mahdi esmaeilizadeh - Academia.edu (original) (raw)
Papers by mahdi esmaeilizadeh
DOAJ (DOAJ: Directory of Open Access Journals), Jul 28, 2016
Objective: In this study, we aimed at evaluation of the efficacy of Hypericum perforatum and flax... more Objective: In this study, we aimed at evaluation of the efficacy of Hypericum perforatum and flaxseed on hot flash, vaginal atrophy and estrogen-dependent cancers in menopausal women Materials and Methods: We searched MEDLINE, Scopus, and the Cochrane Central Register of Controlled Trials (RCT) to explore trials that assessed the effectiveness of H. perforatum and flaxseed on hot flash, vaginal atrophy and estrogen-dependent cancers. In this regard, the following terms were used "menopause AND H. perforatum OR flaxseed OR Linum usitatissimum. Only randomized controlled trials were included in the study. Results: Nine RCTs were included in this systematic review. Based on the literature, flaxseed showed beneficial effect on hot flash frequency and intensity, which was not statistically significant. According to two trials, flaxseed showed estrogenic effects; however, no conclusion regarding cancer promoting or protecting effects can be made. The evidence of the efficacy of the flaxseed on alleviating vaginal atrophy was also limited due to inconsistent findings in this regard. One trial declared that Vitex agnus-castus and H. perforatum showed comparable decrease in the frequency of hot flashes. Conclusion: The results of our systematic review suggest beneficial effect on vasomotor symptom with both of flaxseed and H. perforatum. Consistent conclusion regarding estrogendependent cancers and maturation value is limited due to small number of trials related to flaxseed. Further trials are still needed to confirm the results of our systematic review.
Journal of Bodywork and Movement Therapies, Jul 1, 2016
Exercise-induced asthma is seen following vigorous or prolonged exercise or phys ical exertion. I... more Exercise-induced asthma is seen following vigorous or prolonged exercise or phys ical exertion. It has been suggested that climatic conditions have an influence on exerciseinduced asthma. Therefore, the aim of the present study was to examine the effect of two cli matic conditions on exercise-induced deterioration of pulmonary function tests in 10e12 year old students. Two hundred and fifty six students were randomly chosen from two cities namely Kerman and Gorgan (128 subjects in each who were equally from both cities) including 62 girls and 66 boys of 10e12 years old. A questionnaire was used to obtain demographic information and to identify the prevalence of asthma symptoms. Each subject performed a sevenminute free run exercise with maximum effort and sufficient motivation until they reached 70e75% heart rate. Pulmonary function tests (PFT) including, forced vital capacity (FVC), forced expiratory volume in one second (FEV 1), peak expiratory flow (PEF), and maximum expi ratory flow at 50% of vital capacity (MEF 50
shiraz e medical journal, Nov 29, 2017
Background: Cyclophosphamide (CP) is one of the most popular bifunctional alkylating agents, whic... more Background: Cyclophosphamide (CP) is one of the most popular bifunctional alkylating agents, which is utilized in the treatment of numerous cancer diseases and has toxic side effects such as nephrotoxicity, hematotoxicity, mutagenicity, and immunotoxicity. In this study, Elaeagnus angustifolia fruit extract (EAFE), a natural plant extract, was examined for its antioxidant activity against CP-induced renal oxidative damage in mice. Methods: In this experimental study, 30 male Swiss albino mice (25-30 g) were separated into five groups. Group 1 received normal saline for 5 days; group 2 was treated with CP 200 mg/kg single intraperitoneal (i.p) dose only on the 5th day; groups 3 to 5 received EAFE at doses of 100, 200, and 400 mg/kg, respectively, administered orally during 5 days, and CP on the 5th day 1 hour after the last dose of extract administration. Then on the 6th day, the mice were sacrificed. Blood samples were collected to determine serum blood urea nitrogen (BUN) and creatinine (Cr) levels. Levels of malondialdehyde (MDA) and glutathione (GSH) in kidney tissue were also examined. Histological assessment was undertaken on kidney samples taken from all groups. Results: The results obtained from our study showed a significant increase in the levels of MDA, Cr, and BUN and a decrease in GSH following CP injection. Pre-treatment with EAFE showed a decrease in the levels of MDA, Cr, and BUN and an increase in GSH in all doses; however, the most significant reduction was observed at doses of 200 and 400 mg/kg. In addition, the nephroprotective effect of EAFE was confirmed by histological evaluation of the kidneys. Conclusions: Our results indicate that EAFE has protective effects against CP-induced renal damage possibly by amelioration of biochemical indices and oxidative stress parameters.
DOAJ (DOAJ: Directory of Open Access Journals), Oct 16, 2015
Objective: Oxidative stress damages cells and brings about the pathogenesis of ischemia/reperfusi... more Objective: Oxidative stress damages cells and brings about the pathogenesis of ischemia/reperfusion injury. This study was carried out to investigate the preconditioning and cardio protective potential effects of crocin and vitamin E by the eNOS and iNOS express gene in ischemia/reperfusion in rats. Material & Methods: Male rats were divided into seven groups, namely: sham, control group and experimental groups treated with crocin(10, 20 and 40 mg/kg), vitamin E (100 mg/kg) and combination of crocin (40 mg/kg) with vitamin E (100 mg/kg) that were gavaged The heart was removed and relocated to a Langendorff apparatus and subjected to global ischemia and then the left ventricular end diastolic pressure (LVEDP) were measured as a hemodynamic parameter. Total RNA was extracted from heart frozen tissues. RT-PCR technique was performed by specific primers designed for nitric oxide gene and the results were assessed by agarose gel electrophoresis. Results: Results after ischemia and reperfusion showed that crocin 40 mg/kg produced a significant improvement of LVEDP as a mechanical function (p<0.05), associated with a reduction of iNOS release (p<0.05). The eNOS mRNA levels were significantly higher in crocin-treated 40 mg/kg compared to controls treated by RT-PCR technique. The combination of crocin and vitamin E have shown more effective on the reduction of iNOS release (p<0.01). Conclusion: In the isolated rat heart, protective effect of crocin, may possibly be explained by regulating eNOS and iNOS expressions. The Results resultsconfirmed the hypothesis that cardioprotective effect of crocin is partly mediated by nitric oxide. This could explain the cardioprotective action of crocin following ischemia and reperfusion.
Research in Pharmaceutical Sciences, 2019
Previous studies have shown that some plants in the genus of Ferula (Apiaceae) have antidiabetic ... more Previous studies have shown that some plants in the genus of Ferula (Apiaceae) have antidiabetic effects. The present work was aimed to evaluate effects of Ferula gummosa oleo-resin in a rat model of streptozotocin-induced diabetes. Male Wistar rats were randomized into five groups (n = 6): normal control, diabetic control, diabetic rats treated with insulin (3 IU/day), and diabetic rats treated with 100 or 400 mg/kg/day of an ethanolic extract of the oleo-resin. After 4 weeks, blood samples were collected for measuring fasting blood glucose (FBG), lipid profile, aspartate aminotransferase, alanine aminotransferase (ALT), alkaline phosphatase, blood urea nitrogen, and creatinine. In addition, levels of lipid peroxidation, thiol groups, and superoxide dismutase (SOD) activity were evaluated in the liver and kidney. At the end of the fourth week, the level of FBG in rats treated with 100 mg/kg of the extract was lower than that in diabetic control rats (273 ± 39 mg/dL vs 471 ± 32 mg/dL). Administration of insulin and the extract had no significant effects on the serum lipids. Insulin and both doses of the extract significantly reduced the activity of ALT. In addition, the extract inhibited lipid peroxidation in the kidney and restored the elevated level of SOD in the liver and kidneys. Ferula gummosa oleo-resin has the potential to prevent or delay the complications of diabetes by inhibiting the progression of hyperglycemia and attenuating oxidative stressinduced damage in the liver and kidneys.
Jundishapur Scientific Medical Journal, Nov 21, 2012
Biomedicine & Pharmacotherapy
Elsevier Masson, Dec 31, 2017
Effect of crocin on nitric oxide synthase expression in post-ischemic isolated rat heart
Avicenna Journal of Phytomedicine, 2016
Objective: In this study, we aimed at evaluation of the efficacy of Hypericum perforatum and flax... more Objective: In this study, we aimed at evaluation of the efficacy of Hypericum perforatum and flaxseed on hot flash, vaginal atrophy and estrogen-dependent cancers in menopausal women Materials and Methods: We searched MEDLINE, Scopus, and the Cochrane Central Register of Controlled Trials (RCT) to explore trials that assessed the effectiveness of H. perforatum and flaxseed on hot flash, vaginal atrophy and estrogen-dependent cancers. In this regard, the following terms were used “menopause AND H. perforatum OR flaxseed OR Linum usitatissimum. Only randomized controlled trials were included in the study. Results: Nine RCTs were included in this systematic review. Based on the literature, flaxseed showed beneficial effect on hot flash frequency and intensity, which was not statistically significant. According to two trials, flaxseed showed estrogenic effects; however, no conclusion regarding cancer promoting or protecting effects can be made. The evidence of the efficacy of the flaxse...
[
a Department of Exercise Physiology, Faculty of Physical Education & Sport Sciences, Shahid Bahon... more a Department of Exercise Physiology, Faculty of Physical Education & Sport Sciences, Shahid Bahonar University of Kerman, Kerman, Iran b Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran c Neonatal and Children’s Health Research Center, Golestan University of Medical Sciences, Gorgan, Iran d Student Research Committee, Esfarayen Faculty of Medical Sciences, Esfarayen, Iran e Neurogenic Inflammation Research Centre and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Background and Objective: Bleeding from esophageal varicose (EV) is one of the lethal complicatio... more Background and Objective: Bleeding from esophageal varicose (EV) is one of the lethal complications of liver cirrhosis. Upper gastro-intestinal endoscopy (UGIE) is performed in all cirrhotic patients for screening EV but recently some non-invasive methods have been evaluated as a substitute. For this purpose the aim of this study was to assess the correlation between EV and reno-vascular resistance index (RRI) in cirrhotic patients. Subjects and Methods: Sixty four cirrhotic patients with normal renal function were included and underwent UGIE and measuring RRI using color flow and pulsed wave Doppler ultrasonography. Correlation between the mean of RRI was evaluated with presence and grade of EV. P<0.05 was considered statistically significant. Results: EV was detected in 42 patients (65.5%). The means of RRI were 0.68±0.03 versus 0.62±0.04 in patients with or without EV, respectively (P<0.001). Moreover, there was a significant statistically difference between the mean of ...
Background and Objective: Ulcerative colitis is one of the inflammatory bowel diseases with unkno... more Background and Objective: Ulcerative colitis is one of the inflammatory bowel diseases with unknown etiology. Studies done in different parts of the world showed that the sites of colonic involvement and epidemiology of this disease are varied. The aim of this study was to investigate the pattern of colonic involvement and epidemiologic charateristics of patient referred to Imam Khomeini hospital of Ahvaz during the years 2007-2011. Subjects and Methods: In this retrospective study, the data from 98 patients, who referred to Imam Khomeini hospital during these 5 years with ulcerative colitis, which was confirmed by colonoscopy or pathology, were investigated and analyzed. Results: Mean age of patients was 38.95±2.14 years, 42.8 % were in age group of 31-50 years and male to female ratio was 1.18. The most frequent site of colonic involvement was rectosigmoid (21.4 %) and pancolit (19.4 %). Although 52 % of patients had no co-existing disease, anemia was the most prevalent co-existin...
Avicenna Journal of Phytomedicine, 2015
Objective: Oxidative stress damages cells and brings about the pathogenesis of ischemia/reperfusi... more Objective: Oxidative stress damages cells and brings about the pathogenesis of ischemia/reperfusion injury. This study was carried out to investigate the preconditioning and cardio protective potential effects of crocin and vitamin E by the eNOS and iNOS express gene in ischemia/reperfusion in rats. Material & Methods: Male rats were divided into seven groups, namely: sham, control group and experimental groups treated with crocin(10, 20 and 40 mg/kg), vitamin E (100 mg/kg) and combination of crocin (40 mg/kg) with vitamin E (100 mg/kg) that were gavaged The heart was removed and relocated to a Langendorff apparatus and subjected to global ischemia and then the left ventricular end diastolic pressure (LVEDP) were measured as a hemodynamic parameter. Total RNA was extracted from heart frozen tissues. RT-PCR technique was performed by specific primers designed for nitric oxide gene and the results were assessed by agarose gel electrophoresis. Results: Results after ischemia and reperf...
Journal of Mazandaran University of Medical Sciences, 2019
Background and purpose: Occlusion is a decreasing factor on longevity of peripheral intravenous c... more Background and purpose: Occlusion is a decreasing factor on longevity of peripheral intravenous catheters (PIVCs). Continuous flushing (injection of specific volume of solution such as Normal Saline (N/S) before and after each drug infusion into PIVCs at determined interval time) is one of the effective methods in in vitro studies to maintain the PIVCs patency. The aim of this study was to investigate the effect of continuous flushing on the duration of PIVCs patency. Materials and methods: In a randomized controlled trail, 72 patients hospitalized in medical/surgical department in Esfarayen Imam Khomeini Hospital, north east of Iran, were randomly assigned into either an intervention group or a control group. In intervention group, 5 ml of N/S solution was injected within 5 seconds before and after injection of any drug into the PIVCs. If another drug was administered at the same time, it was injected and flushing technique was performed again. The PIVCs patency was monitored every...
Shiraz E-Medical Journal, 2017
Background: Cyclophosphamide (CP) is one of the most popular bifunctional alkylating agents, whic... more Background: Cyclophosphamide (CP) is one of the most popular bifunctional alkylating agents, which is utilized in the treatment of numerous cancer diseases and has toxic side effects such as nephrotoxicity, hematotoxicity, mutagenicity, and immunotoxicity. In this study, Elaeagnus angustifolia fruit extract (EAFE), a natural plant extract, was examined for its antioxidant activity against CP-induced renal oxidative damage in mice. Methods: In this experimental study, 30 male Swiss albino mice (25-30 g) were separated into five groups. Group 1 received normal saline for 5 days; group 2 was treated with CP 200 mg/kg single intraperitoneal (i.p) dose only on the 5th day; groups 3 to 5 received EAFE at doses of 100, 200, and 400 mg/kg, respectively, administered orally during 5 days, and CP on the 5th day 1 hour after the last dose of extract administration. Then on the 6th day, the mice were sacrificed. Blood samples were collected to determine serum blood urea nitrogen (BUN) and creatinine (Cr) levels. Levels of malondialdehyde (MDA) and glutathione (GSH) in kidney tissue were also examined. Histological assessment was undertaken on kidney samples taken from all groups. Results: The results obtained from our study showed a significant increase in the levels of MDA, Cr, and BUN and a decrease in GSH following CP injection. Pre-treatment with EAFE showed a decrease in the levels of MDA, Cr, and BUN and an increase in GSH in all doses; however, the most significant reduction was observed at doses of 200 and 400 mg/kg. In addition, the nephroprotective effect of EAFE was confirmed by histological evaluation of the kidneys. Conclusions: Our results indicate that EAFE has protective effects against CP-induced renal damage possibly by amelioration of biochemical indices and oxidative stress parameters.
Journal of Nephropathology, 2017
Background: Cyclophosphamide (CP) is amongst the most effective alkylating anticancer drugs which... more Background: Cyclophosphamide (CP) is amongst the most effective alkylating anticancer drugs which regardless of its harmful side effects including nephrotoxicity, hematotoxicity, mutagenicity and also immunotoxicity, commonly raised for the treatment of a number of cancers and autoimmune ailments. Objectives: This study was undertaken to investigate the ameliorative effect of Lavandula officinalis L extract (LOE) in nephrotoxicity induced by CP in mice. Materials and Methods: In this experimental study, 35 male Swiss albino mice (20-25 g) were randomly divided into 5 groups, each group consist of 7 mice. Mice were pretreated with LOE orally in doses of 100, 200 and 400 mg/kg for 5 consecutive days and CP (200 mg/kg, ip) was administrated on the fifth day 1 hour after the last dose of extract. Then on the sixth day, animals were sacrificed. Blood samples were collected to determine serum creatinine (Cr) and blood urea nitrogen (BUN) levels. Malondialdehyde (MDA), glutathione (GSH) levels and catalase (CAT) and superoxide dismutase (SOD) activity were assayed in kidney tissue. The kidney was maintained in formalin for histological examination. Results: Results showed a significant increase in the levels of MDA, Cr and BUN, and decrease of GSH, CAT and SOD by CP administration. Pre-treatment with LOE showed reduction in the levels of MDA, Cr and BUN and increase of GSH, CAT and SOD in all doses but the most significant alteration was observed at doses of 200 and 400 mg/ kg (P < 0.05). Additionally the nephroprotective effect of the LOE was established by the histological examination of the kidneys. Conclusions: Our results indicated that LOE has produced amelioration in biochemical indices and oxidative stress parameters against CP-induced nephrotoxicity.
Research in Pharmaceutical Sciences, 2019
Previous studies have shown that some plants in the genus of Ferula (Apiaceae) have antidiabetic ... more Previous studies have shown that some plants in the genus of Ferula (Apiaceae) have antidiabetic effects. The present work was aimed to evaluate effects of Ferula gummosa oleo-resin in a rat model of streptozotocin-induced diabetes. Male Wistar rats were randomized into five groups (n = 6): normal control, diabetic control, diabetic rats treated with insulin (3 IU/day), and diabetic rats treated with 100 or 400 mg/kg/day of an ethanolic extract of the oleo-resin. After 4 weeks, blood samples were collected for measuring fasting blood glucose (FBG), lipid profile, aspartate aminotransferase, alanine aminotransferase (ALT), alkaline phosphatase, blood urea nitrogen, and creatinine. In addition, levels of lipid peroxidation, thiol groups, and superoxide dismutase (SOD) activity were evaluated in the liver and kidney. At the end of the fourth week, the level of FBG in rats treated with 100 mg/kg of the extract was lower than that in diabetic control rats (273 ± 39 mg/dL vs 471 ± 32 mg/dL). Administration of insulin and the extract had no significant effects on the serum lipids. Insulin and both doses of the extract significantly reduced the activity of ALT. In addition, the extract inhibited lipid peroxidation in the kidney and restored the elevated level of SOD in the liver and kidneys. Ferula gummosa oleo-resin has the potential to prevent or delay the complications of diabetes by inhibiting the progression of hyperglycemia and attenuating oxidative stressinduced damage in the liver and kidneys.
International Journal for Vitamin and Nutrition Research, 2019
. Background: The effects of Vit C on liver and renal function and the tissues oxidative damage w... more . Background: The effects of Vit C on liver and renal function and the tissues oxidative damage was investigated in hypothyroid rats. Materials and methods: The pregnant rats were divided into 5 groups (n=6): (1) Control; (2) Propylthiouracil (PTU; 0.005%), (3–5) PTU plus 10, 100 or 500 mg/kg b.w. Vit C. The drugs were added to the drinking water of the dams and their pups during lactation period and then continued for the offspring through the first 8 weeks of their life. Finally, 7 male offspring from each group were randomly selected. Results: Thyroxine, protein and albumin concentrations in the serum and thiol content and superoxide dismutase (SOD) and catalase (CAT) activities in renal and liver tissues of hypothyroid group was lower (all P<0.001) while, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALK-P), creatinine and blood urea nitrogen (BUN) concentrations in the serum and malondialdehyde (MDA) in the liver and renal tissues were higher than the control (all P<0.001). All doses of Vit C increased thyroxine, protein and albumin and thiol content in in renal and liver tissues while, decreased AST, ALT and ALK-P concentration and MDA in liver and renal tissues compared to PTU group (P<0.05–P<0.001). Creatinine, BUN and SOD and CAT were improved by both 100 and 500 mg/kg of Vit C in the renal (P<0.05–P<0.001) and by 100 mg/kg in the liver (P<0.05–P<0.001). Conclusion: Vit C improved liver and renal function of hypothyroid rats which might be due to its protective effects against tissues oxidative damage.
DOAJ (DOAJ: Directory of Open Access Journals), Jul 28, 2016
Objective: In this study, we aimed at evaluation of the efficacy of Hypericum perforatum and flax... more Objective: In this study, we aimed at evaluation of the efficacy of Hypericum perforatum and flaxseed on hot flash, vaginal atrophy and estrogen-dependent cancers in menopausal women Materials and Methods: We searched MEDLINE, Scopus, and the Cochrane Central Register of Controlled Trials (RCT) to explore trials that assessed the effectiveness of H. perforatum and flaxseed on hot flash, vaginal atrophy and estrogen-dependent cancers. In this regard, the following terms were used "menopause AND H. perforatum OR flaxseed OR Linum usitatissimum. Only randomized controlled trials were included in the study. Results: Nine RCTs were included in this systematic review. Based on the literature, flaxseed showed beneficial effect on hot flash frequency and intensity, which was not statistically significant. According to two trials, flaxseed showed estrogenic effects; however, no conclusion regarding cancer promoting or protecting effects can be made. The evidence of the efficacy of the flaxseed on alleviating vaginal atrophy was also limited due to inconsistent findings in this regard. One trial declared that Vitex agnus-castus and H. perforatum showed comparable decrease in the frequency of hot flashes. Conclusion: The results of our systematic review suggest beneficial effect on vasomotor symptom with both of flaxseed and H. perforatum. Consistent conclusion regarding estrogendependent cancers and maturation value is limited due to small number of trials related to flaxseed. Further trials are still needed to confirm the results of our systematic review.
Journal of Bodywork and Movement Therapies, Jul 1, 2016
Exercise-induced asthma is seen following vigorous or prolonged exercise or phys ical exertion. I... more Exercise-induced asthma is seen following vigorous or prolonged exercise or phys ical exertion. It has been suggested that climatic conditions have an influence on exerciseinduced asthma. Therefore, the aim of the present study was to examine the effect of two cli matic conditions on exercise-induced deterioration of pulmonary function tests in 10e12 year old students. Two hundred and fifty six students were randomly chosen from two cities namely Kerman and Gorgan (128 subjects in each who were equally from both cities) including 62 girls and 66 boys of 10e12 years old. A questionnaire was used to obtain demographic information and to identify the prevalence of asthma symptoms. Each subject performed a sevenminute free run exercise with maximum effort and sufficient motivation until they reached 70e75% heart rate. Pulmonary function tests (PFT) including, forced vital capacity (FVC), forced expiratory volume in one second (FEV 1), peak expiratory flow (PEF), and maximum expi ratory flow at 50% of vital capacity (MEF 50
shiraz e medical journal, Nov 29, 2017
Background: Cyclophosphamide (CP) is one of the most popular bifunctional alkylating agents, whic... more Background: Cyclophosphamide (CP) is one of the most popular bifunctional alkylating agents, which is utilized in the treatment of numerous cancer diseases and has toxic side effects such as nephrotoxicity, hematotoxicity, mutagenicity, and immunotoxicity. In this study, Elaeagnus angustifolia fruit extract (EAFE), a natural plant extract, was examined for its antioxidant activity against CP-induced renal oxidative damage in mice. Methods: In this experimental study, 30 male Swiss albino mice (25-30 g) were separated into five groups. Group 1 received normal saline for 5 days; group 2 was treated with CP 200 mg/kg single intraperitoneal (i.p) dose only on the 5th day; groups 3 to 5 received EAFE at doses of 100, 200, and 400 mg/kg, respectively, administered orally during 5 days, and CP on the 5th day 1 hour after the last dose of extract administration. Then on the 6th day, the mice were sacrificed. Blood samples were collected to determine serum blood urea nitrogen (BUN) and creatinine (Cr) levels. Levels of malondialdehyde (MDA) and glutathione (GSH) in kidney tissue were also examined. Histological assessment was undertaken on kidney samples taken from all groups. Results: The results obtained from our study showed a significant increase in the levels of MDA, Cr, and BUN and a decrease in GSH following CP injection. Pre-treatment with EAFE showed a decrease in the levels of MDA, Cr, and BUN and an increase in GSH in all doses; however, the most significant reduction was observed at doses of 200 and 400 mg/kg. In addition, the nephroprotective effect of EAFE was confirmed by histological evaluation of the kidneys. Conclusions: Our results indicate that EAFE has protective effects against CP-induced renal damage possibly by amelioration of biochemical indices and oxidative stress parameters.
DOAJ (DOAJ: Directory of Open Access Journals), Oct 16, 2015
Objective: Oxidative stress damages cells and brings about the pathogenesis of ischemia/reperfusi... more Objective: Oxidative stress damages cells and brings about the pathogenesis of ischemia/reperfusion injury. This study was carried out to investigate the preconditioning and cardio protective potential effects of crocin and vitamin E by the eNOS and iNOS express gene in ischemia/reperfusion in rats. Material & Methods: Male rats were divided into seven groups, namely: sham, control group and experimental groups treated with crocin(10, 20 and 40 mg/kg), vitamin E (100 mg/kg) and combination of crocin (40 mg/kg) with vitamin E (100 mg/kg) that were gavaged The heart was removed and relocated to a Langendorff apparatus and subjected to global ischemia and then the left ventricular end diastolic pressure (LVEDP) were measured as a hemodynamic parameter. Total RNA was extracted from heart frozen tissues. RT-PCR technique was performed by specific primers designed for nitric oxide gene and the results were assessed by agarose gel electrophoresis. Results: Results after ischemia and reperfusion showed that crocin 40 mg/kg produced a significant improvement of LVEDP as a mechanical function (p<0.05), associated with a reduction of iNOS release (p<0.05). The eNOS mRNA levels were significantly higher in crocin-treated 40 mg/kg compared to controls treated by RT-PCR technique. The combination of crocin and vitamin E have shown more effective on the reduction of iNOS release (p<0.01). Conclusion: In the isolated rat heart, protective effect of crocin, may possibly be explained by regulating eNOS and iNOS expressions. The Results resultsconfirmed the hypothesis that cardioprotective effect of crocin is partly mediated by nitric oxide. This could explain the cardioprotective action of crocin following ischemia and reperfusion.
Research in Pharmaceutical Sciences, 2019
Previous studies have shown that some plants in the genus of Ferula (Apiaceae) have antidiabetic ... more Previous studies have shown that some plants in the genus of Ferula (Apiaceae) have antidiabetic effects. The present work was aimed to evaluate effects of Ferula gummosa oleo-resin in a rat model of streptozotocin-induced diabetes. Male Wistar rats were randomized into five groups (n = 6): normal control, diabetic control, diabetic rats treated with insulin (3 IU/day), and diabetic rats treated with 100 or 400 mg/kg/day of an ethanolic extract of the oleo-resin. After 4 weeks, blood samples were collected for measuring fasting blood glucose (FBG), lipid profile, aspartate aminotransferase, alanine aminotransferase (ALT), alkaline phosphatase, blood urea nitrogen, and creatinine. In addition, levels of lipid peroxidation, thiol groups, and superoxide dismutase (SOD) activity were evaluated in the liver and kidney. At the end of the fourth week, the level of FBG in rats treated with 100 mg/kg of the extract was lower than that in diabetic control rats (273 ± 39 mg/dL vs 471 ± 32 mg/dL). Administration of insulin and the extract had no significant effects on the serum lipids. Insulin and both doses of the extract significantly reduced the activity of ALT. In addition, the extract inhibited lipid peroxidation in the kidney and restored the elevated level of SOD in the liver and kidneys. Ferula gummosa oleo-resin has the potential to prevent or delay the complications of diabetes by inhibiting the progression of hyperglycemia and attenuating oxidative stressinduced damage in the liver and kidneys.
Jundishapur Scientific Medical Journal, Nov 21, 2012
Biomedicine & Pharmacotherapy
Elsevier Masson, Dec 31, 2017
Effect of crocin on nitric oxide synthase expression in post-ischemic isolated rat heart
Avicenna Journal of Phytomedicine, 2016
Objective: In this study, we aimed at evaluation of the efficacy of Hypericum perforatum and flax... more Objective: In this study, we aimed at evaluation of the efficacy of Hypericum perforatum and flaxseed on hot flash, vaginal atrophy and estrogen-dependent cancers in menopausal women Materials and Methods: We searched MEDLINE, Scopus, and the Cochrane Central Register of Controlled Trials (RCT) to explore trials that assessed the effectiveness of H. perforatum and flaxseed on hot flash, vaginal atrophy and estrogen-dependent cancers. In this regard, the following terms were used “menopause AND H. perforatum OR flaxseed OR Linum usitatissimum. Only randomized controlled trials were included in the study. Results: Nine RCTs were included in this systematic review. Based on the literature, flaxseed showed beneficial effect on hot flash frequency and intensity, which was not statistically significant. According to two trials, flaxseed showed estrogenic effects; however, no conclusion regarding cancer promoting or protecting effects can be made. The evidence of the efficacy of the flaxse...
[
a Department of Exercise Physiology, Faculty of Physical Education & Sport Sciences, Shahid Bahon... more a Department of Exercise Physiology, Faculty of Physical Education & Sport Sciences, Shahid Bahonar University of Kerman, Kerman, Iran b Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran c Neonatal and Children’s Health Research Center, Golestan University of Medical Sciences, Gorgan, Iran d Student Research Committee, Esfarayen Faculty of Medical Sciences, Esfarayen, Iran e Neurogenic Inflammation Research Centre and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Background and Objective: Bleeding from esophageal varicose (EV) is one of the lethal complicatio... more Background and Objective: Bleeding from esophageal varicose (EV) is one of the lethal complications of liver cirrhosis. Upper gastro-intestinal endoscopy (UGIE) is performed in all cirrhotic patients for screening EV but recently some non-invasive methods have been evaluated as a substitute. For this purpose the aim of this study was to assess the correlation between EV and reno-vascular resistance index (RRI) in cirrhotic patients. Subjects and Methods: Sixty four cirrhotic patients with normal renal function were included and underwent UGIE and measuring RRI using color flow and pulsed wave Doppler ultrasonography. Correlation between the mean of RRI was evaluated with presence and grade of EV. P<0.05 was considered statistically significant. Results: EV was detected in 42 patients (65.5%). The means of RRI were 0.68±0.03 versus 0.62±0.04 in patients with or without EV, respectively (P<0.001). Moreover, there was a significant statistically difference between the mean of ...
Background and Objective: Ulcerative colitis is one of the inflammatory bowel diseases with unkno... more Background and Objective: Ulcerative colitis is one of the inflammatory bowel diseases with unknown etiology. Studies done in different parts of the world showed that the sites of colonic involvement and epidemiology of this disease are varied. The aim of this study was to investigate the pattern of colonic involvement and epidemiologic charateristics of patient referred to Imam Khomeini hospital of Ahvaz during the years 2007-2011. Subjects and Methods: In this retrospective study, the data from 98 patients, who referred to Imam Khomeini hospital during these 5 years with ulcerative colitis, which was confirmed by colonoscopy or pathology, were investigated and analyzed. Results: Mean age of patients was 38.95±2.14 years, 42.8 % were in age group of 31-50 years and male to female ratio was 1.18. The most frequent site of colonic involvement was rectosigmoid (21.4 %) and pancolit (19.4 %). Although 52 % of patients had no co-existing disease, anemia was the most prevalent co-existin...
Avicenna Journal of Phytomedicine, 2015
Objective: Oxidative stress damages cells and brings about the pathogenesis of ischemia/reperfusi... more Objective: Oxidative stress damages cells and brings about the pathogenesis of ischemia/reperfusion injury. This study was carried out to investigate the preconditioning and cardio protective potential effects of crocin and vitamin E by the eNOS and iNOS express gene in ischemia/reperfusion in rats. Material & Methods: Male rats were divided into seven groups, namely: sham, control group and experimental groups treated with crocin(10, 20 and 40 mg/kg), vitamin E (100 mg/kg) and combination of crocin (40 mg/kg) with vitamin E (100 mg/kg) that were gavaged The heart was removed and relocated to a Langendorff apparatus and subjected to global ischemia and then the left ventricular end diastolic pressure (LVEDP) were measured as a hemodynamic parameter. Total RNA was extracted from heart frozen tissues. RT-PCR technique was performed by specific primers designed for nitric oxide gene and the results were assessed by agarose gel electrophoresis. Results: Results after ischemia and reperf...
Journal of Mazandaran University of Medical Sciences, 2019
Background and purpose: Occlusion is a decreasing factor on longevity of peripheral intravenous c... more Background and purpose: Occlusion is a decreasing factor on longevity of peripheral intravenous catheters (PIVCs). Continuous flushing (injection of specific volume of solution such as Normal Saline (N/S) before and after each drug infusion into PIVCs at determined interval time) is one of the effective methods in in vitro studies to maintain the PIVCs patency. The aim of this study was to investigate the effect of continuous flushing on the duration of PIVCs patency. Materials and methods: In a randomized controlled trail, 72 patients hospitalized in medical/surgical department in Esfarayen Imam Khomeini Hospital, north east of Iran, were randomly assigned into either an intervention group or a control group. In intervention group, 5 ml of N/S solution was injected within 5 seconds before and after injection of any drug into the PIVCs. If another drug was administered at the same time, it was injected and flushing technique was performed again. The PIVCs patency was monitored every...
Shiraz E-Medical Journal, 2017
Background: Cyclophosphamide (CP) is one of the most popular bifunctional alkylating agents, whic... more Background: Cyclophosphamide (CP) is one of the most popular bifunctional alkylating agents, which is utilized in the treatment of numerous cancer diseases and has toxic side effects such as nephrotoxicity, hematotoxicity, mutagenicity, and immunotoxicity. In this study, Elaeagnus angustifolia fruit extract (EAFE), a natural plant extract, was examined for its antioxidant activity against CP-induced renal oxidative damage in mice. Methods: In this experimental study, 30 male Swiss albino mice (25-30 g) were separated into five groups. Group 1 received normal saline for 5 days; group 2 was treated with CP 200 mg/kg single intraperitoneal (i.p) dose only on the 5th day; groups 3 to 5 received EAFE at doses of 100, 200, and 400 mg/kg, respectively, administered orally during 5 days, and CP on the 5th day 1 hour after the last dose of extract administration. Then on the 6th day, the mice were sacrificed. Blood samples were collected to determine serum blood urea nitrogen (BUN) and creatinine (Cr) levels. Levels of malondialdehyde (MDA) and glutathione (GSH) in kidney tissue were also examined. Histological assessment was undertaken on kidney samples taken from all groups. Results: The results obtained from our study showed a significant increase in the levels of MDA, Cr, and BUN and a decrease in GSH following CP injection. Pre-treatment with EAFE showed a decrease in the levels of MDA, Cr, and BUN and an increase in GSH in all doses; however, the most significant reduction was observed at doses of 200 and 400 mg/kg. In addition, the nephroprotective effect of EAFE was confirmed by histological evaluation of the kidneys. Conclusions: Our results indicate that EAFE has protective effects against CP-induced renal damage possibly by amelioration of biochemical indices and oxidative stress parameters.
Journal of Nephropathology, 2017
Background: Cyclophosphamide (CP) is amongst the most effective alkylating anticancer drugs which... more Background: Cyclophosphamide (CP) is amongst the most effective alkylating anticancer drugs which regardless of its harmful side effects including nephrotoxicity, hematotoxicity, mutagenicity and also immunotoxicity, commonly raised for the treatment of a number of cancers and autoimmune ailments. Objectives: This study was undertaken to investigate the ameliorative effect of Lavandula officinalis L extract (LOE) in nephrotoxicity induced by CP in mice. Materials and Methods: In this experimental study, 35 male Swiss albino mice (20-25 g) were randomly divided into 5 groups, each group consist of 7 mice. Mice were pretreated with LOE orally in doses of 100, 200 and 400 mg/kg for 5 consecutive days and CP (200 mg/kg, ip) was administrated on the fifth day 1 hour after the last dose of extract. Then on the sixth day, animals were sacrificed. Blood samples were collected to determine serum creatinine (Cr) and blood urea nitrogen (BUN) levels. Malondialdehyde (MDA), glutathione (GSH) levels and catalase (CAT) and superoxide dismutase (SOD) activity were assayed in kidney tissue. The kidney was maintained in formalin for histological examination. Results: Results showed a significant increase in the levels of MDA, Cr and BUN, and decrease of GSH, CAT and SOD by CP administration. Pre-treatment with LOE showed reduction in the levels of MDA, Cr and BUN and increase of GSH, CAT and SOD in all doses but the most significant alteration was observed at doses of 200 and 400 mg/ kg (P < 0.05). Additionally the nephroprotective effect of the LOE was established by the histological examination of the kidneys. Conclusions: Our results indicated that LOE has produced amelioration in biochemical indices and oxidative stress parameters against CP-induced nephrotoxicity.
Research in Pharmaceutical Sciences, 2019
Previous studies have shown that some plants in the genus of Ferula (Apiaceae) have antidiabetic ... more Previous studies have shown that some plants in the genus of Ferula (Apiaceae) have antidiabetic effects. The present work was aimed to evaluate effects of Ferula gummosa oleo-resin in a rat model of streptozotocin-induced diabetes. Male Wistar rats were randomized into five groups (n = 6): normal control, diabetic control, diabetic rats treated with insulin (3 IU/day), and diabetic rats treated with 100 or 400 mg/kg/day of an ethanolic extract of the oleo-resin. After 4 weeks, blood samples were collected for measuring fasting blood glucose (FBG), lipid profile, aspartate aminotransferase, alanine aminotransferase (ALT), alkaline phosphatase, blood urea nitrogen, and creatinine. In addition, levels of lipid peroxidation, thiol groups, and superoxide dismutase (SOD) activity were evaluated in the liver and kidney. At the end of the fourth week, the level of FBG in rats treated with 100 mg/kg of the extract was lower than that in diabetic control rats (273 ± 39 mg/dL vs 471 ± 32 mg/dL). Administration of insulin and the extract had no significant effects on the serum lipids. Insulin and both doses of the extract significantly reduced the activity of ALT. In addition, the extract inhibited lipid peroxidation in the kidney and restored the elevated level of SOD in the liver and kidneys. Ferula gummosa oleo-resin has the potential to prevent or delay the complications of diabetes by inhibiting the progression of hyperglycemia and attenuating oxidative stressinduced damage in the liver and kidneys.
International Journal for Vitamin and Nutrition Research, 2019
. Background: The effects of Vit C on liver and renal function and the tissues oxidative damage w... more . Background: The effects of Vit C on liver and renal function and the tissues oxidative damage was investigated in hypothyroid rats. Materials and methods: The pregnant rats were divided into 5 groups (n=6): (1) Control; (2) Propylthiouracil (PTU; 0.005%), (3–5) PTU plus 10, 100 or 500 mg/kg b.w. Vit C. The drugs were added to the drinking water of the dams and their pups during lactation period and then continued for the offspring through the first 8 weeks of their life. Finally, 7 male offspring from each group were randomly selected. Results: Thyroxine, protein and albumin concentrations in the serum and thiol content and superoxide dismutase (SOD) and catalase (CAT) activities in renal and liver tissues of hypothyroid group was lower (all P<0.001) while, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALK-P), creatinine and blood urea nitrogen (BUN) concentrations in the serum and malondialdehyde (MDA) in the liver and renal tissues were higher than the control (all P<0.001). All doses of Vit C increased thyroxine, protein and albumin and thiol content in in renal and liver tissues while, decreased AST, ALT and ALK-P concentration and MDA in liver and renal tissues compared to PTU group (P<0.05–P<0.001). Creatinine, BUN and SOD and CAT were improved by both 100 and 500 mg/kg of Vit C in the renal (P<0.05–P<0.001) and by 100 mg/kg in the liver (P<0.05–P<0.001). Conclusion: Vit C improved liver and renal function of hypothyroid rats which might be due to its protective effects against tissues oxidative damage.