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Papers by mostafa ammori

Research paper thumbnail of Jongerenwerk en Homoseksualiteit

Research paper thumbnail of Antimicrobial efficacy of primary root canal filling material against Enterococcus faecalis

Enterococcus faecalis is associated with different root canal infections ,and it is the main caus... more Enterococcus faecalis is associated with different root canal infections ,and it is the main cause of chronic periapical lesions. Furthermore it is difficult to eliminate it from root canal in one visit or more ,so The aim of this study was to evaluate the antimicrobial activity of root canal paste on E. faecalis. 42 extracted single rooted primary teeth were manually prepared, sterilized, and then infected with clinical isolates of E. faecalis for 30 days. bacteria were exposed to ZOE and Ledermix pastes as intracanal medication for 1 week. Finally bacterial samples were collected, and colony-forming units were enumerated. none of pastes resulted in complete elimination of bacteria. The antimicrobial effect of ZOE was significantly better than Ledermix at P=.05. this study has shown that none of the sealers totally inhibited the growth of E. faecalis , and ZOE paste had the greater reduction of the CFU count for E. faecalis cells than Ledermix

Research paper thumbnail of The effect of chlorhexidine-thymol and fluoride varnishes on the levels of Streptococcus mutans in saliva in children aged 6–8 years

Background: Streptococcus mutans is considered as the main pathogenic factor for initiation and p... more Background: Streptococcus mutans is considered as the main pathogenic factor for initiation and progression of dental caries. Fluoride is one of the most effective agents used to control caries. Chlorhexidine (CHX) is the most antimicrobial agent against S. mutans and dental caries. Aims: The study aimed to compare the effectiveness of antimicrobial activity of CHX-thymol (CHX/T) and fluoride varnishes on S. mutans levels in children's saliva aged from 6 to 8 years old. Materials and Methods: The total number of children involved in this study is sixty, ages 6 and 8 years old. The participants were divided into three groups by block randomization: Group 1 CHX/T varnish, Group 2 fluoride varnish (f varnish, and Group 3 control group. Varnish was applied onto all tooth surfaces of the participants. At the baseline conditions, saliva samples were collected from the participants for bacterial examination test. This procedure was repeated in week 1, 4, and 12. Bacterial quantitative ...

Research paper thumbnail of Influence of different restorative materials on lysozyme and amylase activity of the salivary pellicle in situ

Journal of Biomedical Materials Research Part A, 2006

Lysozyme and amylase are the most abundant enzymatic components in the salivary pellicle. The pur... more Lysozyme and amylase are the most abundant enzymatic components in the salivary pellicle. The purpose of the present study was to determine the influence of different substrata on amylase and lysozyme activity in salivary pellicles formed in situ. Slabs (5 mm diameter) of bovine dentine and enamel, of titanium, gold alloy, resin composite, PMMA, amalgam, and feldspar ceramic were fixed on the buccal sites of individual splints worn by six subjects for 30 min to allow pellicle formation. Thereafter, slabs were removed from the trays and rinsed with running water. Lysozyme activity was determined via lysis of Micrococcus lysodeicticus. Amylase activity was measured with a photometric method using 2-chloro-4-nitrophenyl-4-O-␤-dgalactopyranosylmaltotriosid (GalG2CNP) as substrate. Both pellicle enzymes were evaluated in the immobilized as well as in the desorbed state. Salivary enzyme activities were also measured. All investigated pellicles exhibited lysozyme and amylase activity. Great intraindividual and in-terindividual differences were observed. Over all samples, immobilized amylase activity amounted to 0.65 Ϯ 0.64 mU/ cm 2. Immobilized lysozyme activity was 5.04 Ϯ 1.55 U/cm 2. There were no major effects of the substratum on pelliclebound amylase and lysozyme activity. Immobilized and desorbed enzyme activities revealed a strong correlation (lysozyme: r ϭ 0.700; amylase: r ϭ 0.990). Salivary enzyme activities had only little impact on pellicle-bound enzyme activities. Amylase and lysozyme are incorporated in the acquired in situ pellicle on different solid surfaces in an active conformation. Dental material and enzyme activity in the saliva have only little impact on enzymatic activity in the pellicle in situ.

Research paper thumbnail of Antimicrobial efficacy of primary root canal filling material against Enterococcus faecalis

Enterococcus faecalis is associated with different root canal infections ,and it is the main caus... more Enterococcus faecalis is associated with different root canal infections ,and it is the main cause of chronic periapical lesions. Furthermore it is difficult to eliminate it from root canal in one visit or more ,so The aim of this study was to evaluate the antimicrobial activity of root canal paste on E. faecalis. 42 extracted single rooted primary teeth were manually prepared, sterilized, and then infected with clinical isolates of E. faecalis for 30 days. bacteria were exposed to ZOE and Ledermix pastes as intracanal medication for 1 week. Finally bacterial samples were collected, and colony-forming units were enumerated. none of pastes resulted in complete elimination of bacteria. The antimicrobial effect of ZOE was significantly better than Ledermix at P=.05. this study has shown that none of the sealers totally inhibited the growth of E. faecalis , and ZOE paste had the greater reduction of the CFU count for E. faecalis cells than Ledermix

Research paper thumbnail of Jongerenwerk en Homoseksualiteit

Research paper thumbnail of Antimicrobial efficacy of primary root canal filling material against Enterococcus faecalis

Enterococcus faecalis is associated with different root canal infections ,and it is the main caus... more Enterococcus faecalis is associated with different root canal infections ,and it is the main cause of chronic periapical lesions. Furthermore it is difficult to eliminate it from root canal in one visit or more ,so The aim of this study was to evaluate the antimicrobial activity of root canal paste on E. faecalis. 42 extracted single rooted primary teeth were manually prepared, sterilized, and then infected with clinical isolates of E. faecalis for 30 days. bacteria were exposed to ZOE and Ledermix pastes as intracanal medication for 1 week. Finally bacterial samples were collected, and colony-forming units were enumerated. none of pastes resulted in complete elimination of bacteria. The antimicrobial effect of ZOE was significantly better than Ledermix at P=.05. this study has shown that none of the sealers totally inhibited the growth of E. faecalis , and ZOE paste had the greater reduction of the CFU count for E. faecalis cells than Ledermix

Research paper thumbnail of The effect of chlorhexidine-thymol and fluoride varnishes on the levels of Streptococcus mutans in saliva in children aged 6–8 years

Background: Streptococcus mutans is considered as the main pathogenic factor for initiation and p... more Background: Streptococcus mutans is considered as the main pathogenic factor for initiation and progression of dental caries. Fluoride is one of the most effective agents used to control caries. Chlorhexidine (CHX) is the most antimicrobial agent against S. mutans and dental caries. Aims: The study aimed to compare the effectiveness of antimicrobial activity of CHX-thymol (CHX/T) and fluoride varnishes on S. mutans levels in children's saliva aged from 6 to 8 years old. Materials and Methods: The total number of children involved in this study is sixty, ages 6 and 8 years old. The participants were divided into three groups by block randomization: Group 1 CHX/T varnish, Group 2 fluoride varnish (f varnish, and Group 3 control group. Varnish was applied onto all tooth surfaces of the participants. At the baseline conditions, saliva samples were collected from the participants for bacterial examination test. This procedure was repeated in week 1, 4, and 12. Bacterial quantitative ...

Research paper thumbnail of Influence of different restorative materials on lysozyme and amylase activity of the salivary pellicle in situ

Journal of Biomedical Materials Research Part A, 2006

Lysozyme and amylase are the most abundant enzymatic components in the salivary pellicle. The pur... more Lysozyme and amylase are the most abundant enzymatic components in the salivary pellicle. The purpose of the present study was to determine the influence of different substrata on amylase and lysozyme activity in salivary pellicles formed in situ. Slabs (5 mm diameter) of bovine dentine and enamel, of titanium, gold alloy, resin composite, PMMA, amalgam, and feldspar ceramic were fixed on the buccal sites of individual splints worn by six subjects for 30 min to allow pellicle formation. Thereafter, slabs were removed from the trays and rinsed with running water. Lysozyme activity was determined via lysis of Micrococcus lysodeicticus. Amylase activity was measured with a photometric method using 2-chloro-4-nitrophenyl-4-O-␤-dgalactopyranosylmaltotriosid (GalG2CNP) as substrate. Both pellicle enzymes were evaluated in the immobilized as well as in the desorbed state. Salivary enzyme activities were also measured. All investigated pellicles exhibited lysozyme and amylase activity. Great intraindividual and in-terindividual differences were observed. Over all samples, immobilized amylase activity amounted to 0.65 Ϯ 0.64 mU/ cm 2. Immobilized lysozyme activity was 5.04 Ϯ 1.55 U/cm 2. There were no major effects of the substratum on pelliclebound amylase and lysozyme activity. Immobilized and desorbed enzyme activities revealed a strong correlation (lysozyme: r ϭ 0.700; amylase: r ϭ 0.990). Salivary enzyme activities had only little impact on pellicle-bound enzyme activities. Amylase and lysozyme are incorporated in the acquired in situ pellicle on different solid surfaces in an active conformation. Dental material and enzyme activity in the saliva have only little impact on enzymatic activity in the pellicle in situ.

Research paper thumbnail of Antimicrobial efficacy of primary root canal filling material against Enterococcus faecalis

Enterococcus faecalis is associated with different root canal infections ,and it is the main caus... more Enterococcus faecalis is associated with different root canal infections ,and it is the main cause of chronic periapical lesions. Furthermore it is difficult to eliminate it from root canal in one visit or more ,so The aim of this study was to evaluate the antimicrobial activity of root canal paste on E. faecalis. 42 extracted single rooted primary teeth were manually prepared, sterilized, and then infected with clinical isolates of E. faecalis for 30 days. bacteria were exposed to ZOE and Ledermix pastes as intracanal medication for 1 week. Finally bacterial samples were collected, and colony-forming units were enumerated. none of pastes resulted in complete elimination of bacteria. The antimicrobial effect of ZOE was significantly better than Ledermix at P=.05. this study has shown that none of the sealers totally inhibited the growth of E. faecalis , and ZOE paste had the greater reduction of the CFU count for E. faecalis cells than Ledermix