mahmoud rashed - Academia.edu (original) (raw)

Papers by mahmoud rashed

Characterization of different Lactobacillus bacterial strains has an increasing demand due to the... more Characterization of different Lactobacillus bacterial strains has an increasing demand due to their potential health benefits. Classical cell counting methods are time-consuming and require well trained laboratory work, besides they are having higher subjectivity and error. The determination of electrical impedance spectrum is a promising method for the evaluation of changes in chemical structure and cell growth during the formation of yoghurt. Our aim is to use electrical impedance spectrum for the monitoring of cell growth of LABs. In this study different strains of Lactobacillus bulgaricus bacteria were used for the production of yoghurt from ultrahigh temperature processed (UHT) milk of 1.5% fat content. The cell count of the LABs was determined with plating method on MRS (De Man, Rogosa and Sharpe) agar in each hour during 12 h of cultivation at 37 °C. For impedance measurements, the real part (conductance) and the imaginary part (susceptance) of admittance (reciprocal value of...

Background: One of the frequent musculoskeletal problems is knee pain. The trend of increasing of... more Background: One of the frequent musculoskeletal problems is knee pain. The trend of increasing of knee pain among the populations is noted. Therefore, choosing a reliable screening tool with reasonable cost is mandatory. Although magnetic resonance imaging (MRI) is the gold standard imaging modality for knee soft tissue structures, it has been widely abused with its high cost. Ultrasound is an established modality to image the soft tissue structures of the knee. Objective: To show the role of ultrasound (US) in evaluating of knee pain and comparing the results with magnetic resonance imaging (MRI). Patients and methods: This study included 40 patients (18 males and 22 females) with an age ranging from 15-69 years. This study was carried out at the Radiology Department of Al-Azhar University Hospitals for ultrasonography and MRI examination during the period from October 2019 to November 2020. Results: Regarding meniscal horn tear, ultrasound detected tear in 2 cases, while MRI detec...

Innovation Strategies in the Food Industry

Sensors

Honey is produced by honeybees and is used as a food and medical product. Adulteration of honey h... more Honey is produced by honeybees and is used as a food and medical product. Adulteration of honey has been a problem for several years now because of the relatively high price of honey on the market according to its valuable composition. The aim of our study is to determine the physicochemical properties of authentic Hungarian linden and acacia honeys (pure samples or manipulated ones blended with sugar syrup) as well as commercially available blends of European Union (EU) non-European Union (non-EU) honeys. Authentic linden and acacia were blended with sugar syrup at 10%, 20% and 50% concentration levels, and physicochemical properties were determined according to the methods of the International Honey Commission. Our objectives also included testing of the performance of electronic sensory techniques (electronic tongue (ET) and electronic nose (EN)) in the detection of adulteration, and the results are compared to the sensory profile analysis. The results provide good average recogn...

Frontiers in Pharmacology

Ectonucleoside triphosphate diphosphohydrolases (NTPDases) catalyze the hydrolysis of nucleoside ... more Ectonucleoside triphosphate diphosphohydrolases (NTPDases) catalyze the hydrolysis of nucleoside tri- and di-phosphates to mono-phosphates. The products are subsequently hydrolyzed by ecto-5′-nucleotidase (ecto-5′-NT) to nucleosides. NTPDase inhibitors have potential as novel drugs, e.g., for the treatment of inflammation, neurodegenerative diseases, and cancer. In this context, a series of anthraquinone derivatives structurally related to the anthraquinone dye reactive blue-2 (RB-2) was synthesized and evaluated as inhibitors of human NTPDases utilizing a malachite green assay. We identified several potent and selective inhibitors of human NTPDase2 and -3. Among the most potent NTPDase2 inhibitors were 1-amino-4-(9-phenanthrylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate (20, PSB-16131, IC50 of 539 nM) and 1-amino-4-(3-chloro-4-phenylsulfanyl)phenylamino-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate (48, PSB-2020, IC50 of 551 nM). The most potent NTPDase3 inhibitors were 1-amino-4-[3-(4,6-dichlorotriazin-2-ylamino)-4-sulfophenylamino]-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate (42, PSB-1011, IC50 of 390 nM) and 1-amino-4-(3-carboxy-4-hydroxyphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate (33, PSB-2046, IC50 of 723 nM). The best NTPDase2 inhibitor 20 showed a non-competitive inhibition type, while the NTPDase3 inhibitor 42 behaved as a mixed-type inhibitor. These potent compounds were found to be selective vs. other NTPDases. They will be useful tools for studying the roles of NTPDase2 and -3 in physiology and under pathological conditions.

Bioorganic Chemistry

Inhibiting VEGFR-2 has been set up as a therapeutic strategy for treatment of cancer. Accordingly... more Inhibiting VEGFR-2 has been set up as a therapeutic strategy for treatment of cancer. Accordingly, new quinazoline-based derivatives having the structural features of VEGFR-2 inhibitors were designed and synthesized. Anti-proliferative activities were evaluated against three human cancer cell lines (HepG-2, MCF-7 and HCT-116) using MTT assay method. Doxorubicin and sorafenib were used as positive controls. Compounds 26b, 29a, 29b and 30 showed excellent anti-cancer activities against all cell lines. Moreover, compound 31 was the most active with IC 50 values of 3.97 ± 0.2, 4.83 ± 0.2 and 4.58 ± 0.3 µM, respectively. The most active cytotoxic agents were further evaluated in vitro for their VEGFR-2 inhibitory activities, compound 31 showed a high activity against VEGFR-2 with an IC50 value of 2.5 ± 0.04 µM, almost equal to that of sorafenib (IC50 = 2.4 ± 0.05 µM). Further studies revealed the ability of this promising quinazoline derivative 31 to induce apoptosis and arrest cell cycle growth at G2/M phase. In vivo antitumor activities of the synthesized compounds revealed that compounds 30 and 31 possessed significant tumor growth inhibition effect. Molecular docking studies were also performed and finally we can say that VEGFR-2 inhibition confers the reported cytotoxic activities.

The Journal of the World Universities Forum

Progress in Agricultural Engineering Sciences

People have recently started to pay more attention to the healthier lifestyle, which also include... more People have recently started to pay more attention to the healthier lifestyle, which also includes the consumption of more natural and less processed food products. Honey as one of the most often used natural sweeteners has also been reconsidered and more commonly used. However, honey has also been the target of food adulteration due to its emerging use and relatively high price. Therefore, there is an increasing need to develop rapid evaluation methods for the identification of honey from different sources. Experiments have been performed with 79 authentic honey samples of different floral and geographical origins, mainly from Hungary. The standard analytical parameters used to characterize the nutritional values of honey such as antioxidant capacity, polyphenol content, ash content, pH, conductivity have been determined. The samples were also analyzed with a benchtop near infrared (NIR) spectrometer to record their NIR spectra. The data acquired with NIR spectroscopy measurements ...

Progress in Agricultural Engineering Sciences

The study focused on the efficacy of ultrasonic method for identifying vegetable oils and their m... more The study focused on the efficacy of ultrasonic method for identifying vegetable oils and their mixtures in the formulation of frying oil and its ability in authentication of virgin olive oil. The ultrasonic propagation properties (velocity and Time of Flight (TOF)) were used to classify oil samples and their mixtures at 1 MHz. The results revealed the ability to classify oil types in terms of their level of un-saturation, besides it is to identify oil mixtures. Each oil sample could be grouped into different clusters using ultrasonic parameters. Hence, ultrasonic could be used to discriminate the vegetable oil types and their mixtures effectively as a rapid and continuous method in the industrial in-line quality control system of vegetable oils and their mixtures.

Honey has been widely used for health care and as sweetener since the ancient times. Due to its g... more Honey has been widely used for health care and as sweetener since the ancient times. Due to its great nutritional value and its high price, honey is one of the most adulterated products on the market. Therefore, there is a need to develop new quick measurement methods to recognize the adulteration. Almost 80 authentic honey samples of different floral and geographical origins were collected for our experiments, focusing mainly on Hungarian honey. Various analytical methods were used for the determination of the nutritional values of the honey samples, e.g. antioxidant capacity, polyphenol content, ash content, pH, conductivity, etc. These measurements aim to complete the scarcely available data on Hungarian honeys. In addition, we determined sensory properties by color and electronic tongue analyses. Electronic tongue enables easy sample preparation and results are delivered in a short time. Evaluating the results by different multivariate statistical methods, determination of the f...

The Egyptian Journal of Radiology and Nuclear Medicine, 2016

Abstract Objective Compare CT perfusion between responders and non-responders in patients with re... more Abstract Objective Compare CT perfusion between responders and non-responders in patients with residual cervical nodes after lymphoma treatment. Subjects and methods Twenty-seven patients with treated pathologically proved lymphoma were included in this prospective study, divided into two groups: responders (n. 15) and non-responders to treatment (n. 12). Blood flow (BF), blood volume (BV), mean transit time (MTT) and permeability (PS) were determined using CT perfusion. Lymph nodes sizes were measured (short axis). Results BF, BV and PS were significantly higher in non-responders group [93.3 (65.3–111) ml/min/100 g, 6.3 (5.2–8) ml/100 g and 18.9 (14.5–25.1) ml/min/100 g respectively] as compared to responders group [54 (43.8–77.5) ml/min/100 g, 4.1 (2.2–4.8) ml/100 g and 9.4 (7.5–14.5) ml/min/100 g respectively], [ p 0.019, 0.004 and 0.004 respectively]. MTT had a trend to be low in non-responders group, but did not reach statistical significance ( p 0.305). Cutoff value for BF, BV and PS was 59.5 ml/min/100 g, 3.6 ml/100 g and 11.1 ml/min/100 g respectively, 92% sensitivity for the three parameters and specificity 67%, 47% and 73% respectively. Lymph node sizes had no relation to the perfusion parameter values. Conclusion CT perfusion is a functional imaging tool with promising results in differentiating between responders and non-responders in treated lymphoma patients, using BF, BV and PS.

Characterization of different Lactobacillus bacterial strains has an increasing demand due to the... more Characterization of different Lactobacillus bacterial strains has an increasing demand due to their potential health benefits. Classical cell counting methods are time-consuming and require well trained laboratory work, besides they are having higher subjectivity and error. The determination of electrical impedance spectrum is a promising method for the evaluation of changes in chemical structure and cell growth during the formation of yoghurt. Our aim is to use electrical impedance spectrum for the monitoring of cell growth of LABs. In this study different strains of Lactobacillus bulgaricus bacteria were used for the production of yoghurt from ultrahigh temperature processed (UHT) milk of 1.5% fat content. The cell count of the LABs was determined with plating method on MRS (De Man, Rogosa and Sharpe) agar in each hour during 12 h of cultivation at 37 °C. For impedance measurements, the real part (conductance) and the imaginary part (susceptance) of admittance (reciprocal value of...

Background: One of the frequent musculoskeletal problems is knee pain. The trend of increasing of... more Background: One of the frequent musculoskeletal problems is knee pain. The trend of increasing of knee pain among the populations is noted. Therefore, choosing a reliable screening tool with reasonable cost is mandatory. Although magnetic resonance imaging (MRI) is the gold standard imaging modality for knee soft tissue structures, it has been widely abused with its high cost. Ultrasound is an established modality to image the soft tissue structures of the knee. Objective: To show the role of ultrasound (US) in evaluating of knee pain and comparing the results with magnetic resonance imaging (MRI). Patients and methods: This study included 40 patients (18 males and 22 females) with an age ranging from 15-69 years. This study was carried out at the Radiology Department of Al-Azhar University Hospitals for ultrasonography and MRI examination during the period from October 2019 to November 2020. Results: Regarding meniscal horn tear, ultrasound detected tear in 2 cases, while MRI detec...

Innovation Strategies in the Food Industry

Sensors

Honey is produced by honeybees and is used as a food and medical product. Adulteration of honey h... more Honey is produced by honeybees and is used as a food and medical product. Adulteration of honey has been a problem for several years now because of the relatively high price of honey on the market according to its valuable composition. The aim of our study is to determine the physicochemical properties of authentic Hungarian linden and acacia honeys (pure samples or manipulated ones blended with sugar syrup) as well as commercially available blends of European Union (EU) non-European Union (non-EU) honeys. Authentic linden and acacia were blended with sugar syrup at 10%, 20% and 50% concentration levels, and physicochemical properties were determined according to the methods of the International Honey Commission. Our objectives also included testing of the performance of electronic sensory techniques (electronic tongue (ET) and electronic nose (EN)) in the detection of adulteration, and the results are compared to the sensory profile analysis. The results provide good average recogn...

Frontiers in Pharmacology

Ectonucleoside triphosphate diphosphohydrolases (NTPDases) catalyze the hydrolysis of nucleoside ... more Ectonucleoside triphosphate diphosphohydrolases (NTPDases) catalyze the hydrolysis of nucleoside tri- and di-phosphates to mono-phosphates. The products are subsequently hydrolyzed by ecto-5′-nucleotidase (ecto-5′-NT) to nucleosides. NTPDase inhibitors have potential as novel drugs, e.g., for the treatment of inflammation, neurodegenerative diseases, and cancer. In this context, a series of anthraquinone derivatives structurally related to the anthraquinone dye reactive blue-2 (RB-2) was synthesized and evaluated as inhibitors of human NTPDases utilizing a malachite green assay. We identified several potent and selective inhibitors of human NTPDase2 and -3. Among the most potent NTPDase2 inhibitors were 1-amino-4-(9-phenanthrylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate (20, PSB-16131, IC50 of 539 nM) and 1-amino-4-(3-chloro-4-phenylsulfanyl)phenylamino-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate (48, PSB-2020, IC50 of 551 nM). The most potent NTPDase3 inhibitors were 1-amino-4-[3-(4,6-dichlorotriazin-2-ylamino)-4-sulfophenylamino]-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate (42, PSB-1011, IC50 of 390 nM) and 1-amino-4-(3-carboxy-4-hydroxyphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate (33, PSB-2046, IC50 of 723 nM). The best NTPDase2 inhibitor 20 showed a non-competitive inhibition type, while the NTPDase3 inhibitor 42 behaved as a mixed-type inhibitor. These potent compounds were found to be selective vs. other NTPDases. They will be useful tools for studying the roles of NTPDase2 and -3 in physiology and under pathological conditions.

Bioorganic Chemistry

Inhibiting VEGFR-2 has been set up as a therapeutic strategy for treatment of cancer. Accordingly... more Inhibiting VEGFR-2 has been set up as a therapeutic strategy for treatment of cancer. Accordingly, new quinazoline-based derivatives having the structural features of VEGFR-2 inhibitors were designed and synthesized. Anti-proliferative activities were evaluated against three human cancer cell lines (HepG-2, MCF-7 and HCT-116) using MTT assay method. Doxorubicin and sorafenib were used as positive controls. Compounds 26b, 29a, 29b and 30 showed excellent anti-cancer activities against all cell lines. Moreover, compound 31 was the most active with IC 50 values of 3.97 ± 0.2, 4.83 ± 0.2 and 4.58 ± 0.3 µM, respectively. The most active cytotoxic agents were further evaluated in vitro for their VEGFR-2 inhibitory activities, compound 31 showed a high activity against VEGFR-2 with an IC50 value of 2.5 ± 0.04 µM, almost equal to that of sorafenib (IC50 = 2.4 ± 0.05 µM). Further studies revealed the ability of this promising quinazoline derivative 31 to induce apoptosis and arrest cell cycle growth at G2/M phase. In vivo antitumor activities of the synthesized compounds revealed that compounds 30 and 31 possessed significant tumor growth inhibition effect. Molecular docking studies were also performed and finally we can say that VEGFR-2 inhibition confers the reported cytotoxic activities.

The Journal of the World Universities Forum

Progress in Agricultural Engineering Sciences

People have recently started to pay more attention to the healthier lifestyle, which also include... more People have recently started to pay more attention to the healthier lifestyle, which also includes the consumption of more natural and less processed food products. Honey as one of the most often used natural sweeteners has also been reconsidered and more commonly used. However, honey has also been the target of food adulteration due to its emerging use and relatively high price. Therefore, there is an increasing need to develop rapid evaluation methods for the identification of honey from different sources. Experiments have been performed with 79 authentic honey samples of different floral and geographical origins, mainly from Hungary. The standard analytical parameters used to characterize the nutritional values of honey such as antioxidant capacity, polyphenol content, ash content, pH, conductivity have been determined. The samples were also analyzed with a benchtop near infrared (NIR) spectrometer to record their NIR spectra. The data acquired with NIR spectroscopy measurements ...

Progress in Agricultural Engineering Sciences

The study focused on the efficacy of ultrasonic method for identifying vegetable oils and their m... more The study focused on the efficacy of ultrasonic method for identifying vegetable oils and their mixtures in the formulation of frying oil and its ability in authentication of virgin olive oil. The ultrasonic propagation properties (velocity and Time of Flight (TOF)) were used to classify oil samples and their mixtures at 1 MHz. The results revealed the ability to classify oil types in terms of their level of un-saturation, besides it is to identify oil mixtures. Each oil sample could be grouped into different clusters using ultrasonic parameters. Hence, ultrasonic could be used to discriminate the vegetable oil types and their mixtures effectively as a rapid and continuous method in the industrial in-line quality control system of vegetable oils and their mixtures.

Honey has been widely used for health care and as sweetener since the ancient times. Due to its g... more Honey has been widely used for health care and as sweetener since the ancient times. Due to its great nutritional value and its high price, honey is one of the most adulterated products on the market. Therefore, there is a need to develop new quick measurement methods to recognize the adulteration. Almost 80 authentic honey samples of different floral and geographical origins were collected for our experiments, focusing mainly on Hungarian honey. Various analytical methods were used for the determination of the nutritional values of the honey samples, e.g. antioxidant capacity, polyphenol content, ash content, pH, conductivity, etc. These measurements aim to complete the scarcely available data on Hungarian honeys. In addition, we determined sensory properties by color and electronic tongue analyses. Electronic tongue enables easy sample preparation and results are delivered in a short time. Evaluating the results by different multivariate statistical methods, determination of the f...

The Egyptian Journal of Radiology and Nuclear Medicine, 2016

Abstract Objective Compare CT perfusion between responders and non-responders in patients with re... more Abstract Objective Compare CT perfusion between responders and non-responders in patients with residual cervical nodes after lymphoma treatment. Subjects and methods Twenty-seven patients with treated pathologically proved lymphoma were included in this prospective study, divided into two groups: responders (n. 15) and non-responders to treatment (n. 12). Blood flow (BF), blood volume (BV), mean transit time (MTT) and permeability (PS) were determined using CT perfusion. Lymph nodes sizes were measured (short axis). Results BF, BV and PS were significantly higher in non-responders group [93.3 (65.3–111) ml/min/100 g, 6.3 (5.2–8) ml/100 g and 18.9 (14.5–25.1) ml/min/100 g respectively] as compared to responders group [54 (43.8–77.5) ml/min/100 g, 4.1 (2.2–4.8) ml/100 g and 9.4 (7.5–14.5) ml/min/100 g respectively], [ p 0.019, 0.004 and 0.004 respectively]. MTT had a trend to be low in non-responders group, but did not reach statistical significance ( p 0.305). Cutoff value for BF, BV and PS was 59.5 ml/min/100 g, 3.6 ml/100 g and 11.1 ml/min/100 g respectively, 92% sensitivity for the three parameters and specificity 67%, 47% and 73% respectively. Lymph node sizes had no relation to the perfusion parameter values. Conclusion CT perfusion is a functional imaging tool with promising results in differentiating between responders and non-responders in treated lymphoma patients, using BF, BV and PS.