maryam riaz - Academia.edu (original) (raw)

Papers by maryam riaz

Pakistan Journal of Agricultural Research, 2019

Diabetologia Praktyczna, 2020

Wstęp. Diabetes score to 10-punktowy, jednostronicowy kwestionariusz do omawiania zmiany stylu ży... more Wstęp. Diabetes score to 10-punktowy, jednostronicowy kwestionariusz do omawiania zmiany stylu życia, zaprojektowany do użytku w praktyce klinicznej. celem autorów była ocena kwestionariusza Diabetes Score pod kątem jego trafności i akceptowalności wśród osób z cukrzycą typu 2. Materiał i metody. Badanie obserwacyjne przeprowadzono z wykorzystaniem kwestionariuszy ankie-towanych u dorosłych pacjentów z cukrzycą typu 2 w trzech poradniach ambulatoryjnych. Wykorzystano kwestionariusz Diabetes Score do pomiaru przestrzegania zaleceń dotyczących diety, ćwiczeń i innych aspektów stylu życia. kwestionariusz daje intuicyjny wynik w zakresie od 0 do 100 punktów po dodaniu wszystkich 10 pozycji, które zostały ocenione przez pacjenta na 0, 5 lub 10 punktów. Wynik powyżej 60 punktów uznano za zadowalający. Wyniki. W badaniu wzięło udział łącznie 311 pacjentów, 56% kobiet, o medianie wieku 55 lat (zakres: 23 do 87). Ocena w skali Diabetes Score korelowała z kontrolą glikemii, HbA1c (r = –0,20) i stężeniem glukozy we krwi (r = –0,25; p < 0,001), co wskazuje na trafność kwestionariusza. Rzetelność wykazano na podstawie wewnętrznej spójności (alfa 0,577) i dyskryminacyjnej analizy czynnikowej. Jak pokazało modelowanie wieloczynnikowe, zwiększenie liczby punktów o 30 odpowiadało zmniejszeniu HbA1c o 1,0% (11 mmol/mol). Wnioski. kwestionariusz Diabetes Score to rzetelne i trafne narzędzie umożliwiające modyfikację stylu życia oraz zachowań chorych na cukrzycę. Ten krótki i darmowy kwestionariusz może być używany w poradniach diabetologicznych w celu omówienia zmiany stylu życia. Może służyć jako interwencja pierwszej linii u nowo zdiagnozowanych pacjentów z cukrzycą, po-zwalając na obniżenie kosztów opieki diabetologicznej

Objective; To determine frequency of BCG Vaccination in patients of tuberculous meningitis presen... more Objective; To determine frequency of BCG Vaccination in patients of tuberculous meningitis presenting at a tertiary care hospital. Study Design: Descriptive study. Place and Duration of Study: This study was conducted at the Department of Pediatric Medicine, Services Hospital Lahore from June 2016 to June 2017. Materials and Methods; A total of 167 Patients were included in the study. Patients with Tuberculous meningitis (TBM) less than 5 years of age were included. Patients having acute onset of encephalopathy and those with lumbar puncture contraindicated were excluded from our study. Demographic bio data (patient name, father name, age, gender, date of admission) was taken. After that complete history regarding presenting complaints and vaccination were taken. Physical examination will be done. BCG scar ark was identified on examination. CSF examination, Chest X-ray, CT scan brain ...

Objective; To determine the frequency of encephalopathy in children with enteric fever presenting... more Objective; To determine the frequency of encephalopathy in children with enteric fever presenting at a tertiary care hospital. Patients and methods; This descriptive, cross-sectional study was conducted at Department of Pediatric Medicine Services Hospital Lahore from January 2015 to December 2016. A total of 100 patients with enteric fever were recruited in our study. Results; Of these 100 study cases, 59 (59%) were boys and 41 (41%) were girls. Mean age of our study cases was 7.99 ± 3.28 years. Majority of the study cases with enteric fever i.e. 56 (56%) were age more than 7 years. Mean Temperature was 102.30 ± 0.45 F0 . Mean disease duration (enteric fever) was 5.47 ± 1.18 days (with minimum disease duration was 4 days while maximum duration of fever was 8 days). Of these 100 study cases, 51 (51%) belonged to the rural areas and 49 (49%) were from urban territories. While majority of these children belonged to the families having poor socio-economic status 67 (67%), middle income...

International Review of Management and Business Research, 2016

IntroductionOrganizations play an important role in the development of a country. The main purpos... more IntroductionOrganizations play an important role in the development of a country. The main purpose of a company is to earn good will and profit in a high level and for this purpose the organizations make sure that the employees are satisfied with their jobs or not. Employee job satisfaction is necessary for an organization.Job satisfaction is a very important part for an organization. In the organization many people are work for the sake of money and profit. If the workers of an organization are not satisfied with their work and feel stress on their jobs then the employees of the organization were not complete their work in a good way then this is negatively impact on the good will of the organization.Job stress can be described as the damaging physical and emotion of a person that arise when the wishes of the job do not compete with the abilities, means, or wants of the employee. Job stress can lead to bad strength and even damage. Mansoor, Fida, Nasir, & Ahmad (2011) says in that ...

In this study, the association between big five personality factors and level of forgiveness has ... more In this study, the association between big five personality factors and level of forgiveness has been measured with the help of two standardized questionnaires. These are Universiti Sains Malaysia Personality Inventory (USMaPand the Hartland Forgiveness Scale. A sample of 500 college students (200 males and 300 females; age range= 15-22 years) were selected by proportionate stratified sampling technique, from five major colleges of situated in Gujrat. The results showed significant correlation among big five personality factors and level of forgiveness. Results revealed that there was a significant and positive relationship among conscientiousness, extroversion, openness, agreeableness and level of forgiveness. It was concluded that neuroticism was significantly negatively correlated with level of forgiveness. The implication and the limitations of the study are discussed.

Clinical Diabetology, 2020

Background. Designed for use in clinical settings, the Diabetes Score is a 10-item, one-page ques... more Background. Designed for use in clinical settings, the Diabetes Score is a 10-item, one-page questionnaire for discussing lifestyle change. We aimed to evaluate the Diabetes Score questionnaire for its validity and acceptability among individuals with type 2 diabetes. Methods. An observational study was conducted using interviewer-administered questionnaires to adult patients with type 2 diabetes at three ambulatory clinics. We used the Diabetes Score questionnaire for measuring adherence to diet, exercise and other lifestyle recommendations. The questionnaire yields an intuitive score ranging from 0 to 100, by addition of each of the 10 items which are rated as 0, 5 or 10 by the patient. A score of more than 60 was considered satisfactory. Results. A total of 311 patients, 56% females, with a median age of 55 years (range: 23 to 87) participated in the study. Diabetes Score correlated with glycemic control, HbA1c (r = –0.20) and blood glucose (r = –0.25; P < 0.001), indicating v...

Frontiers in Psychology, 2021

Objectives: To determine the predictive association between fear of COVID-19 and emotional distre... more Objectives: To determine the predictive association between fear of COVID-19 and emotional distress (depression, anxiety, and stress) in frontline and non-frontline nurses. To explore the mediating role of socio-demographic features.Methods: Correlational cross-sectional research design was implied. A total of 500 on-duty male and female, frontline and non-frontline, nurses were included from five major hospitals in Gujrat (Aziz Bhatti Shaheed Hospital, City Hospital, Doctors Hospital, Akram Hospital, and Gujrat Hospital). Fear of COVID-19 scale and the Urdu version of depression, anxiety, and stress scale - 21 (DASS-21) were used to measure variables of interest. Descriptive statistics, structural equation modeling (SEM), linear regression, and t-test were carried out using Statistical Package for Social Sciences (SPSS) 21.Result: Structural equation modeling (SEM) revealed a significant predictive link between fear of COVID-19 and depression, anxiety, and stress (goodness of model...

SSRN Electronic Journal, 2020

International Journal of Insect Science, 2019

Citrus leafminer (CLM), Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae), is one of ... more Citrus leafminer (CLM), Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae), is one of the most important insect pests of Pakistan’s citrus nursery stock and caused extensive damage to young flushes. The organic compost is a widespread technique used to manage insect pests and plant diseases. Different composts (biofert, tara root and vermicompost) at 0.5 and 0.25 kg/plant concentration in comparison to NPK fertilizer at 0.4 and 0.2 g/plant were evaluated for CLM infestation and the associated citrus canker disease in nursery plantations of Citrus reticulata Blanco. Application of biofert at 0.5 kg/plant reduced the CLM infestation up to 54.5% during Fall-2016 and 39.1% during Summer-2017 in comparison to control treatment. The CLM larval density was also found lower by the application of biofert followed by vermicompost during both seasons. Both concentrations of biofert followed by vermicompost at 0.5 kg/plant resulted in remarkable protection against citrus canker disea...

International Journal of Emergency Mental Health and Human Resilience, 2016

Scientific Reports, 2016

Despite the importance of matrix rigidity on cell functions, many aspects of the mechanosensing p... more Despite the importance of matrix rigidity on cell functions, many aspects of the mechanosensing process in highly migratory cells remain elusive. Here, we studied the migration of highly motile keratocytes on culture substrates with similar biochemical properties and rigidities spanning the range between soft tissues (~kPa) and stiff culture substrates (~GPa). We show that morphology, polarization and persistence of motile keratocytes are regulated by the matrix stiffness over seven orders of magnitude, without changing the cell spreading area. Increasing the matrix rigidity leads to more F-actin in the lamellipodia and to the formation of mature contractile actomyosin fibers that control the cell rear retraction. Keratocytes remain rounded and form nascent adhesions on compliant substrates, whereas large and uniformly distributed focal adhesions are formed on fan-shaped keratocytes migrating on rigid surfaces. By combining poly-L-lysine, fibronectin and vitronectin coatings with selective blocking of α v β 3 or α 5 β 1 integrins, we show that α V β 3 integrins permit the spreading of keratocytes but are not sufficient for polarization and rigidity sensing that require the engagement of α 5 β 1 integrins. Our study demonstrates a matrix rigidity-dependent regulation of the directional persistence in motile keratocytes and refines the role of α v β 3 and α 5 β 1 integrins in the molecular clutch model. Cell migration is an essential process in embryonic development, wound repair, and immune responses resulting from multiple interactions among intracellular organelles 1. The directional movement of motile cells, which is critical to many physiological 2 and pathological 3 situations, is a highly integrated process guided by gradients of environmental cues. These cues may be diffusible or substrate-bounded, as in chemotaxis 4 and haptotaxis 5 respectively, or physical, including topography, 6 electric fields 7 or extracellular-matrix (ECM) rigidity 8. The directed migration of a cell along an ECM-rigidity gradient, known as "durotaxis", was originally observed in fibroblastic cells migrating along a soft-to-stiff interface 9. Although durotaxis is thought to be critical to development of the nervous system 10 , epithelial-to-mesenchymal transition 11 , or cancer metastasis 12 , the cellular machinery used by motile cells to sense matrix rigidity and to migrate towards stiffer zones remains poorly understood. Motile cells are thought to transmit forces to their surroundings by coupling their actin cytoskeleton to the ECM through adhesion sites 13. Low motile cells, in general, exhibit highly organized actin stress fibers and larger spreading areas on stiff substrate, both leading to an accumulation of internal prestress 14,15. Recent attentions to dynamic cellular processes on compliant environment have proposed the motor-clutch hypothesis 16. In this model, cell adhesion molecules act as molecular clutches that create a frictional slippage interface modulating the degree of force transmission from actomyosin fibers. However, the role of the ECM rigidity in the regulation of motile cell properties such as cell shape, polarization, speed and trajectories is poorly characterized. In addition, the identification of the precise molecular interactions involved in the regulation of the molecular clutch on compliant matrices is critical to further understanding the rigidity-sensing mechanism of motile cells.

Cell adhesion & migration, Jan 25, 2016

The mechanical properties of living cells reflect their propensity to migrate and respond to exte... more The mechanical properties of living cells reflect their propensity to migrate and respond to external forces. Both cellular and nuclear stiffnesses are strongly influenced by the rigidity of the extracellular matrix (ECM) through reorganization of the cyto- and nucleoskeletal protein connections. Changes in this architectural continuum affect cell mechanics and underlie many pathological conditions. In this context, an accurate and combined quantification of the mechanical properties of both cells and nuclei can contribute to a better understanding of cellular (dys-)function. To address this challenge, we have established a robust method for probing cellular and nuclear deformation during spreading and detachment from micropatterned substrates. We show that (de-)adhesion kinetics of endothelial cells are modulated by substrate stiffness and rely on the actomyosin network. We combined this approach with measurements of cell stiffness by magnetic tweezers to show that relaxation dynam...

médecine/sciences, 2016

Mechanobiology and soft matter group, laboratoire interfaces et fluides complexes, instituts de b... more Mechanobiology and soft matter group, laboratoire interfaces et fluides complexes, instituts de biosciences et matériaux,

Biophysical Journal, 2014

Methods in Cell Biology, 2014

This protocol describes a simple method to deposit protein micropatterns over a wide range of cul... more This protocol describes a simple method to deposit protein micropatterns over a wide range of culture substrate stiffness (three orders of magnitude) by using two complementary polymeric substrates. In the first part, we introduce a novel polyacrylamide hydrogel, called hydroxy-polyacrylamide (PAAm), that permits to surmount the intrinsically nonadhesive properties of polyacrylamide with minimal requirements in cost or expertize. We present a protocol for tuning easily the rigidity of &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;soft&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot; hydroxy-PAAm hydrogels between ~0.5 and 50 kPa and a micropatterning method to locally deposit protein micropatterns on these hydrogels. In a second part, we describe a protocol for tuning the rigidity of &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;stiff&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot; silicone elastomers between ~100 and 1000 kPa and printing efficiently proteins from the extracellular matrix. Finally, we investigate the effect of the matrix rigidity on the nucleus of primary endothelial cells by tuning the rigidity of both polymeric substrates. We envision that the complementarity of these two polymeric substrates, combined with an efficient microprinting technique, can be further developed in the future as a powerful mechanobiology platform to investigate in vitro the effect of mechanotransduction cues on cellular functions, gene expression, and stem cell differentiation.

Soft Matter, 2013

The nucleus has long been considered as a passive compartment containing the genetic information.... more The nucleus has long been considered as a passive compartment containing the genetic information. However, recent attention to its structure, mechanical properties and physical connections with other cellular compartments has shown that the nucleus changes dynamically its morphology and internal organization for important cellular processes, especially those associated with cellular confinement. In this paper, we review some recent progress in experimental investigations of nuclear squeezing that lead to a better understanding of the nuclear remodeling in response to various situations of cellular confinement. We will discuss compelling examples of original experiments performed with microsystems that have recently brought new insights into the close relationship between nuclear mechanics and cellular organization. We will show that the study of nuclear confinement with microsystems has opened up new experimental avenues that already offer promising clues for understanding diseases that are associated with defective nuclear mechanics.

Biophysical Journal, 2013

mechanism of cell migration in confined spaces is still unclear. Here we propose a jet propulsion... more mechanism of cell migration in confined spaces is still unclear. Here we propose a jet propulsion model of the cell motility when cells migrate through heterogeneous structures in tissues, such as lymphatic vessels, vasculature and nerves. We show that as long as the cell is polarized so that an ion concentration gradient along the elongated cell body can be maintained, water molecules can be ingested into the cell at the leading end and emitted at the trailing end of the cell. Therefore, cells can migrate forward at a high speed. Our model also predicts that an osmotic shock can reverse the direction of cell migration in microchannels. These predictions were verified by our microfluidic experiments. The effects of actin, mysoin, microtubules and ion transporters were studied theoretically and experimentally.