal of Gastrointestinal Surgery, Apr 27, 2023

Bollettino chimico farmaceutico

Antinociceptive effect of imipramine and morphine was studied in rats using tail flick method. Mo... more Antinociceptive effect of imipramine and morphine was studied in rats using tail flick method. Morphine and imipramine both increased the latency of tail flick response. Pretreatment with naloxone (0.5 mg kg-1) decreased the antinociceptive effect of morphine (1 mg kg-1) and imipramine (10 mg kg-1). In order to see if antinociceptive effect of morphine or imipramine involve cholinergic mechanisms, mecamylamine (1 mg kg-1) or atropine (1 mg kg-1) were administered 30 minutes before morphine (1 mg kg-1) or imipramine (10 mg kg-1) administration. Mecamylamine decreased the antinociceptive effect of morphine and imipramine (P < 0.001), whereas atropine had no effect. Thus, opiodergic and nicotinic systems appear to be involved in morphine and imipramine induced antinociception.

World Journal of Gastroenterology

Colorectal cancer (CRC) affects one in every 23 men and 25 women, making it the third most common... more Colorectal cancer (CRC) affects one in every 23 men and 25 women, making it the third most common cancer. CRC accounts for 8% of all cancer-related deaths, making it the second most common cause of death due to cancer. Standard and conventional CRC treatments include surgical expurgation for resectable CRC and radiotherapy, chemotherapy, immunotherapy, and their combinational regimen for non-resectable CRC. Despite these tactics, nearly half of patients develop incurable recurring CRC. These constraints necessitate the development of new target-specific therapeutic strategies such as targeted immune boosting therapies, non-coding RNAs based therapies, natural products, oncolytic viral therapies, have shown promising results in preclinical and clinical studies. We have tethered the entire evolutionary trends in the development of CRC treatments and have gone over the potential of new therapies and how they might be used in conjunction with conventional treatments, as well as their ad...

Cell Biochemistry and Function

Methods and findings in experimental and clinical pharmacology, 2000

Angiotensin-converting enzyme is present in the male reproductive system but its role in the phys... more Angiotensin-converting enzyme is present in the male reproductive system but its role in the physiology of reproduction is not known. To see the effect of angiotensin-converting enzyme on spermatozoal functions, lisinopril, an angiotensin-converting enzyme inhibitor, was administered orally using two different doses (10 and 20 mg/kg/day) to rats. Both short-term (2 weeks) and long-term (6 weeks) effects of the drug were observed. Lisinopril treatment resulted in a marked decrease in sperm density, sperm motility and zona pellucida penetration. Acrosome reaction by spermatozoa obtained from drug-treated animals was significantly lower when compared with spermatozoa from normal animals.

Indian journal of physiology and pharmacology, 2001

Journal of Basic and Clinical Physiology and Pharmacology

Objectives Aspirin is an anti-inflammatory drug commonly used as an analgesic and in cardiovascul... more Objectives Aspirin is an anti-inflammatory drug commonly used as an analgesic and in cardiovascular disorders. However, many studies have highlighted its anti-cancer properties, especially in colorectal, lung, head and neck, and breast cancers. In this work, we tried to study the effect of aspirin on the TNF-α-mediated cell survival and death pathways in two cell lines representing two different subtypes of breast cancer. TNF-α-mediated stimulation of a cell can result in its proliferation via the NF-κB pathway or its death via either apoptosis or a programmed form of necrosis called necroptosis. The latter is believed to come into the picture only when apoptosis is inhibited. Methods In this work, we studied the effect of aspirin on the TNF-α-mediated cell survival pathway and observed a decrease in expression of the NF-κB pathway regulators, its nuclear translocation, and phosphorylation in a dose-dependent manner. The effect of aspirin on the TNF-α-mediated cell death showed sign...