mc200401886 MUHAMMAD ADEEL - Academia.edu (original) (raw)

Papers by mc200401886 MUHAMMAD ADEEL

Genes

Complex chromosomal rearrangements such as translocations play a critical role in oncogenesis. Tr... more Complex chromosomal rearrangements such as translocations play a critical role in oncogenesis. Translocation detection is vital to decipher their biological role in activating cancer-associated mechanisms. High-throughput chromosomal conformations capture (Hi-C) data have shown promising progress in unveiling the genome variations in a disease condition. Until now, multiple structural data (Hi-C)-based methods are available that can detect translocations in cancer genomes. However, the consistency and specificity of Hi-C-based translocation results still need to be validated with conventional methods. This study used Hi-C data of cancerous cell lines, namely lung cancer (A549), Chronic Myelogenous Leukemia (K562), and Acute Monocytic Leukemia (THP-1), to detect the translocations. The results were cross-validated through whole-genome sequencing (WGS) and paired-read analysis. Moreover, PCR amplification validated the presence of translocated reads in different chromosomes. By integr...

Molecules

Vicilin has nutraceutical potential and different noteworthy medicative health-promoting biotic d... more Vicilin has nutraceutical potential and different noteworthy medicative health-promoting biotic diversions, and it is remarkable against pathogenic microorganisms and insects. In this study, Vigna aconitifolia vicilin (VacV) has been identified and characterized from the seed of Vigna aconitifolia (Jacq.) Marechal (Moth beans). LC-MS/MS analysis of VacV provided seven random fragmented sequences comprising 238 residues, showing significant homology with already reported Vigna radiata vicilin (VraV). VacV was purified using ammonium sulfate precipitation (60%) followed by size exclusion chromatography on Hi-Load 16/60 Superdex 200 pg column and anion-exchange chromatography (Hi trap Q FF column). Purified VacV showed a major ~50 kDa band and multiple lower bands on 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under both reduced and non-reduced conditions. After all, a three-dimensional molecular structure of VacV was predicted, which showed β-sheeted molec...

Pharmaceuticals

Rift valley fever virus (RVFV) is the causative agent of a viral zoonosis that causes a significa... more Rift valley fever virus (RVFV) is the causative agent of a viral zoonosis that causes a significant clinical burden in domestic and wild ruminants. Major outbreaks of the virus occur in livestock, and contaminated animal products or arthropod vectors can transmit the virus to humans. The viral RNA-dependent RNA polymerase (RdRp; L protein) of the RVFV is responsible for viral replication and is thus an appealing drug target because no effective and specific vaccine against this virus is available. The current study reported the structural elucidation of the RVFV-L protein by in-depth homology modeling since no crystal structure is available yet. The inhibitory binding modes of known potent L protein inhibitors were analyzed. Based on the results, further molecular docking-based virtual screening of Selleckchem Nucleoside Analogue Library (156 compounds) was performed to find potential new inhibitors against the RVFV L protein. ADME (Absorption, Distribution, Metabolism, and Excretio...

Annals of Punjab Medical College (APMC), Aug 6, 2019

Objective: This retrospective cohort study presents simulation models for analyzing toxicities of... more Objective: This retrospective cohort study presents simulation models for analyzing toxicities of doxorubicin and docetaxel, both in combination of cyclophosphamide (AC and TC). It compared their side effects during postoperative chemotherapy of the treated Pakistani breast cancer patients.

Journal of Ayub Medical College Abbottabad, Mar 1, 2015

Background: Various approaches have been utilized in attempting to cryopreserve oocytes, beginnin... more Background: Various approaches have been utilized in attempting to cryopreserve oocytes, beginning with slow cooling and more recently the advent of technique of vitrification. Now it seems that oocyte cryopreservation is no longer an experimental technique and it is being increasingly utilized in clinics around the world. As successful outcome in oocyte cryopreservation can be assessed by survival through the freeze-thaw process, potential for fertilization, embryo development and dynamics of meiotic spindles. This study aimed to analyse these features in context of vitrification and slow freezing. Methods: In this laboratory based study, mature MII mouse oocytes from F1(C57BL6/J X CBA) mice (n=43) were divided randomly into two groups of equal numbers and were cryopreserved by slow freezing and by vitrification. Upon re-warming these oocytes were assessed for survival and for fertilization potential. Oocytes were fixed and stained to compare the effect of both protocols on spindle reassembly and chromosome configuration 10min, 1h and 3h after warming. Unfrozen oocytes were used as controls. Results: A greater number of vitrified oocytes survived cryopreservation than slow frozen oocytes (70.3% vs. 12.5%, p=0.024). After insemination, fertilization rates were higher for vitrified oocytes as compared to slow frozen oocytes (15.86% vs. 4.6%, p=0.046). Morphology of the meiotic spindle was found to be in a disorganized configuration in slow frozen oocytes at all-time points 10 mins, 1 h and 3h), whereas in vitrified oocytes the spindles were found to be aligned at all-time points. Chromosomes were seen to be displaced from equatorial region in both groups. Conclusion: Cryopreservation of mouse oocytes was conducted with greater success using vitrification, compared to slow freezing, with survival, fertilization, and spindle assembly more favourable to a successful outcome in this model.

Journal of Integrative Agriculture, 2022

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Sustainable Business and Society in Emerging Economies, 2020

Current study empirically analyzes bank specific factors and macroeconomic factors that determine... more Current study empirically analyzes bank specific factors and macroeconomic factors that determine the liquidity reserves of banks functioning in Pakistan. To highlight the association, current study performed random effects estimates on a data set of 20 banks from 2006 to 2016. Bank specific factors include bank size, capital and credit Risk. GDP and Inflation are the macroeconomic factors that were considered. Market competition has been measured through HHI. Based on panel data analysis, current study suggests that bank specific factors (except capital), macroeconomic factors and market competition significantly affect liquidity reserves of banks in Pakistan. These factors include bank size, credit risk, market competition, GDP and inflation. In addition, bank size, credit risk, GDP and Inflation revealed a negative effect on bank liquidity. On the other hand, market competition revealed a positive effect on bank liquidity. Capital showed an insignificant effect on bank liquidity.

J. Wirel. Mob. Networks Ubiquitous Comput. Dependable Appl., 2012

Over the years, wireless enabled mobile devices have become an important part of our daily activi... more Over the years, wireless enabled mobile devices have become an important part of our daily activities that can provide rich contextual information about the location and environment of an individual (for example who is in your proximity? and where are you?). Advancement in technology has opened several horizons to analyse and model this contextual information for human behaviour understanding. Objective of this research work is to utilise this information from wireless proximity data to find repeated patterns in daily life activities and individual behaviours. These repeated patterns can give information about the unusual activities and behaviour of an individual. To validate and further investigate this concept, we used Bluetooth proximity data in this paper. Repeated activity patterns and behaviour of low entropy mobile people are detected by using two different techniques, N-gram and correlative matrix techniques. Primary purpose was to find out whether contextual information obt...

Journal of Ayub Medical College, Abbottabad : JAMC, 2020

BACKGROUND Various treatment modalities have been used in treating femoral shaft fractures, i.e.,... more BACKGROUND Various treatment modalities have been used in treating femoral shaft fractures, i.e., open intramedullary nailing, plating and external fixators but these does not always produce stable fixation and there is increased risk of infection, closed locked intramedullary nailing technique has being the gold standard and is a routine procedure but it requires proper orthopedic operation table with traction apparatus and the use of image intensifier. The use of open interlocking nailing technique doesn't require any special orthopaedic table nor it requires use of Image intensifier. The objective of this study is to find out and determine the frequency of fracture union and wound infection in open reamed interlocking nailing of close fractures of shaft of femur. METHODS This study included fifty-eight patients from either gender, above 14 years of age with closed femoral shaft fracture presenting within 2 weeks. Data was collected on Performa about gender, age, address, date...

This is a review article discussing the progress in using micro-doppler based features for target... more This is a review article discussing the progress in using micro-doppler based features for target classification.

Tema. Journal of Land Use, Mobility and Environment, 2018

This paper examines the 2007 national time use survey to report the degree to which the national ... more This paper examines the 2007 national time use survey to report the degree to which the national travel behaviour varies between urban and rural of Pakistan and how it is shaped by the local socioeconomic and individual characteristics in these areas. At the national level, walking remains the dominant mode of daily mobility across the country. However, the daily trip rate, mode choice and travel durations vary significantly across both geographies. Urban residents are slightly less mobile and exhibits greater use of personal automobile than rural residents. These differences become more pronounced across gender. There exists slight local regional variation across provinces which are closely related to the local social and spatial drivers of mobility. The paper speculates that the rural travel differences are mainly caused by difference in income levels. Urban built environment is more conducive to motorized mobility which results in greater automobile reliance in cities, particular...

SUMMARY Cadastre plays the central role for land administration and record management in Pakistan... more SUMMARY Cadastre plays the central role for land administration and record management in Pakistan. There are approximately 190 million land records containing the details of approximately 50 million landowners in Pakistan. All the land record cadastres are prepared and maintained in manual form. Although this land administration system is quite scientific in theory, in practice the system is a mess of traditional approaches and practices which cannot meet the present day demands. The study evaluates the advantages and disadvantages of present mapping environment and map outputs. It identifies that modern technologies like Remote sensing and Geo spatial Information Systems can be used to increase the accuracy of the cadastral contents leading to improved tenure security of the general public. The study also recommends that the cadastre map data needs to be linked with the mapping output of the survey of Pakistan. The step will help achieving a multipurpose cadastre and will ensure sp...

Annals of King Edward Medical University, 2017

Background: Carpal tunnel syndrome (CTS) is the most commonly known entrapment neuropathy in whic... more Background: Carpal tunnel syndrome (CTS) is the most commonly known entrapment neuropathy in which median nerve of the body is compressed or traumatized. The problem is described by numbness and tingling sensation in the region of tangible dispersion of the nerve in the hand that is joined by torment as well as shortcoming of thumb muscles. The incidence rate of CTS is 139/100,000 per year for men and 506/100,000 per year for women. Objective: To compare the difference in pre/post numeric pain rating scale scores (NPRS) and Boston Carpal Tunnel Syndrome Questionnaire Scores (BCTSQ) with the use of therapeutic ultrasound versus local steroid injection in the treatment of mild to moderate carpal tunnel syndrome. Methodology: A randomized clinical trial was done at Mayo Hospital Lahore, after the approval of synopsis from March 2016 to August 2016.The sample size of 60 patients (30 subjects in every group) was assessed by utilizing 5% level of significance, 90% power of test with expec...

The synthesis of p-n heterojunction has received much attention in the eradication of organic pol... more The synthesis of p-n heterojunction has received much attention in the eradication of organic pollutants from water. In this study, p-n CoO-ZnO heterojunction is reported as an efficient visible-light-driven photocatalyst for degradation of methylene blue. The formation of CoO-ZnO was determined by XRD, XPS, UV-vis DRS, SEM, and EDS analyses. The p-n CoO-ZnO prepared by precipitation method showed an enhanced photocatalytic activity under the irradiation of visible light compared to ZnO. A 100 mg/L methylene blue is degraded almost completely within 2 hours under visible irradiation. The enhanced photocatalytic efficiency is attributed to the synergistic effect of p-n heterojunction that dramatically reduces the recombination of holes and electrons initiated by irradiation. The role played by hydroxyl radicals in photodegradation of methylene blue was determined by photoluminescence emission and fluorescence spectroscopy.

Nanomaterials, 2021

The ubiquitous presence of microplastics (MPs) and nanoplastics (NPs) in the environment is an un... more The ubiquitous presence of microplastics (MPs) and nanoplastics (NPs) in the environment is an undeniable and serious concern due to their higher persistence and extensive use in agricultural production. This review highlights the sources and fate of MPs and NPs in soil and their uptake, translocation, and physiological effects in the plant system. We provide the current snapshot of the latest reported studies with the majority of literature spanning the last five years. We draw attention to the potential risk of MPs and NPs in modern agriculture and their effects on plant growth and development. We also highlight their uptake and transport pathways in roots and leaves via different exposure methods in plants. Conclusively, agricultural practices, climate changes (wet weather and heavy rainfall), and soil organisms play a major role in transporting MPs and NPs in soil. NPs are more prone to enter plant cell walls as compared to MPs. Furthermore, transpiration pull is the dominant fa...

Polymer, 2021

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

ACS Omega, 2021

High-grade serous ovarian cancer (HGSOC) is one of the major lifethreatening cancers in women, wi... more High-grade serous ovarian cancer (HGSOC) is one of the major lifethreatening cancers in women, with a survival rate of less than 50%. So far, chemotherapy is the main therapeutic tool to cure this lethal disease; however, in many cases, it fails to cure HGSOC even with severe side effects. Self-therapeutic nanomaterials could be an effective alternative to chemotherapy, facilitated by their diverse physicochemical properties and the ability to generate reactive species for killing cancer cells. Herein, inorganic cobalt hydroxide nanosheets (Co(OH) 2 NS) were synthesized by a simple solution process at room temperature, and morphological, spectroscopic, and crystallographic analyses revealed the formation of Co(OH) 2 NS with good crystallinity and purity. The as-prepared Co(OH) 2 NS showed excellent potency, comparable to the FDA-approved cisplatin drug to kill ovarian cancer cells. Flow cytometric analysis (nnexin V) revealed increased cellular apoptosis for Co(OH) 2 NS than cobalt acetate (the precursor). Tracking experiments demonstrated that Co(OH) 2 NS are internalized through the lysosome pathway, although relocalization in the cytoplasm has been observed. Hence, Co(OH) 2 NS could be an effective self-therapeutic drug and open up an area for the optimization of self-therapeutic properties of cobalt nanomaterials for cancer treatment.

Coherent detected phase-OTDR (CDPO) systems are mainly used in distributed vibration sensing and ... more Coherent detected phase-OTDR (CDPO) systems are mainly used in distributed vibration sensing and these systems are preferred over the direct detected phase-OTDR (DDPO) systems due to their inherent property of mitigating a certain type of phase noise. There is a possibility that the existing non-mitigated phase noise can be controlled in CDPO systems only after properly investigating the characteristics of both the CDPO mitigated and CDPO-non-mitigated noise types. Hence, the first and crucial step, i.e, the characteristics of two different phase noise types are determined in this paper. This determination will help in providing ease about finding the real sources of both of the phase noise types and hence can help in mitigating the existing phase noise further.