syazana lim - Academia.edu (original) (raw)
Papers by syazana lim
Encyclopedia of Green Materials, 2022
Food Chemistry, Sep 1, 2016
A label free electrochemical immunosensor for sensitive detection of porcine serum albumin (PSA) ... more A label free electrochemical immunosensor for sensitive detection of porcine serum albumin (PSA) is reported in this work. The immunosensor was constructed by first electrochemically reducing 4-carboxyphenyl diazonium salt, which had been electrochemically generated in situ, to a stable 4-carboxyphenyl layer on carbon nanofiber-modified screen printed electrode. Antibodies were covalently attached onto the electrode using carbodiimide chemistry between the carboxylic groups of the 4-carboxyphenyl layer and amine groups of the antibody. Using the strong affinities of serum albumins towards anions, the increase in cathodic peak current in anionic redox probe after immunocomplex formation with antibodies was used for PSA detection. The reported immunosensor demonstrated a linear range from 0.5 to 500pg/mL for the measurement of PSA with detection limit of 0.5pg/mL in buffer solution. Cross-reactivity studies have shown excellent specificity with satisfactory recovery of PSA in fresh meat samples without the need of sample dilution.
The Royal Society of Chemistry eBooks, Oct 12, 2016
This chapter is divided into sections introducing the principal concept of a biosensor and descri... more This chapter is divided into sections introducing the principal concept of a biosensor and describing the different key elements in its construction. A discussion of various natural and synthetic receptors used in molecular biorecognition, their interactions with analytes and limitations are included. This chapter is also devoted in providing readers an overview of different configurations of transducer, advantages and drawbacks of each method. As a conclusion, an emerging trend of biosensors as a useful analytical tool will be evaluated.
Advances in finance, accounting, and economics book series, Apr 14, 2023
Encyclopedia of Green Materials, 2022
Encyclopedia of Green Materials, 2022
COVID-19 and the Sustainable Development Goals
Journal of the Science of Food and Agriculture
SSRN Electronic Journal, 2022
Journal of Food Processing and Preservation
Biosensors & bioelectronics, 2018
In this work, a nanocomposite of gold nanoparticles (AuNPs), carbon nano-onions (CNOs), single-wa... more In this work, a nanocomposite of gold nanoparticles (AuNPs), carbon nano-onions (CNOs), single-walled carbon nanotubes (SWCNTs) and chitosan (CS) (AuNPs/CNOs/SWCNTs/CS) was prepared for the development of highly sensitive electrochemical immunosensor for the detection of carcinoembryonic antigen (CEA), clinical tumor marker. Firstly, layer-by-layer fabrication of the CEA-immunosensors was studied using cyclic voltammetry (CV) and square wave voltammetry (SWV). By combining the advantages of large surface area and electronic properties of AuNPs, CNOs, SWCNTs, and film forming properties of CS, AuNPs/CNOs/SWCNTs/CS-nanocomposite-modified glassy carbon electrode showed a 200% increase in effective surface area and electronic conductivity. The calibration plot gave a negative linear relationship between log[concentration] of CEA and electrical current with a correlation coefficient of 0.9875. The CEA-immunosensor demonstrated a wide linear detection range of 100 fg mL to 400 ng mL with ...
Food Biosensors
This chapter is divided into sections introducing the principal concept of a biosensor and descri... more This chapter is divided into sections introducing the principal concept of a biosensor and describing the different key elements in its construction. A discussion of various natural and synthetic receptors used in molecular biorecognition, their interactions with analytes and limitations are included. This chapter is also devoted in providing readers an overview of different configurations of transducer, advantages and drawbacks of each method. As a conclusion, an emerging trend of biosensors as a useful analytical tool will be evaluated.
Food Chemistry, 2016
A label free electrochemical immunosensor for sensitive detection of porcine serum albumin (PSA) ... more A label free electrochemical immunosensor for sensitive detection of porcine serum albumin (PSA) is reported in this work. The immunosensor was constructed by first electrochemically reducing 4-carboxyphenyl diazonium salt, which had been electrochemically generated in situ, to a stable 4-carboxyphenyl layer on carbon nanofiber-modified screen printed electrode. Antibodies were covalently attached onto the electrode using carbodiimide chemistry between the carboxylic groups of the 4-carboxyphenyl layer and amine groups of the antibody. Using the strong affinities of serum albumins towards anions, the increase in cathodic peak current in anionic redox probe after immunocomplex formation with antibodies was used for PSA detection. The reported immunosensor demonstrated a linear range from 0.5 to 500pg/mL for the measurement of PSA with detection limit of 0.5pg/mL in buffer solution. Cross-reactivity studies have shown excellent specificity with satisfactory recovery of PSA in fresh meat samples without the need of sample dilution.
RSC Adv., 2014
This study describes a highly sensitive electrochemical immunosensor for the detection of human c... more This study describes a highly sensitive electrochemical immunosensor for the detection of human chorionic gonadotropin (hCG) that uses gold nanoparticles (AuNP) as the electrochemical label and graphene as electrode material.
RSC Advances, 2016
In recent years, tremendous advances have been made in biosensors based on nanoscale electrochemi... more In recent years, tremendous advances have been made in biosensors based on nanoscale electrochemical immunosensors for use in the fields of agriculture, food safety, biomedicine, quality control, and environmental and industrial monitoring.
Encyclopedia of Green Materials, 2022
Food Chemistry, Sep 1, 2016
A label free electrochemical immunosensor for sensitive detection of porcine serum albumin (PSA) ... more A label free electrochemical immunosensor for sensitive detection of porcine serum albumin (PSA) is reported in this work. The immunosensor was constructed by first electrochemically reducing 4-carboxyphenyl diazonium salt, which had been electrochemically generated in situ, to a stable 4-carboxyphenyl layer on carbon nanofiber-modified screen printed electrode. Antibodies were covalently attached onto the electrode using carbodiimide chemistry between the carboxylic groups of the 4-carboxyphenyl layer and amine groups of the antibody. Using the strong affinities of serum albumins towards anions, the increase in cathodic peak current in anionic redox probe after immunocomplex formation with antibodies was used for PSA detection. The reported immunosensor demonstrated a linear range from 0.5 to 500pg/mL for the measurement of PSA with detection limit of 0.5pg/mL in buffer solution. Cross-reactivity studies have shown excellent specificity with satisfactory recovery of PSA in fresh meat samples without the need of sample dilution.
The Royal Society of Chemistry eBooks, Oct 12, 2016
This chapter is divided into sections introducing the principal concept of a biosensor and descri... more This chapter is divided into sections introducing the principal concept of a biosensor and describing the different key elements in its construction. A discussion of various natural and synthetic receptors used in molecular biorecognition, their interactions with analytes and limitations are included. This chapter is also devoted in providing readers an overview of different configurations of transducer, advantages and drawbacks of each method. As a conclusion, an emerging trend of biosensors as a useful analytical tool will be evaluated.
Advances in finance, accounting, and economics book series, Apr 14, 2023
Encyclopedia of Green Materials, 2022
Encyclopedia of Green Materials, 2022
COVID-19 and the Sustainable Development Goals
Journal of the Science of Food and Agriculture
SSRN Electronic Journal, 2022
Journal of Food Processing and Preservation
Biosensors & bioelectronics, 2018
In this work, a nanocomposite of gold nanoparticles (AuNPs), carbon nano-onions (CNOs), single-wa... more In this work, a nanocomposite of gold nanoparticles (AuNPs), carbon nano-onions (CNOs), single-walled carbon nanotubes (SWCNTs) and chitosan (CS) (AuNPs/CNOs/SWCNTs/CS) was prepared for the development of highly sensitive electrochemical immunosensor for the detection of carcinoembryonic antigen (CEA), clinical tumor marker. Firstly, layer-by-layer fabrication of the CEA-immunosensors was studied using cyclic voltammetry (CV) and square wave voltammetry (SWV). By combining the advantages of large surface area and electronic properties of AuNPs, CNOs, SWCNTs, and film forming properties of CS, AuNPs/CNOs/SWCNTs/CS-nanocomposite-modified glassy carbon electrode showed a 200% increase in effective surface area and electronic conductivity. The calibration plot gave a negative linear relationship between log[concentration] of CEA and electrical current with a correlation coefficient of 0.9875. The CEA-immunosensor demonstrated a wide linear detection range of 100 fg mL to 400 ng mL with ...
Food Biosensors
This chapter is divided into sections introducing the principal concept of a biosensor and descri... more This chapter is divided into sections introducing the principal concept of a biosensor and describing the different key elements in its construction. A discussion of various natural and synthetic receptors used in molecular biorecognition, their interactions with analytes and limitations are included. This chapter is also devoted in providing readers an overview of different configurations of transducer, advantages and drawbacks of each method. As a conclusion, an emerging trend of biosensors as a useful analytical tool will be evaluated.
Food Chemistry, 2016
A label free electrochemical immunosensor for sensitive detection of porcine serum albumin (PSA) ... more A label free electrochemical immunosensor for sensitive detection of porcine serum albumin (PSA) is reported in this work. The immunosensor was constructed by first electrochemically reducing 4-carboxyphenyl diazonium salt, which had been electrochemically generated in situ, to a stable 4-carboxyphenyl layer on carbon nanofiber-modified screen printed electrode. Antibodies were covalently attached onto the electrode using carbodiimide chemistry between the carboxylic groups of the 4-carboxyphenyl layer and amine groups of the antibody. Using the strong affinities of serum albumins towards anions, the increase in cathodic peak current in anionic redox probe after immunocomplex formation with antibodies was used for PSA detection. The reported immunosensor demonstrated a linear range from 0.5 to 500pg/mL for the measurement of PSA with detection limit of 0.5pg/mL in buffer solution. Cross-reactivity studies have shown excellent specificity with satisfactory recovery of PSA in fresh meat samples without the need of sample dilution.
RSC Adv., 2014
This study describes a highly sensitive electrochemical immunosensor for the detection of human c... more This study describes a highly sensitive electrochemical immunosensor for the detection of human chorionic gonadotropin (hCG) that uses gold nanoparticles (AuNP) as the electrochemical label and graphene as electrode material.
RSC Advances, 2016
In recent years, tremendous advances have been made in biosensors based on nanoscale electrochemi... more In recent years, tremendous advances have been made in biosensors based on nanoscale electrochemical immunosensors for use in the fields of agriculture, food safety, biomedicine, quality control, and environmental and industrial monitoring.