meriem Baouche - Academia.edu (original) (raw)

Papers by meriem Baouche

Research paper thumbnail of Feline herpesvirus 1 (FHV-1) enters the cell by receptor-mediated endocytosis

Journal of Virology

Feline herpesvirus type 1 (FHV-1) is an enveloped dsDNA virus belonging to the Herpesviridae fami... more Feline herpesvirus type 1 (FHV-1) is an enveloped dsDNA virus belonging to the Herpesviridae family and is considered one of the two primary viral etiological factors of feline upper respiratory tract disease. In this study, we investigated the entry of FHV-1 into host cells using two models: the AK-D cell line and primary feline skin fibroblasts (FSFs). We employed confocal microscopy, siRNA silencing, and selective inhibitors of various entry pathways. Our observations revealed that the virus enters cells via pH and dynamin-dependent endocytosis, as the infection was significantly inhibited by NH 4 Cl, bafilomycin A1, dynasore, and mitmab. Additionally, genistein, nystatin, and filipin treatments, siRNA knock-down of caveolin-1, as well as FHV-1 and caveolin-1 colocalization suggest the involvement of caveolin-mediated endocytosis during the entry process. siRNA knock-down of clathrin heavy chain and analysis of virus particle colocalization with clathrin indicated that clathrin-m...

Research paper thumbnail of Feline Wharton’s jelly-derived mesenchymal stem cells as a feeder layer for oocytes maturation and embryos culture in vitro

Frontiers in Veterinary Science

IntroductionDue to their capacity to release growth factors and cytokines, co-culture using mesen... more IntroductionDue to their capacity to release growth factors and cytokines, co-culture using mesenchymal stem cells has been considered a good alternative to promoting the maturation of the oocytes and the embryo’s development quality in vitro in different mammalian species. In this regard, we investigated the effect of feline Wharton’s jelly MSCs as feeders layer in oocyte maturation—consequently, the development of resulting embryos in co-culture.MethodsOocytes with dark cytoplasm and a few layers of cumulus cells were collected and subjected to in vitro maturation and embryo culture using commercial media with and without MSCs addition. The oocytes’ nuclear maturation and the degree of cumulus expansion in different groups were assessed after 24 h; the development of the embryo was evaluated every 12 h until day eight.ResultsAlthough MSCs increased the proportion of cumulus cells oocytes exhibiting cumulus expansion, there were no significant differences in the percentage of matur...

Research paper thumbnail of Mesenchymal Stem Cells: Generalities and Clinical Significance in Feline and Canine Medicine

Animals

Mesenchymal stem cells (MSCs) are multipotent cells: they can proliferate like undifferentiated c... more Mesenchymal stem cells (MSCs) are multipotent cells: they can proliferate like undifferentiated cells and have the ability to differentiate into different types of cells. A considerable amount of research focuses on the potential therapeutic benefits of MSCs, such as cell therapy or tissue regeneration, and MSCs are considered powerful tools in veterinary regenerative medicine. They are the leading type of adult stem cells in clinical trials owing to their immunosuppressive, immunomodulatory, and anti-inflammatory properties, as well as their low teratogenic risk compared with pluripotent stem cells. The present review details the current understanding of the fundamental biology of MSCs. We focus on MSCs’ properties and their characteristics with the goal of providing an overview of therapeutic innovations based on MSCs in canines and felines.

Research paper thumbnail of Feline umbilical cord mesenchymal stem cells: Isolation and in vitro characterization from distinct parts of the umbilical cord

Theriogenology, Dec 1, 2022

Research paper thumbnail of Cladophora glomerata methanolic extract promotes chondrogenic gene expression and cartilage phenotype differentiation in equine adipose-derived mesenchymal stromal stem cells affected by metabolic syndrome

Stem Cell Research & Therapy

Background Chondrogenesis represents a highly dynamic cellular process that leads to the establis... more Background Chondrogenesis represents a highly dynamic cellular process that leads to the establishment of various types of cartilage. However, when stress-related injuries occur, a rapid and efficient regeneration of the tissues is necessary to maintain cartilage integrity. Mesenchymal stem cells (MSCs) are known to exhibit high capacity for self-renewal and pluripotency effects, and thus play a pivotal role in the repair and regeneration of damaged cartilage. On the other hand, the influence of certain pathological conditions such as metabolic disorders on MSCs can seriously impair their regenerative properties and thus reduce their therapeutic potential. Objectives In this investigation, we attempted to improve and potentiate the in vitro chondrogenic ability of adipose-derived mesenchymal stromal stem cells (ASCs) isolated from horses suffering from metabolic syndrome. Methods Cultured cells in chondrogenic-inductive medium supplemented with Cladophora glomerata methanolic extrac...

Research paper thumbnail of Etude du portage digestif des souches de bacilles à Gram négatif productrices de carbapénèmases isolées aux CHU Khelil Amrane

Liste des tableaux Tableau I : Antibiotiques testés………………………………………………………....08 Tableau II. Interp... more Liste des tableaux Tableau I : Antibiotiques testés………………………………………………………....08 Tableau II. Interprétation des résultats du Carba NP test modifié …………..….09 Tableau III : caractéristiques des patients prélever………....…………………..….11 Tableau IV : Résultats de la sensibilité aux antibiotiques ……..……………….…14. Les β-lactamines représentent la principale famille d'antibiotiques la plus développée et la plus utilisée dans le monde. Cette large utilisation est due à leur large spectre d'action, et à leur faible toxicité (Handal et al, 2000). La base commune à toutes les bêta-lactamines est le noyau azétidinone qui contient la structure carbonyle lactame la quelle est indispensable pour l'activité des molécules. À partir de cette structure, quatre groupes ont été développées par adjonction d'un cycle : les pénames, les céphèmes, les pénèmes ou par substitution, les monobactames. Les inhibiteurs des β-lactamases appartiennent également à cette famille (Bryskier., 1999).

Research paper thumbnail of Cladophora glomerata enriched by biosorption with Mn(II) ions alleviates lipopolysaccharide‐induced osteomyelitis‐like model in MC3T3‐E1, and 4B12 osteoclastogenesis

Journal of Cellular and Molecular Medicine

Research paper thumbnail of Feline herpesvirus 1 (FHV-1) enters the cell by receptor-mediated endocytosis

Journal of Virology

Feline herpesvirus type 1 (FHV-1) is an enveloped dsDNA virus belonging to the Herpesviridae fami... more Feline herpesvirus type 1 (FHV-1) is an enveloped dsDNA virus belonging to the Herpesviridae family and is considered one of the two primary viral etiological factors of feline upper respiratory tract disease. In this study, we investigated the entry of FHV-1 into host cells using two models: the AK-D cell line and primary feline skin fibroblasts (FSFs). We employed confocal microscopy, siRNA silencing, and selective inhibitors of various entry pathways. Our observations revealed that the virus enters cells via pH and dynamin-dependent endocytosis, as the infection was significantly inhibited by NH 4 Cl, bafilomycin A1, dynasore, and mitmab. Additionally, genistein, nystatin, and filipin treatments, siRNA knock-down of caveolin-1, as well as FHV-1 and caveolin-1 colocalization suggest the involvement of caveolin-mediated endocytosis during the entry process. siRNA knock-down of clathrin heavy chain and analysis of virus particle colocalization with clathrin indicated that clathrin-m...

Research paper thumbnail of Feline Wharton’s jelly-derived mesenchymal stem cells as a feeder layer for oocytes maturation and embryos culture in vitro

Frontiers in Veterinary Science

IntroductionDue to their capacity to release growth factors and cytokines, co-culture using mesen... more IntroductionDue to their capacity to release growth factors and cytokines, co-culture using mesenchymal stem cells has been considered a good alternative to promoting the maturation of the oocytes and the embryo’s development quality in vitro in different mammalian species. In this regard, we investigated the effect of feline Wharton’s jelly MSCs as feeders layer in oocyte maturation—consequently, the development of resulting embryos in co-culture.MethodsOocytes with dark cytoplasm and a few layers of cumulus cells were collected and subjected to in vitro maturation and embryo culture using commercial media with and without MSCs addition. The oocytes’ nuclear maturation and the degree of cumulus expansion in different groups were assessed after 24 h; the development of the embryo was evaluated every 12 h until day eight.ResultsAlthough MSCs increased the proportion of cumulus cells oocytes exhibiting cumulus expansion, there were no significant differences in the percentage of matur...

Research paper thumbnail of Mesenchymal Stem Cells: Generalities and Clinical Significance in Feline and Canine Medicine

Animals

Mesenchymal stem cells (MSCs) are multipotent cells: they can proliferate like undifferentiated c... more Mesenchymal stem cells (MSCs) are multipotent cells: they can proliferate like undifferentiated cells and have the ability to differentiate into different types of cells. A considerable amount of research focuses on the potential therapeutic benefits of MSCs, such as cell therapy or tissue regeneration, and MSCs are considered powerful tools in veterinary regenerative medicine. They are the leading type of adult stem cells in clinical trials owing to their immunosuppressive, immunomodulatory, and anti-inflammatory properties, as well as their low teratogenic risk compared with pluripotent stem cells. The present review details the current understanding of the fundamental biology of MSCs. We focus on MSCs’ properties and their characteristics with the goal of providing an overview of therapeutic innovations based on MSCs in canines and felines.

Research paper thumbnail of Feline umbilical cord mesenchymal stem cells: Isolation and in vitro characterization from distinct parts of the umbilical cord

Theriogenology, Dec 1, 2022

Research paper thumbnail of Cladophora glomerata methanolic extract promotes chondrogenic gene expression and cartilage phenotype differentiation in equine adipose-derived mesenchymal stromal stem cells affected by metabolic syndrome

Stem Cell Research & Therapy

Background Chondrogenesis represents a highly dynamic cellular process that leads to the establis... more Background Chondrogenesis represents a highly dynamic cellular process that leads to the establishment of various types of cartilage. However, when stress-related injuries occur, a rapid and efficient regeneration of the tissues is necessary to maintain cartilage integrity. Mesenchymal stem cells (MSCs) are known to exhibit high capacity for self-renewal and pluripotency effects, and thus play a pivotal role in the repair and regeneration of damaged cartilage. On the other hand, the influence of certain pathological conditions such as metabolic disorders on MSCs can seriously impair their regenerative properties and thus reduce their therapeutic potential. Objectives In this investigation, we attempted to improve and potentiate the in vitro chondrogenic ability of adipose-derived mesenchymal stromal stem cells (ASCs) isolated from horses suffering from metabolic syndrome. Methods Cultured cells in chondrogenic-inductive medium supplemented with Cladophora glomerata methanolic extrac...

Research paper thumbnail of Etude du portage digestif des souches de bacilles à Gram négatif productrices de carbapénèmases isolées aux CHU Khelil Amrane

Liste des tableaux Tableau I : Antibiotiques testés………………………………………………………....08 Tableau II. Interp... more Liste des tableaux Tableau I : Antibiotiques testés………………………………………………………....08 Tableau II. Interprétation des résultats du Carba NP test modifié …………..….09 Tableau III : caractéristiques des patients prélever………....…………………..….11 Tableau IV : Résultats de la sensibilité aux antibiotiques ……..……………….…14. Les β-lactamines représentent la principale famille d'antibiotiques la plus développée et la plus utilisée dans le monde. Cette large utilisation est due à leur large spectre d'action, et à leur faible toxicité (Handal et al, 2000). La base commune à toutes les bêta-lactamines est le noyau azétidinone qui contient la structure carbonyle lactame la quelle est indispensable pour l'activité des molécules. À partir de cette structure, quatre groupes ont été développées par adjonction d'un cycle : les pénames, les céphèmes, les pénèmes ou par substitution, les monobactames. Les inhibiteurs des β-lactamases appartiennent également à cette famille (Bryskier., 1999).

Research paper thumbnail of Cladophora glomerata enriched by biosorption with Mn(II) ions alleviates lipopolysaccharide‐induced osteomyelitis‐like model in MC3T3‐E1, and 4B12 osteoclastogenesis

Journal of Cellular and Molecular Medicine