mona taha - Academia.edu (original) (raw)

Papers by mona taha

Research paper thumbnail of Association of heat shock protein70-2 genotypes with hypertension among textile workers occupationally exposed to noise

Environmental Science and Pollution Research

Research paper thumbnail of Neurotoxicity of organic solvents with emphasis on the role of iron

Journal of Complementary and Integrative Medicine

Background Exposure to organic solvents (OS) adversely affects different body systems, the centra... more Background Exposure to organic solvents (OS) adversely affects different body systems, the central and peripheral nervous systems being the most susceptible ones. Objectives This study investigated the role of iron in association with some neurotransmitters for diagnosis of neurotoxicity of OS. Methods The study included 90 workers, 50 occupationally exposed to OS and 40 representing control group. Blood samples were collected from the included subjects for determination of serum iron, total iron binding capacity (TIBC), serotonin and gamma-aminobutyric acid (GABA). Results Revealed reduction in serotonin level and serum iron. However, the elevation in GABA and TIBC was observed. The duration of exposure was significantly correlated with iron and serotonin while it was positively correlated with GABA and TIBC. Conclusions Elevated GABA and TIBC with decreased serotonin and serum iron can be used as early diagnostic measures to detect the neurotoxic effects of OS.

Research paper thumbnail of Association of microsomal epoxide hydrolase gene (fast genotype) with lung functions impairment in wood workers

Journal of Complementary and Integrative Medicine

Objectives Exposure to wood dust may lead to impairment of the lung functions. Microsomal epoxide... more Objectives Exposure to wood dust may lead to impairment of the lung functions. Microsomal epoxide hydrolase enzyme (EPHX1) was shown to take part in protection against oxidative stress. An alteration in enzyme activity might be associated with its gene polymorphisms. In vitro polymorphisms in exons 3 (His113Tyr) and 4 (Arg139His) lead to reduced activity (slow allele) and increased activity (fast allele). Macrophage inflammatory protein 2 (MIP-2) is produced in rat lung epithelial cells after exposure to fine particles. We aimed to investigate the associations between mEPHX1 polymorphisms (in exon 3 and 4) and lung function in furniture workers and assessment of MIP-2 effect. Methods Our study was performed on 70 wood dust exposed male workers and 70 matched normal controls subjects. Ventilatory function tests were measured by spirometer, MIP-2 was performed by ELISA methods and EPHX gene was done by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) metho...

Research paper thumbnail of Hepatotoxicity of aflatoxin B1 and its oxidative effects in wood dust Egyptian exposed workers

Archives of Environmental & Occupational Health

Research paper thumbnail of Epigenetic study of global gene methylation in PON1, XRCC1 and GSTs different genotypes in rural and urban pesticide exposed workers

Journal of Complementary and Integrative Medicine

BackgroundEpigenetic represents a study of occurred heritable gene expression changes without cha... more BackgroundEpigenetic represents a study of occurred heritable gene expression changes without changing in the DNA sequence. It includes DNA methylation and miRNA expression that attract increasing attention as potential links between the genetic and environmental determinants of health and disease. Pesticide exposure is associated with adverse health effects and DNA methylation due to oxidative stress induced following its exposure. This study aimed to define the association of genetic polymorphisms of XRCC1, PON1, GSTP1 and GST genes with global genes DNA methylation in urban and rural occupationally pesticides exposed workers.MethodsThis study included 100 pesticides exposed workers; 50 rural sprayers (RE) and 50 urban researchers (UE). Controls included equal numbers. DNA methylation of global genes was evaluated by pyrosequencing assay. XRCC1, PON1 and GSTP1 genotyping were assessed by PCR–RFLP, and GST M1 and T1 were performed by PCR.ResultsThe results of this study revealed th...

Research paper thumbnail of Audiometric notch for the prediction of early occupational hearing loss and its association with the interleukin-1beta genotype

Journal of Taibah University Medical Sciences

Research paper thumbnail of Early prediction of liver carcinogenicity due to occupational exposure to pesticides

Mutation Research/Genetic Toxicology and Environmental Mutagenesis

Research paper thumbnail of Impact of occupational cadmium exposure on bone in sewage workers

International Journal of Occupational and Environmental Health

Research paper thumbnail of Assessment of Anticholinesterase Toxicity, Oxidative Stress and Antioxidant Status in Carbamate and Organophosphorus PesticidesExposed Agricultural Workers

International Journal of Pharmaceutical and Clinical Research

Among the numerous pesticides, anticholinesterase compounds are widely used. Their toxicity induc... more Among the numerous pesticides, anticholinesterase compounds are widely used. Their toxicity induced by cholinesterase inhibition at the synapses and neuromuscular junctions, leading to neurological disorders. Inhibition of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) has been used as sensitive biomarkers for pesticides exposure. In the present study, AChE and BuChE levels were estimated in agricultural workers exposed to carbamate and organophosphorus pesticides with average 9.8±3.5 years relative to the controls. The toxic effects of pesticides may be attributed to induction of oxidative stress and alteration in antioxidant system. Our results showed significant decrease in AChE and BuChE levels with inhibition percentage of 39% and 61% respectively, in exposed workers than controls. Additionally, there was a significant increase in malondialdehyde (MDA) as an oxidative stress marker. Concerning antioxidant status, there was significant decrease in reduced glutathi...

Research paper thumbnail of GSTP1 and XRCC1 polymorphisms and DNA damage in agricultural workers exposed to pesticides

Mutation Research/Genetic Toxicology and Environmental Mutagenesis

Pesticide exposure may be associated with increased risk of genotoxicity and carcinogenesis. Thes... more Pesticide exposure may be associated with increased risk of genotoxicity and carcinogenesis. These risks may be affected by polymorphisms of genes for glutathione transferase-dependent metabolism of pesticides and for DNA repair. We studied the prevalence of GSTP1 and XRCC1 polymorphisms and their possible correlation with DNA damage following prolonged pesticide exposure. DNA damage was estimated by the comet assay in peripheral blood samples from 51 pesticide-exposed workers and 50 controls. GSTP1 (105) and XRCC1 (399 and 194) genotypes were identified by restriction fragment length analysis. Individuals carrying theGSTP1 Ile-Ile or XRCC1399 Arg-Arg genotypes showed greater DNA damage than observed for other alleles.

Research paper thumbnail of Hepatotoxic Effect of Aflatoxin B1 and Polymorphisms in Glutathione S-Transferase in Millers Occupationally Exposed to Aspergillus

Little is known whether occupational exposure to aflatoxin might have a potential hazard. The pre... more Little is known whether occupational exposure to aflatoxin might have a potential hazard. The present work aimed to study the hepatotoxic effects of occupational exposure to AFB1 as a metabolite of high Aspergillus concentrations in the working environments, and the polymorphism of GST gene in exposed workers. The study was performed on 97 flour mill workers exposed to high Aspergillus concentrations and 78 non-exposed controls. The levels of AFB1/Alb (ng/g), AST and ALT of the workers were significantly higher than the controls, while there was no significance difference in the ALP levels between the two groups. The present results revealed that AFB1/Alb levels were significantly higher in the workers with the different GST alleles compared to the control groups. Additionally, AFB1/Alb levels were significantly higher in the workers with GSTT1 compared to the workers with different GST alleles (GSTM1 and Null (GSTT1&GSTM1)) and the controls with Null (GSTT1&GSTM1) alleles. In conclusion, occupational exposure to the high concentrations of Aspergillus in the workplace may cause an increase in the AFB1/Alb and the liver enzymes in flour mill workers. These results also showed that the Null (GSTM1 & GSTT1) alleles are the most common type in the studied population. The workers with GSTT1 have lower ability to detoxify AFB1.

Research paper thumbnail of Gastritis Induced by Helicobacter pylori Infection in Experimental Rats

Digestive Diseases and Sciences, Jan 22, 2010

Background Gastritis, an inflammation of gastric mucosa, may be due to many pathological factors ... more Background Gastritis, an inflammation of gastric mucosa, may be due to many pathological factors and infection, such as with Helicobacter pylori. The use of experimental models of gastritis is important to evaluate the biochemical changes and study chemotherapeutic intervention. In a previous study we demonstrated an acute gastritis model induced by iodoacetamide. Aims Our objective in this study was to evaluate a new gastritis model induced by H. pylori infection in experimental rats in terms of certain biomarkers in serum and mucosal tissues in addition to histopathological examination. Methods Gastritis was induced in 20 albino Wistar rats by H. pylori isolated from antral biopsy taken from a 49-yearold male patient endoscopically diagnosed as having H. pylori infection. Another ten rats were used as controls. Serum gastrin, pepsinogen I activity, interleukin-6 (IL-6) and gastric mucosal myeloperoxidase (MPO) activity and prostaglandin E 2 (PGE 2) were measured. Immunostaining for inducible nitric oxide synthase (iNOS), nitrotyrosine and DNA fragmentation were used to further evaluate H. pylori-induced gastritis. Results Serum gastrin, IL-6, mucosal MPO activity, and PGE 2 demonstrated significant increases joined with a decreased serum pepsinogen I activity (P \ 0.001). Immunohistochemistry demonstrated positive reaction for iNOS, nitrotyrosine and DNA fragmentation. Conclusions Helicobacter pylori-induced gastritis models demonstrated massive oxidative stress and pronounced injury in mucosal tissue. Since our model in rats reflected the clinical picture of H. pylori infection, it can be considered as a consistent model to study chemotherapeutic intervention for this type of gastritis.

Research paper thumbnail of Comparative Hepatotoxicity of Aflatoxin B1 among Workers Exposed to Different Organic Dust with Emphasis on Polymorphism Role of Glutathione S-Transferase Gene

Open Access Macedonian Journal of Medical Sciences, 2016

AIM: The study aimed to investigate effects of organic dust exposure from different sources on af... more AIM: The study aimed to investigate effects of organic dust exposure from different sources on aflatoxin B1-albumin adducts (AFB1/Alb), and role of glutathione S-transferase (GST) gene polymorphism in hepatotoxicity of (AFB1) among exposed workers. MATERIAL AND METHODS: Liver enzymes, AFB1/Alb, and GST polymorphism were estimated in 132 wheat flour dust and 87 woods sawmill workers, and 156 controls.RESULTS: Results revealed that AFB1/Alb and liver enzymes were significantly elevated in exposed workers compared to controls, and were significantly higher in sawmill workers compared to flour workers. AFB1/Alb in flour and sawmill workers with GSTT1 and GSTM1&GSTT1 null genotypes were significantly higher than controls, and in sawmill workers with GSTM1&GSTT1 null than flour workers. Liver enzymes (ALT and AST) in sawmill workers were significantly higher than flour workers and controls in all GST polymorphism; except in GSTT1 polymorphism, where these enzymes were significantly higher...

Research paper thumbnail of Effect of Helicobacter pylori on the mucosa of the lower end of the esophagus in induced chronic gastritis in adult albino rats

The Egyptian Journal of Histology, 2013

Research paper thumbnail of Hepatotoxic Effect of Aflatoxin B1 and Polymorphisms in Glutathione S-Transferase in Millers Occupationally Exposed to Aspergillus

Little is known whether occupational exposure to aflatoxin might have a potential hazard. The pre... more Little is known whether occupational exposure to aflatoxin might have a potential hazard. The present work aimed to study the hepatotoxic effects of occupational exposure to AFB1 as a metabolite of high Aspergillus concentrations in the working environments, and the polymorphism of GST gene in exposed workers. The study was performed on 97 flour mill workers exposed to high Aspergillus concentrations and 78 non-exposed controls. The levels of AFB1/Alb (ng/g), AST and ALT of the workers were significantly higher than the controls, while there was no significance difference in the ALP levels between the two groups. The present results revealed that AFB1/Alb levels were significantly higher in the workers with the different GST alleles compared to the control groups. Additionally, AFB1/Alb levels were significantly higher in the workers with GSTT1 compared to the workers with different GST alleles (GSTM1 and Null (GSTT1&GSTM1)) and the controls with Null (GSTT1&GSTM1) alleles. In concl...

Research paper thumbnail of Carcinogenic effects of aflatoxin B1 among wheat handlers

International Journal of Occupational and Environmental Health, 2014

Epidemiological studies have demonstrated that serum aflatoxin B1 (AFB1) is a hepatocarcinogenic ... more Epidemiological studies have demonstrated that serum aflatoxin B1 (AFB1) is a hepatocarcinogenic mycotoxin and contributor to the high rate of hepatocellular carcinoma (HCC). The prevalence of liver cancer in Egypt is particularly worrisome. In a registry-based analysis of occupational risk for HCC, significant excesses were observed especially for grain mill workers. The aim of this study was to assess the hepatic carcinogenicity of AFB1 in wheat handlers. Serum AFB1/albumin (AFB1/Alb), alpha-fetoprotein (AFP), alpha-l-fucosidase (AFU), and arginase were estimated in exposed wheat handlers including millers and bakers. The control group was composed of non-occupationally exposed workers. AFB1/Alb and AFU were significantly higher among workers employed as bakers compared to mill workers and controls. Mill workers had higher levels of AFB1/Alb than the controls. AFB1/Alb, AFP, and AFU were all significantly higher and arginase was significantly lower among HCC cases compared to the other groups. There was a significant correlation between AFU and AFB1/Alb in bakers and between AFP and AFB1/Alb in HCC cases. Arginase was inversely correlated with AFB1/Alb in HCC cases. AFB1/Alb was significantly correlated with the duration of exposure in bakers. Wheat handlers exposed to Aspergillus flavus have a high risk of elevated serum AFB1/Alb levels and AFU.

Research paper thumbnail of Effect of Nigella sativa oil versus amoxicillin in induced chronic fundic gastritis in adult female albino rats

The Egyptian Journal of Histology, 2011

Advertisement. Close Window. Close Window. Thank you for choosing to subscribe to the eTOC for Eg... more Advertisement. Close Window. Close Window. Thank you for choosing to subscribe to the eTOC for Egyptian Journal of Histology. Enter your Email address: Wolters Kluwer Health may email you for journal alerts and information ...

Research paper thumbnail of Pattern of gastritis as manipulated by current state of Helicobacter pylori infection

wseas.us

Helicobacter pylori (H. pylori) infection prevails from 60-80% in patients with gastric ulcer and... more Helicobacter pylori (H. pylori) infection prevails from 60-80% in patients with gastric ulcer and 90-100% in those having duodenal ulcer. Patients with such type of chronic infection are at increased risk to develop peptic ulcers or gastric adenocarcinomas. The present work aims mainly to identify the pattern of chronic gastritis and potential effect of H. pylori infection using certain biomarkers, histological and immunochemical tests. Fifty eight individuals, clinically diagnosed as having chronic gastritis, were participated in the present study. They were categorized into 2 groups, the first one (31%) demonstrated positive reaction to IgM antibodies of Helicobacter pylori (H. pylori) (>40u/ml) and the second group (69%) demonstrated negative reaction. Blood and antral biopsy samples were collected, directed to determination of serum gastrin, pepsinogen I (PgI), pepsinogen II (PgII), prostaglandin E 2 (PGE 2) and interlukin-6 (IL-6). Immunohistochemistry technique was also done in antral biopsy to demonstrate the expression of inducible nitric oxide synthase (iNOS), nitrotyrosine, DNA fragmentation, myeloperoxidase and histopathological examination. Serum gastrin, PgI, PgII, PGE 2 , IL-6 demonstrated significant increase in gastritis patients as compared to normal group. PgI, PgII showed significant increase joined with slight increase of IL-6 in IgM positive group as compared to negative one. Immunostaining testes in antral biopsy showed strong positive reactions for the above mentioned markers as compared to IgM negative group (mild positive reaction). In conclusion, gastritis patients who express IgM antibodies for H. pylori infection showed higher gastrinaemia and more pronounced atrophic, inflammatory and apoptotic damage than those not expressing IgM antibodies.

Research paper thumbnail of Gastritis Induced by Helicobacter pylori Infection in Experimental Rats

Digestive diseases and …, 2010

Background Gastritis, an inflammation of gastric mucosa, may be due to many pathological factors ... more Background Gastritis, an inflammation of gastric mucosa, may be due to many pathological factors and infection, such as with Helicobacter pylori. The use of experimental models of gastritis is important to evaluate the biochemical changes and study chemotherapeutic ...

Research paper thumbnail of Association of heat shock protein70-2 genotypes with hypertension among textile workers occupationally exposed to noise

Environmental Science and Pollution Research

Research paper thumbnail of Neurotoxicity of organic solvents with emphasis on the role of iron

Journal of Complementary and Integrative Medicine

Background Exposure to organic solvents (OS) adversely affects different body systems, the centra... more Background Exposure to organic solvents (OS) adversely affects different body systems, the central and peripheral nervous systems being the most susceptible ones. Objectives This study investigated the role of iron in association with some neurotransmitters for diagnosis of neurotoxicity of OS. Methods The study included 90 workers, 50 occupationally exposed to OS and 40 representing control group. Blood samples were collected from the included subjects for determination of serum iron, total iron binding capacity (TIBC), serotonin and gamma-aminobutyric acid (GABA). Results Revealed reduction in serotonin level and serum iron. However, the elevation in GABA and TIBC was observed. The duration of exposure was significantly correlated with iron and serotonin while it was positively correlated with GABA and TIBC. Conclusions Elevated GABA and TIBC with decreased serotonin and serum iron can be used as early diagnostic measures to detect the neurotoxic effects of OS.

Research paper thumbnail of Association of microsomal epoxide hydrolase gene (fast genotype) with lung functions impairment in wood workers

Journal of Complementary and Integrative Medicine

Objectives Exposure to wood dust may lead to impairment of the lung functions. Microsomal epoxide... more Objectives Exposure to wood dust may lead to impairment of the lung functions. Microsomal epoxide hydrolase enzyme (EPHX1) was shown to take part in protection against oxidative stress. An alteration in enzyme activity might be associated with its gene polymorphisms. In vitro polymorphisms in exons 3 (His113Tyr) and 4 (Arg139His) lead to reduced activity (slow allele) and increased activity (fast allele). Macrophage inflammatory protein 2 (MIP-2) is produced in rat lung epithelial cells after exposure to fine particles. We aimed to investigate the associations between mEPHX1 polymorphisms (in exon 3 and 4) and lung function in furniture workers and assessment of MIP-2 effect. Methods Our study was performed on 70 wood dust exposed male workers and 70 matched normal controls subjects. Ventilatory function tests were measured by spirometer, MIP-2 was performed by ELISA methods and EPHX gene was done by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) metho...

Research paper thumbnail of Hepatotoxicity of aflatoxin B1 and its oxidative effects in wood dust Egyptian exposed workers

Archives of Environmental & Occupational Health

Research paper thumbnail of Epigenetic study of global gene methylation in PON1, XRCC1 and GSTs different genotypes in rural and urban pesticide exposed workers

Journal of Complementary and Integrative Medicine

BackgroundEpigenetic represents a study of occurred heritable gene expression changes without cha... more BackgroundEpigenetic represents a study of occurred heritable gene expression changes without changing in the DNA sequence. It includes DNA methylation and miRNA expression that attract increasing attention as potential links between the genetic and environmental determinants of health and disease. Pesticide exposure is associated with adverse health effects and DNA methylation due to oxidative stress induced following its exposure. This study aimed to define the association of genetic polymorphisms of XRCC1, PON1, GSTP1 and GST genes with global genes DNA methylation in urban and rural occupationally pesticides exposed workers.MethodsThis study included 100 pesticides exposed workers; 50 rural sprayers (RE) and 50 urban researchers (UE). Controls included equal numbers. DNA methylation of global genes was evaluated by pyrosequencing assay. XRCC1, PON1 and GSTP1 genotyping were assessed by PCR–RFLP, and GST M1 and T1 were performed by PCR.ResultsThe results of this study revealed th...

Research paper thumbnail of Audiometric notch for the prediction of early occupational hearing loss and its association with the interleukin-1beta genotype

Journal of Taibah University Medical Sciences

Research paper thumbnail of Early prediction of liver carcinogenicity due to occupational exposure to pesticides

Mutation Research/Genetic Toxicology and Environmental Mutagenesis

Research paper thumbnail of Impact of occupational cadmium exposure on bone in sewage workers

International Journal of Occupational and Environmental Health

Research paper thumbnail of Assessment of Anticholinesterase Toxicity, Oxidative Stress and Antioxidant Status in Carbamate and Organophosphorus PesticidesExposed Agricultural Workers

International Journal of Pharmaceutical and Clinical Research

Among the numerous pesticides, anticholinesterase compounds are widely used. Their toxicity induc... more Among the numerous pesticides, anticholinesterase compounds are widely used. Their toxicity induced by cholinesterase inhibition at the synapses and neuromuscular junctions, leading to neurological disorders. Inhibition of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) has been used as sensitive biomarkers for pesticides exposure. In the present study, AChE and BuChE levels were estimated in agricultural workers exposed to carbamate and organophosphorus pesticides with average 9.8±3.5 years relative to the controls. The toxic effects of pesticides may be attributed to induction of oxidative stress and alteration in antioxidant system. Our results showed significant decrease in AChE and BuChE levels with inhibition percentage of 39% and 61% respectively, in exposed workers than controls. Additionally, there was a significant increase in malondialdehyde (MDA) as an oxidative stress marker. Concerning antioxidant status, there was significant decrease in reduced glutathi...

Research paper thumbnail of GSTP1 and XRCC1 polymorphisms and DNA damage in agricultural workers exposed to pesticides

Mutation Research/Genetic Toxicology and Environmental Mutagenesis

Pesticide exposure may be associated with increased risk of genotoxicity and carcinogenesis. Thes... more Pesticide exposure may be associated with increased risk of genotoxicity and carcinogenesis. These risks may be affected by polymorphisms of genes for glutathione transferase-dependent metabolism of pesticides and for DNA repair. We studied the prevalence of GSTP1 and XRCC1 polymorphisms and their possible correlation with DNA damage following prolonged pesticide exposure. DNA damage was estimated by the comet assay in peripheral blood samples from 51 pesticide-exposed workers and 50 controls. GSTP1 (105) and XRCC1 (399 and 194) genotypes were identified by restriction fragment length analysis. Individuals carrying theGSTP1 Ile-Ile or XRCC1399 Arg-Arg genotypes showed greater DNA damage than observed for other alleles.

Research paper thumbnail of Hepatotoxic Effect of Aflatoxin B1 and Polymorphisms in Glutathione S-Transferase in Millers Occupationally Exposed to Aspergillus

Little is known whether occupational exposure to aflatoxin might have a potential hazard. The pre... more Little is known whether occupational exposure to aflatoxin might have a potential hazard. The present work aimed to study the hepatotoxic effects of occupational exposure to AFB1 as a metabolite of high Aspergillus concentrations in the working environments, and the polymorphism of GST gene in exposed workers. The study was performed on 97 flour mill workers exposed to high Aspergillus concentrations and 78 non-exposed controls. The levels of AFB1/Alb (ng/g), AST and ALT of the workers were significantly higher than the controls, while there was no significance difference in the ALP levels between the two groups. The present results revealed that AFB1/Alb levels were significantly higher in the workers with the different GST alleles compared to the control groups. Additionally, AFB1/Alb levels were significantly higher in the workers with GSTT1 compared to the workers with different GST alleles (GSTM1 and Null (GSTT1&GSTM1)) and the controls with Null (GSTT1&GSTM1) alleles. In conclusion, occupational exposure to the high concentrations of Aspergillus in the workplace may cause an increase in the AFB1/Alb and the liver enzymes in flour mill workers. These results also showed that the Null (GSTM1 & GSTT1) alleles are the most common type in the studied population. The workers with GSTT1 have lower ability to detoxify AFB1.

Research paper thumbnail of Gastritis Induced by Helicobacter pylori Infection in Experimental Rats

Digestive Diseases and Sciences, Jan 22, 2010

Background Gastritis, an inflammation of gastric mucosa, may be due to many pathological factors ... more Background Gastritis, an inflammation of gastric mucosa, may be due to many pathological factors and infection, such as with Helicobacter pylori. The use of experimental models of gastritis is important to evaluate the biochemical changes and study chemotherapeutic intervention. In a previous study we demonstrated an acute gastritis model induced by iodoacetamide. Aims Our objective in this study was to evaluate a new gastritis model induced by H. pylori infection in experimental rats in terms of certain biomarkers in serum and mucosal tissues in addition to histopathological examination. Methods Gastritis was induced in 20 albino Wistar rats by H. pylori isolated from antral biopsy taken from a 49-yearold male patient endoscopically diagnosed as having H. pylori infection. Another ten rats were used as controls. Serum gastrin, pepsinogen I activity, interleukin-6 (IL-6) and gastric mucosal myeloperoxidase (MPO) activity and prostaglandin E 2 (PGE 2) were measured. Immunostaining for inducible nitric oxide synthase (iNOS), nitrotyrosine and DNA fragmentation were used to further evaluate H. pylori-induced gastritis. Results Serum gastrin, IL-6, mucosal MPO activity, and PGE 2 demonstrated significant increases joined with a decreased serum pepsinogen I activity (P \ 0.001). Immunohistochemistry demonstrated positive reaction for iNOS, nitrotyrosine and DNA fragmentation. Conclusions Helicobacter pylori-induced gastritis models demonstrated massive oxidative stress and pronounced injury in mucosal tissue. Since our model in rats reflected the clinical picture of H. pylori infection, it can be considered as a consistent model to study chemotherapeutic intervention for this type of gastritis.

Research paper thumbnail of Comparative Hepatotoxicity of Aflatoxin B1 among Workers Exposed to Different Organic Dust with Emphasis on Polymorphism Role of Glutathione S-Transferase Gene

Open Access Macedonian Journal of Medical Sciences, 2016

AIM: The study aimed to investigate effects of organic dust exposure from different sources on af... more AIM: The study aimed to investigate effects of organic dust exposure from different sources on aflatoxin B1-albumin adducts (AFB1/Alb), and role of glutathione S-transferase (GST) gene polymorphism in hepatotoxicity of (AFB1) among exposed workers. MATERIAL AND METHODS: Liver enzymes, AFB1/Alb, and GST polymorphism were estimated in 132 wheat flour dust and 87 woods sawmill workers, and 156 controls.RESULTS: Results revealed that AFB1/Alb and liver enzymes were significantly elevated in exposed workers compared to controls, and were significantly higher in sawmill workers compared to flour workers. AFB1/Alb in flour and sawmill workers with GSTT1 and GSTM1&GSTT1 null genotypes were significantly higher than controls, and in sawmill workers with GSTM1&GSTT1 null than flour workers. Liver enzymes (ALT and AST) in sawmill workers were significantly higher than flour workers and controls in all GST polymorphism; except in GSTT1 polymorphism, where these enzymes were significantly higher...

Research paper thumbnail of Effect of Helicobacter pylori on the mucosa of the lower end of the esophagus in induced chronic gastritis in adult albino rats

The Egyptian Journal of Histology, 2013

Research paper thumbnail of Hepatotoxic Effect of Aflatoxin B1 and Polymorphisms in Glutathione S-Transferase in Millers Occupationally Exposed to Aspergillus

Little is known whether occupational exposure to aflatoxin might have a potential hazard. The pre... more Little is known whether occupational exposure to aflatoxin might have a potential hazard. The present work aimed to study the hepatotoxic effects of occupational exposure to AFB1 as a metabolite of high Aspergillus concentrations in the working environments, and the polymorphism of GST gene in exposed workers. The study was performed on 97 flour mill workers exposed to high Aspergillus concentrations and 78 non-exposed controls. The levels of AFB1/Alb (ng/g), AST and ALT of the workers were significantly higher than the controls, while there was no significance difference in the ALP levels between the two groups. The present results revealed that AFB1/Alb levels were significantly higher in the workers with the different GST alleles compared to the control groups. Additionally, AFB1/Alb levels were significantly higher in the workers with GSTT1 compared to the workers with different GST alleles (GSTM1 and Null (GSTT1&GSTM1)) and the controls with Null (GSTT1&GSTM1) alleles. In concl...

Research paper thumbnail of Carcinogenic effects of aflatoxin B1 among wheat handlers

International Journal of Occupational and Environmental Health, 2014

Epidemiological studies have demonstrated that serum aflatoxin B1 (AFB1) is a hepatocarcinogenic ... more Epidemiological studies have demonstrated that serum aflatoxin B1 (AFB1) is a hepatocarcinogenic mycotoxin and contributor to the high rate of hepatocellular carcinoma (HCC). The prevalence of liver cancer in Egypt is particularly worrisome. In a registry-based analysis of occupational risk for HCC, significant excesses were observed especially for grain mill workers. The aim of this study was to assess the hepatic carcinogenicity of AFB1 in wheat handlers. Serum AFB1/albumin (AFB1/Alb), alpha-fetoprotein (AFP), alpha-l-fucosidase (AFU), and arginase were estimated in exposed wheat handlers including millers and bakers. The control group was composed of non-occupationally exposed workers. AFB1/Alb and AFU were significantly higher among workers employed as bakers compared to mill workers and controls. Mill workers had higher levels of AFB1/Alb than the controls. AFB1/Alb, AFP, and AFU were all significantly higher and arginase was significantly lower among HCC cases compared to the other groups. There was a significant correlation between AFU and AFB1/Alb in bakers and between AFP and AFB1/Alb in HCC cases. Arginase was inversely correlated with AFB1/Alb in HCC cases. AFB1/Alb was significantly correlated with the duration of exposure in bakers. Wheat handlers exposed to Aspergillus flavus have a high risk of elevated serum AFB1/Alb levels and AFU.

Research paper thumbnail of Effect of Nigella sativa oil versus amoxicillin in induced chronic fundic gastritis in adult female albino rats

The Egyptian Journal of Histology, 2011

Advertisement. Close Window. Close Window. Thank you for choosing to subscribe to the eTOC for Eg... more Advertisement. Close Window. Close Window. Thank you for choosing to subscribe to the eTOC for Egyptian Journal of Histology. Enter your Email address: Wolters Kluwer Health may email you for journal alerts and information ...

Research paper thumbnail of Pattern of gastritis as manipulated by current state of Helicobacter pylori infection

wseas.us

Helicobacter pylori (H. pylori) infection prevails from 60-80% in patients with gastric ulcer and... more Helicobacter pylori (H. pylori) infection prevails from 60-80% in patients with gastric ulcer and 90-100% in those having duodenal ulcer. Patients with such type of chronic infection are at increased risk to develop peptic ulcers or gastric adenocarcinomas. The present work aims mainly to identify the pattern of chronic gastritis and potential effect of H. pylori infection using certain biomarkers, histological and immunochemical tests. Fifty eight individuals, clinically diagnosed as having chronic gastritis, were participated in the present study. They were categorized into 2 groups, the first one (31%) demonstrated positive reaction to IgM antibodies of Helicobacter pylori (H. pylori) (>40u/ml) and the second group (69%) demonstrated negative reaction. Blood and antral biopsy samples were collected, directed to determination of serum gastrin, pepsinogen I (PgI), pepsinogen II (PgII), prostaglandin E 2 (PGE 2) and interlukin-6 (IL-6). Immunohistochemistry technique was also done in antral biopsy to demonstrate the expression of inducible nitric oxide synthase (iNOS), nitrotyrosine, DNA fragmentation, myeloperoxidase and histopathological examination. Serum gastrin, PgI, PgII, PGE 2 , IL-6 demonstrated significant increase in gastritis patients as compared to normal group. PgI, PgII showed significant increase joined with slight increase of IL-6 in IgM positive group as compared to negative one. Immunostaining testes in antral biopsy showed strong positive reactions for the above mentioned markers as compared to IgM negative group (mild positive reaction). In conclusion, gastritis patients who express IgM antibodies for H. pylori infection showed higher gastrinaemia and more pronounced atrophic, inflammatory and apoptotic damage than those not expressing IgM antibodies.

Research paper thumbnail of Gastritis Induced by Helicobacter pylori Infection in Experimental Rats

Digestive diseases and …, 2010

Background Gastritis, an inflammation of gastric mucosa, may be due to many pathological factors ... more Background Gastritis, an inflammation of gastric mucosa, may be due to many pathological factors and infection, such as with Helicobacter pylori. The use of experimental models of gastritis is important to evaluate the biochemical changes and study chemotherapeutic ...