luis amendola - Academia.edu (original) (raw)

Papers by luis amendola

Research paper thumbnail of Clinicopathologic profile of breast cancer patients treated with neoadjuvant chemotherapy at HUCFF/UFRJ

Mastology, 2021

Introduction: The objective of this study is to describe the profile of patients from a public in... more Introduction: The objective of this study is to describe the profile of patients from a public institution, submitted to neoadjuvant chemotherapy (NACT), comparing the verified pathological response with literature data. Methods: Observational retrospective cohort study on breast cancer patients diagnosed between September 2001 and October 2018 and treated with NACT at Hospital Universitário Clementino Fraga Filho (HUCFF/UFRJ), located in Rio de Janeiro, Brazil. The adopted neoadjuvant chemotherapy regimen was based on anthracycline and docetaxel. Results: A total of 133 patients were evaluated. The average age in this group was 54 years (28-86), 49 women (37%) were under 50 years old. The following distribution by molecular subtype was observed: overexpression or amplification of the human epidermal growth factor receptor 2 (HER2+) (13 women, 26.6%), Luminal (19 women, 38.8%), and Triple-negative (TN) (17 women, 34.6%). The HER2+ and TN subtypes had a higher incidence of cases betw...

Research paper thumbnail of Prevalence of BRCA1 and BRCA2 gene mutations in families with medium and high risk of breast and ovarian cancer in Brazil

Brazilian Journal of Medical and Biological Research, 2009

Research paper thumbnail of Prevalence of BRCA1 and BRCA2 genes mutations in a sample of families with high risk of breast and ovarian cancer in Brazil

Cancer Research, 2009

Abstract #6020 Introduction: Of all malignant neoplasia among women, breast cancer has the highes... more Abstract #6020 Introduction: Of all malignant neoplasia among women, breast cancer has the highest incidence rate in Brazil. The objective of the present study was to ascertain the frequency of genetic modifications in a high risk population for breast and ovarian cancer from different regions of Brazil. Methods: An exploratory, descriptive study was carried out on the prevalence of the BRCA1 and BRCA2 mutations in case series of high risk families to breast and/or ovarian cancer. Following the respective heredogram construction, a sample of blood was taken and DNA extraction was performed to all index cases. The protein truncation test (PTT) was used to screen for truncated mutations in exon 11 of the BRCA1 gene and in exons 10 and 11 of the BRCA2 gene. Results: Of the 612 individuals submitted to genetic testing, 21 (3.4%), 19 women and 2 men, had mutations in the BRCA1 or BRCA2 genes. BRCA1 mutations consisted of ins6kb, 5382insC, 2156delGinsCC, 185delAG, C1201G, C3522T and 3450del4 mutations, while BRCA2 mutations were 5878del10, 5036delA and 4232insA. Conclusion: The prevalence of germline mutations in the BRCA1 and BRCA2 genes found in the present study was lower than reported by other studies in Brazil. The inclusion of individuals with medium risk may have contributed towards the lower observed prevalence. Nevertheless, individuals with a high probability of carrying mutations should be advised with respect to the possibility of undergoing genetic testing, and of the risks, benefits and limitations of these tests. Citation Information: Cancer Res 2009;69(2 Suppl):Abstract nr 6020.

Research paper thumbnail of A contribuição dos genes BRCA na predisposição hereditária ao câncer de mama BRCA Genes Contribution in the hereditary predisposition for breast cancer

Approximately 20% of the cases related to familiar breast cancer are associated to one gene of he... more Approximately 20% of the cases related to familiar breast cancer are associated to one gene of hereditary susceptibility for breast and ovarian cancer, BRCA1 and BRCA2. Recent works have demonstrated the mechanism of action of these genes, with functions well established for maintaining the genome integrity and for controlling homologates recombination. Familiar history, bilaterality, precocious diseases, as well as specific histopathologic results are indicative factors of the presence of mutation in these genes. This literature review summarizes some of this knowledge, trying to understand them in the current functional context of these genes.

Research paper thumbnail of The tale of TILs in breast cancer: A report from The International Immuno-Oncology Biomarker Working Group

npj Breast Cancer

The advent of immune-checkpoint inhibitors (ICI) in modern oncology has significantly improved su... more The advent of immune-checkpoint inhibitors (ICI) in modern oncology has significantly improved survival in several cancer settings. A subgroup of women with breast cancer (BC) has immunogenic infiltration of lymphocytes with expression of programmed death-ligand 1 (PD-L1). These patients may potentially benefit from ICI targeting the programmed death 1 (PD-1)/PD-L1 signaling axis. The use of tumor-infiltrating lymphocytes (TILs) as predictive and prognostic biomarkers has been under intense examination. Emerging data suggest that TILs are associated with response to both cytotoxic treatments and immunotherapy, particularly for patients with triple-negative BC. In this review from The International Immuno-Oncology Biomarker Working Group, we discuss (a) the biological understanding of TILs, (b) their analytical and clinical validity and efforts toward the clinical utility in BC, and (c) the current status of PD-L1 and TIL testing across different continents, including experiences fro...

Research paper thumbnail of RNA Expression Classifiers from a Model of Breast Epithelial Cell Organization to Predict Pathological Complete Response in Triple Negative Breast Cancer

Pathological complete response (pCR) to neoadjuvant chemotherapy (NAC) is correlated with better ... more Pathological complete response (pCR) to neoadjuvant chemotherapy (NAC) is correlated with better outcomes for breast cancer, especially for triple negative breast cancer (TNBC). We developed RNA

Research paper thumbnail of Descarga papilar: relação com o câncer de mama e estratégias para a sua abordagem

Rev Bras Mastologia, Mar 1, 2007

Research paper thumbnail of Clinicopathologic profile of breast cancer patients treated with neoadjuvant chemotherapy at HUCFF/UFRJ

Mastology, 2021

Introduction: The objective of this study is to describe the profile of patients from a public in... more Introduction: The objective of this study is to describe the profile of patients from a public institution, submitted to neoadjuvant chemotherapy (NACT), comparing the verified pathological response with literature data. Methods: Observational retrospective cohort study on breast cancer patients diagnosed between September 2001 and October 2018 and treated with NACT at Hospital Universitário Clementino Fraga Filho (HUCFF/UFRJ), located in Rio de Janeiro, Brazil. The adopted neoadjuvant chemotherapy regimen was based on anthracycline and docetaxel. Results: A total of 133 patients were evaluated. The average age in this group was 54 years (28-86), 49 women (37%) were under 50 years old. The following distribution by molecular subtype was observed: overexpression or amplification of the human epidermal growth factor receptor 2 (HER2+) (13 women, 26.6%), Luminal (19 women, 38.8%), and Triple-negative (TN) (17 women, 34.6%). The HER2+ and TN subtypes had a higher incidence of cases betw...

Research paper thumbnail of Prevalence of BRCA1 and BRCA2 gene mutations in families with medium and high risk of breast and ovarian cancer in Brazil

Brazilian Journal of Medical and Biological Research, 2009

Research paper thumbnail of Prevalence of BRCA1 and BRCA2 genes mutations in a sample of families with high risk of breast and ovarian cancer in Brazil

Cancer Research, 2009

Abstract #6020 Introduction: Of all malignant neoplasia among women, breast cancer has the highes... more Abstract #6020 Introduction: Of all malignant neoplasia among women, breast cancer has the highest incidence rate in Brazil. The objective of the present study was to ascertain the frequency of genetic modifications in a high risk population for breast and ovarian cancer from different regions of Brazil. Methods: An exploratory, descriptive study was carried out on the prevalence of the BRCA1 and BRCA2 mutations in case series of high risk families to breast and/or ovarian cancer. Following the respective heredogram construction, a sample of blood was taken and DNA extraction was performed to all index cases. The protein truncation test (PTT) was used to screen for truncated mutations in exon 11 of the BRCA1 gene and in exons 10 and 11 of the BRCA2 gene. Results: Of the 612 individuals submitted to genetic testing, 21 (3.4%), 19 women and 2 men, had mutations in the BRCA1 or BRCA2 genes. BRCA1 mutations consisted of ins6kb, 5382insC, 2156delGinsCC, 185delAG, C1201G, C3522T and 3450del4 mutations, while BRCA2 mutations were 5878del10, 5036delA and 4232insA. Conclusion: The prevalence of germline mutations in the BRCA1 and BRCA2 genes found in the present study was lower than reported by other studies in Brazil. The inclusion of individuals with medium risk may have contributed towards the lower observed prevalence. Nevertheless, individuals with a high probability of carrying mutations should be advised with respect to the possibility of undergoing genetic testing, and of the risks, benefits and limitations of these tests. Citation Information: Cancer Res 2009;69(2 Suppl):Abstract nr 6020.

Research paper thumbnail of A contribuição dos genes BRCA na predisposição hereditária ao câncer de mama BRCA Genes Contribution in the hereditary predisposition for breast cancer

Approximately 20% of the cases related to familiar breast cancer are associated to one gene of he... more Approximately 20% of the cases related to familiar breast cancer are associated to one gene of hereditary susceptibility for breast and ovarian cancer, BRCA1 and BRCA2. Recent works have demonstrated the mechanism of action of these genes, with functions well established for maintaining the genome integrity and for controlling homologates recombination. Familiar history, bilaterality, precocious diseases, as well as specific histopathologic results are indicative factors of the presence of mutation in these genes. This literature review summarizes some of this knowledge, trying to understand them in the current functional context of these genes.

Research paper thumbnail of The tale of TILs in breast cancer: A report from The International Immuno-Oncology Biomarker Working Group

npj Breast Cancer

The advent of immune-checkpoint inhibitors (ICI) in modern oncology has significantly improved su... more The advent of immune-checkpoint inhibitors (ICI) in modern oncology has significantly improved survival in several cancer settings. A subgroup of women with breast cancer (BC) has immunogenic infiltration of lymphocytes with expression of programmed death-ligand 1 (PD-L1). These patients may potentially benefit from ICI targeting the programmed death 1 (PD-1)/PD-L1 signaling axis. The use of tumor-infiltrating lymphocytes (TILs) as predictive and prognostic biomarkers has been under intense examination. Emerging data suggest that TILs are associated with response to both cytotoxic treatments and immunotherapy, particularly for patients with triple-negative BC. In this review from The International Immuno-Oncology Biomarker Working Group, we discuss (a) the biological understanding of TILs, (b) their analytical and clinical validity and efforts toward the clinical utility in BC, and (c) the current status of PD-L1 and TIL testing across different continents, including experiences fro...

Research paper thumbnail of RNA Expression Classifiers from a Model of Breast Epithelial Cell Organization to Predict Pathological Complete Response in Triple Negative Breast Cancer

Pathological complete response (pCR) to neoadjuvant chemotherapy (NAC) is correlated with better ... more Pathological complete response (pCR) to neoadjuvant chemotherapy (NAC) is correlated with better outcomes for breast cancer, especially for triple negative breast cancer (TNBC). We developed RNA

Research paper thumbnail of Descarga papilar: relação com o câncer de mama e estratégias para a sua abordagem

Rev Bras Mastologia, Mar 1, 2007