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Papers by masoud ghorbani

Journal of Consumer Protection and Food Safety, 2021

Toxoplasmosis is one of the most important zoonotic diseases with serious health risks for humans... more Toxoplasmosis is one of the most important zoonotic diseases with serious health risks for humans, especially for immunodeficient patients, and can lead to abortion in pregnant women worldwide. The oral uptake of sporulated oocysts and/or consumption of undercooked/raw meat of animals infected with Toxoplasma gondii can infect other animals and humans. Heart, liver, and meat tissues of 150 sheep and 150 goats from a slaughterhouse in Ahvaz, Iran, were collected during autumn 2018 and analyzed via polymerase chain reaction (PCR) to detect parasitic DNA in the animal tissues. Moreover, antibodies against T. gondii of 150 sera samples were detected as the targets by in-house enzyme-linked immunosorbent assay (in-house ELISA). A total of 26 (17.3%), 33 (22%), and 48 (32%) of liver, meat, and heart samples in sheep, and a total of 24 (16%), 26 (17.3%), and 36 (24%) of liver, meat, and heart samples in goats, respectively, showed positive PCR results. Besides, the ELISA evaluation of sera samples from 150 sheep and 150 goats resulted in 26 (13.3%) and 16 (10.6%) positive cases, respectively. A significant difference was also found between PCR-positive heart samples and ELISA-positive sera samples of both animal species (p < 0.05), but no significant difference existed between PCR-positive liver samples and ELISA-positive sera samples of both species (p > 0.05). The results of this study confirm the presence of T. gondii in sheep and goats' consumable organs, highlighting the need to avoid consuming raw or uncooked organs of these animal species to prevent human infection with T. gondii.

Journal of Applied Biotechnology Reports, 2019

Introduction Cartilage is a specialized connective tissue containing extracellular matrix (ECM) r... more Introduction Cartilage is a specialized connective tissue containing extracellular matrix (ECM) rich in GAG and proteoglycans. This matrix can cause the cartilage to withstand mechanical pressure without permanent deformation. Also, these compounds create an appropriate smoothness and sliding in joints. 1 Cartilage tissue does not have a high regenerative power due to its lack of blood flow unlike the bone, which has a more efficient mechanism for repairing itself. 1 The damaged or eroded cartilage has limited ability to repair itself. It has also less power in malicious and wider damage. 2-6 Nowadays, cartilage diseases such as osteoarthritis are the most common skeletal diseases. These diseases are especially common in older people or athletes. According to a report, over 39 million people over 25 years in Europe and 26 million people in America experience osteoarthritis and have estimated this number to be doubled by 2020. 7,8 Today, the treatment of cartilage diseases is one of the biggest problems in the medical society. A series of new therapies has been taken into consideration based on tissue engineering in recent years for the treatment of cartilage diseases. The procedure requires proper scaffold, repairing cells, and growth factors. Tissue engineering of cartilage using stem cells is to either produce cartilage in the laboratory or to produce cartilage producing cells in the body. In the occasion that the cartilage is synthesized in the laboratory, it must be transplanted into the body and in the joints. In this type of treatment, the patient will not need artificial joints anymore. Among the appropriate treatment

Background: Low back pain is a major economical and social problem nowadays. Intervertebral disc ... more Background: Low back pain is a major economical and social problem nowadays. Intervertebral disc herniation and central degeneration of disc are two major reasons of low back pain that occur because of structural impairment of discs. Intervertebral disc includes the annulus fibrosus, transitional region, and nucleus pulposus (NP). NP forms the central nucleus of the disc. Reduction of cell count and extracellular matrix, especially in NP, causes disc degeneration. Different scaffolds (natural and synthetic) have been used for tissue repairing and regeneration of intervertebral disc in tissue engineering. Most scaffolds have biodegradable and biocompatible characteristics and also prepare a fine condition for proliferation and migration of cells. Although no specific marker or method has been suggested for recognition of NP cells, some studies have used real time and immunocytochemical methods and reported high expression of cytokeratin 19, 18, 8, and others as markers for NP cells. This study aimed to recognize NP cells of human intervertebral disc by flow cytometry of cytokeratin 18 marker. It also compared the proliferation and morphology of these cells in chitosan-gelatin scaffold and alginate scaffold. Methods: NP cells were derived by enzymatic hydrolysis of collagenase from NP tissue of patients undergoing open surgery for discectomy in Alzahra Hospital (Isfahan, Iran). Chitosan was blended with gelatin and glutaraldehyde was used for cross linking of the two polymers. Then, alginate scaffold was prepared. After approving the NP cells by flow cytometry of cytokeratin 18 marker, a cellular suspension with 4 × 10 5 cells was transferred to each scaffold and cultured for 21 days. Cell viability and proliferation were investigated by trypan blue and methyl thiazolyl tetrazolium (MTT) assay. A scanning electron microscope (SEM) was used to assert the porosity and to survey the structures of the scaffolds. Findings: We can use flow cytometry of cytokeratin 18 markers for recognition of NP cells. MTT assay demonstrated that cell viability on the third day had significant difference with the first day in both scaffolds. There was also a significant reduction in cellular viability from day 3 to day 21. Results of cell count showed that mean difference between cell counts in alginate scaffold was significantly more than chitosan-gelatin scaffold (P < 0.001). Conclusion: Flow cytometry of cytokeratin 18 can be used as a method for recognition of NP cells. Compared to chitosan-gelatin scaffold, alginate scaffold prepared a better condition for proliferation of NP cells. The results of this study suggested that alginate scaffold could be useful in in-vivo studies and treatment.

Scientific Reports

Repairing of large bone injuries is an important problem in bone regeneration field. Thus, develo... more Repairing of large bone injuries is an important problem in bone regeneration field. Thus, developing new therapeutic approaches such as tissue engineering using 3D scaffolds is necessary. Incorporation of some bioactive materials and trace elements can improve scaffold properties. We made chitosan/alginate/strontium-doped bioglass composite scaffolds with optimized properties for bone tissue engineering. Bioglass (BG) and Sr-doped bioglasses (Sr-BG) were synthesized using Sol–Gel method. Alginate-Chitosan (Alg/Cs) scaffold and scaffolds containing different ratio (10%, 20% and 30%) of BG (Alg/Cs/BG10, 20, 30) or Sr-BG (Alg/Cs/Sr-BG10, 20, 30) were fabricated using freeze drying method. Characterization of bioglasses/scaffolds was done using zeta sizer, FTIR, XRD, (FE) SEM and EDS. Also, mechanical strength, antibacterial effect degradation and swelling profile of scaffolds were evaluated. Bone differentiation efficiency and viability of MSCs on scaffolds were determined by Alizarin...

ARYA atherosclerosis, 2013

Atherosclerosis is the main cause of cardiovascular disease which is caused by a high-fat diet. M... more Atherosclerosis is the main cause of cardiovascular disease which is caused by a high-fat diet. Many of these patients use boiled quince leaves for their treatment. However, the supporting scientific information is limit. The aim of this study was to evaluate the effect of quince leaf on the progression of atherosclerosis and whether it can be an appropriate alternative to statins. 24 male rabbits were randomly divided into two groups: normal diet (6 n) and high-cholesterol diet (2% cholesterol, 18 n) for 8 weeks. At the end of the 8 weeks, both groups underwent blood sampling and their biochemical markers were measured. Then, all animals in the normal-diet group and three of the high-cholesterol diet group were killed to investigate atheromic plaque in their coronary artery. The 15 remaining rabbits of the high-cholesterol diet group were randomly divided into 3 groups (5 n) after discontinuation of the fatty diet. The first group was not given any treatment, the second received at...

Journal of Pediatric Surgery, 2012

BACKGROUND: Stenosis of trachea with mucosal and cartilage lesions is a challenging problem in tr... more BACKGROUND: Stenosis of trachea with mucosal and cartilage lesions is a challenging problem in tracheal surgery. Owing to ease of harvest and abundance, adipose-derived stem cells (ADSCs) are attractive and increasingly used in tissue engineering. The aim of this study was to evaluate the repair of trachea with autologous stem cells and differentiated chondrocytes from adipose-derived stem cells in an animal model.

Nowadays high accident rates, fractures leading to permanent bone disorders and the impossibility... more Nowadays high accident rates, fractures leading to permanent bone disorders and the impossibility of bone transplant have made scientists to look for new methods of repairing injured bones. Considering the application of stem cells in bone tissue engineering, there exists the necessity to investigate various culture methods and suitable fields and scaffolds. Thus, we decided to induce adipose-derived stem cells into osteoblast cells in two systems of pellet culture and monolayer and compare osteogenic markers. Methods: Stem cells have been separated via mechanical and enzymatic methods and cultured in monolayer and pellet culture models with osteogenic medium. Then, RNA was separated from differentiated cells, complementary DNA (cDNA) was synthesized and amplified. Polymerase chain reaction (PCR) product was transferred to electrophoresis gel. The intensity of the bands was measured by Image-J software and analyzed by SPSS. Results: average osteopontin, osteocalcin and Runx2 genes in differentiated cells in the two culture systems showed a significant difference. The expression of osteocalcin, osteopontin and Runx2 gense in pellet system were more than monolayer systems in 21 days. Conclusion: This study indicated that pellet and monolayer culture systems are appropriate for bone engineering but osteocalcin, osteopontin and Runx2 genes expressions were different in the two culture system.

Background: Low back pain is an important disorder linked with degenerative changes in the interv... more Background: Low back pain is an important disorder linked with degenerative changes in the intervertebral disc (IVD). Degradation of IVD is caused by decreased number of cells, reduced production and degradation of extracellular matrix of IVD tissue especially in nucleus pulposus (NP). Natural and synthetic scaffolds are used for regeneration of IVD in tissue engineering. Aggrecan is an important proteoglycan in NP tissue of IVD. This study aimed to levels of aggrecan secreted by human NP cells of IVD in alginate and chitosan-gelatin scaffolds. Methods: Collagenase enzymatic hydrolysis was used to extract NP cells from NP tissue of patients with IVD hernia in Alzahra Hospital (Isfahan, Iran). Chitosan gel was mixed with gelatin gel and freeze dried to make the scaffold. An alginate scaffold was also prepared. Cellular suspension containing the extracted NP cells was transferred to each scaffold and cultured for 14 days. The levels of secreted aggrecan were investigated by enzyme-linked immunosorbent assay. A light microscope was used to assert the morphology of NP cells. Findings: Secretion of aggrecan had significant increases during the third to the 14 th day. The increments were more considerable in alginate scaffolds. There were significant differences in secreted aggrecan between alginate and chitosan scaffolds on the seventh and 14 th days. However, no such a significant difference was observed on the third day. The two scaffolds were significantly different in terms of the secretion of extracellular matrix by NP. Conclusion: Compared to the chitosan-gelatin scaffold, the alginate scaffold provided better conditions for aggrecan secretion in NP cells in vitro. The use of thus scaffold is suggested to culture NP cell in vivo.

Acta Scientific Pharmaceutical Sciences, 2021

Background: Spermatogenesis is the process that spermatogonial stem cells (SSCs) differentiate to... more Background: Spermatogenesis is the process that spermatogonial stem cells (SSCs) differentiate to spermatozoa in the testis seminiferous tubules. Effective in vitro differentiation of SSCs to sperm can be a promising sign for reconstruction of spermatogenesis disorders. This research was designed to evaluate the effect of a 3D nanofibrous scaffold on culture and differentiation of mouse SSCs. Materials and Methods: In this research, using electrospinning technique, a nanofibrous polycaprolactone (PCL) scaffold incorporated with multiwalled carbon nanotubes (MWCNTs) was fabricated. The nanofibrous PCL/MWCNTs were assessed using Scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR). Results: Then, the SSCs were seeded on the PCL/MWCNTs scaffolds and they had high survival rate and differentiated to subsequent cell lines. Also, molecular result demonstrated that the SSCs on the 3D scaffold overexpressed the C-kit and SYCP3 proteins. Conclusion: Finally, this research showed the synergistic effects of 3D scaffolds on proliferation and differentiation of SSCs.

Tissue and Cell, 2021

Infertility is one of the most common problems in the world that has negative effects on society ... more Infertility is one of the most common problems in the world that has negative effects on society and infertile people. Among the various causes of infertility, male infertility accounts for almost half of all infertility cases. Despite advances in medicine, current male infertility treatments such as assisted reproductive technology (ART) have not been successful in treating all types of male infertility. Recently, stem cells have been considered as therapeutic targets for many diseases, including infertility, due to their self-renewing and high differentiation. The purpose of this review is to discuss different types of male infertility and the effect of various stem cells against the treatment of male infertility.

Advanced Biomedical Research, 2015

How to cite this article: Karimi Z, Ghorbani M, Hashemibeni B, Bahramian H. Evaluation of the pro... more How to cite this article: Karimi Z, Ghorbani M, Hashemibeni B, Bahramian H. Evaluation of the proliferation and viability rates of nucleus pulposus cells of human intervertebral disk in fabricated chitosan-gelatin scaffolds by freeze drying and freeze gelation methods. Adv Biomed Res 2015;4:251.