kirti mhatre - Academia.edu (original) (raw)

Papers by kirti mhatre

International Journal of Current Microbiology and Applied Sciences

Modern world can‘t be imagined without plastics due to their vast applications in contemporary li... more Modern world can‘t be imagined without plastics due to their vast applications in contemporary life. All the plastic products which are available in market are made up of Petrochemical based synthetic plastics. Such plastics are much difficult to biodegrade which results in its wise accumulation in environment and affects biogeochemical cycles enormously. However, Bioplastics made up of Polyhydroxybutyrate (PHB) provide a better alternative to life threatening petrochemical plastics. PHB is a natural biopolymer produced by numerous bacteria by using different substrates and easy to extract. Thus on nature’s demand the author was performed an experiment with the aim isolate PHB producing bacteria form natural environment and optimized the PHB producing capability of selected isolates. Among twenty two bacterial isolates, five isolates were showed prominent PHB production at 37°C in presence of glucose and sucrose as a carbon source while peptone a nitrogen source in medium with pH 7....

The textile industries discharge millions of liters of dye containing effluent into the environme... more The textile industries discharge millions of liters of dye containing effluent into the environment. The release of colored compound into water bodies is undesirable. Biodecolorization has been investigated as a method to decolorize and degrade azo dyes. In the present study, the potential Carbol fuchsin dye degrading bacteria were isolated from textile effluent samples. The most promising bacterial isolate was used for further studies. The B3 isolate showed maximum decolorization within 24 hrs. It was identified as Acinetobacter spp. And its optimum conditions for Carbol fuchsin decolorization was recorded as, PH 7, temp. 370C, Glucose as carbon source, NH4Cl and peptone as a nitrogen source. The result suggest that the isolated organism, Acinetobacter spp. can be useful tool to treat waste water containing Carbol fuchsin dye.

International Journal of Advanced Research in Science, Communication and Technology, 2022

Sea buckthorn (Hippophae rhamnoides L.) is a unique and valuable plant and has recently gained wo... more Sea buckthorn (Hippophae rhamnoides L.) is a unique and valuable plant and has recently gained worldwide attention mainly for its medicinal and nutritional potential. It is a thorny bush with yellow-orange pearl shaped fruits and has a high content of vitamins, minerals, natural antioxidants and omega-3,6 fatty acids.Doses of 2 mg/ml, 4 mg/ml and 6 mg/mlof aqueous extract of plant berry powder were evaluated against Gram positive and Gram negative microbes by usingdisc diffusion and agar well diffusion method. The zone of inhibition was compared with the standard drugs vancomycin andKanamycin(30 µg/ml). It was concluded that the aqueous extract of berry powder has antibacterial activity, which may be used to preventvarious diseases and can be incorporated in human and animal diet.

International Journal of Advanced Research in Science, Communication and Technology

Mangifera indica L. and Anacardium occidentale members of the Anacardiaceae family have gained th... more Mangifera indica L. and Anacardium occidentale members of the Anacardiaceae family have gained the attension due to their therapeutic property. The present study evaluated the presence of phytochemicals and antibacterial activity of ethanolic and methanolic extraction of both plants. Phytochemical screening showed the presence of glycosides, saponin, flavonoid, tannin, alkaloid, steroid, phenolic compound, and triterpenoid in both solvent extracts. Gram-positive and Gram-negative organisms were found to be sensitive to the ethanolic and methanolic extraction of both plants. The MIC of mango seed extract was found to be 50 μg/ml and cashew fruit extract was 8 μg/ml. The study indicates the potential future application of mango seed & Cashew fruit extract as an antibacterial agent which would be an alternative to the current chemical antibacterial agent.

Advances in Applied Science Research, 2015

Carbol fuchsin and Malachite green dyes are extensively used in textile dyeing, paper, printing a... more Carbol fuchsin and Malachite green dyes are extensively used in textile dyeing, paper, printing and other industries. Textile effluent released from industries is a complex mixture of many polluting substances including dyes and must be treated before discharged into environment because of their recalcitrant nature and potential toxicity to animals and humans. Biological treatment offers a cheaper and environment friendly alternative to dye decolorization and wastewater reutilization in industrial process. In the present studies bacteria were isolated from textile effluent and dyes decolorization assay were performed in the basal nutrient medium. The most efficient bacterial isolate was used for further optimization studies. The morphological and biochemical studies revealed the isolated organism as Enterococcus spp. The strain showed 100% and 92% decolorization of the Malachite green and Carbol fuchsin (0.02g/L) respectively within 24 h. The optimum pH and temperature for the decol...

International Journal of Advance Research, Ideas and Innovations in Technology, 2020

Release of residual dyes into natural water bodies through industrial waste water is considered t... more Release of residual dyes into natural water bodies through industrial waste water is considered to be a potential threat to the environment. There are several traditional physio-chemical treatment methods to remove such organic and inorganic dyes, but these are costly and create secondary sludge disposal problems. Bioremediation is offered the best approach to overcome the limitations of traditional methods. Thus, this study was undertaken to isolate bacteria possessing the high potential to degrade the different types of dyes. Total of 12 different dyes decolorizing bacterial isolates were obtained from textile wastewater using enrichment and isolation techniques. Out of these isolates, Citrobacter diverses was found to be the most prominent dyes decolorizing bacteria. It showed 79% to 98% decolorization of MR (Methyl red), MG (Malachite green), CR (Congo red), CV (Crystal violet), CF (Carbol fuchsin) dyes within 72 h. It showed maximum decolorization in presence of optimum growth ...

European Journal of Experimental Biology, 2015

The textile industries use different types of dyes in their processing units which are liberated ... more The textile industries use different types of dyes in their processing units which are liberated in natural water bodies through wastewater. Disposal of these dyes into the environment causes serious damage. There is the need of era to investigate new bacterial dye degraders that have potential for use in a various textile industries to treat effluents. The present study deals with the degradation of Methyl red (MR) and Carbol fuchsin (CF) by bacteria and its consortium isolated from textile effluents. Three different bacterial spp. were isolated and subjected to dye decolorization assays. The isolated bacteria were capable of decolorizing 0.002 gm/L MR and CF dye. The isolates were identified as Proteus spp., Pseudomonas spp. and Acinetobacter spp. Instead of pure bacterial isolates their consortium was exhibited the highest potential in decolorizing MR and CF up to 100% and 96%, respectively within 24 h. TheComplete degradation of dyes was confirmed by FT-IR analysis.

International Journal of Advanced Research in Science, Communication and Technology, 2022

A wide application of heavy metals in different human processes leads to its accumulation in envi... more A wide application of heavy metals in different human processes leads to its accumulation in environment. Atmospheric deposition of metals into soil ecosystem is majorly affecting the microbial count of soil and seed germination as well. In present study, soil samples were collected from metal polluted sites and agricultural fields to determine of heavy metal (Zn, Cu, Mn and Fe) content and total heterotrophic count of different soil samples. The microbial count, seed germination percentage and heavy metal concentration in agricultural field samples were found to be in permissible limit, while increased metal concentrations and lower bacterial count were observed in metal contaminated soil samples. Increasing heavy metal concentration found to be drastically affecting on total heterotrophic count of agricultural field soil samples, while high metal concentration tolerating bacterial count was found in metal contaminated soil samples. The study suggested that metal processing industr...

International Journal of Current Microbiology and Applied Sciences

Modern world can‘t be imagined without plastics due to their vast applications in contemporary li... more Modern world can‘t be imagined without plastics due to their vast applications in contemporary life. All the plastic products which are available in market are made up of Petrochemical based synthetic plastics. Such plastics are much difficult to biodegrade which results in its wise accumulation in environment and affects biogeochemical cycles enormously. However, Bioplastics made up of Polyhydroxybutyrate (PHB) provide a better alternative to life threatening petrochemical plastics. PHB is a natural biopolymer produced by numerous bacteria by using different substrates and easy to extract. Thus on nature’s demand the author was performed an experiment with the aim isolate PHB producing bacteria form natural environment and optimized the PHB producing capability of selected isolates. Among twenty two bacterial isolates, five isolates were showed prominent PHB production at 37°C in presence of glucose and sucrose as a carbon source while peptone a nitrogen source in medium with pH 7....

The textile industries discharge millions of liters of dye containing effluent into the environme... more The textile industries discharge millions of liters of dye containing effluent into the environment. The release of colored compound into water bodies is undesirable. Biodecolorization has been investigated as a method to decolorize and degrade azo dyes. In the present study, the potential Carbol fuchsin dye degrading bacteria were isolated from textile effluent samples. The most promising bacterial isolate was used for further studies. The B3 isolate showed maximum decolorization within 24 hrs. It was identified as Acinetobacter spp. And its optimum conditions for Carbol fuchsin decolorization was recorded as, PH 7, temp. 370C, Glucose as carbon source, NH4Cl and peptone as a nitrogen source. The result suggest that the isolated organism, Acinetobacter spp. can be useful tool to treat waste water containing Carbol fuchsin dye.

International Journal of Advanced Research in Science, Communication and Technology, 2022

Sea buckthorn (Hippophae rhamnoides L.) is a unique and valuable plant and has recently gained wo... more Sea buckthorn (Hippophae rhamnoides L.) is a unique and valuable plant and has recently gained worldwide attention mainly for its medicinal and nutritional potential. It is a thorny bush with yellow-orange pearl shaped fruits and has a high content of vitamins, minerals, natural antioxidants and omega-3,6 fatty acids.Doses of 2 mg/ml, 4 mg/ml and 6 mg/mlof aqueous extract of plant berry powder were evaluated against Gram positive and Gram negative microbes by usingdisc diffusion and agar well diffusion method. The zone of inhibition was compared with the standard drugs vancomycin andKanamycin(30 µg/ml). It was concluded that the aqueous extract of berry powder has antibacterial activity, which may be used to preventvarious diseases and can be incorporated in human and animal diet.

International Journal of Advanced Research in Science, Communication and Technology

Mangifera indica L. and Anacardium occidentale members of the Anacardiaceae family have gained th... more Mangifera indica L. and Anacardium occidentale members of the Anacardiaceae family have gained the attension due to their therapeutic property. The present study evaluated the presence of phytochemicals and antibacterial activity of ethanolic and methanolic extraction of both plants. Phytochemical screening showed the presence of glycosides, saponin, flavonoid, tannin, alkaloid, steroid, phenolic compound, and triterpenoid in both solvent extracts. Gram-positive and Gram-negative organisms were found to be sensitive to the ethanolic and methanolic extraction of both plants. The MIC of mango seed extract was found to be 50 μg/ml and cashew fruit extract was 8 μg/ml. The study indicates the potential future application of mango seed & Cashew fruit extract as an antibacterial agent which would be an alternative to the current chemical antibacterial agent.

Advances in Applied Science Research, 2015

Carbol fuchsin and Malachite green dyes are extensively used in textile dyeing, paper, printing a... more Carbol fuchsin and Malachite green dyes are extensively used in textile dyeing, paper, printing and other industries. Textile effluent released from industries is a complex mixture of many polluting substances including dyes and must be treated before discharged into environment because of their recalcitrant nature and potential toxicity to animals and humans. Biological treatment offers a cheaper and environment friendly alternative to dye decolorization and wastewater reutilization in industrial process. In the present studies bacteria were isolated from textile effluent and dyes decolorization assay were performed in the basal nutrient medium. The most efficient bacterial isolate was used for further optimization studies. The morphological and biochemical studies revealed the isolated organism as Enterococcus spp. The strain showed 100% and 92% decolorization of the Malachite green and Carbol fuchsin (0.02g/L) respectively within 24 h. The optimum pH and temperature for the decol...

International Journal of Advance Research, Ideas and Innovations in Technology, 2020

Release of residual dyes into natural water bodies through industrial waste water is considered t... more Release of residual dyes into natural water bodies through industrial waste water is considered to be a potential threat to the environment. There are several traditional physio-chemical treatment methods to remove such organic and inorganic dyes, but these are costly and create secondary sludge disposal problems. Bioremediation is offered the best approach to overcome the limitations of traditional methods. Thus, this study was undertaken to isolate bacteria possessing the high potential to degrade the different types of dyes. Total of 12 different dyes decolorizing bacterial isolates were obtained from textile wastewater using enrichment and isolation techniques. Out of these isolates, Citrobacter diverses was found to be the most prominent dyes decolorizing bacteria. It showed 79% to 98% decolorization of MR (Methyl red), MG (Malachite green), CR (Congo red), CV (Crystal violet), CF (Carbol fuchsin) dyes within 72 h. It showed maximum decolorization in presence of optimum growth ...

European Journal of Experimental Biology, 2015

The textile industries use different types of dyes in their processing units which are liberated ... more The textile industries use different types of dyes in their processing units which are liberated in natural water bodies through wastewater. Disposal of these dyes into the environment causes serious damage. There is the need of era to investigate new bacterial dye degraders that have potential for use in a various textile industries to treat effluents. The present study deals with the degradation of Methyl red (MR) and Carbol fuchsin (CF) by bacteria and its consortium isolated from textile effluents. Three different bacterial spp. were isolated and subjected to dye decolorization assays. The isolated bacteria were capable of decolorizing 0.002 gm/L MR and CF dye. The isolates were identified as Proteus spp., Pseudomonas spp. and Acinetobacter spp. Instead of pure bacterial isolates their consortium was exhibited the highest potential in decolorizing MR and CF up to 100% and 96%, respectively within 24 h. TheComplete degradation of dyes was confirmed by FT-IR analysis.

International Journal of Advanced Research in Science, Communication and Technology, 2022

A wide application of heavy metals in different human processes leads to its accumulation in envi... more A wide application of heavy metals in different human processes leads to its accumulation in environment. Atmospheric deposition of metals into soil ecosystem is majorly affecting the microbial count of soil and seed germination as well. In present study, soil samples were collected from metal polluted sites and agricultural fields to determine of heavy metal (Zn, Cu, Mn and Fe) content and total heterotrophic count of different soil samples. The microbial count, seed germination percentage and heavy metal concentration in agricultural field samples were found to be in permissible limit, while increased metal concentrations and lower bacterial count were observed in metal contaminated soil samples. Increasing heavy metal concentration found to be drastically affecting on total heterotrophic count of agricultural field soil samples, while high metal concentration tolerating bacterial count was found in metal contaminated soil samples. The study suggested that metal processing industr...