moanaro lemtur - Academia.edu (original) (raw)
Papers by moanaro lemtur
South African Journal of Botany
The peach fruit fly, <i>Bactrocera zonata</i>, is among the most serious and polyphag... more The peach fruit fly, <i>Bactrocera zonata</i>, is among the most serious and polyphagous insect pest of fruit crops in many parts of the world under genus <i>Bactrocera</i>. In the present study, the genetic structure, diversity and demographic history of <i>B. zonata</i> in India were inferred from mitochondrial <i>cytochrome oxidase 1</i> (<i>cox1</i>) and <i>NADH dehydrogenase 1</i> (<i>nad1</i>) sequences. The efficiency of DNA barcodes for identification of <i>B. zonata</i> was also tested. Genetic diversity indices [number of haplotypes (<i>H</i>), haplotype diversity (<i>Hd</i>), nucleotide diversity (<i>π</i>) and average number of nucleotide differences (<i>k</i>)] of <i>B. zonata</i> populations across India maintain high level of genetic diversity without isolation by distance among the geographic regions. Non-signific...
An experiment was conducted at ICAR RCER, RC, Ranchi, India during 2012-13 to assess the potentia... more An experiment was conducted at ICAR RCER, RC, Ranchi, India during 2012-13 to assess the potential allelopathic effect of leaf extract of eleven tree species [Mango (Mangifera indica), Poplar (Populus deltoides),Arjun (Terminalia arjuna), Palas (Butea monosperma), Bakaine (Melia azedarach), Acacia mangium, Acacia auriculiformis, Bael (Aegle marmelos), Teak (Tectona grandis), Jackfruit (Artocarpus heterophyllus), Shisham (Dalbergia sissoo)] on nine annual crops (black gram, gram, pea, sponge gourd, mustard, okra, brinjal, chilli, tomato). Data were recorded on seed germination (after 10 days of seed sowing) and growth and biochemical composition of 20 days old seedlings. Reduction in germination % of annual crops was recorded with application of leaf leachate of each tree species except D. sissoo, A. heterophyllus, A. marmelos and A. mangium. With respect to weight of whole plant, application of leaf extract of M. indica, B. monosperma, A. mangium, A. auriculiformis and A. marmelos r...
Mitochondrial DNA Part B, 2018
The mango leafhopper, Amritodus atkinsoni is a serious and an endemic insect pest of mango in Ind... more The mango leafhopper, Amritodus atkinsoni is a serious and an endemic insect pest of mango in India. We analyzed mtCOI gene sequences from six Indian populations of A. atkinsoni for genetic diversity and population structure. The analysis of mtCOI sequence revealed 14 unique haplotypes and a low level of nucleotide diversity. mtCOI gene sequence analysis also revealed that A. atkinsoni specimens were clearly differentiated from other closely related species with a high level of accuracy.
Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis, Jul 15, 2017
The peach fruit fly, Bactrocera zonata, is among the most serious and polyphagous insect pest of ... more The peach fruit fly, Bactrocera zonata, is among the most serious and polyphagous insect pest of fruit crops in many parts of the world under genus Bactrocera. In the present study, the genetic structure, diversity and demographic history of B. zonata in India were inferred from mitochondrial cytochrome oxidase 1 (cox1) and NADH dehydrogenase 1 (nad1) sequences. The efficiency of DNA barcodes for identification of B. zonata was also tested. Genetic diversity indices [number of haplotypes (H), haplotype diversity (Hd), nucleotide diversity (π) and average number of nucleotide differences (k)] of B. zonata populations across India maintain high level of genetic diversity without isolation by distance among the geographic regions. Non-significant negative correlation between pairwise Fst and geographic distance suggests a high level of gene flow among studied populations of B. zonata. The possibility of sudden expansion of B. zonata revealed through mismatch distribution analysis as we...
Energy Sources, Part A: Recovery, Utilization, and Environmental Effects, 2016
Quantitative analysis of 24 indigenous firewood tree/shrub species of Eastern Himalayan was carri... more Quantitative analysis of 24 indigenous firewood tree/shrub species of Eastern Himalayan was carried out to identify those species with potential for firewood production. A fuelwood value index (FVI) was the criterion for screening of the species and is defined as the calorific value X density/ash content. The results showed that Camellia kissi Wall., Styrax serrulatum Roxb., Litsea lancifolia Roxb., Leea indica (Burm. F) Merr, and Maesa indica (Roxb.) Wall are suitable fuelwood species.
Genetica, 2016
The study examined the genetic diversity and demographic history of Bactrocera dorsalis, a destru... more The study examined the genetic diversity and demographic history of Bactrocera dorsalis, a destructive and polyphagous insect pest of fruit crops in diverse geographic regions of India. 19 widely dispersed populations of the fly from India and other Asian countries were analysed using partial sequences of mitochondrial cytochrome oxidase I (cox1) and NADH dehydrogenase 1 (nad1) genes to investigate genetic diversity, genetic structure, and demographic history in the region. Genetic diversity indices [number of haplotypes (H), haloptype diversity (Hd), nucleotide diversity (p) and average number of nucleotide difference (k)] of populations revealed that B. dorsalis maintains fairly high level of genetic diversity without isolation by distance among the geographic regions. Demographic analysis showed significant (negative) Tajimas' D and Fu's F S with non significant sum of squared deviations (SSD) values, which indicate the possibility of recent sudden expansion of species and is further supported through distinctively star-like distribution structure of haplotypes among populations. Thus, the results indicate that both ongoing and historical factors have played important role in determining the genetic structure and diversity of the species in India. Consequently, sterile insect technique (SIT) could be a possible management strategy of species in the regions.
Phytoparasitica, 2016
The aim of the present study was to identify the cultivable gut bacteria associated with peach fr... more The aim of the present study was to identify the cultivable gut bacteria associated with peach fruit fly, Bactrocera zonata, and evaluate their potential to attract adults of B. zonata. Based on culture-dependent characterization methods and 16S rRNA gene sequence analysis, bacteria were identified as members of family Enterobacteriaceae (BZM1, Klebsiella oxytoca), Microbacteriacea (BZM2, Microbacterium spp.) and Nocardiaceae (BZM4, Rhodococcus spp.). Molecular phylogeny placed Klebsiella oxytoca within gram negative γ-proteobacteria whereas, Microbacterium spp. and Rhodococcus spp. were clustered under gram positive Actinobacteria group in family Microbacteriacea and Nocardiaceae, respectively. 16S rRNA gene sequence comparison with the available NCBI database sequences further confirmed the characterizations of bacterial symbionts. Population of these bacterial species increased significantly up to the 11 th day after emergence of adults and thereafter it remains constant. Among 3 bacterial symbionts, metabolites produced from K. oxytoca had the highest attraction to the B. zonata adult females over metabolites produced from other bacteria and their combinations in field bioassay. The B. zonata adult male flies attracted to metabolites produced from each bacterial symbionts alone and their combinations were less in number with comparison to the B. zonata adult females. The present study provides the first description of the attractancy potential of metabolites produced by gut microbial community of B. zonata in open field condition. This study results may prompt the development of a female-targeted population control strategy for this fly.
The study examined the genetic diversity and demographic history of Bactrocera dorsalis, a destru... more The study examined the genetic diversity and demographic history of Bactrocera dorsalis, a destructive and polyphagous insect pest of fruit crops in diverse geographic regions of India. 19 widely dispersed populations of the fly from India and other Asian countries were analysed using partial sequences of mitochondrial cytochrome oxidase I (cox1) and NADH dehydrogenase 1 (nad1) genes to investigate genetic diversity, genetic structure, and demographic history in the region. Genetic diversity indices [number of haplotypes (H), haloptype diversity (Hd), nucleotide diversity (π) and average number of nucleotide difference (k)] of populations revealed that B. dorsalis maintains fairly high level of genetic diversity without isolation by distance among the geographic regions. Demographic analysis showed significant (negative) Tajimas' D and Fu's F S with non significant sum of squared deviations (SSD) values, which indicate the possibility of recent sudden expansion of species and is further supported through distinctively star-like distribution structure of haplotypes among populations. Thus, the results indicate that both ongoing and historical factors have played important role in determining the genetic structure and diversity of the species in India. Consequently, sterile insect technique (SIT) could be a possible management strategy of species in the regions.
South African Journal of Botany
The peach fruit fly, <i>Bactrocera zonata</i>, is among the most serious and polyphag... more The peach fruit fly, <i>Bactrocera zonata</i>, is among the most serious and polyphagous insect pest of fruit crops in many parts of the world under genus <i>Bactrocera</i>. In the present study, the genetic structure, diversity and demographic history of <i>B. zonata</i> in India were inferred from mitochondrial <i>cytochrome oxidase 1</i> (<i>cox1</i>) and <i>NADH dehydrogenase 1</i> (<i>nad1</i>) sequences. The efficiency of DNA barcodes for identification of <i>B. zonata</i> was also tested. Genetic diversity indices [number of haplotypes (<i>H</i>), haplotype diversity (<i>Hd</i>), nucleotide diversity (<i>π</i>) and average number of nucleotide differences (<i>k</i>)] of <i>B. zonata</i> populations across India maintain high level of genetic diversity without isolation by distance among the geographic regions. Non-signific...
An experiment was conducted at ICAR RCER, RC, Ranchi, India during 2012-13 to assess the potentia... more An experiment was conducted at ICAR RCER, RC, Ranchi, India during 2012-13 to assess the potential allelopathic effect of leaf extract of eleven tree species [Mango (Mangifera indica), Poplar (Populus deltoides),Arjun (Terminalia arjuna), Palas (Butea monosperma), Bakaine (Melia azedarach), Acacia mangium, Acacia auriculiformis, Bael (Aegle marmelos), Teak (Tectona grandis), Jackfruit (Artocarpus heterophyllus), Shisham (Dalbergia sissoo)] on nine annual crops (black gram, gram, pea, sponge gourd, mustard, okra, brinjal, chilli, tomato). Data were recorded on seed germination (after 10 days of seed sowing) and growth and biochemical composition of 20 days old seedlings. Reduction in germination % of annual crops was recorded with application of leaf leachate of each tree species except D. sissoo, A. heterophyllus, A. marmelos and A. mangium. With respect to weight of whole plant, application of leaf extract of M. indica, B. monosperma, A. mangium, A. auriculiformis and A. marmelos r...
Mitochondrial DNA Part B, 2018
The mango leafhopper, Amritodus atkinsoni is a serious and an endemic insect pest of mango in Ind... more The mango leafhopper, Amritodus atkinsoni is a serious and an endemic insect pest of mango in India. We analyzed mtCOI gene sequences from six Indian populations of A. atkinsoni for genetic diversity and population structure. The analysis of mtCOI sequence revealed 14 unique haplotypes and a low level of nucleotide diversity. mtCOI gene sequence analysis also revealed that A. atkinsoni specimens were clearly differentiated from other closely related species with a high level of accuracy.
Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis, Jul 15, 2017
The peach fruit fly, Bactrocera zonata, is among the most serious and polyphagous insect pest of ... more The peach fruit fly, Bactrocera zonata, is among the most serious and polyphagous insect pest of fruit crops in many parts of the world under genus Bactrocera. In the present study, the genetic structure, diversity and demographic history of B. zonata in India were inferred from mitochondrial cytochrome oxidase 1 (cox1) and NADH dehydrogenase 1 (nad1) sequences. The efficiency of DNA barcodes for identification of B. zonata was also tested. Genetic diversity indices [number of haplotypes (H), haplotype diversity (Hd), nucleotide diversity (π) and average number of nucleotide differences (k)] of B. zonata populations across India maintain high level of genetic diversity without isolation by distance among the geographic regions. Non-significant negative correlation between pairwise Fst and geographic distance suggests a high level of gene flow among studied populations of B. zonata. The possibility of sudden expansion of B. zonata revealed through mismatch distribution analysis as we...
Energy Sources, Part A: Recovery, Utilization, and Environmental Effects, 2016
Quantitative analysis of 24 indigenous firewood tree/shrub species of Eastern Himalayan was carri... more Quantitative analysis of 24 indigenous firewood tree/shrub species of Eastern Himalayan was carried out to identify those species with potential for firewood production. A fuelwood value index (FVI) was the criterion for screening of the species and is defined as the calorific value X density/ash content. The results showed that Camellia kissi Wall., Styrax serrulatum Roxb., Litsea lancifolia Roxb., Leea indica (Burm. F) Merr, and Maesa indica (Roxb.) Wall are suitable fuelwood species.
Genetica, 2016
The study examined the genetic diversity and demographic history of Bactrocera dorsalis, a destru... more The study examined the genetic diversity and demographic history of Bactrocera dorsalis, a destructive and polyphagous insect pest of fruit crops in diverse geographic regions of India. 19 widely dispersed populations of the fly from India and other Asian countries were analysed using partial sequences of mitochondrial cytochrome oxidase I (cox1) and NADH dehydrogenase 1 (nad1) genes to investigate genetic diversity, genetic structure, and demographic history in the region. Genetic diversity indices [number of haplotypes (H), haloptype diversity (Hd), nucleotide diversity (p) and average number of nucleotide difference (k)] of populations revealed that B. dorsalis maintains fairly high level of genetic diversity without isolation by distance among the geographic regions. Demographic analysis showed significant (negative) Tajimas' D and Fu's F S with non significant sum of squared deviations (SSD) values, which indicate the possibility of recent sudden expansion of species and is further supported through distinctively star-like distribution structure of haplotypes among populations. Thus, the results indicate that both ongoing and historical factors have played important role in determining the genetic structure and diversity of the species in India. Consequently, sterile insect technique (SIT) could be a possible management strategy of species in the regions.
Phytoparasitica, 2016
The aim of the present study was to identify the cultivable gut bacteria associated with peach fr... more The aim of the present study was to identify the cultivable gut bacteria associated with peach fruit fly, Bactrocera zonata, and evaluate their potential to attract adults of B. zonata. Based on culture-dependent characterization methods and 16S rRNA gene sequence analysis, bacteria were identified as members of family Enterobacteriaceae (BZM1, Klebsiella oxytoca), Microbacteriacea (BZM2, Microbacterium spp.) and Nocardiaceae (BZM4, Rhodococcus spp.). Molecular phylogeny placed Klebsiella oxytoca within gram negative γ-proteobacteria whereas, Microbacterium spp. and Rhodococcus spp. were clustered under gram positive Actinobacteria group in family Microbacteriacea and Nocardiaceae, respectively. 16S rRNA gene sequence comparison with the available NCBI database sequences further confirmed the characterizations of bacterial symbionts. Population of these bacterial species increased significantly up to the 11 th day after emergence of adults and thereafter it remains constant. Among 3 bacterial symbionts, metabolites produced from K. oxytoca had the highest attraction to the B. zonata adult females over metabolites produced from other bacteria and their combinations in field bioassay. The B. zonata adult male flies attracted to metabolites produced from each bacterial symbionts alone and their combinations were less in number with comparison to the B. zonata adult females. The present study provides the first description of the attractancy potential of metabolites produced by gut microbial community of B. zonata in open field condition. This study results may prompt the development of a female-targeted population control strategy for this fly.
The study examined the genetic diversity and demographic history of Bactrocera dorsalis, a destru... more The study examined the genetic diversity and demographic history of Bactrocera dorsalis, a destructive and polyphagous insect pest of fruit crops in diverse geographic regions of India. 19 widely dispersed populations of the fly from India and other Asian countries were analysed using partial sequences of mitochondrial cytochrome oxidase I (cox1) and NADH dehydrogenase 1 (nad1) genes to investigate genetic diversity, genetic structure, and demographic history in the region. Genetic diversity indices [number of haplotypes (H), haloptype diversity (Hd), nucleotide diversity (π) and average number of nucleotide difference (k)] of populations revealed that B. dorsalis maintains fairly high level of genetic diversity without isolation by distance among the geographic regions. Demographic analysis showed significant (negative) Tajimas' D and Fu's F S with non significant sum of squared deviations (SSD) values, which indicate the possibility of recent sudden expansion of species and is further supported through distinctively star-like distribution structure of haplotypes among populations. Thus, the results indicate that both ongoing and historical factors have played important role in determining the genetic structure and diversity of the species in India. Consequently, sterile insect technique (SIT) could be a possible management strategy of species in the regions.