lemya kaddam - Academia.edu (original) (raw)

Papers by lemya kaddam

Research paper thumbnail of The association between shistosomiasis and enteric fever in a single Schistosoma endemic area in Sudan

International Journal of Pharmaceutical Sciences and Research, 2014

Research paper thumbnail of Detection of NDM and OXA-48 Resistant Genes in Acinetobacter Baumannii Isolated FromIntensive Care Units’ Patients Clinical Samples in Khartoum State

Background: Acinetobacter baumannii is an opportunistic bacterial pathogen with intrinsic and acq... more Background: Acinetobacter baumannii is an opportunistic bacterial pathogen with intrinsic and acquired resistance to many antibiotics causing high rates of morbidity and mortality. This study was aimed to detect MDR Acinetobacter baumannii and its resistant genes (blaNDM, blaOXA48) from clinical isolates in Khartoum state. Method: A cross sectional hospital-based study was done during the period fromApril to July 2019. A total of 50 clinical isolates were obtained from samples of patients in intensive care units (ICUs) for the purpose of molecular confirming of A. baumannii and detecting NDM and OXA-48 resistance genes by usingconventional PCR. Results: Out of 50 isolates investigated PCR was confirmed 47 (94%) as A. baumannii isolates , while 3(6%) isolates were appeared to be other species. Moreover,the 47 A. baumannii isolates were examined for the presences of resistant genes and the result showed that NDM gene was detected in 2 isolates (4.3%) and OXA-48 gene was detected in on...

Research paper thumbnail of Immunological and Molecular Detection of Chlamydia Trachomatis Among Women at Reproductive Age Attending Omdurman Maternity Hospital

ABSTRACT: Chlamydia trachomatis is most common bacterial sexually transmitted disease, Over 100 m... more ABSTRACT: Chlamydia trachomatis is most common bacterial sexually transmitted disease, Over 100 million men and women worldwide are infected with Chlamydia at any point in time. The aim of this study to detect the presence of C. trachomatis antibodies and antigen among women on the reproductive age and to study the relationship between the presence of C. trachomatis and age, abortion and ectopic pregnancy. Five hundered women classify into three hundred pregnant women with a gestational age between 12 and 36 weeks and two hundred non pregnant women. Two blood samples collected individually in two different containers, one EDTA container for PCR technique and plane container for ELISA testing during the period June 2011 to August 2013. Thirty one samples (6.2%) were positive for C. trachomtis IgG only, while 8 samples (1.6%) were positive for both IgM and IgG. Out of 31 samples 25 samples (5%) were positive by using PCR technique. The results revealed

Research paper thumbnail of Detection of NDM and OXA-48 Resistant Genes in Acinetobacter Baumannii Isolated FromIntensive Care Units’ Patients Clinical Samples in Khartoum State

Background: Acinetobacter baumannii is an opportunistic bacterial pathogen with intrinsic and acq... more Background: Acinetobacter baumannii is an opportunistic bacterial pathogen with intrinsic and acquired resistance to many antibiotics causing high rates of morbidity and mortality. This study was aimed to detect MDR Acinetobacter baumannii and its resistant genes (blaNDM, blaOXA48) from clinical isolates in Khartoum state. Method: A cross sectional hospital-based study was done during the period fromApril to July 2019. A total of 50 clinical isolates were obtained from samples of patients in intensive care units (ICUs) for the purpose of molecular confirming of A. baumannii and detecting NDM and OXA-48 resistance genes by usingconventional PCR. Results: Out of 50 isolates investigated PCR was confirmed 47 (94%) as A. baumannii isolates , while 3(6%) isolates were appeared to be other species. Moreover,the 47 A. baumannii isolates were examined for the presences of resistant genes and the result showed that NDM gene was detected in 2 isolates (4.3%) and OXA-48 gene was detected in on...

Research paper thumbnail of External ocular bacterial infections among Sudanese children at Khartoum State, Sudan

African Journal of Microbiology Research, 2016

Ocular infections are widespread and they exert heavy burden on eye health. Virtually, any eye co... more Ocular infections are widespread and they exert heavy burden on eye health. Virtually, any eye component can be infected by a diversity of bacteria. The present study was performed to determine the prevalence of external ocular bacterial infections and to find out antibiotic susceptibility pattern of bacterial isolates at eye care hospitals in Khartoum, Sudan. Two hundred and four corneal scrape and drained pus materials were received from infected eyes with clinical diagnoses of bacterial conjunctivitis, keratoconjunctivitis, keratitis, blepharo-conjunctivitis, blepharitis, dacryo-cystitis and eye abscess. Culture, microscopy with Gram's stain of both samples, bacterial colonies and biochemical tests were carried out. Antibiotic susceptibility analysis using Kirby-Bauer disc diffusion test and standard table of antibiotic susceptibility was performed. Out of 204 samples processed, 130 (63.7%) yielded bacterial growth. The most prevalent bacterial eye infection was conjunctivitis (59.2%). Of all the isolates, 75 (57.7%) were Gram's positive and 55 (42.3%) were Gram's negative. Coagulase positive Staphylococcus aureus were the most prevalent, 39 (30%) followed by Streptococcus pneumonia 31 (23.8%), Haemophilus influenzae 22 (16.9%), Pseudomonas aeruginosa 13 (10%) and Neisseria gonorrheae 10 (7.7%). Gram positive bacteria were highly sensitive to vancomycin (95%), followed by chloramphenicol and ciprofloxacin (91%) and ceftriaxone (84%), while the majority were resistant to penicillin (72%). Gram-negative organisms were highly susceptible to amikacin (92.7%) followed by ceftriaxone (87.3%) and ciprofloxacin (78.2%). Major resistance was towards cotrimoxazole (82%) and ampicillin (73%).

Research paper thumbnail of Detection of Chlamydia trachomatis infection and its association with ectopic pregnancy among pregnant ladies attending Omderman maternity hospital: A case study

Chlamydia trachomatis infection is a worldwide-distributed sexually transmitted infection. Rapid ... more Chlamydia trachomatis infection is a worldwide-distributed sexually transmitted infection. Rapid detection of Chlamydial infections in the medical laboratory is very important. The aim of the presence study is to detect IgG and IgM in serum of pregnant women, and to detect the presence of C .trachomatis antibodies in women with ectopic pregnancy. Three hundred blood samples were collected from pregnant women with a gestational age between 25 and 36 weeks. Anti- Chlamydia IgG and anti- Chlamydia IgM were detected using ELISA technique. The result revealed that the rate of anti Chlamydia IgG among pregnant women was 17 (5.6%) and 4 (1.3%) were positive for both anti- Chlamydia IgM and IgG. This study showed a correlation between C. trachomatis positivity and ectopic pregnancy. Screening program of C.trachomatis is very importance in the prevention of long term problems.

Research paper thumbnail of The association between shistosomiasis and enteric fever in a single Schistosoma endemic area in Sudan

International Journal of Pharmaceutical Sciences and Research, 2014

Research paper thumbnail of Detection of NDM and OXA-48 Resistant Genes in Acinetobacter Baumannii Isolated FromIntensive Care Units’ Patients Clinical Samples in Khartoum State

Background: Acinetobacter baumannii is an opportunistic bacterial pathogen with intrinsic and acq... more Background: Acinetobacter baumannii is an opportunistic bacterial pathogen with intrinsic and acquired resistance to many antibiotics causing high rates of morbidity and mortality. This study was aimed to detect MDR Acinetobacter baumannii and its resistant genes (blaNDM, blaOXA48) from clinical isolates in Khartoum state. Method: A cross sectional hospital-based study was done during the period fromApril to July 2019. A total of 50 clinical isolates were obtained from samples of patients in intensive care units (ICUs) for the purpose of molecular confirming of A. baumannii and detecting NDM and OXA-48 resistance genes by usingconventional PCR. Results: Out of 50 isolates investigated PCR was confirmed 47 (94%) as A. baumannii isolates , while 3(6%) isolates were appeared to be other species. Moreover,the 47 A. baumannii isolates were examined for the presences of resistant genes and the result showed that NDM gene was detected in 2 isolates (4.3%) and OXA-48 gene was detected in on...

Research paper thumbnail of Immunological and Molecular Detection of Chlamydia Trachomatis Among Women at Reproductive Age Attending Omdurman Maternity Hospital

ABSTRACT: Chlamydia trachomatis is most common bacterial sexually transmitted disease, Over 100 m... more ABSTRACT: Chlamydia trachomatis is most common bacterial sexually transmitted disease, Over 100 million men and women worldwide are infected with Chlamydia at any point in time. The aim of this study to detect the presence of C. trachomatis antibodies and antigen among women on the reproductive age and to study the relationship between the presence of C. trachomatis and age, abortion and ectopic pregnancy. Five hundered women classify into three hundred pregnant women with a gestational age between 12 and 36 weeks and two hundred non pregnant women. Two blood samples collected individually in two different containers, one EDTA container for PCR technique and plane container for ELISA testing during the period June 2011 to August 2013. Thirty one samples (6.2%) were positive for C. trachomtis IgG only, while 8 samples (1.6%) were positive for both IgM and IgG. Out of 31 samples 25 samples (5%) were positive by using PCR technique. The results revealed

Research paper thumbnail of Detection of NDM and OXA-48 Resistant Genes in Acinetobacter Baumannii Isolated FromIntensive Care Units’ Patients Clinical Samples in Khartoum State

Background: Acinetobacter baumannii is an opportunistic bacterial pathogen with intrinsic and acq... more Background: Acinetobacter baumannii is an opportunistic bacterial pathogen with intrinsic and acquired resistance to many antibiotics causing high rates of morbidity and mortality. This study was aimed to detect MDR Acinetobacter baumannii and its resistant genes (blaNDM, blaOXA48) from clinical isolates in Khartoum state. Method: A cross sectional hospital-based study was done during the period fromApril to July 2019. A total of 50 clinical isolates were obtained from samples of patients in intensive care units (ICUs) for the purpose of molecular confirming of A. baumannii and detecting NDM and OXA-48 resistance genes by usingconventional PCR. Results: Out of 50 isolates investigated PCR was confirmed 47 (94%) as A. baumannii isolates , while 3(6%) isolates were appeared to be other species. Moreover,the 47 A. baumannii isolates were examined for the presences of resistant genes and the result showed that NDM gene was detected in 2 isolates (4.3%) and OXA-48 gene was detected in on...

Research paper thumbnail of External ocular bacterial infections among Sudanese children at Khartoum State, Sudan

African Journal of Microbiology Research, 2016

Ocular infections are widespread and they exert heavy burden on eye health. Virtually, any eye co... more Ocular infections are widespread and they exert heavy burden on eye health. Virtually, any eye component can be infected by a diversity of bacteria. The present study was performed to determine the prevalence of external ocular bacterial infections and to find out antibiotic susceptibility pattern of bacterial isolates at eye care hospitals in Khartoum, Sudan. Two hundred and four corneal scrape and drained pus materials were received from infected eyes with clinical diagnoses of bacterial conjunctivitis, keratoconjunctivitis, keratitis, blepharo-conjunctivitis, blepharitis, dacryo-cystitis and eye abscess. Culture, microscopy with Gram's stain of both samples, bacterial colonies and biochemical tests were carried out. Antibiotic susceptibility analysis using Kirby-Bauer disc diffusion test and standard table of antibiotic susceptibility was performed. Out of 204 samples processed, 130 (63.7%) yielded bacterial growth. The most prevalent bacterial eye infection was conjunctivitis (59.2%). Of all the isolates, 75 (57.7%) were Gram's positive and 55 (42.3%) were Gram's negative. Coagulase positive Staphylococcus aureus were the most prevalent, 39 (30%) followed by Streptococcus pneumonia 31 (23.8%), Haemophilus influenzae 22 (16.9%), Pseudomonas aeruginosa 13 (10%) and Neisseria gonorrheae 10 (7.7%). Gram positive bacteria were highly sensitive to vancomycin (95%), followed by chloramphenicol and ciprofloxacin (91%) and ceftriaxone (84%), while the majority were resistant to penicillin (72%). Gram-negative organisms were highly susceptible to amikacin (92.7%) followed by ceftriaxone (87.3%) and ciprofloxacin (78.2%). Major resistance was towards cotrimoxazole (82%) and ampicillin (73%).

Research paper thumbnail of Detection of Chlamydia trachomatis infection and its association with ectopic pregnancy among pregnant ladies attending Omderman maternity hospital: A case study

Chlamydia trachomatis infection is a worldwide-distributed sexually transmitted infection. Rapid ... more Chlamydia trachomatis infection is a worldwide-distributed sexually transmitted infection. Rapid detection of Chlamydial infections in the medical laboratory is very important. The aim of the presence study is to detect IgG and IgM in serum of pregnant women, and to detect the presence of C .trachomatis antibodies in women with ectopic pregnancy. Three hundred blood samples were collected from pregnant women with a gestational age between 25 and 36 weeks. Anti- Chlamydia IgG and anti- Chlamydia IgM were detected using ELISA technique. The result revealed that the rate of anti Chlamydia IgG among pregnant women was 17 (5.6%) and 4 (1.3%) were positive for both anti- Chlamydia IgM and IgG. This study showed a correlation between C. trachomatis positivity and ectopic pregnancy. Screening program of C.trachomatis is very importance in the prevention of long term problems.