miaoxing wang - Academia.edu (original) (raw)

Papers by miaoxing wang

Research paper thumbnail of Src/Fas2-dependent Ephrin phosphorylation initiates Eph/Ephrin reverse signaling through Rac1 to shape columnar units in the fly brain

Columns are the morphological and functional units containing multiple neurons in the brain. The ... more Columns are the morphological and functional units containing multiple neurons in the brain. The molecular mechanisms of column formation are largely unknown. Ephrin/Eph signaling mediates a variety of developmental processes. Ephrin acts as a ligand for Eph to regulate forward signaling, while Eph acts as a ligand for Ephrin to regulate reverse signaling. However, whether and how the uni- or bi-directional Ephrin/Eph signaling is involved in column formation remains elusive. In this study, we show that Ephrin and Eph regulate the morphology and location of columnar neurons through bi-directional repulsive signaling. Furthermore, Eph ligand triggers cytoplasmic tyrosine phosphorylation of Ephrin under the control of Src kinases and Fasciclin II (Fas2), forming Ephrin/Src/Fas2 complex to promote reverse signaling through a downstream regulator, Rac1. This study presents for the first time a unified picture of the molecular interactions in the defined context of column formation using...

Research paper thumbnail of Quantitative analysis of the roles of IRM cell adhesion molecules in column formation in the fly brain

Development, Growth & Differentiation

Research paper thumbnail of DWnt4 and DWnt10 Regulate Morphogenesis and Arrangement of Columnar Units via Fz2/PCP Signaling in the Drosophila Brain

Cell Reports, 2020

Highlights d Neurons assemble to establish a column d Planar cell polarity regulates the orientat... more Highlights d Neurons assemble to establish a column d Planar cell polarity regulates the orientation of neurons and columns d Wnt ligands globally regulate neuronal orientation and column arrangement

Research paper thumbnail of Correction to: A continuation method for spatially discretized models with nonlocal interactions conserving size and shape of cells and lattices

Journal of Mathematical Biology, 2021

Research paper thumbnail of A continuation method for spatially discretized models with nonlocal interactions conserving size and shape of cells and lattices

Journal of Mathematical Biology, 2020

In this paper, we introduce a continuation method for the spatially discretized models, while con... more In this paper, we introduce a continuation method for the spatially discretized models, while conserving the size and shape of the cells and lattices. This proposed method is realized using the shift operators and nonlocal operators of convolution types. Through this method and using the shift operator, the nonlinear spatially discretized model on the uniform and nonuniform lattices can be systematically converted into a spatially continuous model; this renders both models point-wisely equivalent. Moreover, by the convolution with suitable kernels, we mollify the shift operator and approximate the spatially discretized models using the nonlocal evolution equations, rendering suitable for the application in both experimental and mathematical analyses. We also demonstrate that this approximation is supported by the singular limit analysis, and that the information of the lattice and cells is expressed in the shift and nonlocal operators. The continuous models designed using our method...

Research paper thumbnail of Dscam1 establishes the columnar units through lineage-dependent repulsion between sister neurons in the fly brain

Nature Communications, 2020

The brain is organized morphologically and functionally into a columnar structure. According to t... more The brain is organized morphologically and functionally into a columnar structure. According to the radial unit hypothesis, neurons from the same lineage form a radial unit that contributes to column formation. However, the molecular mechanisms that link neuronal lineage and column formation remain elusive. Here, we show that neurons from the same lineage project to different columns under control of Down syndrome cell adhesion molecule (Dscam) in the fly brain. Dscam1 is temporally expressed in newly born neuroblasts and is inherited by their daughter neurons. The transient transcription of Dscam1 in neuroblasts enables the expression of the same Dscam1 splice isoform within cells of the same lineage, causing lineage-dependent repulsion. In the absence of Dscam1 function, neurons from the same lineage project to the same column. When the splice diversity of Dscam1 is reduced, column formation is significantly compromised. Thus, Dscam1 controls column formation through lineage-depen...

Research paper thumbnail of Intracellular trafficking of Notch orchestrates temporal dynamics of Notch activity in the fly brain

Nature Communications, 2021

While Delta non-autonomously activates Notch in neighboring cells, it autonomously inactivates No... more While Delta non-autonomously activates Notch in neighboring cells, it autonomously inactivates Notch throughcis-inhibition, the molecular mechanism and biological roles of which remain elusive. The wave of differentiation in theDrosophilabrain, the ‘proneural wave’, is an excellent model for studying Notch signaling in vivo. Here, we show that strong nonlinearity incis-inhibition reproduces the second peak of Notch activity behind the proneural wave in silico. Based on this, we demonstrate that Delta expression induces a quick degradation of Notch in late endosomes and the formation of the twin peaks of Notch activity in vivo. Indeed, the amount of Notch is upregulated and the twin peaks are fused forming a single peak when the function of Delta or late endosomes is compromised. Additionally, we show that the second Notch peak behind the wavefront controls neurogenesis. Thus, intracellular trafficking of Notch orchestrates the temporal dynamics of Notch activity and the temporal pat...

Research paper thumbnail of Src/Fas2-dependent Ephrin phosphorylation initiates Eph/Ephrin reverse signaling through Rac1 to shape columnar units in the fly brain

Columns are the morphological and functional units containing multiple neurons in the brain. The ... more Columns are the morphological and functional units containing multiple neurons in the brain. The molecular mechanisms of column formation are largely unknown. Ephrin/Eph signaling mediates a variety of developmental processes. Ephrin acts as a ligand for Eph to regulate forward signaling, while Eph acts as a ligand for Ephrin to regulate reverse signaling. However, whether and how the uni- or bi-directional Ephrin/Eph signaling is involved in column formation remains elusive. In this study, we show that Ephrin and Eph regulate the morphology and location of columnar neurons through bi-directional repulsive signaling. Furthermore, Eph ligand triggers cytoplasmic tyrosine phosphorylation of Ephrin under the control of Src kinases and Fasciclin II (Fas2), forming Ephrin/Src/Fas2 complex to promote reverse signaling through a downstream regulator, Rac1. This study presents for the first time a unified picture of the molecular interactions in the defined context of column formation using...

Research paper thumbnail of Quantitative analysis of the roles of IRM cell adhesion molecules in column formation in the fly brain

Development, Growth & Differentiation

Research paper thumbnail of DWnt4 and DWnt10 Regulate Morphogenesis and Arrangement of Columnar Units via Fz2/PCP Signaling in the Drosophila Brain

Cell Reports, 2020

Highlights d Neurons assemble to establish a column d Planar cell polarity regulates the orientat... more Highlights d Neurons assemble to establish a column d Planar cell polarity regulates the orientation of neurons and columns d Wnt ligands globally regulate neuronal orientation and column arrangement

Research paper thumbnail of Correction to: A continuation method for spatially discretized models with nonlocal interactions conserving size and shape of cells and lattices

Journal of Mathematical Biology, 2021

Research paper thumbnail of A continuation method for spatially discretized models with nonlocal interactions conserving size and shape of cells and lattices

Journal of Mathematical Biology, 2020

In this paper, we introduce a continuation method for the spatially discretized models, while con... more In this paper, we introduce a continuation method for the spatially discretized models, while conserving the size and shape of the cells and lattices. This proposed method is realized using the shift operators and nonlocal operators of convolution types. Through this method and using the shift operator, the nonlinear spatially discretized model on the uniform and nonuniform lattices can be systematically converted into a spatially continuous model; this renders both models point-wisely equivalent. Moreover, by the convolution with suitable kernels, we mollify the shift operator and approximate the spatially discretized models using the nonlocal evolution equations, rendering suitable for the application in both experimental and mathematical analyses. We also demonstrate that this approximation is supported by the singular limit analysis, and that the information of the lattice and cells is expressed in the shift and nonlocal operators. The continuous models designed using our method...

Research paper thumbnail of Dscam1 establishes the columnar units through lineage-dependent repulsion between sister neurons in the fly brain

Nature Communications, 2020

The brain is organized morphologically and functionally into a columnar structure. According to t... more The brain is organized morphologically and functionally into a columnar structure. According to the radial unit hypothesis, neurons from the same lineage form a radial unit that contributes to column formation. However, the molecular mechanisms that link neuronal lineage and column formation remain elusive. Here, we show that neurons from the same lineage project to different columns under control of Down syndrome cell adhesion molecule (Dscam) in the fly brain. Dscam1 is temporally expressed in newly born neuroblasts and is inherited by their daughter neurons. The transient transcription of Dscam1 in neuroblasts enables the expression of the same Dscam1 splice isoform within cells of the same lineage, causing lineage-dependent repulsion. In the absence of Dscam1 function, neurons from the same lineage project to the same column. When the splice diversity of Dscam1 is reduced, column formation is significantly compromised. Thus, Dscam1 controls column formation through lineage-depen...

Research paper thumbnail of Intracellular trafficking of Notch orchestrates temporal dynamics of Notch activity in the fly brain

Nature Communications, 2021

While Delta non-autonomously activates Notch in neighboring cells, it autonomously inactivates No... more While Delta non-autonomously activates Notch in neighboring cells, it autonomously inactivates Notch throughcis-inhibition, the molecular mechanism and biological roles of which remain elusive. The wave of differentiation in theDrosophilabrain, the ‘proneural wave’, is an excellent model for studying Notch signaling in vivo. Here, we show that strong nonlinearity incis-inhibition reproduces the second peak of Notch activity behind the proneural wave in silico. Based on this, we demonstrate that Delta expression induces a quick degradation of Notch in late endosomes and the formation of the twin peaks of Notch activity in vivo. Indeed, the amount of Notch is upregulated and the twin peaks are fused forming a single peak when the function of Delta or late endosomes is compromised. Additionally, we show that the second Notch peak behind the wavefront controls neurogenesis. Thus, intracellular trafficking of Notch orchestrates the temporal dynamics of Notch activity and the temporal pat...