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Papers by magharla dasaratha Dhanaraju

Beni-Suef University Journal of Basic and Applied Sciences, 2022

Background Antisense oligonucleotides are a promising novel class of therapeutic agents to treat ... more Background Antisense oligonucleotides are a promising novel class of therapeutic agents to treat different diseases in living things. They provide an efficient method for making target-selective agents because they change gene expression sequences. Therefore, the malfunctioning protein could be stopped, and the source of disease would be obliterated. The existing reviews of antisense oligonucleotides are focusing on discovery, development and concept. However, there is no review paper concerning the latest development of antisense oligonucleotides and their different therapeutic uses. Therefore, the present work has been targeting a comprehensive summary of newly synthesized antisense oligonucleotides and their biological activities. Main body Antisense oligonucleotides are different from traditional therapeutic agents that are planned to interact with mRNA and modulate protein expression through a unique mechanism of action. In the last three decades, several researchers revealed t...

Indo American Journal of Pharmaceutical Research, 2013

Asian Journal of Pharmaceutical and Clinical Research, 2015

Nanostructures of carbon were first observed in 1952, which gained worldwide interest due to thei... more Nanostructures of carbon were first observed in 1952, which gained worldwide interest due to their various physicochemical properties. Carbon nanotubes (CNTs) have found wide applications in the delivery of therapeutic agents such as peptides, proteins, siRNA, nucleic acids, genes, vaccines and also in bone and neural tissue regeneration. Functionalized CNTs have found to be biocompatible. The eye-catching features of these structures are their electronic, mechanical, optical and chemical characteristics, which open a way to future applications and make them good candidates for a wide variety of applications, including drug transporters, new therapeutics, delivery systems and diagnostics. Their unique surface area, stiffness, strength, and resilience have led to much excitement in the field of pharmacy. They can pass through membranes, carrying therapeutic drugs, vaccines, and nucleic acids deep into the cell to targets that are previously unreachable. The applications of carbon nan...

Current Issues in Pharmacy and Medical Sciences, 2018

A simple, fast, and highly selective RP-HPLC method was developed for the determination of Dapagl... more A simple, fast, and highly selective RP-HPLC method was developed for the determination of Dapagliflozin (DAP) and Saxagliptin (SAX) in API and tablet dosage form. The separation was done using a Xterra RP18 (4.6×150 mm, 5 μm particle size) column with Acetonitrile: water (60:40). The isocratic elution mode at a flow rate of 1 mL/min, and the analytes were measured at 248 nm. The retention time for DAP and SAX were about 2.091 and 3.249 min, respectively. Calibration curves were found to be linear in the ranges of 100-500 μg/ml for DAP and 50-250 μg/ml for SAX, with correlation coefficients of 0.9998. The detection and quantification values for DAP was 3.0 and 9.98 μg/ml and SAX was 3.02 and 10 μg/ml respectively.

Journal of Applied Pharmaceutical Science, 2014

This report describes analysis of paclitaxel, which is an antineoplastic drug used in the treatme... more This report describes analysis of paclitaxel, which is an antineoplastic drug used in the treatment of kaposi's sarcoma and cancer of the lung by isocratic high performace liquid chromatography with UV detection in pure form and rat plasma. The analysis was carried out using phenomenex C18 (250 x 4.6 mm, 5 µm particle size) column with a mobile phase consisting of acetonitrile and phosphate buffer (pH 5) in the ratio of 80:20%, v/v. Paclitaxel was eluted at the retention time of 5.3 min when operated at the flow rate of 1 ml/min and monitored by UV at 227 nm. Paclitaxel was extracted from rat plasma by simple LLE method using non-toxic ethyl acetate as extraction solvent. The linearity was accessed in the concentration range of 100-600 µg/mL with correlation coefficient of 0.9999 and percentage recovery of 99.86. The liquid chromatography method was extensively validated for linearity, accuracy, precision, LOD, LOQ and robustness. All these analytical validation parameters were observed to be satisfactory, which indicates the usefulness of method for determination of paclitaxel in pure form and rat plasma. No interfering peaks were observed during the analysis.

Purpose: To formulate biodegradable chitosan microspheres loaded with famotidine to overcome the ... more Purpose: To formulate biodegradable chitosan microspheres loaded with famotidine to overcome the poor bioavailability and frequent dose administration of the drug. Methods: Chitosan microspheres were prepared by simple emulsification technique based on glutaraldehyde crosslinking. Various process and formulation variables such as speed of emulsification, crosslinking time, drug/polymer ratio, volume of cross linking agent and volume of surfactant were optimized. The microspheres were characterized for entrapment efficiency, drug loading, in vitro drug release, surface morphology, as well as by particle size analysis, Fourier transform infrared (FTIR) spectroscopy and differential scanning calorimetry (DSC). Results: The microspheres showed a smooth surface with a narrow particle size distribution (105-219 µm) and an entrapment efficiency of up to 73 %. They exhibited controlled drug release characteristics with 85.6 % of the drug released over a period of 24 h with an initial burst release of 26.9 % in the first 2 h. Drug release followed Higuchi release kinetics. FTIR and DSC data indicate that there was no drug interaction between the drug and polymer used. Conclusion: The chitosan microspheres could be further developed as a potential biodegradable carrier for oral controlled delivery of famotidine.

The objective of this article is to develop a new validated RP-HPLC method for the estimation of ... more The objective of this article is to develop a new validated RP-HPLC method for the estimation of Etoposide, an anti-cancer drug in rat plasma. Etoposide was estimated by a new RP-HPLC method developed on a phenomenex C18 (4.6 x 250 mm, 5µ i.d) column, by isocratic elution using mobile phase mixture of methanol: pH 6 phosphate buffer in a ratio of 80:20 % v/v, at a flow rate 0.9 ml/min and monitored at 273 nm with an average retention time of 4.7 ± 0.1 min. Extraction of the sample from rat plasma was performed by employing liquid-liquid extraction method using chloroform as a solvent. The evaporated chloroform extract was reconstituted by using methanol exhibits linearity in the concentration range of 2000-5000 µg/ml. The LOD and LOQ were found to be 184 µg/ml and 561 µg/ml, respectively with a correlation coefficient of 0.9989 and slope of 1.3818. The method was proved to be more accurate, simple, precise and rapid by statistical validation, extraction and recovery studies.

Beni-Suef University Journal of Basic and Applied Sciences

Background In recent times, a powerful tool of combinatorial synthesis has been used for the prep... more Background In recent times, a powerful tool of combinatorial synthesis has been used for the preparation of large chemical entities through a small set up of reactions between different building blocks using solid-phase and solution-phase techniques. This method reduced the time and cost of the drug discovery process substantially. Main text Thousands of compounds are synthesised in a few reactions through combinatorial synthesis instead of getting a few compounds in the traditional method. This method also helps to identify chemical lead of the compounds and optimise them through the biological screening using a high-throughput method. There is no review concerning the recent research finding of combinatorial synthesis. Hence, an attempt had been made on the latest research findings (2002–2020) of newly synthesised compounds using combinatorial synthesis and their biological activities. Conclusion To the best of our knowledge, the current review has completely analysed the importan...

Future Journal of Pharmaceutical Sciences

Background The main aim of this work was to synthesise a novel N-(substituted phenyl)-2-(3-(hydro... more Background The main aim of this work was to synthesise a novel N-(substituted phenyl)-2-(3-(hydroxyimino) methyl)-1H-indol-1-yl) acetamide derivatives and evaluate their antioxidant activity. These compounds were prepared by a condensation reaction between 1H-indole carbaldehyde oxime and 2-chloro acetamide derivatives. The newly synthesised compound structures were characterised by FT-IR, 1H-NMR, mass spectroscopy and elemental analysis. Furthermore, the above-mentioned compounds were screened for antioxidant activity by using ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) methods. Result The antioxidant activity result reveals that most of the compounds were exhibiting considerable activity in both methods and the values are very closer to the standards. Among the synthesised compounds, compound 3j, 3a and 3k were shown remarkable activity at low concentration. Conclusion Compounds 3j, 3a and 3k were shown highest activity among the prepared anal...

Indian Journal of Pharmacy Practice

Indian Journal of Pharmaceutical Education and Research

Review Journal of Chemistry

Beni-Suef University Journal of Basic and Applied Sciences

Background Neurotransmitters are involved in several functions in the brain and the body of livin... more Background Neurotransmitters are involved in several functions in the brain and the body of living things. Changes in the level of neurotransmitters in the brain are associated with several illnesses. Some of the drugs are controlling the neurotransmitter by adjusting the level in the brain and are exclusively used in the treatment of psychological disorders. The purpose of the study was to find out the effect of novel synthesised phenothiazine derivatives (GC1, GC2 and GC8) either alone (7.5 mg/kg or 15 mg/kg, oral) or in combination with amphetamine on the experimental animals. Results Dopamine level in rat brain was estimated by a spectroscopic method using the UV-visible double beam spectrophotometer at 735 nm. The results revealed that these derivatives blocked the brain dopamine level significantly. The compound GC8 (15 mg/kg) significantly reduced the level of dopamine (0.151 ± 0.04, 0.284 ± 0.03) as similar to that of a standard drug. Furthermore, compounds GC2 (15 mg/kg) an...

Egyptian Journal of Basic and Applied Sciences

The purpose of the study is to design, synthesise and assess the antipsychotic activity of a set ... more The purpose of the study is to design, synthesise and assess the antipsychotic activity of a set of the novel (5-(10-(3-N, N-Dimethylamino) propyl)-10H-phenothiazine-3-yl)-1,3,4-thiadiazo-2-yl) Azodye/Schiff base/Chalcone derivatives. The newly synthesised compound structure was characterised by FT-IR, 1 H NMR, Mass spectroscopy and elemental analysis. Each compound has been shown an excellent antipsychotic activity in a haloperidol-induced catalepsy metallic bar test. The results found are firmly similar to docking study. Among the synthesised derivatives, compound 2-Amino-6-(3-hydroxy-4-methyl phenyl) pyrimidine-4-yl) (7-chloro-10-(3-(N, N-dimethylamino) propyl)-10H-phenothiazine-3-yl) methanone (GC8) exhibiting high potency of catalepsy induction. Therefore, the derivative of GC8 has been considered that a potent anti-psychotic agent among the synthesised compounds.

Asian Pacific Journal of Cancer Prevention

Objective: To investigate the biogenic synthesis of silver nanoparticles (AgNPs) using partially ... more Objective: To investigate the biogenic synthesis of silver nanoparticles (AgNPs) using partially purified ethyl acetate extract of Nannochloropsis sp. hexane (EAENH) fraction of microalga. Methods: The green synthesis of AgNPs was confirmed with UV-Vis spectrum which shows the surface plasmon resonance (SPR) at 421 nm. Fourier Transform Infrared Spectra (FTIR) presented the involvement of functional groups like carboxyl groups of fatty acids, tetraterpenoids of xanthophylls, hydroxyl groups of polyphenols, carbonyl and amide linkage of proteins in the AgNP synthesis. Gas Chromatography-Mass Spectrometry analysis (GCMS) revealed that phytochemicals like octadecanoic acid and hexadecanoic acid imply in capping, bioreduction, and stabilization of AgNps. Result: High-resolution Transmission electron microscope (HRTEM), Dynamic light scattering (DLS), X-ray diffraction (XRD) and EDX analysis showed the crystalline form of the AgNPs with Z-average size 57.25 nm. The zeta potential value of-25.7 mV demonstrated the negative surface charge and colloidal stability of AgNPs. The antimicrobial activity of AgNPs displayed effective inhibition zone against selected bacterial and fungal pathogens. In vitro, antioxidant effects were assessed by 1,1-diphenyl-2-picryl-hydrazyl (DPPH), hydrogen peroxide and reducing power assays which revealed excellent scavenging potential for AgNPs than the extracts. The anti-proliferative potential of biofabricated AgNPs and extracts on Human Non-small lung cancer cell line (A549) was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyl-tetrazolium bromide (MTT) assay with IC 50 values of 15 µgmL-1 and 175 µgmL-1 respectively. Conclusion: The study reveals that the microalgae-mediated AgNPs possesses potent antimicrobial and antioxidant activity along with the ability to stimulate apoptosis in A-549 cell line.

Indian Journal of Science and Technology

Review Journal of Chemistry

Beni-Suef University Journal of Basic and Applied Sciences

Current Issues in Pharmacy and Medical Sciences

A simple, fast, and highly selective RP-HPLC method was developed for the determination of Dapagl... more A simple, fast, and highly selective RP-HPLC method was developed for the determination of Dapagliflozin (DAP) and Saxagliptin (SAX) in API and tablet dosage form. The separation was done using a Xterra RP18 (4.6×150 mm, 5 μm particle size) column with Acetonitrile: water (60:40). The isocratic elution mode at a flow rate of 1 mL/min, and the analytes were measured at 248 nm. The retention time for DAP and SAX were about 2.091 and 3.249 min, respectively. Calibration curves were found to be linear in the ranges of 100-500 μg/ml for DAP and 50-250 μg/ml for SAX, with correlation coefficients of 0.9998. The detection and quantification values for DAP was 3.0 and 9.98 μg/ml and SAX was 3.02 and 10 μg/ml respectively.

Future Journal of Pharmaceutical Sciences

Beni-Suef University Journal of Basic and Applied Sciences, 2022

Background Antisense oligonucleotides are a promising novel class of therapeutic agents to treat ... more Background Antisense oligonucleotides are a promising novel class of therapeutic agents to treat different diseases in living things. They provide an efficient method for making target-selective agents because they change gene expression sequences. Therefore, the malfunctioning protein could be stopped, and the source of disease would be obliterated. The existing reviews of antisense oligonucleotides are focusing on discovery, development and concept. However, there is no review paper concerning the latest development of antisense oligonucleotides and their different therapeutic uses. Therefore, the present work has been targeting a comprehensive summary of newly synthesized antisense oligonucleotides and their biological activities. Main body Antisense oligonucleotides are different from traditional therapeutic agents that are planned to interact with mRNA and modulate protein expression through a unique mechanism of action. In the last three decades, several researchers revealed t...

Indo American Journal of Pharmaceutical Research, 2013

Asian Journal of Pharmaceutical and Clinical Research, 2015

Nanostructures of carbon were first observed in 1952, which gained worldwide interest due to thei... more Nanostructures of carbon were first observed in 1952, which gained worldwide interest due to their various physicochemical properties. Carbon nanotubes (CNTs) have found wide applications in the delivery of therapeutic agents such as peptides, proteins, siRNA, nucleic acids, genes, vaccines and also in bone and neural tissue regeneration. Functionalized CNTs have found to be biocompatible. The eye-catching features of these structures are their electronic, mechanical, optical and chemical characteristics, which open a way to future applications and make them good candidates for a wide variety of applications, including drug transporters, new therapeutics, delivery systems and diagnostics. Their unique surface area, stiffness, strength, and resilience have led to much excitement in the field of pharmacy. They can pass through membranes, carrying therapeutic drugs, vaccines, and nucleic acids deep into the cell to targets that are previously unreachable. The applications of carbon nan...

Current Issues in Pharmacy and Medical Sciences, 2018

A simple, fast, and highly selective RP-HPLC method was developed for the determination of Dapagl... more A simple, fast, and highly selective RP-HPLC method was developed for the determination of Dapagliflozin (DAP) and Saxagliptin (SAX) in API and tablet dosage form. The separation was done using a Xterra RP18 (4.6×150 mm, 5 μm particle size) column with Acetonitrile: water (60:40). The isocratic elution mode at a flow rate of 1 mL/min, and the analytes were measured at 248 nm. The retention time for DAP and SAX were about 2.091 and 3.249 min, respectively. Calibration curves were found to be linear in the ranges of 100-500 μg/ml for DAP and 50-250 μg/ml for SAX, with correlation coefficients of 0.9998. The detection and quantification values for DAP was 3.0 and 9.98 μg/ml and SAX was 3.02 and 10 μg/ml respectively.

Journal of Applied Pharmaceutical Science, 2014

This report describes analysis of paclitaxel, which is an antineoplastic drug used in the treatme... more This report describes analysis of paclitaxel, which is an antineoplastic drug used in the treatment of kaposi's sarcoma and cancer of the lung by isocratic high performace liquid chromatography with UV detection in pure form and rat plasma. The analysis was carried out using phenomenex C18 (250 x 4.6 mm, 5 µm particle size) column with a mobile phase consisting of acetonitrile and phosphate buffer (pH 5) in the ratio of 80:20%, v/v. Paclitaxel was eluted at the retention time of 5.3 min when operated at the flow rate of 1 ml/min and monitored by UV at 227 nm. Paclitaxel was extracted from rat plasma by simple LLE method using non-toxic ethyl acetate as extraction solvent. The linearity was accessed in the concentration range of 100-600 µg/mL with correlation coefficient of 0.9999 and percentage recovery of 99.86. The liquid chromatography method was extensively validated for linearity, accuracy, precision, LOD, LOQ and robustness. All these analytical validation parameters were observed to be satisfactory, which indicates the usefulness of method for determination of paclitaxel in pure form and rat plasma. No interfering peaks were observed during the analysis.

Purpose: To formulate biodegradable chitosan microspheres loaded with famotidine to overcome the ... more Purpose: To formulate biodegradable chitosan microspheres loaded with famotidine to overcome the poor bioavailability and frequent dose administration of the drug. Methods: Chitosan microspheres were prepared by simple emulsification technique based on glutaraldehyde crosslinking. Various process and formulation variables such as speed of emulsification, crosslinking time, drug/polymer ratio, volume of cross linking agent and volume of surfactant were optimized. The microspheres were characterized for entrapment efficiency, drug loading, in vitro drug release, surface morphology, as well as by particle size analysis, Fourier transform infrared (FTIR) spectroscopy and differential scanning calorimetry (DSC). Results: The microspheres showed a smooth surface with a narrow particle size distribution (105-219 µm) and an entrapment efficiency of up to 73 %. They exhibited controlled drug release characteristics with 85.6 % of the drug released over a period of 24 h with an initial burst release of 26.9 % in the first 2 h. Drug release followed Higuchi release kinetics. FTIR and DSC data indicate that there was no drug interaction between the drug and polymer used. Conclusion: The chitosan microspheres could be further developed as a potential biodegradable carrier for oral controlled delivery of famotidine.

The objective of this article is to develop a new validated RP-HPLC method for the estimation of ... more The objective of this article is to develop a new validated RP-HPLC method for the estimation of Etoposide, an anti-cancer drug in rat plasma. Etoposide was estimated by a new RP-HPLC method developed on a phenomenex C18 (4.6 x 250 mm, 5µ i.d) column, by isocratic elution using mobile phase mixture of methanol: pH 6 phosphate buffer in a ratio of 80:20 % v/v, at a flow rate 0.9 ml/min and monitored at 273 nm with an average retention time of 4.7 ± 0.1 min. Extraction of the sample from rat plasma was performed by employing liquid-liquid extraction method using chloroform as a solvent. The evaporated chloroform extract was reconstituted by using methanol exhibits linearity in the concentration range of 2000-5000 µg/ml. The LOD and LOQ were found to be 184 µg/ml and 561 µg/ml, respectively with a correlation coefficient of 0.9989 and slope of 1.3818. The method was proved to be more accurate, simple, precise and rapid by statistical validation, extraction and recovery studies.

Beni-Suef University Journal of Basic and Applied Sciences

Background In recent times, a powerful tool of combinatorial synthesis has been used for the prep... more Background In recent times, a powerful tool of combinatorial synthesis has been used for the preparation of large chemical entities through a small set up of reactions between different building blocks using solid-phase and solution-phase techniques. This method reduced the time and cost of the drug discovery process substantially. Main text Thousands of compounds are synthesised in a few reactions through combinatorial synthesis instead of getting a few compounds in the traditional method. This method also helps to identify chemical lead of the compounds and optimise them through the biological screening using a high-throughput method. There is no review concerning the recent research finding of combinatorial synthesis. Hence, an attempt had been made on the latest research findings (2002–2020) of newly synthesised compounds using combinatorial synthesis and their biological activities. Conclusion To the best of our knowledge, the current review has completely analysed the importan...

Future Journal of Pharmaceutical Sciences

Background The main aim of this work was to synthesise a novel N-(substituted phenyl)-2-(3-(hydro... more Background The main aim of this work was to synthesise a novel N-(substituted phenyl)-2-(3-(hydroxyimino) methyl)-1H-indol-1-yl) acetamide derivatives and evaluate their antioxidant activity. These compounds were prepared by a condensation reaction between 1H-indole carbaldehyde oxime and 2-chloro acetamide derivatives. The newly synthesised compound structures were characterised by FT-IR, 1H-NMR, mass spectroscopy and elemental analysis. Furthermore, the above-mentioned compounds were screened for antioxidant activity by using ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) methods. Result The antioxidant activity result reveals that most of the compounds were exhibiting considerable activity in both methods and the values are very closer to the standards. Among the synthesised compounds, compound 3j, 3a and 3k were shown remarkable activity at low concentration. Conclusion Compounds 3j, 3a and 3k were shown highest activity among the prepared anal...

Indian Journal of Pharmacy Practice

Indian Journal of Pharmaceutical Education and Research

Review Journal of Chemistry

Beni-Suef University Journal of Basic and Applied Sciences

Background Neurotransmitters are involved in several functions in the brain and the body of livin... more Background Neurotransmitters are involved in several functions in the brain and the body of living things. Changes in the level of neurotransmitters in the brain are associated with several illnesses. Some of the drugs are controlling the neurotransmitter by adjusting the level in the brain and are exclusively used in the treatment of psychological disorders. The purpose of the study was to find out the effect of novel synthesised phenothiazine derivatives (GC1, GC2 and GC8) either alone (7.5 mg/kg or 15 mg/kg, oral) or in combination with amphetamine on the experimental animals. Results Dopamine level in rat brain was estimated by a spectroscopic method using the UV-visible double beam spectrophotometer at 735 nm. The results revealed that these derivatives blocked the brain dopamine level significantly. The compound GC8 (15 mg/kg) significantly reduced the level of dopamine (0.151 ± 0.04, 0.284 ± 0.03) as similar to that of a standard drug. Furthermore, compounds GC2 (15 mg/kg) an...

Egyptian Journal of Basic and Applied Sciences

The purpose of the study is to design, synthesise and assess the antipsychotic activity of a set ... more The purpose of the study is to design, synthesise and assess the antipsychotic activity of a set of the novel (5-(10-(3-N, N-Dimethylamino) propyl)-10H-phenothiazine-3-yl)-1,3,4-thiadiazo-2-yl) Azodye/Schiff base/Chalcone derivatives. The newly synthesised compound structure was characterised by FT-IR, 1 H NMR, Mass spectroscopy and elemental analysis. Each compound has been shown an excellent antipsychotic activity in a haloperidol-induced catalepsy metallic bar test. The results found are firmly similar to docking study. Among the synthesised derivatives, compound 2-Amino-6-(3-hydroxy-4-methyl phenyl) pyrimidine-4-yl) (7-chloro-10-(3-(N, N-dimethylamino) propyl)-10H-phenothiazine-3-yl) methanone (GC8) exhibiting high potency of catalepsy induction. Therefore, the derivative of GC8 has been considered that a potent anti-psychotic agent among the synthesised compounds.

Asian Pacific Journal of Cancer Prevention

Objective: To investigate the biogenic synthesis of silver nanoparticles (AgNPs) using partially ... more Objective: To investigate the biogenic synthesis of silver nanoparticles (AgNPs) using partially purified ethyl acetate extract of Nannochloropsis sp. hexane (EAENH) fraction of microalga. Methods: The green synthesis of AgNPs was confirmed with UV-Vis spectrum which shows the surface plasmon resonance (SPR) at 421 nm. Fourier Transform Infrared Spectra (FTIR) presented the involvement of functional groups like carboxyl groups of fatty acids, tetraterpenoids of xanthophylls, hydroxyl groups of polyphenols, carbonyl and amide linkage of proteins in the AgNP synthesis. Gas Chromatography-Mass Spectrometry analysis (GCMS) revealed that phytochemicals like octadecanoic acid and hexadecanoic acid imply in capping, bioreduction, and stabilization of AgNps. Result: High-resolution Transmission electron microscope (HRTEM), Dynamic light scattering (DLS), X-ray diffraction (XRD) and EDX analysis showed the crystalline form of the AgNPs with Z-average size 57.25 nm. The zeta potential value of-25.7 mV demonstrated the negative surface charge and colloidal stability of AgNPs. The antimicrobial activity of AgNPs displayed effective inhibition zone against selected bacterial and fungal pathogens. In vitro, antioxidant effects were assessed by 1,1-diphenyl-2-picryl-hydrazyl (DPPH), hydrogen peroxide and reducing power assays which revealed excellent scavenging potential for AgNPs than the extracts. The anti-proliferative potential of biofabricated AgNPs and extracts on Human Non-small lung cancer cell line (A549) was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyl-tetrazolium bromide (MTT) assay with IC 50 values of 15 µgmL-1 and 175 µgmL-1 respectively. Conclusion: The study reveals that the microalgae-mediated AgNPs possesses potent antimicrobial and antioxidant activity along with the ability to stimulate apoptosis in A-549 cell line.

Indian Journal of Science and Technology

Review Journal of Chemistry

Beni-Suef University Journal of Basic and Applied Sciences

Current Issues in Pharmacy and Medical Sciences

A simple, fast, and highly selective RP-HPLC method was developed for the determination of Dapagl... more A simple, fast, and highly selective RP-HPLC method was developed for the determination of Dapagliflozin (DAP) and Saxagliptin (SAX) in API and tablet dosage form. The separation was done using a Xterra RP18 (4.6×150 mm, 5 μm particle size) column with Acetonitrile: water (60:40). The isocratic elution mode at a flow rate of 1 mL/min, and the analytes were measured at 248 nm. The retention time for DAP and SAX were about 2.091 and 3.249 min, respectively. Calibration curves were found to be linear in the ranges of 100-500 μg/ml for DAP and 50-250 μg/ml for SAX, with correlation coefficients of 0.9998. The detection and quantification values for DAP was 3.0 and 9.98 μg/ml and SAX was 3.02 and 10 μg/ml respectively.

Future Journal of Pharmaceutical Sciences