mohammad taher Hojjati - Academia.edu (original) (raw)

Papers by mohammad taher Hojjati

European Review for Medical and Pharmacological Sciences, Oct 1, 2010

Objectives: Alpha-thalassemia (alpha-thal) is one of the most common genetic disorders and in som... more Objectives: Alpha-thalassemia (alpha-thal) is one of the most common genetic disorders and in some populations has prevalence as high as 30%. Disorders in hemoglobin (Hb) synthesis lead to mild to severe reduction in alpha-chain synthesis. Diagnosis of alpha-thal by examining fresh blood taken from umbilical cord is a simple and appropriate approach, while in later stages its diagnosis will be difficult and costly. Material and Methods: This study examined the prevalence of alpha-thal gene deletion in neonates in Sari, Iran. Screening study was carried out by examining fresh blood samples obtained from excised umbilical cords of neonates born in Sari hospitals from June 2007 to March 2008. Complete blood count (CBC) was done and Hb electrophoresis and High Performance Liquid Chromatography (HPLC) were performed for detection of Hb Bart's band. For each case two slides were stained by vital stain, 20 and 120 minutes post blood collection. Prevalence of alpha-thal was calculated and statistically analyzed (p <5%). Results: 69 cases out of 680 (10.1%) were positive for Hb Bart's. In 16 out of 69 positive cases (22.3%) the results of two methods, electrophoresis and slide staining were in conformity. In 53 positive cases (77%) there was no visible band in Hb electrophoresis; however Hb Bart's was detected via vital staining method. If the ratio of mean corpuscular volume (MCV) to red blood cell (RBC) count is smaller than 23, risk of alpha-thal is 2.8 fold greater than cases with an MCV/RBC ratio below 23 (p <0.05). None of the cases were reported to be positive for Hb H disease and hydrops fetalis. Conclusions: Considering high prevalence of alpha-thal gene deletions in neonates in Sari hospitals, it is recommended to screen newborns for alpha-thal in this city and similar areas with such a high prevalence. The sensitivity of cellulose acetate electrophoresis and HPLC methods is not adequately high to detect Hb Bart's in all positive cases and staining and examination of peripheral blood slides stained with vital staining is necessary.

Blood Cells Molecules and Diseases, Oct 1, 2013

Introduction: Helicobacter pylori infection is one of the main causes of peptic ulcer. There are ... more Introduction: Helicobacter pylori infection is one of the main causes of peptic ulcer. There are some blood groups acting as receptors for the pathogen. Based on this view and previous attempts, we tried to examine the relationship between Lewis blood group and H. pylori infection. Materials and method: Blood and saliva samples were collected from 60 patients with established peptic ulcer induced by H. pylori. Secretory status of each patient was determined by both direct agglutination and saliva tests. Results: Seventy-two percent of the patients were secretor and expressed Lewis B antigen. This rate in control group was 61%. Statistical analysis showed no significant difference between the two groups. Conclusion: This study did not find any correlation between Le b antigen expression and presence of H. pylori-induced peptic ulcer. It is now recommended that other factors like Lewis x and sialyl Lewis x should be investigated in binding, colonization and virulence of H. pylori infection in the future.

Background and Objective: Hemoglobinopathies are among the most prevalent genetic disorders world... more Background and Objective: Hemoglobinopathies are among the most prevalent genetic disorders worldwide, and occur as a result of mutations in the gene involved in synthesizing hemoglobin chains. By now more than 1000 defects in hemoglobin chains are discovered. Hemoglobin D (Hb D) is one of these disorders, identified by a single nucleotide mutation on codon 121 of beta globin chain. This study was carried out to evaluate Hb D mutations through molecular methods in Mazandaran province of Iran. Materials and Methods: This descriptive laboratory study was done o n 70 patients w ith an electrophoresis band in hemoglobin-S zone in Mazandaran province of Iran during 2010-11. Capillary zone electrophoresis was done to find out Hb D in 51 patients. Subsequently, PCR-RFLP was performed to evaluate the samples at molecular level. Results: Molecular investigation revealed all cases are carriers of hemoglobin D-Punjab. Two patients were shown to be homozygote carriers of the abnormal gene. Conclusion: This study showed all Hb D affected patients were carriers of Hb D Punjab.

Journal of Mazandaran University of Medical Sciences, Aug 10, 2013

Thalassemia is a highly progressive hemolytic anemia with different levels of complexity in patie... more Thalassemia is a highly progressive hemolytic anemia with different levels of complexity in patients. In thalassemia, reduced level of synthesis of hemoglobin chains results in an imbalanced production of alpha and beta globin chains, and sedimentation of unpaired chains inside red blood cells is the beginning of complications among thalassemia patients. Nowadays compatible blood transfusion is the main approach in treatment of the disease, however, major problems of the patients, results from this treatment, and the most pronounced one is iron overload, which influences the function of different organs including cardiovascular, endocrine and coagulation systems. The major causes of iron overload are non-effective erythropoiesis, frequent blood transfusion, and increased iron absorption through intestine. In order to prevent iron deposition in vital organs, blood transfusions and iron chelation therapy could significantly increase the life expectancy of thalassemia patients. Today, bone marrow transplantation, molecular identification of RBC surface antigens to find the most compatible blood for the patient, and replacing old medications with newly developed ones, are very promising in reducing the dependence of patients on blood transfusion.

Background and objectives: Diagnosis glucose intolerance in pregnancy is very important in preven... more Background and objectives: Diagnosis glucose intolerance in pregnancy is very important in preventing maternal and fetal complications. In this study, we compared hematological and biochemical characteristics of healthy pregnant women and women with gestational diabetes mellitus (GDM) to find predisposing and prognostic variables of GDM. Methods: In this study, 80 pregnant women (at 24-28 weeks of pregnancy) were divided into a GDM group and nonGDM group by performing oral glucose tolerance test using 75 g glucose according to the International Association of the Diabetes and Pregnancy Study Groups criteria. Results: The mean age of women with GDM was significantly higher than those without GDM (p=0.048). Other variables including body mass index, gestational age and daily sleep duration did not differ significantly between the two groups (P>0.05). There was a significant association between family history of diabetes and incidence of GDM (p=0.040). In addition, the C-peptide lev...

Research in Molecular Medicine, 2015

Background: Hepatitis B surface antigen (HBsAg) is one of the main proteins of HBV envelop and it... more Background: Hepatitis B surface antigen (HBsAg) is one of the main proteins of HBV envelop and its serum quantitative measurement is the most common quantitative test for monitoring the progress of Chronic Hepatitis B. Although measurement of serum HBV DNA copy number is a gold standard method for displaying viral load, the test is relatively expensive and it is not readily available everywhere in the world, while quantitative detection of HBsAg is fairly easy and inexpensive. The aim of this study was to investigate the correlation between serum HBsAg level and HBV DNA copy number in patients with chronic HB. Materials and Methods: Quantitative HBsAg, quantitative HBV DNA, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) serum levels were tested in 74 patients with chronic hepatitis B infection-who were HBsAg-positive for more than 6 months. In order to find any correlation between the results of these methods, Spearman and Kruska-Wallis correlation coefficient tests were applied. Results: No significant correlation was observed between quantitative HBsAg and HBV DNA measurements. Also, we could not find any correlation between serum HBsAg and ALT levels. But, serum HBV DNA content and AST level had a significant positive correlation. Conclusion: There are many factors affecting the correlation between serum HBV DNA copy number and HBsAg level such as genotype of HBV virus, phase of infection, methods of measurement, HBeAg status, and drug and types of treatment procedures. Therefore, these factors should be considered in further studies dealing with the correlation between quantitative HBV DNA and HBsAg tests.

Transfusion Clinique et Biologique, 2019

Investigation of correlation between H63D and C282Y mutations in HFE gene and serum Ferritin leve... more Investigation of correlation between H63D and C282Y mutations in HFE gene and serum Ferritin level in beta-thalassemia major patients Étude de la corrélation entre les mutations H63D et C282Y du gène HFE et du taux sérique de ferritine chez des patients bêta-thalassémiques majeurs

DOI: 10.7508/rmm.2015.03.007 Abstract Background: Hepatitis B surface antigen (HBsAg) is one of t... more DOI: 10.7508/rmm.2015.03.007 Abstract Background: Hepatitis B surface antigen (HBsAg) is one of the main proteins of HBV envelop and its serum quantitative measurement is the most common quantitative test for monitoring the progress of Chronic Hepatitis B. Although measurement of serum HBV DNA copy number is a gold standard method for displaying viral load, the test is relatively expensive and it is not readily available everywhere in the world, while quantitative detection of HBsAg is fairly easy and inexpensive. The aim of this study was to investigate the correlation between serum HBsAg level and HBV DNA copy number in patients with chronic HB. Materials and Methods: Quantitative HBsAg, quantitative HBV DNA, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) serum levels were tested in 74 patients with chronic hepatitis B infection who were HBsAg-positive for more than 6 months. In order to find any correlation between the results of these methods, Spearman and K...

Journal of Gorgan University of Medical Sciences, 2014

Background and Objective: Repeated blood transfusion is the major treatment for patients with maj... more Background and Objective: Repeated blood transfusion is the major treatment for patients with major thalassemia. However due to antigen encounters, it may initiate body reactions, including alloantibodies against red blood cell antigens. This study was done to determine the Prevalence of alloimmunization in major beta thalassemia patients in northern Iran. Method: This descriptive - analytic study was carried out on 218 thalassemic patients (100 males and 118 females) with average age of 22.5±7 years in northern Iran during 2010. Each sample was tested for the presence of Alloantibodies including C, Cw, Lea, E, Lua, Leb, K, Jkb, N, P1, D, Jka, M, S, Xga, e, Fya, s, c, Fyb, k, Kpa, Jsb, Lub and Coa. Results: Eighty eight cases (40.4%; 95% CI: 33.9-46.9) were positive for the presence of alloantibodies. Alloantibodies against C, Cw, Lea red blood cell surface antigens were the most prevalent (40%). No significant correlation was found between emergence of alloantibody with the age of ...

Alloimmunisation, a reaction of the immune system to foreign antigens, is one of the most importa... more Alloimmunisation, a reaction of the immune system
to foreign antigens, is one of the most important side
effects of regular blood transfusions. The rate of red
blood cell alloimmunisation following the transfusion
of a single unit of blood is 1 to 1.6%, while the rate
in patients receiving regular blood transfusions may
be as high as 60%.This study showed that up to 47% of our patients
had at least one type of alloantibody

European Review for Medical and Pharmacological Sciences, Oct 1, 2010

Objectives: Alpha-thalassemia (alpha-thal) is one of the most common genetic disorders and in som... more Objectives: Alpha-thalassemia (alpha-thal) is one of the most common genetic disorders and in some populations has prevalence as high as 30%. Disorders in hemoglobin (Hb) synthesis lead to mild to severe reduction in alpha-chain synthesis. Diagnosis of alpha-thal by examining fresh blood taken from umbilical cord is a simple and appropriate approach, while in later stages its diagnosis will be difficult and costly. Material and Methods: This study examined the prevalence of alpha-thal gene deletion in neonates in Sari, Iran. Screening study was carried out by examining fresh blood samples obtained from excised umbilical cords of neonates born in Sari hospitals from June 2007 to March 2008. Complete blood count (CBC) was done and Hb electrophoresis and High Performance Liquid Chromatography (HPLC) were performed for detection of Hb Bart's band. For each case two slides were stained by vital stain, 20 and 120 minutes post blood collection. Prevalence of alpha-thal was calculated and statistically analyzed (p <5%). Results: 69 cases out of 680 (10.1%) were positive for Hb Bart's. In 16 out of 69 positive cases (22.3%) the results of two methods, electrophoresis and slide staining were in conformity. In 53 positive cases (77%) there was no visible band in Hb electrophoresis; however Hb Bart's was detected via vital staining method. If the ratio of mean corpuscular volume (MCV) to red blood cell (RBC) count is smaller than 23, risk of alpha-thal is 2.8 fold greater than cases with an MCV/RBC ratio below 23 (p <0.05). None of the cases were reported to be positive for Hb H disease and hydrops fetalis. Conclusions: Considering high prevalence of alpha-thal gene deletions in neonates in Sari hospitals, it is recommended to screen newborns for alpha-thal in this city and similar areas with such a high prevalence. The sensitivity of cellulose acetate electrophoresis and HPLC methods is not adequately high to detect Hb Bart's in all positive cases and staining and examination of peripheral blood slides stained with vital staining is necessary.

Blood Cells Molecules and Diseases, Oct 1, 2013

Introduction: Helicobacter pylori infection is one of the main causes of peptic ulcer. There are ... more Introduction: Helicobacter pylori infection is one of the main causes of peptic ulcer. There are some blood groups acting as receptors for the pathogen. Based on this view and previous attempts, we tried to examine the relationship between Lewis blood group and H. pylori infection. Materials and method: Blood and saliva samples were collected from 60 patients with established peptic ulcer induced by H. pylori. Secretory status of each patient was determined by both direct agglutination and saliva tests. Results: Seventy-two percent of the patients were secretor and expressed Lewis B antigen. This rate in control group was 61%. Statistical analysis showed no significant difference between the two groups. Conclusion: This study did not find any correlation between Le b antigen expression and presence of H. pylori-induced peptic ulcer. It is now recommended that other factors like Lewis x and sialyl Lewis x should be investigated in binding, colonization and virulence of H. pylori infection in the future.

Background and Objective: Hemoglobinopathies are among the most prevalent genetic disorders world... more Background and Objective: Hemoglobinopathies are among the most prevalent genetic disorders worldwide, and occur as a result of mutations in the gene involved in synthesizing hemoglobin chains. By now more than 1000 defects in hemoglobin chains are discovered. Hemoglobin D (Hb D) is one of these disorders, identified by a single nucleotide mutation on codon 121 of beta globin chain. This study was carried out to evaluate Hb D mutations through molecular methods in Mazandaran province of Iran. Materials and Methods: This descriptive laboratory study was done o n 70 patients w ith an electrophoresis band in hemoglobin-S zone in Mazandaran province of Iran during 2010-11. Capillary zone electrophoresis was done to find out Hb D in 51 patients. Subsequently, PCR-RFLP was performed to evaluate the samples at molecular level. Results: Molecular investigation revealed all cases are carriers of hemoglobin D-Punjab. Two patients were shown to be homozygote carriers of the abnormal gene. Conclusion: This study showed all Hb D affected patients were carriers of Hb D Punjab.

Journal of Mazandaran University of Medical Sciences, Aug 10, 2013

Thalassemia is a highly progressive hemolytic anemia with different levels of complexity in patie... more Thalassemia is a highly progressive hemolytic anemia with different levels of complexity in patients. In thalassemia, reduced level of synthesis of hemoglobin chains results in an imbalanced production of alpha and beta globin chains, and sedimentation of unpaired chains inside red blood cells is the beginning of complications among thalassemia patients. Nowadays compatible blood transfusion is the main approach in treatment of the disease, however, major problems of the patients, results from this treatment, and the most pronounced one is iron overload, which influences the function of different organs including cardiovascular, endocrine and coagulation systems. The major causes of iron overload are non-effective erythropoiesis, frequent blood transfusion, and increased iron absorption through intestine. In order to prevent iron deposition in vital organs, blood transfusions and iron chelation therapy could significantly increase the life expectancy of thalassemia patients. Today, bone marrow transplantation, molecular identification of RBC surface antigens to find the most compatible blood for the patient, and replacing old medications with newly developed ones, are very promising in reducing the dependence of patients on blood transfusion.

Background and objectives: Diagnosis glucose intolerance in pregnancy is very important in preven... more Background and objectives: Diagnosis glucose intolerance in pregnancy is very important in preventing maternal and fetal complications. In this study, we compared hematological and biochemical characteristics of healthy pregnant women and women with gestational diabetes mellitus (GDM) to find predisposing and prognostic variables of GDM. Methods: In this study, 80 pregnant women (at 24-28 weeks of pregnancy) were divided into a GDM group and nonGDM group by performing oral glucose tolerance test using 75 g glucose according to the International Association of the Diabetes and Pregnancy Study Groups criteria. Results: The mean age of women with GDM was significantly higher than those without GDM (p=0.048). Other variables including body mass index, gestational age and daily sleep duration did not differ significantly between the two groups (P>0.05). There was a significant association between family history of diabetes and incidence of GDM (p=0.040). In addition, the C-peptide lev...

Research in Molecular Medicine, 2015

Background: Hepatitis B surface antigen (HBsAg) is one of the main proteins of HBV envelop and it... more Background: Hepatitis B surface antigen (HBsAg) is one of the main proteins of HBV envelop and its serum quantitative measurement is the most common quantitative test for monitoring the progress of Chronic Hepatitis B. Although measurement of serum HBV DNA copy number is a gold standard method for displaying viral load, the test is relatively expensive and it is not readily available everywhere in the world, while quantitative detection of HBsAg is fairly easy and inexpensive. The aim of this study was to investigate the correlation between serum HBsAg level and HBV DNA copy number in patients with chronic HB. Materials and Methods: Quantitative HBsAg, quantitative HBV DNA, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) serum levels were tested in 74 patients with chronic hepatitis B infection-who were HBsAg-positive for more than 6 months. In order to find any correlation between the results of these methods, Spearman and Kruska-Wallis correlation coefficient tests were applied. Results: No significant correlation was observed between quantitative HBsAg and HBV DNA measurements. Also, we could not find any correlation between serum HBsAg and ALT levels. But, serum HBV DNA content and AST level had a significant positive correlation. Conclusion: There are many factors affecting the correlation between serum HBV DNA copy number and HBsAg level such as genotype of HBV virus, phase of infection, methods of measurement, HBeAg status, and drug and types of treatment procedures. Therefore, these factors should be considered in further studies dealing with the correlation between quantitative HBV DNA and HBsAg tests.

Transfusion Clinique et Biologique, 2019

Investigation of correlation between H63D and C282Y mutations in HFE gene and serum Ferritin leve... more Investigation of correlation between H63D and C282Y mutations in HFE gene and serum Ferritin level in beta-thalassemia major patients Étude de la corrélation entre les mutations H63D et C282Y du gène HFE et du taux sérique de ferritine chez des patients bêta-thalassémiques majeurs

DOI: 10.7508/rmm.2015.03.007 Abstract Background: Hepatitis B surface antigen (HBsAg) is one of t... more DOI: 10.7508/rmm.2015.03.007 Abstract Background: Hepatitis B surface antigen (HBsAg) is one of the main proteins of HBV envelop and its serum quantitative measurement is the most common quantitative test for monitoring the progress of Chronic Hepatitis B. Although measurement of serum HBV DNA copy number is a gold standard method for displaying viral load, the test is relatively expensive and it is not readily available everywhere in the world, while quantitative detection of HBsAg is fairly easy and inexpensive. The aim of this study was to investigate the correlation between serum HBsAg level and HBV DNA copy number in patients with chronic HB. Materials and Methods: Quantitative HBsAg, quantitative HBV DNA, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) serum levels were tested in 74 patients with chronic hepatitis B infection who were HBsAg-positive for more than 6 months. In order to find any correlation between the results of these methods, Spearman and K...

Journal of Gorgan University of Medical Sciences, 2014

Background and Objective: Repeated blood transfusion is the major treatment for patients with maj... more Background and Objective: Repeated blood transfusion is the major treatment for patients with major thalassemia. However due to antigen encounters, it may initiate body reactions, including alloantibodies against red blood cell antigens. This study was done to determine the Prevalence of alloimmunization in major beta thalassemia patients in northern Iran. Method: This descriptive - analytic study was carried out on 218 thalassemic patients (100 males and 118 females) with average age of 22.5±7 years in northern Iran during 2010. Each sample was tested for the presence of Alloantibodies including C, Cw, Lea, E, Lua, Leb, K, Jkb, N, P1, D, Jka, M, S, Xga, e, Fya, s, c, Fyb, k, Kpa, Jsb, Lub and Coa. Results: Eighty eight cases (40.4%; 95% CI: 33.9-46.9) were positive for the presence of alloantibodies. Alloantibodies against C, Cw, Lea red blood cell surface antigens were the most prevalent (40%). No significant correlation was found between emergence of alloantibody with the age of ...

Alloimmunisation, a reaction of the immune system to foreign antigens, is one of the most importa... more Alloimmunisation, a reaction of the immune system
to foreign antigens, is one of the most important side
effects of regular blood transfusions. The rate of red
blood cell alloimmunisation following the transfusion
of a single unit of blood is 1 to 1.6%, while the rate
in patients receiving regular blood transfusions may
be as high as 60%.This study showed that up to 47% of our patients
had at least one type of alloantibody