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Papers by marshy belle

This study aimed to evaluate the in vitro thrombolytic potential of Mimosa pudica (Makahiya) usin... more This study aimed to evaluate the in vitro thrombolytic potential of Mimosa pudica (Makahiya) using the crude extract of the plant. This study utilized randomized controlled trial to determine the thrombolytic potential of Mimosa pudica (Makahiya) plant extract on the Citrated Platelet Poor Plasma. Makahiya plant was subjected to the phytochemical screening tests to determine the presence of glycosides. The study utilized twenty-one (21) healthy human subjects with normal Prothrombin time results to ensure that no recent or history of oral or intravenous anticoagulation therapy was taken. The volunteers’ blood sample were collected and subjected to centrifugation to yield citrated platelet-poor plasma. This study utilized the Euglobulin Clot Lysis Time to determine the thrombolytic potential of Mimosa pudica (Makahiya). 0.5 mL of citrated platelet poor plasma was placed into 3 test tubes labeled as positive control, experimental control, and negative control. The plasma was diluted and acidified to precipitate the euglobulin fraction. The tubes were added with 25 μL of thrombin to induce clot formation and the stopwatch was started immediately. Once clot was formed, test tube 1 was added with the 25 μL of Makahiya plant extract, and served as the experimental control. Test tube 2 was added with 25 μL of Streptokinase which served as the positive control. Test tube 3 only contained the citrated platelet poor plasma and served as the negative control.
The mean percent euglobulin clot lysis time in minutes of blood samples treated with Makahiya plant extract, Streptokinase, and untreated blood samples are 3.27 + .15, 2.33 + 3.7, and 360 respectively. Wilcoxon signed ranks test revealed that there was a significant difference between the median euglobulin clot lysis time in minutes of the blood samples treated with Makahiya extract and Streptokinase at .05 level of significance, z = -2.371 and p < .05. On the average, blood samples treated with makahiya extract (Median = 3.18) had longer euglobulin clot lysis time compared to the blood samples treated with streptokinase (Median = 2.54).
Based on the findings, Mimosa pudica (Makahiya) plant crude extract had an in vitro thrombolytic potential on citrated platelet poor plasma.

Background: Antibiotics and antimicrobials have been regarded as the universal cure for all treat... more Background: Antibiotics and antimicrobials have been regarded as the universal cure for all treatments because of their effectiveness against infectious diseases (Sannathimmappa et al., 2021). However, its irrational and inappropriate use led to the emergence of antimicrobial resistance (AMR) and is becoming a global health concern (Sannathimmappa et al., 2021; Lubwama et al., 2021).The awareness of medical students on the correct usage and practices of antibiotics and disease control practices are vital in forestalling the rising problem of AMR. With the lack of local studies , the researchers decided to conduct this study.
Objective: This study aimed to make a cross-sectional analysis of the knowledge, attitude, and behavior toward antibiotic use and antimicrobial resistance control strategies on the demographic characteristics of the medical students of CDU for the academic year 2021-2022.
Research Design: A descriptive cross-sectional survey design was employed.
Research Locale: The locale of this study was the College of Medicine of Cebu Doctors’ University. The university is a private educational institution catering mainly to fields involved in health services located on #1 Dr. P.V Larrazabal Jr. Avenue, North Reclamation Area, Mandaue City, Cebu, Philippines.
Research Respondents: The respondents were selected from Levels 1, 2, 3, and 4 of Cebu Doctors’ University - College of Medicine students enrolled during the Academic Year of 2021-2022. Quota sampling was used and the study garnered 233 respondents in which 39 students were from Level I, 71 from Level II, 67 from Level III and 56 from Level IV.
Research Instrument: The researchers utilized the questionnaire developed by Sannathimmappa et al. (2021) in their study. With Sannathimmappa’s approval, this questionnaire was modified by the researchers wherein 17 questions for knowledge, 5 for attitude, and 10 for behavior were added. This modified questionnaire was validated by three (3) experts. Reliability of the questionnaire are as follows: Knowledge on antibiotic use and antimicrobial resistance control strategies was evaluated with Kuder-Richardson 20 yielding a score of 0.631 which indicated low reliability; Attitude on antibiotic use and antimicrobial resistance control strategies was evaluated with Cronbach’s Alpha yielding a score of 0.712 which indicated acceptable reliability; Behavior on antibiotic use and antimicrobial resistance control strategies was also evaluated with Cronbach’s Alpha yielding a score of 0.818 which indicated good reliability.
Results: Results showed that the majority of the respondents are young adults (163, 70.0%), more than half were females (158, 67.8%), and were from the second year (71, 30.5%).
A positive attitude towards antibiotic use and antimicrobial resistance control strategies was shown by the majority of the respondents (231, 99.1%) and the minority had a negative attitude (2, 0.9%). A majority of the respondents have good behavior towards antibiotic use and antimicrobial resistance control strategies (223, 95.7%) while the minority had poor behavior (10, 4.3%). Majority of both young adults (129, 79.1%) and adults (55, 78.6%) regardless of sex and year level showed a good level of knowledge towards antibiotic use and antimicrobial resistance control strategies. For Attitude, both young adults (162, 99.4%) and adults (69, 98.6%) regardless of sex and year level had a positive attitude towards antibiotic use and antimicrobial resistance control strategies. Both young adults (153, 93.9%) and all adults (70, 100%) regardless of sex and year level also showed a positive behavior towards antibiotic use and antimicrobial
resistance control strategies.
Conclusion: Based from the gathered data, the Medical students had a good level of knowledge, a positive attitude, and a good behavior towards Antibiotic Use and Antimicobial Resistance Control Strategies across all year levels, age-groups, and sex.
Keywords: Knowledge, attitude, behavior, antibiotic use, antimicrobial resistance control strategies, medical students, demographic characteristics, cross-sectional analysis.