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Papers by fatima mahmoudi

Research paper thumbnail of Characteristic and physiological properties of Bifidobacterium strains isolated from different origin

Biological systems : open access, Aug 17, 2015

Research paper thumbnail of Safety and Protective Effect of Bifidobactérium Spp. Used as Probiotic Agent in Vivo Against Enteropathogenic Escherichia Coli

Innovative Romanian Food Biotechnology, 2015

This study showed the effect of fermented milk with Bifidobacterium bifidum on the contaminations... more This study showed the effect of fermented milk with Bifidobacterium bifidum on the contaminations by Escherichia coli enteropathogenic EPEC and intestinal disorders. The B. bifidum strain (Bf) was isolated on MRS medium containing 0.5g/L of cysteine hydrochloride and 2 mg/L of nalidixic acid. This strain was isolated from breast- fed infant feces. This strain is considered as a model strain, for antagonism in vivo developed in the rat (wistar). It has has been demonstrated that the administration of Bifidobacterium strain cause a significant reduction of the rate of E. coli enteropathogenic multiplication in the rats feces. Results of the macroscopic study of histological sections have confirmed the positive effect of treatment with bifidobacteria. In this study, the effect of Bifidobacterium addition in reducing the enteropathogenic bacteria effect in the digestive tract was confirmed by in vitro and in vivo conditions.

Research paper thumbnail of Identification and Physiological Properties of Bifidobactérium Strains Isolated from Different Origin

Bifidobacteria play an important role in maintaining human health, and suppress harmful bacteria ... more Bifidobacteria play an important role in maintaining human health, and suppress harmful bacteria by controlling the pH of the large intestine. About 14 strains of the Bifidobacteria have been isolated from the faeces of infants nursed exclusively from the maternal milk (their ages less than 5 months) from commercial French yoghurt (active bifidus) and from milk (celia bifidus) on MRS medium (containing 0.05% cysteine-HCl, with nalidixic acid 2 mg m -1 ). A total of six strain are strict anaerobic and gram-positive Bifidobacteria, identified as, B. longum (60% of the samples) was the most frequently found species, followed by B. bifidum (21.0%), B. breve (19%). These strains were evaluated for potential use as probiotics based on their adhesion to intestinal epithelial cells, resistance towards acidic (resistance to low pH) and bile conditions. pH values ranging from 6.5 to 7, however, these strains are sensitive to the pH 8, Bifidobacterium are mesophiles with optimal growth at 36 °...

Research paper thumbnail of Evaluation of in vitro antagonism and protection against enteropathogenic experimental challenge of different strains of Bifidobacterium

Gastrointestinal microflora highly impacts their host mainly by performing a great variety of met... more Gastrointestinal microflora highly impacts their host mainly by performing a great variety of metabolic activities, protecting the host from pathogenic colonization. Mother's milk is a prebiotic factor which stimulates bifidobacteria growth in vivo. All strains of bifidobacteria were isolated on MRS medium (in addition to 0.05% cysteine HCL and 2 mg/l of nalidixic acid) from different origins (breast-fed infant faeces and yoghurt (bifidus). The strains belong to the following species: Bifidobacterium longum, B. Breve, B. bifidum. We studied the antagonist power of Bifidobacterium against enteropathogenes (S. aureus, Escherichia coli, P. aeruginosa, Salmonella. Sp), using agar diffusion method. In vitro antagonism test showed that our strains were able to produce antagonistic substances against various pathogenic microorganisms. The activity was completely destroyed by the action of proteolytic enzymes, indicating that the biologically active portion is proteinaceous. These prope...

Research paper thumbnail of Safety and Protective Effect of Bifidobactérium Spp. Used as Probiotic Agent in Vivo Against Enteropathogenic Escherichia Coli

Research paper thumbnail of Antimicrobial substances produced by bifidobacterium and its effect on enteropathogenic bacteria

Research paper thumbnail of African Journal of Microbiology Research Evaluation of in vitro antagonism and protection against enteropathogenic experimental challenge of different strains of Bifidobacterium

Research paper thumbnail of Identification and Physiological Properties of Bifidobactérium Strains Isolated from Different Origin

Bifidobacteria play an important role in maintaining human health, and suppress harmful bacteria ... more Bifidobacteria play an important role in maintaining human health, and suppress harmful bacteria by controlling the pH of the large intestine. About 14 strains of the Bifidobacteria have been isolated from the faeces of infants nursed exclusively from the maternal milk (their ages less than 5 months) from commercial French yoghurt (active bifidus] and from milk (celia bifidus) on MRS medium (containing 0.05% cysteine-HCl, with nalidixic acid 2 mg m-1). A total of six strain are strict anaerobic and gram-positive Bifidobacteria, identified as, B. longum (60% of the samples) was the most frequently found species, followed by B. bifidum (21.0%), B. breve (19%). These strains were evaluated for potential use as probiotics based on their adhesion to intestinal epithelial cells, resistance towards acidic (resistance to low pH) and bile conditions. pH values ranging from 6.5 to 7, however, these strains are sensitive to the pH 8, Bifidobacterium are mésophiles with optimal growth at 36 °C ...

Research paper thumbnail of Characteristic and physiological properties of Bifidobacterium strains isolated from different origin

Biological systems : open access, Aug 17, 2015

Research paper thumbnail of Safety and Protective Effect of Bifidobactérium Spp. Used as Probiotic Agent in Vivo Against Enteropathogenic Escherichia Coli

Innovative Romanian Food Biotechnology, 2015

This study showed the effect of fermented milk with Bifidobacterium bifidum on the contaminations... more This study showed the effect of fermented milk with Bifidobacterium bifidum on the contaminations by Escherichia coli enteropathogenic EPEC and intestinal disorders. The B. bifidum strain (Bf) was isolated on MRS medium containing 0.5g/L of cysteine hydrochloride and 2 mg/L of nalidixic acid. This strain was isolated from breast- fed infant feces. This strain is considered as a model strain, for antagonism in vivo developed in the rat (wistar). It has has been demonstrated that the administration of Bifidobacterium strain cause a significant reduction of the rate of E. coli enteropathogenic multiplication in the rats feces. Results of the macroscopic study of histological sections have confirmed the positive effect of treatment with bifidobacteria. In this study, the effect of Bifidobacterium addition in reducing the enteropathogenic bacteria effect in the digestive tract was confirmed by in vitro and in vivo conditions.

Research paper thumbnail of Identification and Physiological Properties of Bifidobactérium Strains Isolated from Different Origin

Bifidobacteria play an important role in maintaining human health, and suppress harmful bacteria ... more Bifidobacteria play an important role in maintaining human health, and suppress harmful bacteria by controlling the pH of the large intestine. About 14 strains of the Bifidobacteria have been isolated from the faeces of infants nursed exclusively from the maternal milk (their ages less than 5 months) from commercial French yoghurt (active bifidus) and from milk (celia bifidus) on MRS medium (containing 0.05% cysteine-HCl, with nalidixic acid 2 mg m -1 ). A total of six strain are strict anaerobic and gram-positive Bifidobacteria, identified as, B. longum (60% of the samples) was the most frequently found species, followed by B. bifidum (21.0%), B. breve (19%). These strains were evaluated for potential use as probiotics based on their adhesion to intestinal epithelial cells, resistance towards acidic (resistance to low pH) and bile conditions. pH values ranging from 6.5 to 7, however, these strains are sensitive to the pH 8, Bifidobacterium are mesophiles with optimal growth at 36 °...

Research paper thumbnail of Evaluation of in vitro antagonism and protection against enteropathogenic experimental challenge of different strains of Bifidobacterium

Gastrointestinal microflora highly impacts their host mainly by performing a great variety of met... more Gastrointestinal microflora highly impacts their host mainly by performing a great variety of metabolic activities, protecting the host from pathogenic colonization. Mother's milk is a prebiotic factor which stimulates bifidobacteria growth in vivo. All strains of bifidobacteria were isolated on MRS medium (in addition to 0.05% cysteine HCL and 2 mg/l of nalidixic acid) from different origins (breast-fed infant faeces and yoghurt (bifidus). The strains belong to the following species: Bifidobacterium longum, B. Breve, B. bifidum. We studied the antagonist power of Bifidobacterium against enteropathogenes (S. aureus, Escherichia coli, P. aeruginosa, Salmonella. Sp), using agar diffusion method. In vitro antagonism test showed that our strains were able to produce antagonistic substances against various pathogenic microorganisms. The activity was completely destroyed by the action of proteolytic enzymes, indicating that the biologically active portion is proteinaceous. These prope...

Research paper thumbnail of Safety and Protective Effect of Bifidobactérium Spp. Used as Probiotic Agent in Vivo Against Enteropathogenic Escherichia Coli

Research paper thumbnail of Antimicrobial substances produced by bifidobacterium and its effect on enteropathogenic bacteria

Research paper thumbnail of African Journal of Microbiology Research Evaluation of in vitro antagonism and protection against enteropathogenic experimental challenge of different strains of Bifidobacterium

Research paper thumbnail of Identification and Physiological Properties of Bifidobactérium Strains Isolated from Different Origin

Bifidobacteria play an important role in maintaining human health, and suppress harmful bacteria ... more Bifidobacteria play an important role in maintaining human health, and suppress harmful bacteria by controlling the pH of the large intestine. About 14 strains of the Bifidobacteria have been isolated from the faeces of infants nursed exclusively from the maternal milk (their ages less than 5 months) from commercial French yoghurt (active bifidus] and from milk (celia bifidus) on MRS medium (containing 0.05% cysteine-HCl, with nalidixic acid 2 mg m-1). A total of six strain are strict anaerobic and gram-positive Bifidobacteria, identified as, B. longum (60% of the samples) was the most frequently found species, followed by B. bifidum (21.0%), B. breve (19%). These strains were evaluated for potential use as probiotics based on their adhesion to intestinal epithelial cells, resistance towards acidic (resistance to low pH) and bile conditions. pH values ranging from 6.5 to 7, however, these strains are sensitive to the pH 8, Bifidobacterium are mésophiles with optimal growth at 36 °C ...