giorgi mikadze - Academia.edu (original) (raw)
Papers by giorgi mikadze
Lecture notes in networks and systems, 2024
The paper deals with the new technological processes of obtaining vital microelements, so-called ... more The paper deals with the new technological processes of obtaining vital microelements, so-called biometals, based on the possibility of relevant oxide conversion in gaseous atmosphere with ultra-low oxidation potential. Specic ways are shown for achieving ultra-deep rarefaction of atmosphere of oxygen (Po2≈10-26 atm). Temperature range of the current processes and the ratio of initial reagents are dened, which determine the qualitative and quantitative sides of the condensates extracted in the reactor.
EFSA Supporting Publications, 2021
Neoplasia, 2020
Hypoxia is frequently observed in human prostate cancer, and is associated with chemoresistance, ... more Hypoxia is frequently observed in human prostate cancer, and is associated with chemoresistance, radioresistance, metastasis, and castrate-resistance. Our purpose in these studies was to perform hypoxia theranostics by combining in vivo hypoxia imaging and hypoxic cancer cell targeting in a human prostate cancer xenograft. This was achieved by engineering PC3 human prostate cancer cells to express luciferase as well as a prodrug enzyme, yeast cytosine deaminase, under control of hypoxic response elements (HREs). Cancer cells display an adaptive response to hypoxia through the activation of several genes mediated by the binding of hypoxia inducible factors (HIFs) to HRE in the promoter region of target gene that results in their increased transcription. HIFs promote key steps in tumorigenesis, including angiogenesis, metabolism, proliferation, metastasis, and differentiation. HRE-driven luciferase expression allowed us to detect hypoxia in vivo to time the administration of the nontoxic prodrug 5-fluorocytosine that was converted by yeast cytosine deaminase, expressed under HRE regulation, to the chemotherapy agent 5-fluorouracil to target hypoxic cells. Conversion of 5-fluorocytosine to 5-fluorouracil was detected in vivo by 19 F magnetic resonance spectroscopy. Morphological and immunohistochemical staining and molecular analyses were performed to characterize tumor microenvironment changes in cancerassociated fibroblasts, cell viability, collagen 1 fiber patterns, and HIF-1 α. These studies expand our understanding of the effects of eliminating hypoxic cancer cells on the tumor microenvironment and in reducing stromal cell populations such as cancer-associated fibroblasts.
The paper deals with the research of air oxidation kinetics of silver-based composite. Oxidation ... more The paper deals with the research of air oxidation kinetics of silver-based composite. Oxidation kinetics of silver-based composite material, containing 10 weight percent of nickel, was studied. Changes of specific weight gain experimental curves in air in the 500-900oС temperature range are well described by the law of complex parabola. On the basis of this equation calculation formulas are obtained allowing constructing theoretical oxidation curves of the studied objects, which practically coincide with the experimental results. The temperature dependence of linear and parabolic constants of oxidation process is defined.
Lecture notes in networks and systems, 2024
The paper deals with the new technological processes of obtaining vital microelements, so-called ... more The paper deals with the new technological processes of obtaining vital microelements, so-called biometals, based on the possibility of relevant oxide conversion in gaseous atmosphere with ultra-low oxidation potential. Specic ways are shown for achieving ultra-deep rarefaction of atmosphere of oxygen (Po2≈10-26 atm). Temperature range of the current processes and the ratio of initial reagents are dened, which determine the qualitative and quantitative sides of the condensates extracted in the reactor.
EFSA Supporting Publications, 2021
Neoplasia, 2020
Hypoxia is frequently observed in human prostate cancer, and is associated with chemoresistance, ... more Hypoxia is frequently observed in human prostate cancer, and is associated with chemoresistance, radioresistance, metastasis, and castrate-resistance. Our purpose in these studies was to perform hypoxia theranostics by combining in vivo hypoxia imaging and hypoxic cancer cell targeting in a human prostate cancer xenograft. This was achieved by engineering PC3 human prostate cancer cells to express luciferase as well as a prodrug enzyme, yeast cytosine deaminase, under control of hypoxic response elements (HREs). Cancer cells display an adaptive response to hypoxia through the activation of several genes mediated by the binding of hypoxia inducible factors (HIFs) to HRE in the promoter region of target gene that results in their increased transcription. HIFs promote key steps in tumorigenesis, including angiogenesis, metabolism, proliferation, metastasis, and differentiation. HRE-driven luciferase expression allowed us to detect hypoxia in vivo to time the administration of the nontoxic prodrug 5-fluorocytosine that was converted by yeast cytosine deaminase, expressed under HRE regulation, to the chemotherapy agent 5-fluorouracil to target hypoxic cells. Conversion of 5-fluorocytosine to 5-fluorouracil was detected in vivo by 19 F magnetic resonance spectroscopy. Morphological and immunohistochemical staining and molecular analyses were performed to characterize tumor microenvironment changes in cancerassociated fibroblasts, cell viability, collagen 1 fiber patterns, and HIF-1 α. These studies expand our understanding of the effects of eliminating hypoxic cancer cells on the tumor microenvironment and in reducing stromal cell populations such as cancer-associated fibroblasts.
The paper deals with the research of air oxidation kinetics of silver-based composite. Oxidation ... more The paper deals with the research of air oxidation kinetics of silver-based composite. Oxidation kinetics of silver-based composite material, containing 10 weight percent of nickel, was studied. Changes of specific weight gain experimental curves in air in the 500-900oС temperature range are well described by the law of complex parabola. On the basis of this equation calculation formulas are obtained allowing constructing theoretical oxidation curves of the studied objects, which practically coincide with the experimental results. The temperature dependence of linear and parabolic constants of oxidation process is defined.