manal elhalwagy - Academia.edu (original) (raw)
Papers by manal elhalwagy
International journal of health sciences
Oral administration of Acrylamide in our food produces adverse health effects through different v... more Oral administration of Acrylamide in our food produces adverse health effects through different vital systems. This study examined the effect of simultaneous oral supplementation of unicellular algae Chlorella vulgaris (CV) on thyroid gland disruption induced by intoxication with Acrylamide (ACR). Healthy male rats (n=60) distributed in six groups: -ve control, +ve control (CV 300 mg/kg), low dose Acrylamide (LACR, 5 mg/kg), high dose Acrylamide (HACR, 50 mg/kg), pre-administration of Chlorella Vulgaris with Acrylamide CV+ LACR (5mg/kg), and with Acrylamide CV+HACR (50 mg/kg). All groups received treatments orally for 30 days. ACR treated groups (LACR, HACR) demonstrated a significant elevation in oxidative stress biomarkers Malondialdehyde (MDA), 4-Hydroxynonenal (4-HNE), Nitric Oxide (NO) and pro-inflammatory cytokines C-reactive protein (CRP), Tumor Necrosis Factor alpha (TNF-α), Interleukin 6 (IL-6) and Interleukin 1 beta (IL-1β). In relation to antioxidant biomarkers Glutathi...
International Journal of Advanced Research in Biological Sciences (IJARBS), 2018
Aim: food additives are substances of natural or synthetic origin, which are added to foods to se... more Aim: food additives are substances of natural or synthetic origin, which are added to foods to serve a certain technological or sensory function. this work was aimed to investigate the effects of individual and combined treatment of artificial food additives on oxidative stress, antioxidant parameters in male juvenile albino rats. Exparimental Design:Thirty youngmale albino rats weighing around 90-110 gm. were divided into six groups (5 /cage); Group I: (Control untreated group), Group II: treated with MSG (300mg/kg), Group III: treated with Sodium benzoate (5mg /kg), Group IV: treat with Carmoisine (4mg/kg), Group V: treated with EDTA (2.5mg/kg), Group VI: treated with mixture of MSG (300/kg), Carmosine (4mg/kg), Sodium benzoate (5mg/kg) and EDTA (2.5 mg/ kg) simultaneously. All the treatments for 28 days. Results:The obtained results revealed elevation in oxidative stress markers malondialdehyde MDA and protein carbonyl PC in individually treated MSG and sodium benzoate and remarkable in mixture group. Significant reduction in catalase, total antioxidant capacity, and glutathione content were recorded in individually treated groups and pronounced in mixture group.Concolusion: food additives affect adversely on oxidative stress and antioxidant markers that may cause adverse health effects. It is advisable to limit the uses of commodities containing these additives.
International Journal of Advanced Research in Biological Sciences (IJARBS), 2019
Aim: Pesticide residues contamination in exported grapes has been monitored in this study, then t... more Aim: Pesticide residues contamination in exported grapes has been monitored in this study, then the obtained results of this survey were used to create laboratory simulations of mixtures that would be encountered by a consumption of contaminated grapes. Experiment: Malathion, lambada-cyhalothrin and diniconazole were the highest frequented pesticides in grapes. It has been detected in 45 (75%) samples out of 60 grapes samples were analyzed. The impact of those pesticides residues were applied in mixtures typical of what real exposure would experience has been studied. Rats have been treated with two different concentrations the higher and lower concentration which detected in grapes. Results: Our results reported that the exposure to pesticide mixture induced dose dependent effect in experimental animals expressed by significant inhibition of AChE, hyperglycemia that it is a mechanism of oxidative stress induced degeneration in liver and pancreas tissues. Conclusion: results indicated that low-level, widespread exposures to pesticide mixtures could impair biological activities in rats. It also shows that traditional approaches to assessing the pesticide toxicity effects one pesticide at a time completely miss understand the health impact of pesticides residues exposure. Further studies to examine the molecular changes of pesticides mixtures exposure are recommended.
![Research paper thumbnail of Corrigendum to "Microplastic in the gastrointestinal tract of fishes along the Saudi Arabian Red Sea coast" [Mar. Pollut. Bull. 131 (Part A) (2018) 1-808]](https://attachments.academia-assets.com/94877058/thumbnails/1.jpg)
](Marine pollution bulletin, Jan 29, 2018
Marine pollution bulletin, 2018
This study assesses the presence of microplastic litter in the contents of the gastrointestinal t... more This study assesses the presence of microplastic litter in the contents of the gastrointestinal tract of 26 commercial and non-commercial fish species from four difference habitats sampled along the Saudi Arabian coast of the Red Sea. A total of 178 individual were examined for microplastics. In total, 26 microplastic fragments were found. Of these, 16 being films (61.5%) and 10 being fishing thread (38.5%). FTIR analysis revealed that the most abundant polymers were polypropylene and polyethylene. The grouper (Epinephelus spp.) sampled at Jazan registered the highest number of ingested microplastics. This fish species is benthic and feeds on benthic invertebrates. Although differences in the abundance of microplastic ingestion among species were not statistically significant, a significant change was observed when the level of ingestion of microplastics particles was compared among the habitats. The higher abundance of microplastics particles may be related to the habitats of fish ...
Molecules (Basel, Switzerland), Jan 8, 2017
Central nervous system cytotoxicity is linked to neurodegenerative disorders. The objective of th... more Central nervous system cytotoxicity is linked to neurodegenerative disorders. The objective of the study was to investigate whether monosodium glutamate (MSG) neurotoxicity can be reversed by natural products, such as ginger or propolis, in male rats. Four different groups of Wistar rats were utilized in the study. Group A served as a normal control, whereas group B was orally administered with MSG (100 mg/kg body weight, via oral gavage). Two additional groups, C and D, were given MSG as group B along with oral dose (500 mg/kg body weight) of either ginger or propolis (600 mg/kg body weight) once a day for two months. At the end, the rats were sacrificed, and the brain tissue was excised and levels of neurotransmitters, ß-amyloid, and DNA oxidative marker 8-OHdG were estimated in the brain homogenates. Further, formalin-fixed and paraffin-embedded brain sections were used for histopathological evaluation. The results showed that MSG increased lipid peroxidation, nitric oxide, neuro...
Asian Journal of Chemistry, 2014
Diclofop-methyl is being increasingly used as an herbicide under intensive cultivation to control... more Diclofop-methyl is being increasingly used as an herbicide under intensive cultivation to control weeds in wheat. The bioconcentration of diclofop-methyl in two different groups of freshwater fish (Oreochromis niloticus) was investigated after 28 days of exposure. Low and high concentrations applied to two different fish groups were 1/10 (0.19 mg L-1) and 1/3 (0.63 mg L-1) of the determined 96 h-LC50, respectively. The bioconcentration factors for whole fish were 7.05 and 9.21 for fish exposed to lower and higher concentrations, respectively; while the respective bioconcentration factors for muscle tissue were 1.61 and 2.15 LC for fish exposed to lower and higher concentrations, respectively. Diclofop-acid was the main metabolite during the exposure period, ranging concentrations from 5 to 8% of the parent diclofop-methyl concentration.
International journal of clinical and experimental medicine, 2014
The herbicides diclofop-methyl and the fungicide difenoconazole are widely used in agriculture an... more The herbicides diclofop-methyl and the fungicide difenoconazole are widely used in agriculture and may lead to serious toxicity risks. However, limited studies have been done to evaluate differences in the metabolic effects of these herbicides. Difenoconazole (10 mg/kg) and Diclofop-methyl (1 mg/kg) were orally administrated individually (Groups 1 and 2 respectively) as well as combined (G3) to rats for 28 days. In all treated groups, alanine aminotransferase (ALT) and urea were significantly higher than the control group. Plasma creatinine was also significantly higher in groups G1 and G2 than control. Significant inhibition in gamma glutamyltransferase (γGT) was observed in all treated groups, in addition to significant inhibition of plasma acetylcholinesterase enzyme (AChE) in G3 (p < 0.01). There was no effect in aspartate aminotransferase (AST) and albumin. Total plasma triiodothy-ronine (T3) hormone was significantly higher in groups G2 and G3 (p < 0.01), but significant...
Annual Research & Review in Biology, 2014
The present work aimed to evaluate the effects potentiality ofeach of green tea (GT) polyphenols ... more The present work aimed to evaluate the effects potentiality ofeach of green tea (GT) polyphenols and garlic (Ga) aqueous extract and their combination oin reducingpesticide (durasin) induced somechanges in plasma levels of some indices of oxidative stressbiochemical changes as well asand plasma protein electrophoretic pattern changes induced due to intoxication with commercial formulation durasin (60% diazinon and 0.5%deltamethrin)in albino rats. The Albino rats(40) were divided into 8 groupsof five rats (5) each and treated orally for 14 days as follows: G1 (control),G 2 with (GT +ve): 200mg GT /animal, G3with 500mg3 (Ga +ve) 1ml of 50% of aqueous extract of garlic, G4 (GT + Ga): with 200mg green tea/animal and 1ml of 50% of aqueous extract500mg of garlic,G 5 with (D):20mg /Kgmbwt (1/14 LD50) of durasin,G6 (GT + D)with Green tea and Durasin, G7 (Ga + D)with Garlic and Durasin, G8 (GT + Ga + D)withGreen tea, Garlic and Durasin groups were treated orally for 14 days. Blood Plasma activities of acetylcholinesterase (AChE), glutathione-S-transferase(GST) and glutathione reductase (GR) activities and levels of malondialdehyde (MDA) and total reduced glutathione (GSH)samples were analyzed using standard methods. taken at the end of treatment period for further biochemical studies. Durasin intoxication induced significant inhibition in plasma acetylcholinesterase (AChE), elevation in malondialdehyde (MDA), reduction in total reduced glutathione (GSH), decrease plasma activities of glutathione-S-transferase(GST) and glutathione reductase activities (GR). It elevated plasma MDA, decreased plasma totalGSH change. In addition to the changes in plasma protein electrophoretic pattern. Individual supplementation with GT showinduced slight improvement in AchE, GSH and MDA. On the other hand, supplementation with aqueous extract of garlic induced pronounced significant (P < 0.05)enhancement in the level of GSH in the intoxicated animals.While However combined supplementation (with GT and Ga) indicates significant (P < 0.05)+Ga induced pronounced counteracting effect in MDA,GSH,GST and GR. The Meanwhile, they tried to counteract effect of extracts on changes in plasma protein pattern was not remarkable this may be due to plasma protein half life. but it might need along time of treatment for further improvement.The finding of this research work is indicating anti insecticides and/ or anti oxidative stress of green tea and garlic probably due their phytochemical contents.
Annual Research & Review in Biology, 2014
ABSTRACT Female Baladi goats were used to investigate the toxicological effects of 2,3,7,8- tetra... more ABSTRACT Female Baladi goats were used to investigate the toxicological effects of 2,3,7,8- tetrachlorodibenzo-p-dioxin (TCDD) on some serum hormones and blood features. Animals were divided into two groups, group 1: served as control, group 2: animals were orally administered with three repeated doses (0.23μg/Kg body weight) of TCDD with 2 days interval between dosing. Results revealed that exposure to TCDD induced reduction in serum estradiol, progesterone and prolactin levels and elevation in glucocorticoid hormone cortisol throughout the different studied periods (48h, 96h and 16 days commenced the last intoxicated dose). The adverse impact of TCDD on goat reproductive hormones was confirmed by histopatholgical observations on their uteri and ovaries after 16 days commenced the last intoxicated dose. Intoxication of goats with TCDD showed also marked decreases in hemoglobin (Hb) concentration, red blood cell count (RBC), packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH)and mean corpuscular hemoglobin concentrations (MCHC) compared with normal healthy group. Pronounced decreases in total white blood cells (WBC) count as well as in its differential percentages namely lymphocytes, monocytes and eosinophils were also noticed in intoxicated animals compared with normal ones, while the percentage of neutrophils showed significant increase. In conclusion, the results of this study indicated that oral exposure of female goats to repeated doses of TCDD caused endocrine disruption which may lead to adverse impact on their reproductive performance. Moreover, toxicity of TCDD induced immunosuppressive effect and anemia as indicated by its deleterious action on different hematological parameters.
Nature and Science, 2009
Page 1. , 0740-1545 ISSN , )5(7; 2009 ature and Science, N net. sciencepub . www :// http , com. ... more Page 1. , 0740-1545 ISSN , )5(7; 2009 ature and Science, N net. sciencepub . www :// http , com. naturesciencej@gmail 29 Which can Attenuate Hepatotoxicity Induced By Pesticides Mixture Natural or Synthetic Phenolic Antioxidant Azza Gawish a and Manal EA Elhalwagy b ...
Asian Journal of Chemistry, 2013
Recently, increases in the prevalence rates of chronic diseases along with rapid increases in agi... more Recently, increases in the prevalence rates of chronic diseases along with rapid increases in aging populations have led to great demands for foods with health-improving functionalities. A considerable body of literature supports the role of oxidative stress in the pathogenesis of age-related human diseases such as cancer, diabetes, immune system decline and brain dysfunction 1,2. There have been various types of phytonutrients with multiple biological effects including anti-inflammatory, anticancer, antiallergic, antiviral and antiaging activities 3-5 , therefore much attention has been focused on the biological properties of natural foods and herbs. Amaranthus plants (Amaranthaceae) are spread throughout the world, growing under a wide range of climatic conditions and they are known as grains and leafy edible vegetables 6. Traditionally, Amaranthus viridis L. (Amaranthaceae) has been used in the treatment of dysentery, enteritis, hemorrhoids and kidney diseases in China. A. viridis L. has been reported for its antiinflammatory, antioxidant, antihyperlipedemic and antidiabetic activities 7,8. At the same time, the young leaves of A. viridis are used as a vegetable. There are almost no relevant reports about the investigation of the bioactivities of Amaranthus viridis L. extracts. Thus, in this study, we evaluated the antioxidant, antiinflammatory and anticancer activities of ethyl ether and ethyl acetate extracts from Amaranthus viridis L. using in vitro assays.
African Journal of Biotechnology, 2012
Zizyphus nummularis Wt. & Arm. (Family: Rhamnaceceae) has ethnomedicinal value used by the local ... more Zizyphus nummularis Wt. & Arm. (Family: Rhamnaceceae) has ethnomedicinal value used by the local tribe of south Orissa, India for the treatment of typhoid, dysentery and diarrhea. The ethanol and chloroform extracts from various parts of the plant stem, leaves and root were analyzed for the presence of bioactive compounds. In addition, antimicrobial activity of the extracts from various parts where separately assessed against Salmonella typhimurium, Bacillus subtilis and Escherichia coli. Furthermore, minimum inhibitory concentration (MIC) of the extracts was evaluated. Bioactive compounds from all the parts were found to contain tannin, flavonoids, steroids, glycosides and alkaloids in addition to certain other minor compounds. Maximum zone of inhibition was found with the ethanolic root extract against S. typhimurium (20.2 mm) as compared to other microbes tested. The MIC of ethanolic root extracts was found to be 7.81 mgml-1 against S. typhimurium and E. coli whereas the same MIC of chloroform extracts was found to be effective against S. typhimurium. The study demonstrates that the root of Z. nummularies can affectively be used against microbial infection. However, a further study is needed to advocate the safety of the bioactive compounds in therapeutic use.
International journal of clinical and experimental medicine, 2015
Despite the known adverse effects of lambda cyhalothrin insecticide, little is known about its he... more Despite the known adverse effects of lambda cyhalothrin insecticide, little is known about its hepatopancreatic intoxication effects. The present study was carried out to elucidate sub-chronic effect of Karat 2.5% EC formulation of lambda cyhalothrin on male albino rats. To explore the effects of exposure to lambda cyhalothrin on rats and its mechanism, low (1/40 of LD50, 5 mg/kg/day) and high dose (1/4 of LD50, 50 mg/kg/day) lambda cyhalothrin were applied to rats via drinking water for 3 months. Blood samples were collected monthly, and the animals were dissected for liver and pancreas's examination at the end of the experiment. Lambda cyhalothrin administration was associated with the elevation in lipid peroxidation marker, malondialdehyde (MDA), reduction in SH-protein a major marker for antioxidant, as well as basel paraoxonase (PON) in both treated groups throughout the experimental periods. In addition, significant elevations in liver enzymes alanin amino transferase, (AL...
International journal of clinical and experimental medicine, 2015
This study aimed to investigate age dependent immune-system response versus exposure to different... more This study aimed to investigate age dependent immune-system response versus exposure to different doses of mixture of (chlorpyrifos, profenofose, and fenitrothion) and/or combined with 60 and 250 mg kg(-1) alpha lipoic acid and garlic, respectively. 120 males of albino rats were divided to two groups according to age; weaning group (2 months age and 60-80 gm.), adult (6 months and 180-200 gm). Each age was divided into 6 subgroups treated orally for 3 months , G1 (control), G2 high dose (HDPM) CPF10 mg kg(-1), PRO 3 mg kg(-1), FEN 6 mg kg(-1), G3 low dose (LDPM) CPF 1 mg kg(-1), PFN 0.3 mg kg(-1) and FEN 0.6 mg kg(-1), G4 AOX (alpha lipoic + Garlic), G5 HDPM + AOX and G6 LDPM + AOX. Results showed significant inhibition in serum acetylcholinesterase (AChE), elevation in malondialdehyde (MDA) concurrent with reduction in total reduced glutathione (GSH) in both ages was recorded as well as, decrease in IGG, IGM, Lymphocyte transformation and Phagocytosis humeral and cellular immunity ...
American Chemical Science Journal, 2015
The current investigation aimed to evaluate endocrine disruption and oxidative stress of subchron... more The current investigation aimed to evaluate endocrine disruption and oxidative stress of subchronic co-exposure of Malathion (500 ppm), Arsenic (50 ppm) and Paracetamol (100 mg/kg b.w) in white rats. Eighty adult male albino rats weighing 120-140 g were orally treated with these agents individually or in-combination for 28 days (4weeks).Changes in body weight gain, blood total triiodothyronin (T3), thyroxin (T4), testosterone, lipid peroxidation (MDA), total antioxidants capacity (TAC) and total proteins (TP); besides some histopathological and morphometrical analysis of testis and thyroid tissues were evaluated. Exposure to selected xenobiotics individually or combined showed general decrease in body weight; a significant reduction in T3 level in Paracetamol combined treated groups; a significant increase in T4 among Arsenic treated groups; as well as reduction in testosterone level in all treated groups. A significant elevation in MDA concomitant with reduction in TAC and signific...
Australian Journal of Basic and Applied Sciences
Current study was undertaken to evaluate the impact of repeated co-exposure of the widely used an... more Current study was undertaken to evaluate the impact of repeated co-exposure of the widely used analgesic-antipyretic drug Acetaminophen (Paracetamol), Malathion (organophosphates pesticides), and metalloid (Arsenic) on the functions of the liver and pancreas of male albino rats. Eighty male albino rats weighing 140-160g were orally treated with Acetaminophen (100 mg/kg b.w), Malathion (30 mg/kg b.w) or Arsenic (1.5 mg/kg b.w) individually and in-combination for 28 days. Biochemical results revealed that all treatments under investigation showed significant increase in serum aspartate aminotransferase (AST) alanine aminotransferase (ALT) and amylase activities, as well as fasting glucose levels. Lipase recorded fluctuation in its activity according to treatment group, whereas cholinesterase activity (ChE) showed non–significant changes except for malathion treatment. In contrast, paraoxonase (PON1) and insulin activities were significantly declined after single and combined treatment...
Journal of Animal and Veterinary Advances
Fenitrothion is being increasingly used as an insecticide under intensive cultivation to control ... more Fenitrothion is being increasingly used as an insecticide under intensive cultivation to control vegetables and fruits pests. The bioconcentration of fenitrothion two different groups of freshwater fish (Oreochromis niloticus) was investigated after 28 days of exposure. One tenth of the determined 96h-LC50 concentrations applied to fish. Fenitrothion concentration was 3.85 mg kg-1 and the bioconcentration factors (BCFs) after the experimental period 28 days 675.43 for whole fish body; while the concentration was 1.51 mg kg-1 with respective BCFs was reached 264.91 for muscle tissue were 1.61 after daily exposure during the experimental time 28 days. Results concluded that the fenitrothion has ability to fenitrothion bioconcentration factor was high in whole fish compared to muscle tissue.
International journal of health sciences
Oral administration of Acrylamide in our food produces adverse health effects through different v... more Oral administration of Acrylamide in our food produces adverse health effects through different vital systems. This study examined the effect of simultaneous oral supplementation of unicellular algae Chlorella vulgaris (CV) on thyroid gland disruption induced by intoxication with Acrylamide (ACR). Healthy male rats (n=60) distributed in six groups: -ve control, +ve control (CV 300 mg/kg), low dose Acrylamide (LACR, 5 mg/kg), high dose Acrylamide (HACR, 50 mg/kg), pre-administration of Chlorella Vulgaris with Acrylamide CV+ LACR (5mg/kg), and with Acrylamide CV+HACR (50 mg/kg). All groups received treatments orally for 30 days. ACR treated groups (LACR, HACR) demonstrated a significant elevation in oxidative stress biomarkers Malondialdehyde (MDA), 4-Hydroxynonenal (4-HNE), Nitric Oxide (NO) and pro-inflammatory cytokines C-reactive protein (CRP), Tumor Necrosis Factor alpha (TNF-α), Interleukin 6 (IL-6) and Interleukin 1 beta (IL-1β). In relation to antioxidant biomarkers Glutathi...
International Journal of Advanced Research in Biological Sciences (IJARBS), 2018
Aim: food additives are substances of natural or synthetic origin, which are added to foods to se... more Aim: food additives are substances of natural or synthetic origin, which are added to foods to serve a certain technological or sensory function. this work was aimed to investigate the effects of individual and combined treatment of artificial food additives on oxidative stress, antioxidant parameters in male juvenile albino rats. Exparimental Design:Thirty youngmale albino rats weighing around 90-110 gm. were divided into six groups (5 /cage); Group I: (Control untreated group), Group II: treated with MSG (300mg/kg), Group III: treated with Sodium benzoate (5mg /kg), Group IV: treat with Carmoisine (4mg/kg), Group V: treated with EDTA (2.5mg/kg), Group VI: treated with mixture of MSG (300/kg), Carmosine (4mg/kg), Sodium benzoate (5mg/kg) and EDTA (2.5 mg/ kg) simultaneously. All the treatments for 28 days. Results:The obtained results revealed elevation in oxidative stress markers malondialdehyde MDA and protein carbonyl PC in individually treated MSG and sodium benzoate and remarkable in mixture group. Significant reduction in catalase, total antioxidant capacity, and glutathione content were recorded in individually treated groups and pronounced in mixture group.Concolusion: food additives affect adversely on oxidative stress and antioxidant markers that may cause adverse health effects. It is advisable to limit the uses of commodities containing these additives.
International Journal of Advanced Research in Biological Sciences (IJARBS), 2019
Aim: Pesticide residues contamination in exported grapes has been monitored in this study, then t... more Aim: Pesticide residues contamination in exported grapes has been monitored in this study, then the obtained results of this survey were used to create laboratory simulations of mixtures that would be encountered by a consumption of contaminated grapes. Experiment: Malathion, lambada-cyhalothrin and diniconazole were the highest frequented pesticides in grapes. It has been detected in 45 (75%) samples out of 60 grapes samples were analyzed. The impact of those pesticides residues were applied in mixtures typical of what real exposure would experience has been studied. Rats have been treated with two different concentrations the higher and lower concentration which detected in grapes. Results: Our results reported that the exposure to pesticide mixture induced dose dependent effect in experimental animals expressed by significant inhibition of AChE, hyperglycemia that it is a mechanism of oxidative stress induced degeneration in liver and pancreas tissues. Conclusion: results indicated that low-level, widespread exposures to pesticide mixtures could impair biological activities in rats. It also shows that traditional approaches to assessing the pesticide toxicity effects one pesticide at a time completely miss understand the health impact of pesticides residues exposure. Further studies to examine the molecular changes of pesticides mixtures exposure are recommended.
Marine pollution bulletin, Jan 29, 2018
Marine pollution bulletin, 2018
This study assesses the presence of microplastic litter in the contents of the gastrointestinal t... more This study assesses the presence of microplastic litter in the contents of the gastrointestinal tract of 26 commercial and non-commercial fish species from four difference habitats sampled along the Saudi Arabian coast of the Red Sea. A total of 178 individual were examined for microplastics. In total, 26 microplastic fragments were found. Of these, 16 being films (61.5%) and 10 being fishing thread (38.5%). FTIR analysis revealed that the most abundant polymers were polypropylene and polyethylene. The grouper (Epinephelus spp.) sampled at Jazan registered the highest number of ingested microplastics. This fish species is benthic and feeds on benthic invertebrates. Although differences in the abundance of microplastic ingestion among species were not statistically significant, a significant change was observed when the level of ingestion of microplastics particles was compared among the habitats. The higher abundance of microplastics particles may be related to the habitats of fish ...
Molecules (Basel, Switzerland), Jan 8, 2017
Central nervous system cytotoxicity is linked to neurodegenerative disorders. The objective of th... more Central nervous system cytotoxicity is linked to neurodegenerative disorders. The objective of the study was to investigate whether monosodium glutamate (MSG) neurotoxicity can be reversed by natural products, such as ginger or propolis, in male rats. Four different groups of Wistar rats were utilized in the study. Group A served as a normal control, whereas group B was orally administered with MSG (100 mg/kg body weight, via oral gavage). Two additional groups, C and D, were given MSG as group B along with oral dose (500 mg/kg body weight) of either ginger or propolis (600 mg/kg body weight) once a day for two months. At the end, the rats were sacrificed, and the brain tissue was excised and levels of neurotransmitters, ß-amyloid, and DNA oxidative marker 8-OHdG were estimated in the brain homogenates. Further, formalin-fixed and paraffin-embedded brain sections were used for histopathological evaluation. The results showed that MSG increased lipid peroxidation, nitric oxide, neuro...
Asian Journal of Chemistry, 2014
Diclofop-methyl is being increasingly used as an herbicide under intensive cultivation to control... more Diclofop-methyl is being increasingly used as an herbicide under intensive cultivation to control weeds in wheat. The bioconcentration of diclofop-methyl in two different groups of freshwater fish (Oreochromis niloticus) was investigated after 28 days of exposure. Low and high concentrations applied to two different fish groups were 1/10 (0.19 mg L-1) and 1/3 (0.63 mg L-1) of the determined 96 h-LC50, respectively. The bioconcentration factors for whole fish were 7.05 and 9.21 for fish exposed to lower and higher concentrations, respectively; while the respective bioconcentration factors for muscle tissue were 1.61 and 2.15 LC for fish exposed to lower and higher concentrations, respectively. Diclofop-acid was the main metabolite during the exposure period, ranging concentrations from 5 to 8% of the parent diclofop-methyl concentration.
International journal of clinical and experimental medicine, 2014
The herbicides diclofop-methyl and the fungicide difenoconazole are widely used in agriculture an... more The herbicides diclofop-methyl and the fungicide difenoconazole are widely used in agriculture and may lead to serious toxicity risks. However, limited studies have been done to evaluate differences in the metabolic effects of these herbicides. Difenoconazole (10 mg/kg) and Diclofop-methyl (1 mg/kg) were orally administrated individually (Groups 1 and 2 respectively) as well as combined (G3) to rats for 28 days. In all treated groups, alanine aminotransferase (ALT) and urea were significantly higher than the control group. Plasma creatinine was also significantly higher in groups G1 and G2 than control. Significant inhibition in gamma glutamyltransferase (γGT) was observed in all treated groups, in addition to significant inhibition of plasma acetylcholinesterase enzyme (AChE) in G3 (p < 0.01). There was no effect in aspartate aminotransferase (AST) and albumin. Total plasma triiodothy-ronine (T3) hormone was significantly higher in groups G2 and G3 (p < 0.01), but significant...
Annual Research & Review in Biology, 2014
The present work aimed to evaluate the effects potentiality ofeach of green tea (GT) polyphenols ... more The present work aimed to evaluate the effects potentiality ofeach of green tea (GT) polyphenols and garlic (Ga) aqueous extract and their combination oin reducingpesticide (durasin) induced somechanges in plasma levels of some indices of oxidative stressbiochemical changes as well asand plasma protein electrophoretic pattern changes induced due to intoxication with commercial formulation durasin (60% diazinon and 0.5%deltamethrin)in albino rats. The Albino rats(40) were divided into 8 groupsof five rats (5) each and treated orally for 14 days as follows: G1 (control),G 2 with (GT +ve): 200mg GT /animal, G3with 500mg3 (Ga +ve) 1ml of 50% of aqueous extract of garlic, G4 (GT + Ga): with 200mg green tea/animal and 1ml of 50% of aqueous extract500mg of garlic,G 5 with (D):20mg /Kgmbwt (1/14 LD50) of durasin,G6 (GT + D)with Green tea and Durasin, G7 (Ga + D)with Garlic and Durasin, G8 (GT + Ga + D)withGreen tea, Garlic and Durasin groups were treated orally for 14 days. Blood Plasma activities of acetylcholinesterase (AChE), glutathione-S-transferase(GST) and glutathione reductase (GR) activities and levels of malondialdehyde (MDA) and total reduced glutathione (GSH)samples were analyzed using standard methods. taken at the end of treatment period for further biochemical studies. Durasin intoxication induced significant inhibition in plasma acetylcholinesterase (AChE), elevation in malondialdehyde (MDA), reduction in total reduced glutathione (GSH), decrease plasma activities of glutathione-S-transferase(GST) and glutathione reductase activities (GR). It elevated plasma MDA, decreased plasma totalGSH change. In addition to the changes in plasma protein electrophoretic pattern. Individual supplementation with GT showinduced slight improvement in AchE, GSH and MDA. On the other hand, supplementation with aqueous extract of garlic induced pronounced significant (P < 0.05)enhancement in the level of GSH in the intoxicated animals.While However combined supplementation (with GT and Ga) indicates significant (P < 0.05)+Ga induced pronounced counteracting effect in MDA,GSH,GST and GR. The Meanwhile, they tried to counteract effect of extracts on changes in plasma protein pattern was not remarkable this may be due to plasma protein half life. but it might need along time of treatment for further improvement.The finding of this research work is indicating anti insecticides and/ or anti oxidative stress of green tea and garlic probably due their phytochemical contents.
Annual Research & Review in Biology, 2014
ABSTRACT Female Baladi goats were used to investigate the toxicological effects of 2,3,7,8- tetra... more ABSTRACT Female Baladi goats were used to investigate the toxicological effects of 2,3,7,8- tetrachlorodibenzo-p-dioxin (TCDD) on some serum hormones and blood features. Animals were divided into two groups, group 1: served as control, group 2: animals were orally administered with three repeated doses (0.23μg/Kg body weight) of TCDD with 2 days interval between dosing. Results revealed that exposure to TCDD induced reduction in serum estradiol, progesterone and prolactin levels and elevation in glucocorticoid hormone cortisol throughout the different studied periods (48h, 96h and 16 days commenced the last intoxicated dose). The adverse impact of TCDD on goat reproductive hormones was confirmed by histopatholgical observations on their uteri and ovaries after 16 days commenced the last intoxicated dose. Intoxication of goats with TCDD showed also marked decreases in hemoglobin (Hb) concentration, red blood cell count (RBC), packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH)and mean corpuscular hemoglobin concentrations (MCHC) compared with normal healthy group. Pronounced decreases in total white blood cells (WBC) count as well as in its differential percentages namely lymphocytes, monocytes and eosinophils were also noticed in intoxicated animals compared with normal ones, while the percentage of neutrophils showed significant increase. In conclusion, the results of this study indicated that oral exposure of female goats to repeated doses of TCDD caused endocrine disruption which may lead to adverse impact on their reproductive performance. Moreover, toxicity of TCDD induced immunosuppressive effect and anemia as indicated by its deleterious action on different hematological parameters.
Nature and Science, 2009
Page 1. , 0740-1545 ISSN , )5(7; 2009 ature and Science, N net. sciencepub . www :// http , com. ... more Page 1. , 0740-1545 ISSN , )5(7; 2009 ature and Science, N net. sciencepub . www :// http , com. naturesciencej@gmail 29 Which can Attenuate Hepatotoxicity Induced By Pesticides Mixture Natural or Synthetic Phenolic Antioxidant Azza Gawish a and Manal EA Elhalwagy b ...
Asian Journal of Chemistry, 2013
Recently, increases in the prevalence rates of chronic diseases along with rapid increases in agi... more Recently, increases in the prevalence rates of chronic diseases along with rapid increases in aging populations have led to great demands for foods with health-improving functionalities. A considerable body of literature supports the role of oxidative stress in the pathogenesis of age-related human diseases such as cancer, diabetes, immune system decline and brain dysfunction 1,2. There have been various types of phytonutrients with multiple biological effects including anti-inflammatory, anticancer, antiallergic, antiviral and antiaging activities 3-5 , therefore much attention has been focused on the biological properties of natural foods and herbs. Amaranthus plants (Amaranthaceae) are spread throughout the world, growing under a wide range of climatic conditions and they are known as grains and leafy edible vegetables 6. Traditionally, Amaranthus viridis L. (Amaranthaceae) has been used in the treatment of dysentery, enteritis, hemorrhoids and kidney diseases in China. A. viridis L. has been reported for its antiinflammatory, antioxidant, antihyperlipedemic and antidiabetic activities 7,8. At the same time, the young leaves of A. viridis are used as a vegetable. There are almost no relevant reports about the investigation of the bioactivities of Amaranthus viridis L. extracts. Thus, in this study, we evaluated the antioxidant, antiinflammatory and anticancer activities of ethyl ether and ethyl acetate extracts from Amaranthus viridis L. using in vitro assays.
African Journal of Biotechnology, 2012
Zizyphus nummularis Wt. & Arm. (Family: Rhamnaceceae) has ethnomedicinal value used by the local ... more Zizyphus nummularis Wt. & Arm. (Family: Rhamnaceceae) has ethnomedicinal value used by the local tribe of south Orissa, India for the treatment of typhoid, dysentery and diarrhea. The ethanol and chloroform extracts from various parts of the plant stem, leaves and root were analyzed for the presence of bioactive compounds. In addition, antimicrobial activity of the extracts from various parts where separately assessed against Salmonella typhimurium, Bacillus subtilis and Escherichia coli. Furthermore, minimum inhibitory concentration (MIC) of the extracts was evaluated. Bioactive compounds from all the parts were found to contain tannin, flavonoids, steroids, glycosides and alkaloids in addition to certain other minor compounds. Maximum zone of inhibition was found with the ethanolic root extract against S. typhimurium (20.2 mm) as compared to other microbes tested. The MIC of ethanolic root extracts was found to be 7.81 mgml-1 against S. typhimurium and E. coli whereas the same MIC of chloroform extracts was found to be effective against S. typhimurium. The study demonstrates that the root of Z. nummularies can affectively be used against microbial infection. However, a further study is needed to advocate the safety of the bioactive compounds in therapeutic use.
International journal of clinical and experimental medicine, 2015
Despite the known adverse effects of lambda cyhalothrin insecticide, little is known about its he... more Despite the known adverse effects of lambda cyhalothrin insecticide, little is known about its hepatopancreatic intoxication effects. The present study was carried out to elucidate sub-chronic effect of Karat 2.5% EC formulation of lambda cyhalothrin on male albino rats. To explore the effects of exposure to lambda cyhalothrin on rats and its mechanism, low (1/40 of LD50, 5 mg/kg/day) and high dose (1/4 of LD50, 50 mg/kg/day) lambda cyhalothrin were applied to rats via drinking water for 3 months. Blood samples were collected monthly, and the animals were dissected for liver and pancreas's examination at the end of the experiment. Lambda cyhalothrin administration was associated with the elevation in lipid peroxidation marker, malondialdehyde (MDA), reduction in SH-protein a major marker for antioxidant, as well as basel paraoxonase (PON) in both treated groups throughout the experimental periods. In addition, significant elevations in liver enzymes alanin amino transferase, (AL...
International journal of clinical and experimental medicine, 2015
This study aimed to investigate age dependent immune-system response versus exposure to different... more This study aimed to investigate age dependent immune-system response versus exposure to different doses of mixture of (chlorpyrifos, profenofose, and fenitrothion) and/or combined with 60 and 250 mg kg(-1) alpha lipoic acid and garlic, respectively. 120 males of albino rats were divided to two groups according to age; weaning group (2 months age and 60-80 gm.), adult (6 months and 180-200 gm). Each age was divided into 6 subgroups treated orally for 3 months , G1 (control), G2 high dose (HDPM) CPF10 mg kg(-1), PRO 3 mg kg(-1), FEN 6 mg kg(-1), G3 low dose (LDPM) CPF 1 mg kg(-1), PFN 0.3 mg kg(-1) and FEN 0.6 mg kg(-1), G4 AOX (alpha lipoic + Garlic), G5 HDPM + AOX and G6 LDPM + AOX. Results showed significant inhibition in serum acetylcholinesterase (AChE), elevation in malondialdehyde (MDA) concurrent with reduction in total reduced glutathione (GSH) in both ages was recorded as well as, decrease in IGG, IGM, Lymphocyte transformation and Phagocytosis humeral and cellular immunity ...
American Chemical Science Journal, 2015
The current investigation aimed to evaluate endocrine disruption and oxidative stress of subchron... more The current investigation aimed to evaluate endocrine disruption and oxidative stress of subchronic co-exposure of Malathion (500 ppm), Arsenic (50 ppm) and Paracetamol (100 mg/kg b.w) in white rats. Eighty adult male albino rats weighing 120-140 g were orally treated with these agents individually or in-combination for 28 days (4weeks).Changes in body weight gain, blood total triiodothyronin (T3), thyroxin (T4), testosterone, lipid peroxidation (MDA), total antioxidants capacity (TAC) and total proteins (TP); besides some histopathological and morphometrical analysis of testis and thyroid tissues were evaluated. Exposure to selected xenobiotics individually or combined showed general decrease in body weight; a significant reduction in T3 level in Paracetamol combined treated groups; a significant increase in T4 among Arsenic treated groups; as well as reduction in testosterone level in all treated groups. A significant elevation in MDA concomitant with reduction in TAC and signific...
Australian Journal of Basic and Applied Sciences
Current study was undertaken to evaluate the impact of repeated co-exposure of the widely used an... more Current study was undertaken to evaluate the impact of repeated co-exposure of the widely used analgesic-antipyretic drug Acetaminophen (Paracetamol), Malathion (organophosphates pesticides), and metalloid (Arsenic) on the functions of the liver and pancreas of male albino rats. Eighty male albino rats weighing 140-160g were orally treated with Acetaminophen (100 mg/kg b.w), Malathion (30 mg/kg b.w) or Arsenic (1.5 mg/kg b.w) individually and in-combination for 28 days. Biochemical results revealed that all treatments under investigation showed significant increase in serum aspartate aminotransferase (AST) alanine aminotransferase (ALT) and amylase activities, as well as fasting glucose levels. Lipase recorded fluctuation in its activity according to treatment group, whereas cholinesterase activity (ChE) showed non–significant changes except for malathion treatment. In contrast, paraoxonase (PON1) and insulin activities were significantly declined after single and combined treatment...
Journal of Animal and Veterinary Advances
Fenitrothion is being increasingly used as an insecticide under intensive cultivation to control ... more Fenitrothion is being increasingly used as an insecticide under intensive cultivation to control vegetables and fruits pests. The bioconcentration of fenitrothion two different groups of freshwater fish (Oreochromis niloticus) was investigated after 28 days of exposure. One tenth of the determined 96h-LC50 concentrations applied to fish. Fenitrothion concentration was 3.85 mg kg-1 and the bioconcentration factors (BCFs) after the experimental period 28 days 675.43 for whole fish body; while the concentration was 1.51 mg kg-1 with respective BCFs was reached 264.91 for muscle tissue were 1.61 after daily exposure during the experimental time 28 days. Results concluded that the fenitrothion has ability to fenitrothion bioconcentration factor was high in whole fish compared to muscle tissue.