mohammed silwadi - Academia.edu (original) (raw)

Papers by mohammed silwadi

Research paper thumbnail of Enhancing biogas production by anaerobic digestion of animal manure

International Journal of Green Energy, 2022

Research paper thumbnail of Variation in Methane Concentration Produced from Anaerobically Digested Vegetables

The Journal of Engineering Research [TJER], 2015

Anaerobic fermentation is a highly promising technology for converting biomass waste into methane... more Anaerobic fermentation is a highly promising technology for converting biomass waste into methane, which then may directly be used as an energy source. Attempts have been made to optimize various parameters in order to determine the most favorable recipe for maximum biogas production from fermented vegetable waste. The biogas production from many types of vegetable waste such as zucchini, orange peel, tomato, potato, and rice was studied in batch digesters. The effect of adding chicken dung and sludge to vegetable waste on the concentration of methane in the produced biogas was investigated. The experiments were conducted at room temperature (20°C) and at 35°C. The results revealed that methane concentration goes through maximum value with time. This maximum value is obtained faster when the rate of digestion is faster. The concentrations of methane in the biogas produced are ranked as follows: potato>rice>tomato>zucchini>orange peels. The concentrations of methane gas i...

Research paper thumbnail of Extractive—spectrophotometric determination of americium at trace levels with arsenazo-III

Analytica Chimica Acta, 1982

Research paper thumbnail of Extractive spectrophotometric determination of certain dibenzazepine antidepressants in pharmaceutical preparations

A simple, rapid and selective method for the extractive spectrophotometric determination of diben... more A simple, rapid and selective method for the extractive spectrophotometric determination of dibenzazepines, viz., imipramine hydrochloride (IPH), desipramine hydrochloride (DPH), clomipramine hydrochloride (CPH), trimipramine maleate (TPM) and opipramol (OPP) has been worked out using the acidic dye, thymol blue, as the ion-pairing agent in an aqueous medium (pH 7). The yellow ion-pair chromogen being extractable with chloroform could be measured quantitatively at 420 nm. The method obeys Beer's law between 4-44, 4-44, 4-26, 4-24 and 4-36 μg mL - 1 with molar absorptivity of 3.88 × 10 3 , 3.36 × 10 3 , 2.50 × 10 3 , 3.30 × 10 3 and 3.63 x 10 3 L mol - 1 cm - 1 for IPH, DPH, CPH, TPM and OPP, respectively. The commonly encountered excipients and additives do not interfere with the determination. Dibenzazepine drugs (20.0 μg mL - 1 ) can be determined with the relative standard deviation of 1.2 to 2.8% for five replicate measurements. Analyses of preformulations and commercial tab...

Research paper thumbnail of A New Spectrophotometric Method for Determination of Iron in Herbs, Spices and Beans with 2,6-Diacetylprydine Dioxime and 2-Acetylpyridine Monoxime

The reaction of iron (II) with 2, 6-diacetylprydine dioxime (H2dapd) and 2-acetylprydine monoxime... more The reaction of iron (II) with 2, 6-diacetylprydine dioxime (H2dapd) and 2-acetylprydine monoxime (Hapm) in 10% and 60% v/v ethanol-water solution at pH 2.5 and 7.5, respectively, were studied using direct and first derivative spectrophotometry. Fe (III) is reduced by H2dapd and gives iron (II) complex. A simple, rapid, selective and sensitive method for the determination of Fe (II), Fe (III) and a mixture of them with the H2dapd reagent in acid water-ethanol medium after the solution attained to stand periods of 30 sec, 4 hr and 4 hr, respectively, are proposed. Hapm also reacts with Fe (II) to form [Fe(Hapm)3]2+ complex with less selectivity and sensitivity than H2dapm reagent. Calibration graph with [Fe(H2dapd)2]2+ is linear over the range 0.28 ?g mL?1 with an apparent molar absorptivity of 8.481×103 L mol?1cm?1 at ?m 428 nm. Linear dynamic ranges are 0.01-11.0 and 0.07-11.0 ?g mL?1 iron (II) as [Fe(H2dapd)2]2+ complex for direct and derivative modes, respectively. The analytical...

Research paper thumbnail of Sensitive Spectrophotometric Determination of Some Dibenzazepine Drugs with Diazotized P-Phenylenediamine Dihydrochloride

Analytical Letters, 2000

A simple, sensitive and selective spectrophotometric procedure was developed for the determinatio... more A simple, sensitive and selective spectrophotometric procedure was developed for the determination of imipramine hydrochloride, desipramine hydrochloride, clomipramine hydrochloride and trimipramine maleate belonging to dibenzazepine class of drugs. The method is based on the interaction of diazotized p-phenylenediamine dihydrochloride with the drug in sulphuric acid medium. The resulting chromophore was measured at 565 nm, and was stable for about 2.5 hr. The commonly encountered excipients and additives do not interfere with the determination. Dibenzazepine drugs can be determined in the range of 0.1-4.0 μg/ml, with a relative standard deviation of 1.92% for ten replicate measurement of 2.0 μg/ml dibenzazepine drugs. Results from the analysis of preformulations and commercial tablets by this procedure agree well with those of the official method.

Research paper thumbnail of The <I>In Vitro</I> Therapeutic Activity of Ellagic Acid-Alginate-Silver Nanoparticles on Breast Cancer Cells (MCF-7) and Normal Fibroblast Cells (3T3)

Science of Advanced Materials, 2016

The present work involves the development of EA-Alg-AgNPs nanocomposite based on ellagic acid (EA... more The present work involves the development of EA-Alg-AgNPs nanocomposite based on ellagic acid (EA) as active compound. Silver nitrate was taken as the metal precursor (AgNPs) and sodium alginate (Alg) as a reducing agent. The EA-Alg-AgNPs nanocomposite was characterized using transmission electron microscopy (TEM), zeta potential, and in vitro release kinetics. The particles thus obtained were spherical in shape and having an average particles size of 10 nm, zeta potentials of-8.2 mV, and the release kinetics of EA from nanocomposite was following Hixson-Crowell kinetics models with R 2 = 0.9956. The cytotoxicity potential of free EA, Alg-AgNPs and the EA-Alg-AgNPs nanocomposite may be determined using a normal mouse fibroblast cells (3T3) and breast cancer cells (MCF-7). EA-Alg-AgNPs nanocomposite demonstrated a increased cytotoxicity effect when compared to free EA on MCF-7 cells with 15.3% cell viability at 128 μg/mL; compared to 33.5% cell viability in a direct EA exposure. It is worth mentioning the cytotoxicity of Alg-AgNPs against MCF-7 shows 28% viability at 128 μg/mL.

Research paper thumbnail of Sensitive and Selective Spectrophotometric Determination of Hg(II), Ni(II), Cu(II) and Co(II) Using Iminodibenzyl and 3-Chloroiminodibenzyl as New Reagents and Their Applications to Industrial Effluents and Soil Samples

Http Dx Doi Org 10 1080 03067310290024300, Sep 17, 2010

Research paper thumbnail of New spectrophotometric method for the determination of flutamide in pharmaceutical preparations

Journal of Pharmaceutical and Biomedical Analysis, Sep 30, 2000

A sensitive and simple spectrophotometric method for the determinations of reduction product of f... more A sensitive and simple spectrophotometric method for the determinations of reduction product of flutamide (FLA) is described. The method is based on the interaction of diazotized flutamide reduction product with N-(1-naphthyl) ethylenediamine dihydrochloride (NEDA) in neutral or resorcinol (RSL) in alkaline medium. Absorbance of the resulting chromophores is measured at 525 or 480 nm, respectively, and is stable for at

Research paper thumbnail of Extractive spectrophotometric determination of certain dibenzazepine antidepressants in pharmaceutical preparations

Research paper thumbnail of New spectrophotometric method for the determination of reducing sugars

Journal de pharmacie de Belgique

A sensitive and simple spectrophotometric method for the determinations of reduction product of f... more A sensitive and simple spectrophotometric method for the determinations of reduction product of flutamide (FLA) is described. The method is based on the interaction of diazotized flutamide reduction product with N-(1-naphthyl) ethylenediamine dihydrochloride (NEDA) in neutral or resorcinol (RSL) in alkaline medium. Absorbance of the resulting chromophores is measured at 525 or 480 nm, respectively, and is stable for at

Research paper thumbnail of New and Sensitive Method for the Spectrophotometric Determination of Certain Tricyclic Antidepressants

Microchimica Acta, 2000

A new, simple, and sensitive spectrophotometric method for the determination of certain tricyclic... more A new, simple, and sensitive spectrophotometric method for the determination of certain tricyclic antidepressants, belonging to the dibenzazepine class of drugs, has been developed. The proposed method is based on the reaction of imipramine hydrochloride (IPH), desipramine hydrochloride (DPH), clomipramine hydrochloride (CPH), trimipramine maleate (TPM) or opipramol (OPP) with iron(III), and subsequent reaction with ferricyanide in an acetic acid medium, to yield a blue product, with maximum absorption at 720±730 nm.

Research paper thumbnail of Imipramine hydrochloride and desipramine hydrochloride as new reagents for detection of microamounts of blood in urine

Journal of Pharmaceutical and Biomedical Analysis, 2002

Benzidine and o-tolidine, the hazardous carcinogens are still in use for the detection of blood i... more Benzidine and o-tolidine, the hazardous carcinogens are still in use for the detection of blood in urine. Development of safer substitutes are of paramount importance. Unfortunately, the alternate available reagents lack specificity, sensitivity and reproducibility. Imipramine hydrochloride (IPH) and desipramine hydrochloride (DPH) are proposed as new reagents for the detection of blood in urine. Both the reagents impact to blood a green-blue to blue color, in the presence of acetic acid and hydrogen peroxide and the development of this color is explored under laboratory conditions. IPH or DPH compares favorably with benzidine and multistix methods. Both the reagents are relatively inexpensive, and have required sensitivity and stability. The proposed reagents offer advantages of relatively low sensitivity to endogenous vegetable peroxidases; hence, the test can be performed on samples taken from patients who are on a normal diet and the results are reproducible. Besides, the reagents, are non-carcinogenic and can replace benzidine and o-tolidine without any extra procedural difficulties as they also exhibit a similar blue color.

Research paper thumbnail of New reagents for detection of faecal occult blood

Journal of Pharmaceutical and Biomedical Analysis, 2001

Imipramine hydrochloride (IPH) and desipramine hydrochloride (DPH), two widely used antidepressan... more Imipramine hydrochloride (IPH) and desipramine hydrochloride (DPH), two widely used antidepressant drugs, are proposed as new reagents for detection of faecal occult blood. The usefulness of IPH and DPH in occult blood detection has been examined and compared with benzidine and stanoccult methods. The results show that the proposed reagents are selective and sensitive and gives reproducible results. The proposed methodology is much less subject to vegetable peroxidase, iron and vitamin C interference and can be performed on patients who are on a normal diet.

Research paper thumbnail of Detection and diagnosis of blood in feces and urine: an overview

Clinica Chimica Acta, 2002

Background: Detection of blood in feces or urine has long been regarded as an indicator of patien... more Background: Detection of blood in feces or urine has long been regarded as an indicator of patient's state of health. The ease with which feces or urine may be obtained and patient's willingness to provide the specimen make detection of fecal occult blood or urine analysis one of the most commonly performed screening examinations. Historically, the inspection of feces or urine for diagnostic purpose has been practiced for centuries. Of late, management of renal or urinary tract abnormalities or investigation of anemia, gastrointestinal diseases and for early detection of colorectal cancer has assumed greater importance. Methods: The never-ending list of techniques for the diagnosis of disorders producing bleeding such as urine microscopy, urine cytology, urine based marker test, cytoscopy, ultra sonography, computed tomography, magnetic resonance imaging, to mention a few, and four categories of detection of fecal occult blood namely, radioanalytical, physical, immunochemical and chemical methods makes the study very interesting. Purpose: This review attempts to overview various techniques, methods and methodologies for the diagnosis and detection of blood in feces and urine, in the direction of looking at past and current tests with an eye on future needs.

Research paper thumbnail of New reagents for detection of faecal occult blood

Journal of pharmaceutical and …, 2001

Imipramine hydrochloride (IPH) and desipramine hydrochloride (DPH), two widely used antidepressan... more Imipramine hydrochloride (IPH) and desipramine hydrochloride (DPH), two widely used antidepressant drugs, are proposed as new reagents for detection of faecal occult blood. The usefulness of IPH and DPH in occult blood detection has been examined and compared with benzidine and stanoccult methods. The results show that the proposed reagents are selective and sensitive and gives reproducible results. The proposed methodology is much less subject to vegetable peroxidase, iron and vitamin C interference and can be performed on patients who are on a normal diet.

Research paper thumbnail of New reagents for detection of faecal occult blood

Journal of pharmaceutical and …, 2001

Imipramine hydrochloride (IPH) and desipramine hydrochloride (DPH), two widely used antidepressan... more Imipramine hydrochloride (IPH) and desipramine hydrochloride (DPH), two widely used antidepressant drugs, are proposed as new reagents for detection of faecal occult blood. The usefulness of IPH and DPH in occult blood detection has been examined and compared with benzidine and stanoccult methods. The results show that the proposed reagents are selective and sensitive and gives reproducible results. The proposed methodology is much less subject to vegetable peroxidase, iron and vitamin C interference and can be performed on patients who are on a normal diet.

Research paper thumbnail of Enhancing biogas production by anaerobic digestion of animal manure

International Journal of Green Energy, 2022

Research paper thumbnail of Variation in Methane Concentration Produced from Anaerobically Digested Vegetables

The Journal of Engineering Research [TJER], 2015

Anaerobic fermentation is a highly promising technology for converting biomass waste into methane... more Anaerobic fermentation is a highly promising technology for converting biomass waste into methane, which then may directly be used as an energy source. Attempts have been made to optimize various parameters in order to determine the most favorable recipe for maximum biogas production from fermented vegetable waste. The biogas production from many types of vegetable waste such as zucchini, orange peel, tomato, potato, and rice was studied in batch digesters. The effect of adding chicken dung and sludge to vegetable waste on the concentration of methane in the produced biogas was investigated. The experiments were conducted at room temperature (20°C) and at 35°C. The results revealed that methane concentration goes through maximum value with time. This maximum value is obtained faster when the rate of digestion is faster. The concentrations of methane in the biogas produced are ranked as follows: potato>rice>tomato>zucchini>orange peels. The concentrations of methane gas i...

Research paper thumbnail of Extractive—spectrophotometric determination of americium at trace levels with arsenazo-III

Analytica Chimica Acta, 1982

Research paper thumbnail of Extractive spectrophotometric determination of certain dibenzazepine antidepressants in pharmaceutical preparations

A simple, rapid and selective method for the extractive spectrophotometric determination of diben... more A simple, rapid and selective method for the extractive spectrophotometric determination of dibenzazepines, viz., imipramine hydrochloride (IPH), desipramine hydrochloride (DPH), clomipramine hydrochloride (CPH), trimipramine maleate (TPM) and opipramol (OPP) has been worked out using the acidic dye, thymol blue, as the ion-pairing agent in an aqueous medium (pH 7). The yellow ion-pair chromogen being extractable with chloroform could be measured quantitatively at 420 nm. The method obeys Beer's law between 4-44, 4-44, 4-26, 4-24 and 4-36 μg mL - 1 with molar absorptivity of 3.88 × 10 3 , 3.36 × 10 3 , 2.50 × 10 3 , 3.30 × 10 3 and 3.63 x 10 3 L mol - 1 cm - 1 for IPH, DPH, CPH, TPM and OPP, respectively. The commonly encountered excipients and additives do not interfere with the determination. Dibenzazepine drugs (20.0 μg mL - 1 ) can be determined with the relative standard deviation of 1.2 to 2.8% for five replicate measurements. Analyses of preformulations and commercial tab...

Research paper thumbnail of A New Spectrophotometric Method for Determination of Iron in Herbs, Spices and Beans with 2,6-Diacetylprydine Dioxime and 2-Acetylpyridine Monoxime

The reaction of iron (II) with 2, 6-diacetylprydine dioxime (H2dapd) and 2-acetylprydine monoxime... more The reaction of iron (II) with 2, 6-diacetylprydine dioxime (H2dapd) and 2-acetylprydine monoxime (Hapm) in 10% and 60% v/v ethanol-water solution at pH 2.5 and 7.5, respectively, were studied using direct and first derivative spectrophotometry. Fe (III) is reduced by H2dapd and gives iron (II) complex. A simple, rapid, selective and sensitive method for the determination of Fe (II), Fe (III) and a mixture of them with the H2dapd reagent in acid water-ethanol medium after the solution attained to stand periods of 30 sec, 4 hr and 4 hr, respectively, are proposed. Hapm also reacts with Fe (II) to form [Fe(Hapm)3]2+ complex with less selectivity and sensitivity than H2dapm reagent. Calibration graph with [Fe(H2dapd)2]2+ is linear over the range 0.28 ?g mL?1 with an apparent molar absorptivity of 8.481×103 L mol?1cm?1 at ?m 428 nm. Linear dynamic ranges are 0.01-11.0 and 0.07-11.0 ?g mL?1 iron (II) as [Fe(H2dapd)2]2+ complex for direct and derivative modes, respectively. The analytical...

Research paper thumbnail of Sensitive Spectrophotometric Determination of Some Dibenzazepine Drugs with Diazotized P-Phenylenediamine Dihydrochloride

Analytical Letters, 2000

A simple, sensitive and selective spectrophotometric procedure was developed for the determinatio... more A simple, sensitive and selective spectrophotometric procedure was developed for the determination of imipramine hydrochloride, desipramine hydrochloride, clomipramine hydrochloride and trimipramine maleate belonging to dibenzazepine class of drugs. The method is based on the interaction of diazotized p-phenylenediamine dihydrochloride with the drug in sulphuric acid medium. The resulting chromophore was measured at 565 nm, and was stable for about 2.5 hr. The commonly encountered excipients and additives do not interfere with the determination. Dibenzazepine drugs can be determined in the range of 0.1-4.0 μg/ml, with a relative standard deviation of 1.92% for ten replicate measurement of 2.0 μg/ml dibenzazepine drugs. Results from the analysis of preformulations and commercial tablets by this procedure agree well with those of the official method.

Research paper thumbnail of The <I>In Vitro</I> Therapeutic Activity of Ellagic Acid-Alginate-Silver Nanoparticles on Breast Cancer Cells (MCF-7) and Normal Fibroblast Cells (3T3)

Science of Advanced Materials, 2016

The present work involves the development of EA-Alg-AgNPs nanocomposite based on ellagic acid (EA... more The present work involves the development of EA-Alg-AgNPs nanocomposite based on ellagic acid (EA) as active compound. Silver nitrate was taken as the metal precursor (AgNPs) and sodium alginate (Alg) as a reducing agent. The EA-Alg-AgNPs nanocomposite was characterized using transmission electron microscopy (TEM), zeta potential, and in vitro release kinetics. The particles thus obtained were spherical in shape and having an average particles size of 10 nm, zeta potentials of-8.2 mV, and the release kinetics of EA from nanocomposite was following Hixson-Crowell kinetics models with R 2 = 0.9956. The cytotoxicity potential of free EA, Alg-AgNPs and the EA-Alg-AgNPs nanocomposite may be determined using a normal mouse fibroblast cells (3T3) and breast cancer cells (MCF-7). EA-Alg-AgNPs nanocomposite demonstrated a increased cytotoxicity effect when compared to free EA on MCF-7 cells with 15.3% cell viability at 128 μg/mL; compared to 33.5% cell viability in a direct EA exposure. It is worth mentioning the cytotoxicity of Alg-AgNPs against MCF-7 shows 28% viability at 128 μg/mL.

Research paper thumbnail of Sensitive and Selective Spectrophotometric Determination of Hg(II), Ni(II), Cu(II) and Co(II) Using Iminodibenzyl and 3-Chloroiminodibenzyl as New Reagents and Their Applications to Industrial Effluents and Soil Samples

Http Dx Doi Org 10 1080 03067310290024300, Sep 17, 2010

Research paper thumbnail of New spectrophotometric method for the determination of flutamide in pharmaceutical preparations

Journal of Pharmaceutical and Biomedical Analysis, Sep 30, 2000

A sensitive and simple spectrophotometric method for the determinations of reduction product of f... more A sensitive and simple spectrophotometric method for the determinations of reduction product of flutamide (FLA) is described. The method is based on the interaction of diazotized flutamide reduction product with N-(1-naphthyl) ethylenediamine dihydrochloride (NEDA) in neutral or resorcinol (RSL) in alkaline medium. Absorbance of the resulting chromophores is measured at 525 or 480 nm, respectively, and is stable for at

Research paper thumbnail of Extractive spectrophotometric determination of certain dibenzazepine antidepressants in pharmaceutical preparations

Research paper thumbnail of New spectrophotometric method for the determination of reducing sugars

Journal de pharmacie de Belgique

A sensitive and simple spectrophotometric method for the determinations of reduction product of f... more A sensitive and simple spectrophotometric method for the determinations of reduction product of flutamide (FLA) is described. The method is based on the interaction of diazotized flutamide reduction product with N-(1-naphthyl) ethylenediamine dihydrochloride (NEDA) in neutral or resorcinol (RSL) in alkaline medium. Absorbance of the resulting chromophores is measured at 525 or 480 nm, respectively, and is stable for at

Research paper thumbnail of New and Sensitive Method for the Spectrophotometric Determination of Certain Tricyclic Antidepressants

Microchimica Acta, 2000

A new, simple, and sensitive spectrophotometric method for the determination of certain tricyclic... more A new, simple, and sensitive spectrophotometric method for the determination of certain tricyclic antidepressants, belonging to the dibenzazepine class of drugs, has been developed. The proposed method is based on the reaction of imipramine hydrochloride (IPH), desipramine hydrochloride (DPH), clomipramine hydrochloride (CPH), trimipramine maleate (TPM) or opipramol (OPP) with iron(III), and subsequent reaction with ferricyanide in an acetic acid medium, to yield a blue product, with maximum absorption at 720±730 nm.

Research paper thumbnail of Imipramine hydrochloride and desipramine hydrochloride as new reagents for detection of microamounts of blood in urine

Journal of Pharmaceutical and Biomedical Analysis, 2002

Benzidine and o-tolidine, the hazardous carcinogens are still in use for the detection of blood i... more Benzidine and o-tolidine, the hazardous carcinogens are still in use for the detection of blood in urine. Development of safer substitutes are of paramount importance. Unfortunately, the alternate available reagents lack specificity, sensitivity and reproducibility. Imipramine hydrochloride (IPH) and desipramine hydrochloride (DPH) are proposed as new reagents for the detection of blood in urine. Both the reagents impact to blood a green-blue to blue color, in the presence of acetic acid and hydrogen peroxide and the development of this color is explored under laboratory conditions. IPH or DPH compares favorably with benzidine and multistix methods. Both the reagents are relatively inexpensive, and have required sensitivity and stability. The proposed reagents offer advantages of relatively low sensitivity to endogenous vegetable peroxidases; hence, the test can be performed on samples taken from patients who are on a normal diet and the results are reproducible. Besides, the reagents, are non-carcinogenic and can replace benzidine and o-tolidine without any extra procedural difficulties as they also exhibit a similar blue color.

Research paper thumbnail of New reagents for detection of faecal occult blood

Journal of Pharmaceutical and Biomedical Analysis, 2001

Imipramine hydrochloride (IPH) and desipramine hydrochloride (DPH), two widely used antidepressan... more Imipramine hydrochloride (IPH) and desipramine hydrochloride (DPH), two widely used antidepressant drugs, are proposed as new reagents for detection of faecal occult blood. The usefulness of IPH and DPH in occult blood detection has been examined and compared with benzidine and stanoccult methods. The results show that the proposed reagents are selective and sensitive and gives reproducible results. The proposed methodology is much less subject to vegetable peroxidase, iron and vitamin C interference and can be performed on patients who are on a normal diet.

Research paper thumbnail of Detection and diagnosis of blood in feces and urine: an overview

Clinica Chimica Acta, 2002

Background: Detection of blood in feces or urine has long been regarded as an indicator of patien... more Background: Detection of blood in feces or urine has long been regarded as an indicator of patient's state of health. The ease with which feces or urine may be obtained and patient's willingness to provide the specimen make detection of fecal occult blood or urine analysis one of the most commonly performed screening examinations. Historically, the inspection of feces or urine for diagnostic purpose has been practiced for centuries. Of late, management of renal or urinary tract abnormalities or investigation of anemia, gastrointestinal diseases and for early detection of colorectal cancer has assumed greater importance. Methods: The never-ending list of techniques for the diagnosis of disorders producing bleeding such as urine microscopy, urine cytology, urine based marker test, cytoscopy, ultra sonography, computed tomography, magnetic resonance imaging, to mention a few, and four categories of detection of fecal occult blood namely, radioanalytical, physical, immunochemical and chemical methods makes the study very interesting. Purpose: This review attempts to overview various techniques, methods and methodologies for the diagnosis and detection of blood in feces and urine, in the direction of looking at past and current tests with an eye on future needs.

Research paper thumbnail of New reagents for detection of faecal occult blood

Journal of pharmaceutical and …, 2001

Imipramine hydrochloride (IPH) and desipramine hydrochloride (DPH), two widely used antidepressan... more Imipramine hydrochloride (IPH) and desipramine hydrochloride (DPH), two widely used antidepressant drugs, are proposed as new reagents for detection of faecal occult blood. The usefulness of IPH and DPH in occult blood detection has been examined and compared with benzidine and stanoccult methods. The results show that the proposed reagents are selective and sensitive and gives reproducible results. The proposed methodology is much less subject to vegetable peroxidase, iron and vitamin C interference and can be performed on patients who are on a normal diet.

Research paper thumbnail of New reagents for detection of faecal occult blood

Journal of pharmaceutical and …, 2001

Imipramine hydrochloride (IPH) and desipramine hydrochloride (DPH), two widely used antidepressan... more Imipramine hydrochloride (IPH) and desipramine hydrochloride (DPH), two widely used antidepressant drugs, are proposed as new reagents for detection of faecal occult blood. The usefulness of IPH and DPH in occult blood detection has been examined and compared with benzidine and stanoccult methods. The results show that the proposed reagents are selective and sensitive and gives reproducible results. The proposed methodology is much less subject to vegetable peroxidase, iron and vitamin C interference and can be performed on patients who are on a normal diet.