maryam montaseri - Academia.edu (original) (raw)
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Papers by maryam montaseri
International Archives of Occupational and Environmental Health
Journal of Advanced Biomedical Sciences
Background & Objectives: Staphylococcus spp. is a resident flora of the skin and mucosa of humans... more Background & Objectives: Staphylococcus spp. is a resident flora of the skin and mucosa of humans that can colonize the anterior nares of individuals. This cross-sectional study was conducted to determine the rate and antibiotic resistance pattern of nasal Staphylococcus aureus (S. aureus) carriers among the staff of Fasa hospital, southern Iran. Materials & Methods: Nasal swab samples were collected from 117 hospital staff working in 12 wards. Microbiological culture method was applied for S. aureus identification. The isolates were confirmed by tuf gene identification using PCR assay. Five isolates were randomly sequenced and phylogenetically analysed using MEGA software. The antimicrobial resistance pattern of the isolates was evaluated using the disc diffusion assay and the amplification of the methicillin resistance (mecA) gene. Results: The prevalence of S. aureus nasal carriers included 10.26% (n=12). The nasal carriers were identified in the wards of surgery ICU, gynecologi...
In this study cytotoxic effects of silver-chitosan nanocomposites with aqueous sodium-hydroxide s... more In this study cytotoxic effects of silver-chitosan nanocomposites with aqueous sodium-hydroxide solution (SCNC-ASHS), and aqueous acetic acid solution (SCNC-AAAS) were evaluated, in vitro. The morphology of the synthesized nanoparticles were characterized by Fourier-Transform Infrared Spectroscopy (FTIR), and Scanning Electron Microscopy (SEM). Their cytotoxicity were then evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) in concentrations of 1.56 to 400 µg/ml, and acridine orange/ethidium bromide (AO/EB) staining after 24h and 48h. Results showed the cytotoxicity of 400 µg/ml of SCNC-ASHS on Vero and HT-29 cells of 80.57% and 84.37% after 24h, and 82.20% and 84.84% after 48h. While, the values for SCNC-AAAS on Vero and HT-29 cell-lines were respectively 80.63% and 87.64% after 24h, and 83.60% and 87.44% after 48h. The most toxicity on HT-29 cells was belonged to SCNC-AAAS with IC50 of 40.4 µg/ml. In the staining procedure, cell viability for 25 ...
International Archives of Occupational and Environmental Health
Journal of Advanced Biomedical Sciences
Background & Objectives: Staphylococcus spp. is a resident flora of the skin and mucosa of humans... more Background & Objectives: Staphylococcus spp. is a resident flora of the skin and mucosa of humans that can colonize the anterior nares of individuals. This cross-sectional study was conducted to determine the rate and antibiotic resistance pattern of nasal Staphylococcus aureus (S. aureus) carriers among the staff of Fasa hospital, southern Iran. Materials & Methods: Nasal swab samples were collected from 117 hospital staff working in 12 wards. Microbiological culture method was applied for S. aureus identification. The isolates were confirmed by tuf gene identification using PCR assay. Five isolates were randomly sequenced and phylogenetically analysed using MEGA software. The antimicrobial resistance pattern of the isolates was evaluated using the disc diffusion assay and the amplification of the methicillin resistance (mecA) gene. Results: The prevalence of S. aureus nasal carriers included 10.26% (n=12). The nasal carriers were identified in the wards of surgery ICU, gynecologi...
In this study cytotoxic effects of silver-chitosan nanocomposites with aqueous sodium-hydroxide s... more In this study cytotoxic effects of silver-chitosan nanocomposites with aqueous sodium-hydroxide solution (SCNC-ASHS), and aqueous acetic acid solution (SCNC-AAAS) were evaluated, in vitro. The morphology of the synthesized nanoparticles were characterized by Fourier-Transform Infrared Spectroscopy (FTIR), and Scanning Electron Microscopy (SEM). Their cytotoxicity were then evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) in concentrations of 1.56 to 400 µg/ml, and acridine orange/ethidium bromide (AO/EB) staining after 24h and 48h. Results showed the cytotoxicity of 400 µg/ml of SCNC-ASHS on Vero and HT-29 cells of 80.57% and 84.37% after 24h, and 82.20% and 84.84% after 48h. While, the values for SCNC-AAAS on Vero and HT-29 cell-lines were respectively 80.63% and 87.64% after 24h, and 83.60% and 87.44% after 48h. The most toxicity on HT-29 cells was belonged to SCNC-AAAS with IC50 of 40.4 µg/ml. In the staining procedure, cell viability for 25 ...