milos pavlovic - Academia.edu (original) (raw)

Papers by milos pavlovic

Research paper thumbnail of Lactobionic acid in a natural alkylpolyglucoside-based vehicle: assessing safety and efficacy aspects in comparison to glycolic acid

Journal of Cosmetic Dermatology, 2010

Background/aims Lactobionic acid (LA) is a newer cosmeceutical active belonging to the class of ... more Background/aims Lactobionic acid (LA) is a newer cosmeceutical active belonging to the class of alpha-hydroxyacids (AHAs), showing advantages over them. The aim of part I of this study was to compare efficacy and irritation potential of LA vs. glycolic acid (GA) from two types of vehicles – gel and emulsion. In part II, effects of LA-containing emulsions based on a new, natural emulsifier of alkylpolyglucoside (APG) type were evaluated.Methods Skin bioengineering was used on 77 healthy volunteers to assess: color as erythema and melanin (MI) index, transepidermal water loss, electrical capacitance and pH of the skin. In part I of the study, the parameters were measured after occlusion and periodically during 2 weeks of test samples application; in part II parameters were measured periodically during 4 weeks.Results/conclusion LA-containing samples has produced better skin performance when compared with corresponding GA-containing ones, particularly the lack of both skin irritation and skin barrier impairment. When used in vehicles based on a new APG-emulsifier, LA and GA have shown better efficacy, emphasizing the importance of vehicle on the effects of topical actives. LA (6%) in the emulsion based on APGs could be proposed as an alternative to low-molecular AHAs in cosmeceuticals.

Research paper thumbnail of Emulation of gain measurements of standard gain pyramidal horns using 3D EM solver

Pyramidal horn antennas are mostly used as “known gain” antennas in measurements of antennas with... more Pyramidal horn antennas are mostly used as “known gain” antennas in measurements of antennas with gain to be determined. Manufacturers of antennas, institutions for standardization and measurement laboratories offer tabulated data for antenna gain to be used in measurements with defined accuracy (typically 0.5 dB, 0.25 dB). There has been continuous effort to achieve a better accuracy in measuring antenna gain. The ultimate goal is to have accuracy of 0.1 dB for SGH. In this paper two most commonly measurement techniques are efficiently emulated using commercial full wave EM solver in order to explore possible causes for measurement errors. It was shown that, when ideally applied, these two measurement techniques give almost exactly the same results for gain as direct full wave solution.

Research paper thumbnail of Optimum choice of currents' expansion order in MLFMM algorithm for electromagnetic scattering

The goal of this paper is to investigate how accuracy and efficiency of MLFMM of a given level de... more The goal of this paper is to investigate how accuracy and efficiency of MLFMM of a given level depends on the order of approximation. We performed this investigation using a corner scatterer of side 20 lambda as a benchmark. We chose this model because its RCS results show several distinctive lobes in specific directions and at different levels, calculation of

Research paper thumbnail of Full wave analysis of periodic and random surface distortions on reflector antennas

The focus of this paper is to present full wave analysis of periodic and random surface distortio... more The focus of this paper is to present full wave analysis of periodic and random surface distortions on reflector antennas. Three models of distortions are used - random distortion, one dimensional cosine and two dimension cosine distortion. Also, in order to compare results, reflector with ideally flat surface was used as referent model. In this paper, the distorted reflector surface points are approximated by summing two components: an undistorted (ideal) surface component and a surface error component. For each case, radiation patterns of the reflector are simulated and influence to main lobe and grating lobe is observed for reflector antennas up to 200 wavelengths in diameter by using an efficient MoM 3D solver.

Research paper thumbnail of Role of β2 Integrins in the Binding of Thymocytes to Rat Thymic Macrophages

Clinical & Developmental Immunology, 1994

A role of 32 integrins and one of their ligands, ICAM-1, in thymic macrophage (TMF)/thymocyte int... more A role of 32 integrins and one of their ligands, ICAM-1, in thymic macrophage (TMF)/thymocyte interactions was studied. TMF were isolated as adherent cells from 4-day old culture of thymic-cell suspensions either from normal or hydrocortisone-treated rats. Adherent cells were 94-98% positive with ED1 (a pan-macrophage marker). The majority of them (75-95%) expressed the CD11b and CD18 molecules, and 60-70% expressed CD54 (ICAM-1). A low proportion of TMF (10-20%) expressed CDlla (LFA-1). The expression of all these antigens was upregulated by IFN-3 and TNF-a. The effect of these mAbs on TMF/thymocyte binding was studied using a simple rosette assay by incubating unstimulated or IFN-3 or TNF-a stimulated TMF, grown on microscopic slides with resting or ConA +IL-2 activated thymocytes. It was found that LFA-1/CD18 and ICAM-1 play a significant role in the TMF/thymocyte adhesion. In addition, a LFA-l-dependent/ICAM-1-independent adhesion pathway was observed, suggesting that LFA-1 might use another ligand. The inhibitory effect of anti-CD18 mAb (WT-3) was higher than the effect of anti-LFA-1 mAb (WT-1) and was a consequence of blocking the CD18 chain both on thymocytes and TMF. No significant difference in the expression and function of adhesion molecules was found between TMF obtained from normal or hydrocortisone-treated rats. The involvement of CD1 lb in these processes was of lesser importance than the role of the CD11a molecule. By using mAbs to different epitopes of the CD11b molecule, such as OX-42 (anti-CD11b/CD11c), ED7, and ED8 (anti-CD11b), it was found that they were either slightly or moderately inhibitory under certain experimental conditions or did not significantly modulate TMF/thymocyte binding. OX-42 was slightly stimulatory in some experiments. Cumulatively, these results show that 2 integrins play a significant role in TMF/thymocyte interactions and probably contribute to T-cell development in vivo.

Research paper thumbnail of Lactobionic acid in a natural alkylpolyglucoside-based vehicle: assessing safety and efficacy aspects in comparison to glycolic acid

Journal of Cosmetic Dermatology, 2010

Background/aims Lactobionic acid (LA) is a newer cosmeceutical active belonging to the class of ... more Background/aims Lactobionic acid (LA) is a newer cosmeceutical active belonging to the class of alpha-hydroxyacids (AHAs), showing advantages over them. The aim of part I of this study was to compare efficacy and irritation potential of LA vs. glycolic acid (GA) from two types of vehicles – gel and emulsion. In part II, effects of LA-containing emulsions based on a new, natural emulsifier of alkylpolyglucoside (APG) type were evaluated.Methods Skin bioengineering was used on 77 healthy volunteers to assess: color as erythema and melanin (MI) index, transepidermal water loss, electrical capacitance and pH of the skin. In part I of the study, the parameters were measured after occlusion and periodically during 2 weeks of test samples application; in part II parameters were measured periodically during 4 weeks.Results/conclusion LA-containing samples has produced better skin performance when compared with corresponding GA-containing ones, particularly the lack of both skin irritation and skin barrier impairment. When used in vehicles based on a new APG-emulsifier, LA and GA have shown better efficacy, emphasizing the importance of vehicle on the effects of topical actives. LA (6%) in the emulsion based on APGs could be proposed as an alternative to low-molecular AHAs in cosmeceuticals.

Research paper thumbnail of Emulation of gain measurements of standard gain pyramidal horns using 3D EM solver

Pyramidal horn antennas are mostly used as “known gain” antennas in measurements of antennas with... more Pyramidal horn antennas are mostly used as “known gain” antennas in measurements of antennas with gain to be determined. Manufacturers of antennas, institutions for standardization and measurement laboratories offer tabulated data for antenna gain to be used in measurements with defined accuracy (typically 0.5 dB, 0.25 dB). There has been continuous effort to achieve a better accuracy in measuring antenna gain. The ultimate goal is to have accuracy of 0.1 dB for SGH. In this paper two most commonly measurement techniques are efficiently emulated using commercial full wave EM solver in order to explore possible causes for measurement errors. It was shown that, when ideally applied, these two measurement techniques give almost exactly the same results for gain as direct full wave solution.

Research paper thumbnail of Optimum choice of currents' expansion order in MLFMM algorithm for electromagnetic scattering

The goal of this paper is to investigate how accuracy and efficiency of MLFMM of a given level de... more The goal of this paper is to investigate how accuracy and efficiency of MLFMM of a given level depends on the order of approximation. We performed this investigation using a corner scatterer of side 20 lambda as a benchmark. We chose this model because its RCS results show several distinctive lobes in specific directions and at different levels, calculation of

Research paper thumbnail of Full wave analysis of periodic and random surface distortions on reflector antennas

The focus of this paper is to present full wave analysis of periodic and random surface distortio... more The focus of this paper is to present full wave analysis of periodic and random surface distortions on reflector antennas. Three models of distortions are used - random distortion, one dimensional cosine and two dimension cosine distortion. Also, in order to compare results, reflector with ideally flat surface was used as referent model. In this paper, the distorted reflector surface points are approximated by summing two components: an undistorted (ideal) surface component and a surface error component. For each case, radiation patterns of the reflector are simulated and influence to main lobe and grating lobe is observed for reflector antennas up to 200 wavelengths in diameter by using an efficient MoM 3D solver.

Research paper thumbnail of Role of β2 Integrins in the Binding of Thymocytes to Rat Thymic Macrophages

Clinical & Developmental Immunology, 1994

A role of 32 integrins and one of their ligands, ICAM-1, in thymic macrophage (TMF)/thymocyte int... more A role of 32 integrins and one of their ligands, ICAM-1, in thymic macrophage (TMF)/thymocyte interactions was studied. TMF were isolated as adherent cells from 4-day old culture of thymic-cell suspensions either from normal or hydrocortisone-treated rats. Adherent cells were 94-98% positive with ED1 (a pan-macrophage marker). The majority of them (75-95%) expressed the CD11b and CD18 molecules, and 60-70% expressed CD54 (ICAM-1). A low proportion of TMF (10-20%) expressed CDlla (LFA-1). The expression of all these antigens was upregulated by IFN-3 and TNF-a. The effect of these mAbs on TMF/thymocyte binding was studied using a simple rosette assay by incubating unstimulated or IFN-3 or TNF-a stimulated TMF, grown on microscopic slides with resting or ConA +IL-2 activated thymocytes. It was found that LFA-1/CD18 and ICAM-1 play a significant role in the TMF/thymocyte adhesion. In addition, a LFA-l-dependent/ICAM-1-independent adhesion pathway was observed, suggesting that LFA-1 might use another ligand. The inhibitory effect of anti-CD18 mAb (WT-3) was higher than the effect of anti-LFA-1 mAb (WT-1) and was a consequence of blocking the CD18 chain both on thymocytes and TMF. No significant difference in the expression and function of adhesion molecules was found between TMF obtained from normal or hydrocortisone-treated rats. The involvement of CD1 lb in these processes was of lesser importance than the role of the CD11a molecule. By using mAbs to different epitopes of the CD11b molecule, such as OX-42 (anti-CD11b/CD11c), ED7, and ED8 (anti-CD11b), it was found that they were either slightly or moderately inhibitory under certain experimental conditions or did not significantly modulate TMF/thymocyte binding. OX-42 was slightly stimulatory in some experiments. Cumulatively, these results show that 2 integrins play a significant role in TMF/thymocyte interactions and probably contribute to T-cell development in vivo.