mohammad irfan Ali - Academia.edu (original) (raw)
Papers by mohammad irfan Ali
Journal of Nanoscience and Nanotechnology, 2009
Increasing strains with resistance to antibiotics and the resulting failure to treat infectious d... more Increasing strains with resistance to antibiotics and the resulting failure to treat infectious diseases is a major challenge in the medical and health fields. Drug resistance, especially in recent decades, had led to a search for different approaches and methods for finding new compounds against bacteria and fungi. Scientists have accepted the idea that nanotechnology is a novel area of science that combines biology, chemistry, and physics (Rezaei-Zarchi et al., 2012; Demir et al., 2014). Nanoparticles have dimensions of 100 nm or less. They have gained remarkable attention because of their unusual properties and the various applications they are suited for, when compared to their bulkier counterparts (Kato, 2011; Metzler et al., 2012). These features have helped spread the use of nanomaterials at a faster rate day by day. They can be used in the fight against germs, diagnosis and cure of diseases, water and air purification, food production, cosmetics, and clothing (Aitken et al., 2006). Silver is the most commonly used engineering nanomaterial in all consumer products (Akinoglu et al., 2014). Silver and copper have long been known to display a strong toxicity against a wide range of microorganisms. Thus, silver-and copper-based compounds have been used intensively in medicine to treat burns and infections. These antimicrobial effects have been studied by many researchers (Mirzajani et al., 2011; Yasa et al., 2012). The antimicrobial potential of these metals led to their use in health-related products (Selvaraj et al., 2011). Research showed the strong antibacterial effect of silver nanoparticles on both gram-positive and gram-negative bacteria, mainly multidrug-resistant strains (Ashrafi et al., 2013; Sangiliyandi et al., 2014). It can be used as an antifungal agent, and this effect has been intensively studied (Jo et al., 2009). Antibacterial properties of copper nanoparticles (CuNPs) have been compared with triclosan, and a strong antibacterial effect has been reported for both (Cubillo et al., 2006). The mechanisms causing nanoparticles to act on bacteria have not yet been fully clarified. However, the four most common theories proposed are: (1) the uptake of free silver ions leads to a disturbance in ATP production and DNA replication. (2) Silver nanoparticles interact
Suresh Gyan Vihar University International Journal of Environment, Science and, 2019
Antimicrobial resistance occurs when microorganisms which were initially sensitive to antimicrobi... more Antimicrobial resistance occurs when microorganisms which were initially sensitive to antimicrobial drugs becomes ineffective when exposed to them by not killing or inhibiting their growth. Despite growing worldwide attention to Antimicrobial resistance, there are substantial limitations in our understanding of the burden, distribution and determinants of Antimicrobial resistance at the population level. Importance of population-based approaches to assess the association between antimicrobial use and Antimicrobial resistance in humans has to be well thought-out. Such approaches are needed to improve our understanding of the development and spread of Antimicrobial resistance in order to inform strategies for the prevention, detection and management of Antimicrobial resistance, and to support the sustainable use of antimicrobials in healthcare.
Suresh Gyan Vihar University International Journal of Environment, Science and, 2018
Cancer stem cells (CSCs) have been suggested as central precursor of tumor initiation, progressio... more Cancer stem cells (CSCs) have been suggested as central precursor of tumor initiation, progression, recurrence, and therapeutic impedance. Thus, identifying stem cells like moities within cancers and understanding their properties is important for the development of efficacious anticancer therapies and diagnostics. Lysed cancer stem cells can be analyze at genome, transcriptome, and proteome level from a single biological sample can provide better understanding. In this review, we have summarized major techniques that can be useful for cancer stem cell expression profiling at genome level, transcriptome and proteome level. Cancer biomarker based genome analysis can provide cancer diagnosis, prognostic, pharmacodynamics. Microarray based transcriptome profiling can provide better understanding at RNA level and also post transcription modifications, that are responsible for cancer. Proteomics techniques include gel-free and gel-based methods. Gel free method include stable isotope labeling by amino acids in cell culture (SILAC), Isobaric tags for relative and absolute quantitation (iTRAQ) and isotope coded affinity tagging (ICAT) is upgraded and better technique for Chemical proteomic, a combination of isotope coded affinity tagging (ICAT) and mass spectrometry, have proposed as powerful tools for identifying drug targets and explaining complex latent mechanisms of drug action against CSCs. Extracted protein can be analyzed by liquid chromatography (LC) coupled to MS/MS. This detection method permits for high resolution separation, thereby precisely revealing differential protein expression profiles. Gel-based methods employ, Two-dimensional difference in gel electrophoresis (2D-DIGE) is a method in which protein samples labeled by different fluorescent dyes are mixed with identical concentrations. A comparison generated 2D-DIGE fluorescence images allows for quantification of each spot. Hence, 2D-DIGE can reduce gel-to-gel variation by allowing simultaneous separation and comparison of several samples on one gel. Molecularly targeted drug delivery by Immunoliposomes, in which molecular targeting of cancer cells via liposomes conjugated with antibody or other ligand.
The present study explores biological synthesis of ZnO nanoparticles (ZnO NPs) using the cell fre... more The present study explores biological synthesis of ZnO nanoparticles (ZnO NPs) using the cell free aqueous extract of Spirulina platensis. Biosynthesized ZnO NPs were characterized by UV-Vis spectroscopy, SEM, TEM, XRD and FTIR studies and finally tested for antibacterial activity. Bio-synthesis using extract of S. platensis showed the formation of well scattered, highly stable, spherical ZnO NPs with an average size of 40-50 nm. The size and morphology of the nanoparticles were confirmed by SEM and TEM analysis. FTIR and UV-Vis spectra showed that proteins and peptides are mainly responsible for the formation and stabilization of ZnO NPs. Furthermore, the synthesized nanoparticles exhibited good antibacterial activity against pathogenic gram-negative i.e. Escherichia Coli-MTCC-9721, Proteus vulgaris-MTCC-7299, Klebsiella pneumonia-MTCC-9751 and gram-positive i.e. Staphylococcus aureus-MTCC-9542, S. epidermidis-MTCC-2639, Bacillus cereus-MTCC-9017 bacteria. The ZnO NPs had shown maximum zone of inhibition (ZOI) i.e. 34.8±1.65 in P. vulgaris. Use of such a biological method provides a simple, cost-effective alternative template for the synthesis of nanomaterials in a large scale that could be great use in biomedical applications.
Copy Journal of Environmental Biology, Vol. 36, 1389-1392, November 2015© Triveni Enterprises, L... more Copy
Journal of Environmental Biology, Vol. 36, 1389-1392, November 2015© Triveni Enterprises, Lucknow (India)
Introduction Spirulina platensis has been commercially used in several countries as health food, feed (Belay, 2002; Becker, 2003), biofertilizers (Vaishampayan et al., 2001), antimicrobial agents (Kumar et al., 2013), anti-cancer drugs, antioxidants (Estrada et al., 2001) and biotechnology (Eriksen, 2008) because of its valuable constituents like phycobilliproteins, carotenoids, vitamins, polysaccharides, minerals and polyunsaturated fatty acids such as gamma-linolenic acid (Khan et al., 2005; Mani et al., 2005). Phycobilliproteins are water soluble and highly fluorescent proteins, very stable at physiological pH. Phycobilliproteins are gaining importance as natural colorants over synthetic colour as they are nontoxic and non-carcinogenic (Chaneva et al., 2007). Among the phycobiliproteins derived from S. platensis, the most abundant is phycocyanin, a brilliant blue colour pigment having great importance because of its various biological and pharmacological properties e.g. antioxidant, antiviral, anticancer (Liu et al., 2000), neuro-protective (Romay et al., 2003), antitumor (Li et al., 2005), radical scavenging and anti inflammatory properties (Vadiraja and Madyastha, 2000). Carotenoids are structurally diverse lipid soluble pigment having
many different biological functions, such as specific coloration, photoprotection, light harvesting and also serving as precursors for many hormones; therefore an important medicinal and biotechnological class of natural pigments (Pulz and Gross, 2004). Many factors affect growth and biopigment accumulation of microalgae, including light (duration and intensity), nutrients availability, pH and temperature (Samuel et al., 2010; Pandey et al., 2011). Composition and content of phycobilliproteins in cyanobacteria are influenced by environmental factors (Chaneva et al., 2007; Hemlata and Fatma, 2009; Simeunovic et al., 2013). Light is an important factor defining optimal conditions for the culture because irradiance directly influences photosynthetic mechanism. A relatively low morning temperature with rapid increase in light intensity can induce photoinhibitory stress, and heating the cultures significantly reduces growth and biopigment accumulation. These factors affect the nutritional value of microalgae (Brown and Hohmann, 2002; Sayegh and Montagnes, 2011). In view of the above, the present work was carried out to assess the influence of photophase (light) and scotophase (dark)
Impact of natural light on growth and biopigment profile of cyanobacteria Spirulina platensis
nakuljasuja@gmail.com
1 2 1 1 1 Gaurav Sharma, Manoj Kumar, Mohammad Irfan Ali, Suman Saranand Nakuleshwar Dut Jasuja* 1School of Sciences, Suresh Gyan Vihar University, Jaipur-302017, Rajasthan, India 2Marine Biotechnology Laboratory, Department of Botany, University of Delhi, North Campus, Delhi- 110007, India *Corresponding Author E-mail:
Abstract
Key words
Cyanobacteria are economically important microorganisms and good source of natural pigments such as chlorophyll, carotenoids and phycobilliproteins. The present research work showed the optimum combination of photophase and scotophase of Spirulina platensis on biomass and chlorophyll-a, carotenoids, phycocyanin, allophycocyanin, and phycoerythrin contents. The study revealed that among all six light conditions tested, the cultures placed at west facing window, receiving natural day light at temperature 30°C showed extremely significant higher biomass (O.D. 3.46 ) and biopigment accumulation Chlorophyll a 8.94 , Carotenoid 1.62 %, phycocyanin 2.26 %, allophycocyanin 2.66 % and phycoerythrin 1.32 % as compared to the standard (Full day natural light), which might be beneficial for large scale production of biopigment.
Background-The present study explores extracellular synthesis of
The present study explores biological synthesis of silver nanoparticles (AgNPs) using the cell-fr... more The present study explores biological synthesis of silver nanoparticles (AgNPs) using the cell-free extract of Spirulina platensis. Biosynthesised AgNPs were characterised by UV-Vis spectroscopy, SEM, TEM, and FTIR analysis and finally evaluated for antibacterial activity. Extracellular synthesis using aqueous extract of S. platensis showed the formation of well scattered, highly stable, spherical AgNPs with an average size of 30–50 nm. The size and morphology of the nanoparticles were confirmed by SEM and TEM analysis. FTIR and UV-Vis spectra showed that biomolecules, proteins and peptides, are mainly responsible for the formation and stabilisation of AgNPs. Furthermore, the synthesised nanoparticles exhibited high antibacterial activity against bacteria. The AgNPs had shown maximum zone of inhibition (ZOI) that is 31.3 ± 1.11 in P. vulgaris. Use of such a microalgal system provides a simple, cost-effective alternative template for the biosynthesis of nanomaterials of silver in a large scale that could be of great use in biomedical applications.
Liver damage is an epidemic and metabolic disorder which is the most important cause of mortality... more Liver damage is an epidemic and metabolic disorder which is the most important cause of mortality and
morbidity globally. Liver injury treatments are important issue of today’s research domain, because of many
allopathic drugs and their toxic influence lead to liver damage. Therefore attention is drawn to the potentials of
medicinal plants that have the hepatoprotective ability to reduce or cure liver disorder. The use of herbal plants
or their primary and secondary metabolites for curing diseases has long being in continuation since ancient times
due to its therapeutic efficacy and safety. Various herbal plants have been investigated for their hepatoprotective
potential to treat different types of liver disorder. Numerous herbal plants and formulations are effective in
treatment of liver disorder. This systemic review mainly is focused on herbal plants as hepatoprotective in
various traditional medicines and explores the herbal plant, isolated active constituent and formulation with
hepatoprotective activity.
The cyanobacterium Spirulina platensis is an attractive source of the biopigment, which is used a... more The cyanobacterium Spirulina platensis is an attractive source of the biopigment, which is used as a natural
colour in food, cosmetic, pharmaceutical products and have tremendous applications in nutraceuticals, therapeutics
and biotechnological research. The present study examines the possibility of increasing the content of Phycocyanin,
Allophycocyanin, Phycoerythrin and Carotenoids under stress conditions including different pH, salinity and carbon
content in S. platensis isolated from Jalmahal, Jaipur (Rajasthan). The production of Phycocyanin, Allophycocyanin
and Phycoerythrin were enhanced with 0.4 M NaCl, pH 7 and Carbon deficiency as compared to standard.
Recent years have seen tremendous progress in nanotechlogy to study and design of nanoparticles t... more Recent years have seen tremendous progress in nanotechlogy to study and design of nanoparticles towards chemical analysis, medical diagnosis and treatment of disease. This review will provide outline the nanoparticles as targeting ligands, imaging tools, therapeutic drugs, antimicrobials and other functionalities directed toward biomedical application. Nanoparticles (NPs) have gained intensive interest because they have special features, such as unusual optical properties, high stability, biological compatibility, controlable morphology, size dispersion, low toxicity and easy surface functionalization. These distinctive aspects have endorsed the development of novel NPs based assays for clinical diagnostics which promise increased sensitivity and specificity towards treatment of disease.
Wastewater released from textile and dye industry can cause serious environmental effects due to ... more Wastewater released from textile and dye industry can cause serious environmental effects due to the presence of toxic dyes and dark coloration. Algae play an important role in bioremediation through photosynthetic ability, nutrient uptake and biosorption of heavy metals. A biological process is a cost effective method and frequently applied for the decolourisation of dyes. The present study showed the potential of various microalgae i.e. Spirulina plantensis, Chlorella vulgaris, Anacystis nidulans and Oscillatoria agardhii to decolorize the textile dyes i.e. Methyl Red, Methyl Orange, crystal Violet, Erichrome black and Malachite Green were studied. Among the four different algal strains, S. plantensis was found to have more decolourisation activity i.e. 77% at pH 5 for the methyl red than other algal species. The maximum percentage of decolourization 73% for crystal violet showed by S. plantensis followed by C. vulgaris at pH 5. But with further increase in pH resulted in reduced percentage of decolorization. Improvement of decolourisation activity at optimum pH could be a basis of exploitation of microalgae as bioremediation of waste water.
Journal of Nanoscience and Nanotechnology, 2009
Increasing strains with resistance to antibiotics and the resulting failure to treat infectious d... more Increasing strains with resistance to antibiotics and the resulting failure to treat infectious diseases is a major challenge in the medical and health fields. Drug resistance, especially in recent decades, had led to a search for different approaches and methods for finding new compounds against bacteria and fungi. Scientists have accepted the idea that nanotechnology is a novel area of science that combines biology, chemistry, and physics (Rezaei-Zarchi et al., 2012; Demir et al., 2014). Nanoparticles have dimensions of 100 nm or less. They have gained remarkable attention because of their unusual properties and the various applications they are suited for, when compared to their bulkier counterparts (Kato, 2011; Metzler et al., 2012). These features have helped spread the use of nanomaterials at a faster rate day by day. They can be used in the fight against germs, diagnosis and cure of diseases, water and air purification, food production, cosmetics, and clothing (Aitken et al., 2006). Silver is the most commonly used engineering nanomaterial in all consumer products (Akinoglu et al., 2014). Silver and copper have long been known to display a strong toxicity against a wide range of microorganisms. Thus, silver-and copper-based compounds have been used intensively in medicine to treat burns and infections. These antimicrobial effects have been studied by many researchers (Mirzajani et al., 2011; Yasa et al., 2012). The antimicrobial potential of these metals led to their use in health-related products (Selvaraj et al., 2011). Research showed the strong antibacterial effect of silver nanoparticles on both gram-positive and gram-negative bacteria, mainly multidrug-resistant strains (Ashrafi et al., 2013; Sangiliyandi et al., 2014). It can be used as an antifungal agent, and this effect has been intensively studied (Jo et al., 2009). Antibacterial properties of copper nanoparticles (CuNPs) have been compared with triclosan, and a strong antibacterial effect has been reported for both (Cubillo et al., 2006). The mechanisms causing nanoparticles to act on bacteria have not yet been fully clarified. However, the four most common theories proposed are: (1) the uptake of free silver ions leads to a disturbance in ATP production and DNA replication. (2) Silver nanoparticles interact
Suresh Gyan Vihar University International Journal of Environment, Science and, 2019
Antimicrobial resistance occurs when microorganisms which were initially sensitive to antimicrobi... more Antimicrobial resistance occurs when microorganisms which were initially sensitive to antimicrobial drugs becomes ineffective when exposed to them by not killing or inhibiting their growth. Despite growing worldwide attention to Antimicrobial resistance, there are substantial limitations in our understanding of the burden, distribution and determinants of Antimicrobial resistance at the population level. Importance of population-based approaches to assess the association between antimicrobial use and Antimicrobial resistance in humans has to be well thought-out. Such approaches are needed to improve our understanding of the development and spread of Antimicrobial resistance in order to inform strategies for the prevention, detection and management of Antimicrobial resistance, and to support the sustainable use of antimicrobials in healthcare.
Suresh Gyan Vihar University International Journal of Environment, Science and, 2018
Cancer stem cells (CSCs) have been suggested as central precursor of tumor initiation, progressio... more Cancer stem cells (CSCs) have been suggested as central precursor of tumor initiation, progression, recurrence, and therapeutic impedance. Thus, identifying stem cells like moities within cancers and understanding their properties is important for the development of efficacious anticancer therapies and diagnostics. Lysed cancer stem cells can be analyze at genome, transcriptome, and proteome level from a single biological sample can provide better understanding. In this review, we have summarized major techniques that can be useful for cancer stem cell expression profiling at genome level, transcriptome and proteome level. Cancer biomarker based genome analysis can provide cancer diagnosis, prognostic, pharmacodynamics. Microarray based transcriptome profiling can provide better understanding at RNA level and also post transcription modifications, that are responsible for cancer. Proteomics techniques include gel-free and gel-based methods. Gel free method include stable isotope labeling by amino acids in cell culture (SILAC), Isobaric tags for relative and absolute quantitation (iTRAQ) and isotope coded affinity tagging (ICAT) is upgraded and better technique for Chemical proteomic, a combination of isotope coded affinity tagging (ICAT) and mass spectrometry, have proposed as powerful tools for identifying drug targets and explaining complex latent mechanisms of drug action against CSCs. Extracted protein can be analyzed by liquid chromatography (LC) coupled to MS/MS. This detection method permits for high resolution separation, thereby precisely revealing differential protein expression profiles. Gel-based methods employ, Two-dimensional difference in gel electrophoresis (2D-DIGE) is a method in which protein samples labeled by different fluorescent dyes are mixed with identical concentrations. A comparison generated 2D-DIGE fluorescence images allows for quantification of each spot. Hence, 2D-DIGE can reduce gel-to-gel variation by allowing simultaneous separation and comparison of several samples on one gel. Molecularly targeted drug delivery by Immunoliposomes, in which molecular targeting of cancer cells via liposomes conjugated with antibody or other ligand.
The present study explores biological synthesis of ZnO nanoparticles (ZnO NPs) using the cell fre... more The present study explores biological synthesis of ZnO nanoparticles (ZnO NPs) using the cell free aqueous extract of Spirulina platensis. Biosynthesized ZnO NPs were characterized by UV-Vis spectroscopy, SEM, TEM, XRD and FTIR studies and finally tested for antibacterial activity. Bio-synthesis using extract of S. platensis showed the formation of well scattered, highly stable, spherical ZnO NPs with an average size of 40-50 nm. The size and morphology of the nanoparticles were confirmed by SEM and TEM analysis. FTIR and UV-Vis spectra showed that proteins and peptides are mainly responsible for the formation and stabilization of ZnO NPs. Furthermore, the synthesized nanoparticles exhibited good antibacterial activity against pathogenic gram-negative i.e. Escherichia Coli-MTCC-9721, Proteus vulgaris-MTCC-7299, Klebsiella pneumonia-MTCC-9751 and gram-positive i.e. Staphylococcus aureus-MTCC-9542, S. epidermidis-MTCC-2639, Bacillus cereus-MTCC-9017 bacteria. The ZnO NPs had shown maximum zone of inhibition (ZOI) i.e. 34.8±1.65 in P. vulgaris. Use of such a biological method provides a simple, cost-effective alternative template for the synthesis of nanomaterials in a large scale that could be great use in biomedical applications.
Copy Journal of Environmental Biology, Vol. 36, 1389-1392, November 2015© Triveni Enterprises, L... more Copy
Journal of Environmental Biology, Vol. 36, 1389-1392, November 2015© Triveni Enterprises, Lucknow (India)
Introduction Spirulina platensis has been commercially used in several countries as health food, feed (Belay, 2002; Becker, 2003), biofertilizers (Vaishampayan et al., 2001), antimicrobial agents (Kumar et al., 2013), anti-cancer drugs, antioxidants (Estrada et al., 2001) and biotechnology (Eriksen, 2008) because of its valuable constituents like phycobilliproteins, carotenoids, vitamins, polysaccharides, minerals and polyunsaturated fatty acids such as gamma-linolenic acid (Khan et al., 2005; Mani et al., 2005). Phycobilliproteins are water soluble and highly fluorescent proteins, very stable at physiological pH. Phycobilliproteins are gaining importance as natural colorants over synthetic colour as they are nontoxic and non-carcinogenic (Chaneva et al., 2007). Among the phycobiliproteins derived from S. platensis, the most abundant is phycocyanin, a brilliant blue colour pigment having great importance because of its various biological and pharmacological properties e.g. antioxidant, antiviral, anticancer (Liu et al., 2000), neuro-protective (Romay et al., 2003), antitumor (Li et al., 2005), radical scavenging and anti inflammatory properties (Vadiraja and Madyastha, 2000). Carotenoids are structurally diverse lipid soluble pigment having
many different biological functions, such as specific coloration, photoprotection, light harvesting and also serving as precursors for many hormones; therefore an important medicinal and biotechnological class of natural pigments (Pulz and Gross, 2004). Many factors affect growth and biopigment accumulation of microalgae, including light (duration and intensity), nutrients availability, pH and temperature (Samuel et al., 2010; Pandey et al., 2011). Composition and content of phycobilliproteins in cyanobacteria are influenced by environmental factors (Chaneva et al., 2007; Hemlata and Fatma, 2009; Simeunovic et al., 2013). Light is an important factor defining optimal conditions for the culture because irradiance directly influences photosynthetic mechanism. A relatively low morning temperature with rapid increase in light intensity can induce photoinhibitory stress, and heating the cultures significantly reduces growth and biopigment accumulation. These factors affect the nutritional value of microalgae (Brown and Hohmann, 2002; Sayegh and Montagnes, 2011). In view of the above, the present work was carried out to assess the influence of photophase (light) and scotophase (dark)
Impact of natural light on growth and biopigment profile of cyanobacteria Spirulina platensis
nakuljasuja@gmail.com
1 2 1 1 1 Gaurav Sharma, Manoj Kumar, Mohammad Irfan Ali, Suman Saranand Nakuleshwar Dut Jasuja* 1School of Sciences, Suresh Gyan Vihar University, Jaipur-302017, Rajasthan, India 2Marine Biotechnology Laboratory, Department of Botany, University of Delhi, North Campus, Delhi- 110007, India *Corresponding Author E-mail:
Abstract
Key words
Cyanobacteria are economically important microorganisms and good source of natural pigments such as chlorophyll, carotenoids and phycobilliproteins. The present research work showed the optimum combination of photophase and scotophase of Spirulina platensis on biomass and chlorophyll-a, carotenoids, phycocyanin, allophycocyanin, and phycoerythrin contents. The study revealed that among all six light conditions tested, the cultures placed at west facing window, receiving natural day light at temperature 30°C showed extremely significant higher biomass (O.D. 3.46 ) and biopigment accumulation Chlorophyll a 8.94 , Carotenoid 1.62 %, phycocyanin 2.26 %, allophycocyanin 2.66 % and phycoerythrin 1.32 % as compared to the standard (Full day natural light), which might be beneficial for large scale production of biopigment.
Background-The present study explores extracellular synthesis of
The present study explores biological synthesis of silver nanoparticles (AgNPs) using the cell-fr... more The present study explores biological synthesis of silver nanoparticles (AgNPs) using the cell-free extract of Spirulina platensis. Biosynthesised AgNPs were characterised by UV-Vis spectroscopy, SEM, TEM, and FTIR analysis and finally evaluated for antibacterial activity. Extracellular synthesis using aqueous extract of S. platensis showed the formation of well scattered, highly stable, spherical AgNPs with an average size of 30–50 nm. The size and morphology of the nanoparticles were confirmed by SEM and TEM analysis. FTIR and UV-Vis spectra showed that biomolecules, proteins and peptides, are mainly responsible for the formation and stabilisation of AgNPs. Furthermore, the synthesised nanoparticles exhibited high antibacterial activity against bacteria. The AgNPs had shown maximum zone of inhibition (ZOI) that is 31.3 ± 1.11 in P. vulgaris. Use of such a microalgal system provides a simple, cost-effective alternative template for the biosynthesis of nanomaterials of silver in a large scale that could be of great use in biomedical applications.
Liver damage is an epidemic and metabolic disorder which is the most important cause of mortality... more Liver damage is an epidemic and metabolic disorder which is the most important cause of mortality and
morbidity globally. Liver injury treatments are important issue of today’s research domain, because of many
allopathic drugs and their toxic influence lead to liver damage. Therefore attention is drawn to the potentials of
medicinal plants that have the hepatoprotective ability to reduce or cure liver disorder. The use of herbal plants
or their primary and secondary metabolites for curing diseases has long being in continuation since ancient times
due to its therapeutic efficacy and safety. Various herbal plants have been investigated for their hepatoprotective
potential to treat different types of liver disorder. Numerous herbal plants and formulations are effective in
treatment of liver disorder. This systemic review mainly is focused on herbal plants as hepatoprotective in
various traditional medicines and explores the herbal plant, isolated active constituent and formulation with
hepatoprotective activity.
The cyanobacterium Spirulina platensis is an attractive source of the biopigment, which is used a... more The cyanobacterium Spirulina platensis is an attractive source of the biopigment, which is used as a natural
colour in food, cosmetic, pharmaceutical products and have tremendous applications in nutraceuticals, therapeutics
and biotechnological research. The present study examines the possibility of increasing the content of Phycocyanin,
Allophycocyanin, Phycoerythrin and Carotenoids under stress conditions including different pH, salinity and carbon
content in S. platensis isolated from Jalmahal, Jaipur (Rajasthan). The production of Phycocyanin, Allophycocyanin
and Phycoerythrin were enhanced with 0.4 M NaCl, pH 7 and Carbon deficiency as compared to standard.
Recent years have seen tremendous progress in nanotechlogy to study and design of nanoparticles t... more Recent years have seen tremendous progress in nanotechlogy to study and design of nanoparticles towards chemical analysis, medical diagnosis and treatment of disease. This review will provide outline the nanoparticles as targeting ligands, imaging tools, therapeutic drugs, antimicrobials and other functionalities directed toward biomedical application. Nanoparticles (NPs) have gained intensive interest because they have special features, such as unusual optical properties, high stability, biological compatibility, controlable morphology, size dispersion, low toxicity and easy surface functionalization. These distinctive aspects have endorsed the development of novel NPs based assays for clinical diagnostics which promise increased sensitivity and specificity towards treatment of disease.
Wastewater released from textile and dye industry can cause serious environmental effects due to ... more Wastewater released from textile and dye industry can cause serious environmental effects due to the presence of toxic dyes and dark coloration. Algae play an important role in bioremediation through photosynthetic ability, nutrient uptake and biosorption of heavy metals. A biological process is a cost effective method and frequently applied for the decolourisation of dyes. The present study showed the potential of various microalgae i.e. Spirulina plantensis, Chlorella vulgaris, Anacystis nidulans and Oscillatoria agardhii to decolorize the textile dyes i.e. Methyl Red, Methyl Orange, crystal Violet, Erichrome black and Malachite Green were studied. Among the four different algal strains, S. plantensis was found to have more decolourisation activity i.e. 77% at pH 5 for the methyl red than other algal species. The maximum percentage of decolourization 73% for crystal violet showed by S. plantensis followed by C. vulgaris at pH 5. But with further increase in pH resulted in reduced percentage of decolorization. Improvement of decolourisation activity at optimum pH could be a basis of exploitation of microalgae as bioremediation of waste water.