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Journal of Applied Pharmaceutical Science

The present study evaluates the chemical composition and antimicrobial activity of essential oils... more The present study evaluates the chemical composition and antimicrobial activity of essential oils (EOs) of Juniperus Phoenicea of five localities from eastern Algeria. The analysis and identification of the components of the Eos was performed using the (GC-MS). The average yield of essential oil of the samples is 0.82%. The chemical composition of the EOs of J. Phoenicea is dominated by the presence of a major product, α-pinene (36.3-55.9%). Three components are represented with large concentrations, terpinolene (0-13%), Δ3-carene (0-12.4%) and the β-phellandrene (0-7.3%). Our investigation allows us to support the species Juniperus phoenicea of eastern Algeria has several variability quantitative and qualitative. The antimicrobial activity of the essential oils of J. phoenicea was evaluated against nine bacteria. The results showed a variable degree of antibacterial activity being the population Elhadjaz most effective.

Natural product communications, 2011

Essential oils extracted from dried leaves of Cupressus dupreziana A. Camus, an endemic species i... more Essential oils extracted from dried leaves of Cupressus dupreziana A. Camus, an endemic species in the Tassili n'Ajjer (Central Sahara of Algeria), were analyzed by gas chomatography coupled to mass spectrometry (GC-MS). Analyses were carried out on 164 trees of 26 natural populations in order to determine the intra-specific variability. Thirty-two terpenoids were identified, the major ones being alpha-pinene (11.5-44.2), delta3-carene (5.7-31.7) and germacrene-D (15.7-54.1). The terpenoid markers used made it possible to determine the individual patterns of chemotypic variability. This variability confirmed that genetic factors were not responsible for the decrease in the number of this species, the main reason probably being the Tassili n'Ajjer desertification.

Forest Research: Open Access, 2012

Essential oils extracted from dried leaves of Cupressus dupreziana A. Camus, an endemic species i... more Essential oils extracted from dried leaves of Cupressus dupreziana A. Camus, an endemic species in the Tassili n'Ajjer (Sahara Central Algeria), were analysed by gas chomatography coupled to mass spectrometry (GC-MS). Terpinoid analyses were performed on 13 trees in the natural population of Hahadjerine in order to determine the intra-population variability. 39 trepenoids were identified; the averages of the principal components were transtotarol (24.4%), Manoyl oxide (21.2%), α-pinene (15%) and Δ 3-carene (11.3%). The terpenoid markers used made it possible to determine the individual patterns of chemotypic variability. This variability confirms that genetic factors are not solely responsible for the decrease in the numbers of this species.

Phytothérapie, 2012

Le massif d'Ouanougha abrite une végétation forestière à plus de m d'altitude. Le cortège florist... more Le massif d'Ouanougha abrite une végétation forestière à plus de m d'altitude. Le cortège floristique de cette forêt à Cedrus atlantica et Quercus rotundifolia est très riche et comporte beaucoup d'espèces présentant un intérêt économique indéniable. La préservation et la valorisation de ce patrimoine naturel nécessitent un inventaire de la flore existante et des enquêtes ethnobotaniques.

Asian Journal of Plant Sciences, 2009


Asian Journal of Plant Sciences, 2007

The analysis and identification of the components of the essential oil of five populations of Jun... more The analysis and identification of the components of the essential oil of five populations of Juniperus Phoenicea, from Eastern Algeria, was performed using the GC-MS. The average yield of essential oil of the samples is 0.82%, the highest rate is observed in the essential oil of T'kout and Elhadjaz populations (0.92%), while the population of Menâa is characterised by the lowest yield (0.70%). These analyses led to the identification of 72 components. The chemical composition of the essential oil of J. Phoenicea is dominated by the presence of a major product, α-pinene (36.3-55.9%). Three components are represented with large concentrations in the essential oil; terpinolene (12.95%), Δ3-carene (0-12.38%) and the β-phellandrene (0-7.3%). Our investigation allows us to support the presence of several chemotypes in J. Phoenicea. The chemotype to Terpinolene is located in the region of Boussâada; T'kout is the region that has favoured the development of Δ3carene chemotype. The chemotype in β-phellandrene is located in the Boutaleb region.

The hydrodistilation of Centaurea solstitialis, gave a yield of 0.35%. The chemical analysis of t... more The hydrodistilation of Centaurea solstitialis, gave a yield of 0.35%. The chemical analysis of the essential oil by GC/MS, has allowed identifying 41 compounds corresponding to 99.37% of the total oil. The major compounds of the oil are n-heneicosane (17.30%), hexadecanoic acid (12.79%), n-tricosane (10.51%), n-pentacosane (5.64%) and caryophyllene oxide (5.03%). The antibacterial activity of the oil was tested on nine bacterial strains. The essential oil of this species has a moderate to significant antibacterial activity. The caryological study of C. solstitialis has allowed us to identify a karyotype with 2n = 2x = 18 chromosomes. The basic number x = 9 is reported for the first time in Algeria and Western Mediterranean clade.

The hydro-distillation of the essential oil of Rosmarinus officinalis gave a viscous liquid with ... more The hydro-distillation of the essential oil of Rosmarinus officinalis gave a viscous liquid with a whitish colour. The average yield of essential oil of the samples is 0.23%. This investigation allows us to support that R. officinalis includes six chemotypes in eastern Algerian. The difference of these chemotypes variants is the concentration of Eucalyptol, campene, α-pinene and camphor. The antibacterial activity of the essential oils chemotypes was evaluated against nine bacteria (Enterobacter cloacae ATCC 13047, MRSA (Methicillin-resistant Staphylococcus aureus), Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas syringae, Salmonella sp, Serratia liquefaciens ATCC 27592, Serratia marcescens ATCC 14756 and Shigella sp). The results showed that the essential oils have inhibited growth of bacterial strains. The essential oils chemotypes of Kherrata and the Bibans regions, generally exhibit antibacterial activity against the microorganisms' tested. The camphor chemotype with three variants exhibits a moderate antibacterial activity.

The analysis and identification of the essential oil components of six Rosmarinus officinalis pop... more The analysis and identification of the essential oil components of six Rosmarinus officinalis populations, from Eastern Algeria, was performed using GC-MS. The average yield of essential oil samples is 0.23%, the highest rate was observed in the essential oil of Kherrata population (0.35%), while Agmeroual population was characterised by the lowest yield (0.10%). These analyses led to the identification of 43 components. The chemical composition of the essential oil was dominated by the presence of major products, camphor (9.1-42.7%), eucalyptol (6.6-42.2%), α-pinene (11.4-25.2%), camphene (5.3-17.7%) and borneol (0.9-11.9%). Seven components were represented with average rates more than 1% in the essential oil, β-pinene, para-cymene, limonene, linalool, terpinene-4-ol, α-terpineol and β-caryophyllene. This investigation allows us to support that the species Rosmarinus officinalis of eastern Algeria includes several chemotypes. The chemotype to (eucalyptol, Camphor, α-pinene and camphene) was located in the regions of Kherrata and Bibans.

Journal of Applied Pharmaceutical Science

The present study evaluates the chemical composition and antimicrobial activity of essential oils... more The present study evaluates the chemical composition and antimicrobial activity of essential oils (EOs) of Juniperus Phoenicea of five localities from eastern Algeria. The analysis and identification of the components of the Eos was performed using the (GC-MS). The average yield of essential oil of the samples is 0.82%. The chemical composition of the EOs of J. Phoenicea is dominated by the presence of a major product, α-pinene (36.3-55.9%). Three components are represented with large concentrations, terpinolene (0-13%), Δ3-carene (0-12.4%) and the β-phellandrene (0-7.3%). Our investigation allows us to support the species Juniperus phoenicea of eastern Algeria has several variability quantitative and qualitative. The antimicrobial activity of the essential oils of J. phoenicea was evaluated against nine bacteria. The results showed a variable degree of antibacterial activity being the population Elhadjaz most effective.

Natural product communications, 2011

Essential oils extracted from dried leaves of Cupressus dupreziana A. Camus, an endemic species i... more Essential oils extracted from dried leaves of Cupressus dupreziana A. Camus, an endemic species in the Tassili n'Ajjer (Central Sahara of Algeria), were analyzed by gas chomatography coupled to mass spectrometry (GC-MS). Analyses were carried out on 164 trees of 26 natural populations in order to determine the intra-specific variability. Thirty-two terpenoids were identified, the major ones being alpha-pinene (11.5-44.2), delta3-carene (5.7-31.7) and germacrene-D (15.7-54.1). The terpenoid markers used made it possible to determine the individual patterns of chemotypic variability. This variability confirmed that genetic factors were not responsible for the decrease in the number of this species, the main reason probably being the Tassili n'Ajjer desertification.

Forest Research: Open Access, 2012

Essential oils extracted from dried leaves of Cupressus dupreziana A. Camus, an endemic species i... more Essential oils extracted from dried leaves of Cupressus dupreziana A. Camus, an endemic species in the Tassili n'Ajjer (Sahara Central Algeria), were analysed by gas chomatography coupled to mass spectrometry (GC-MS). Terpinoid analyses were performed on 13 trees in the natural population of Hahadjerine in order to determine the intra-population variability. 39 trepenoids were identified; the averages of the principal components were transtotarol (24.4%), Manoyl oxide (21.2%), α-pinene (15%) and Δ 3-carene (11.3%). The terpenoid markers used made it possible to determine the individual patterns of chemotypic variability. This variability confirms that genetic factors are not solely responsible for the decrease in the numbers of this species.

Phytothérapie, 2012

Le massif d'Ouanougha abrite une végétation forestière à plus de m d'altitude. Le cortège florist... more Le massif d'Ouanougha abrite une végétation forestière à plus de m d'altitude. Le cortège floristique de cette forêt à Cedrus atlantica et Quercus rotundifolia est très riche et comporte beaucoup d'espèces présentant un intérêt économique indéniable. La préservation et la valorisation de ce patrimoine naturel nécessitent un inventaire de la flore existante et des enquêtes ethnobotaniques.

Asian Journal of Plant Sciences, 2009


Asian Journal of Plant Sciences, 2007

The analysis and identification of the components of the essential oil of five populations of Jun... more The analysis and identification of the components of the essential oil of five populations of Juniperus Phoenicea, from Eastern Algeria, was performed using the GC-MS. The average yield of essential oil of the samples is 0.82%, the highest rate is observed in the essential oil of T'kout and Elhadjaz populations (0.92%), while the population of Menâa is characterised by the lowest yield (0.70%). These analyses led to the identification of 72 components. The chemical composition of the essential oil of J. Phoenicea is dominated by the presence of a major product, α-pinene (36.3-55.9%). Three components are represented with large concentrations in the essential oil; terpinolene (12.95%), Δ3-carene (0-12.38%) and the β-phellandrene (0-7.3%). Our investigation allows us to support the presence of several chemotypes in J. Phoenicea. The chemotype to Terpinolene is located in the region of Boussâada; T'kout is the region that has favoured the development of Δ3carene chemotype. The chemotype in β-phellandrene is located in the Boutaleb region.

The hydrodistilation of Centaurea solstitialis, gave a yield of 0.35%. The chemical analysis of t... more The hydrodistilation of Centaurea solstitialis, gave a yield of 0.35%. The chemical analysis of the essential oil by GC/MS, has allowed identifying 41 compounds corresponding to 99.37% of the total oil. The major compounds of the oil are n-heneicosane (17.30%), hexadecanoic acid (12.79%), n-tricosane (10.51%), n-pentacosane (5.64%) and caryophyllene oxide (5.03%). The antibacterial activity of the oil was tested on nine bacterial strains. The essential oil of this species has a moderate to significant antibacterial activity. The caryological study of C. solstitialis has allowed us to identify a karyotype with 2n = 2x = 18 chromosomes. The basic number x = 9 is reported for the first time in Algeria and Western Mediterranean clade.

The hydro-distillation of the essential oil of Rosmarinus officinalis gave a viscous liquid with ... more The hydro-distillation of the essential oil of Rosmarinus officinalis gave a viscous liquid with a whitish colour. The average yield of essential oil of the samples is 0.23%. This investigation allows us to support that R. officinalis includes six chemotypes in eastern Algerian. The difference of these chemotypes variants is the concentration of Eucalyptol, campene, α-pinene and camphor. The antibacterial activity of the essential oils chemotypes was evaluated against nine bacteria (Enterobacter cloacae ATCC 13047, MRSA (Methicillin-resistant Staphylococcus aureus), Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas syringae, Salmonella sp, Serratia liquefaciens ATCC 27592, Serratia marcescens ATCC 14756 and Shigella sp). The results showed that the essential oils have inhibited growth of bacterial strains. The essential oils chemotypes of Kherrata and the Bibans regions, generally exhibit antibacterial activity against the microorganisms' tested. The camphor chemotype with three variants exhibits a moderate antibacterial activity.

The analysis and identification of the essential oil components of six Rosmarinus officinalis pop... more The analysis and identification of the essential oil components of six Rosmarinus officinalis populations, from Eastern Algeria, was performed using GC-MS. The average yield of essential oil samples is 0.23%, the highest rate was observed in the essential oil of Kherrata population (0.35%), while Agmeroual population was characterised by the lowest yield (0.10%). These analyses led to the identification of 43 components. The chemical composition of the essential oil was dominated by the presence of major products, camphor (9.1-42.7%), eucalyptol (6.6-42.2%), α-pinene (11.4-25.2%), camphene (5.3-17.7%) and borneol (0.9-11.9%). Seven components were represented with average rates more than 1% in the essential oil, β-pinene, para-cymene, limonene, linalool, terpinene-4-ol, α-terpineol and β-caryophyllene. This investigation allows us to support that the species Rosmarinus officinalis of eastern Algeria includes several chemotypes. The chemotype to (eucalyptol, Camphor, α-pinene and camphene) was located in the regions of Kherrata and Bibans.