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Papers by moyassar zaki

Asian Pacific Journal of Cancer Prevention, Mar 1, 2022

Worldwide, breast cancer (BC) is the most common cancer among women (Ferlay et al., 2015). Accord... more Worldwide, breast cancer (BC) is the most common cancer among women (Ferlay et al., 2015). According to the 2014 published data of the Egyptian National Population Based Cancer Registry Program, breast cancer incidence in Lower, Middle, and Upper Egypt accounted for 33.8%, 26.8% and 38.7% respectively of all cancer types in females (Ibrahim et al., 2014). Metastatic disease remains a significant contributor to morbidity and mortality in patients with breast cancer. An improved molecular and biochemical understanding of the metastatic process is expected to fuel the development of new therapeutic approaches (Tao et al., 2015). Metastasis suppressors, defined by their abilities to inhibit metastasis without blocking tumor growth, are

Asian Pacific Journal of Cancer Prevention, Jul 1, 2020

Background and aim of the work: Helicobacter pylori-associated gastric ulcer (H.pylori-GU) is a s... more Background and aim of the work: Helicobacter pylori-associated gastric ulcer (H.pylori-GU) is a serious condition, not only because H.pylori is identified as a grade 1 carcinogen but also because GU is a precancerous condition. Identification and treatment of H.pylori-GU may prevent the sequential progression of dysplasia to carcinoma. Trefoil factor 3 (Tf3) has been implicated in gastric mucosal repair. We compared serum Tf3 to gastric endoscopy in diagnosing H.pylori-GU. Subjects and methods: The study included eighty patients suffering from H.pylori induced gastritis, forty of which presented with GU. Gastric endoscopy with slide urease test was used to diagnose H.pylori-GU. Serum Tf3 level was determined using an enzyme immunoassay in all patients as well as thirty healthy volunteers. Results: Serum Tf3 showed a significant stepwise decrease among the studied groups. It was significantly lower in patients compared to the control group (p<0.001). Furthermore, it was lower in those with GU compared to those without GU (p=0.023). Based on a receiver operating characteristic curve generated cut off value of 2.4 ng/mL, the diagnostic performance of serum Tf3 as a biomarker of H.pylori-GU revealed a diagnostic specificity of 42.5%, sensitivity of 67.5%, positive and negative predictive values of 54% and 56.67% respectively. Conclusion: Although serum Tf3 showed significant variation in H.pylori-GU, further studies are warranted to confirm its role in the pathogenesis of gastric ulcers.

Journal of diabetes mellitus, Feb 1, 2016

Objectives: Diabetic retinopathy (DR) is one of the most common microvascular complications of ty... more Objectives: Diabetic retinopathy (DR) is one of the most common microvascular complications of type 2 diabetes mellitus (T2DM). It is multifactorial with the contribution of multiple genetic factors. We questioned the association of polymorphisms in the peroxisome proliferator-activated receptor ɣ2 (PPARɣ2) gene (Pro12Ala and C161T) with DR in an Egyptian population. Methods: This case control study included one hundred healthy individuals and 252 T2DM among them 122 with DR and 130 without DR. Genotyping was done by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Results: The Pro12Ala Ala allele was associated with decreased risk of DR with an odds ratio (OR) of 0.484, 95% confidence interval (CI) (0.254-0.920), and a p value = 0.024. The C161T T allele was associated with increased risk of DR with OR = 2.593, 95% CI (1.672-4.020), p < 0.001. However, when considering other covariates such as glycosylated hemoglobin (HbA1c) in multivariate regression analysis only C161T was associated with increased risk of DR with OR = 3.479, 95% CI (1.907-6.346), p < 0.001, while the significant association with Pro12Ala was lost. HbA 1c was higher in Pro/Pro genotype when compared to those with

Genetic Testing and Molecular Biomarkers, Jul 1, 2015

Epigenetic changes, including DNA methylation, are recognized as one of the potential mechanisms ... more Epigenetic changes, including DNA methylation, are recognized as one of the potential mechanisms involved in the pathogenesis of hepatocellular carcinoma (HCC). We aimed to study the methylation status of the promoter region of Serine peptidase inhibitor/hepatocyte growth factor activator inhibitor type 2 (SPINT2/HAI-2) tumor suppressor gene in hepatitis C virus (HCV)-infected cirrhotic patients with and without HCC. Methyl-specific polymerase (MSP) chain reaction was used to detect CpG methylation of the SPINT2/HAI-2 gene promoter in peripheral blood samples of 30 HCC and 50 HCV cirrhotic cases, along with 50 normal individuals. Aberrant methylation showed a stepwise increase in frequency from 40% in controls to 64% in HCV cirrhotics, and 66.7% in HCC cases with a significant difference among the studied groups (p=0.021). The combined patient groups had an increased risk of aberrant methylation with an odds ratio (OR) of 2.52, a 95% confidence interval (CI) of 1.23-5.14, and a p-value of 0.05 that became more statistically significant after adjusting for age (OR=2.4, 95% CI=1.13-5.26, p-value=0.012), thereby confirming the association between HCV infection and aberrant methylation. Our study highlights the role of promoter hypermethylation in the multistep process of hepatocarcinogenesis, providing potential clinical applications in diagnosis and prognosis.

Pregnancy Hypertension, 2012

Objective: One of the theories involved in the pathogenesis of pregnancy induced hypertension inv... more Objective: One of the theories involved in the pathogenesis of pregnancy induced hypertension involves salt and water retention. We aimed to measure the proenzyme convertase corin, responsible for pro-atrial natriuretic peptide (ANP) cleavage to active ANP, in plasma of hypertensive pregnant females. Study design: Sixty pregnant females suffering from pregnancy induced hypertension in second and third trimesters of pregnancy were compared to twenty eight healthy pregnant females of the same gestational period. Concomitant urine and plasma samples were collected for the determination of some biochemical parameters. Plasma soluble corin and N-terminal (NT) pro-ANP (1-98) values were determined in both groups using enzyme immunoassays. Results: Plasma soluble corin mean value was significantly higher in the patient group compared to the control group. Upon dividing the patient group according to blood pressure, plasma NT pro-ANP showed significantly higher mean value in the group with blood pressure P 140/90 mmHg compared to the group with blood pressure < 140/90 mmHg and control group. Conclusions: High plasma soluble corin and NT pro-ANP values in hypertensive pregnant females particularly those with blood pressure P 140/90 mmHg speculates an ANP receptor/ post receptor signaling defect, which would aggravate the pregnancy induced hypertensive state.

Asian Pacific Journal of Cancer Prevention, Mar 1, 2022

Worldwide, breast cancer (BC) is the most common cancer among women (Ferlay et al., 2015). Accord... more Worldwide, breast cancer (BC) is the most common cancer among women (Ferlay et al., 2015). According to the 2014 published data of the Egyptian National Population Based Cancer Registry Program, breast cancer incidence in Lower, Middle, and Upper Egypt accounted for 33.8%, 26.8% and 38.7% respectively of all cancer types in females (Ibrahim et al., 2014). Metastatic disease remains a significant contributor to morbidity and mortality in patients with breast cancer. An improved molecular and biochemical understanding of the metastatic process is expected to fuel the development of new therapeutic approaches (Tao et al., 2015). Metastasis suppressors, defined by their abilities to inhibit metastasis without blocking tumor growth, are

Asian Pacific Journal of Cancer Prevention, 2020

Background and aim of the work: Helicobacter pylori-associated gastric ulcer (H.pylori-GU) is a s... more Background and aim of the work: Helicobacter pylori-associated gastric ulcer (H.pylori-GU) is a serious condition, not only because H.pylori is identified as a grade 1 carcinogen but also because GU is a precancerous condition. Identification and treatment of H.pylori-GU may prevent the sequential progression of dysplasia to carcinoma. Trefoil factor 3 (Tf3) has been implicated in gastric mucosal repair. We compared serum Tf3 to gastric endoscopy in diagnosing H.pylori-GU. Subjects and methods: The study included eighty patients suffering from H.pylori induced gastritis, forty of which presented with GU. Gastric endoscopy with slide urease test was used to diagnose H.pylori-GU. Serum Tf3 level was determined using an enzyme immunoassay in all patients as well as thirty healthy volunteers. Results: Serum Tf3 showed a significant stepwise decrease among the studied groups. It was significantly lower in patients compared to the control group (p<0.001). Furthermore, it was lower in those with GU compared to those without GU (p=0.023). Based on a receiver operating characteristic curve generated cut off value of 2.4 ng/mL, the diagnostic performance of serum Tf3 as a biomarker of H.pylori-GU revealed a diagnostic specificity of 42.5%, sensitivity of 67.5%, positive and negative predictive values of 54% and 56.67% respectively. Conclusion: Although serum Tf3 showed significant variation in H.pylori-GU, further studies are warranted to confirm its role in the pathogenesis of gastric ulcers.

Alexandria Journal of Medicine, 2019

El-Banna (2019) Serum sclerostin as a potential biomarker of vascular and valvular types of calci... more El-Banna (2019) Serum sclerostin as a potential biomarker of vascular and valvular types of calcification in chronic kidney disease cases with and without maintenance hemodialysis,

American Journal of Molecular Biology, 2016

Objectives: Diabetic nephropathy (DN) is one of the chronic microangiopathic complications of typ... more Objectives: Diabetic nephropathy (DN) is one of the chronic microangiopathic complications of type 2 diabetes (T2DM) and has become the most frequent cause of end-stage renal disease. The XbaI polymorphism in the glucose transporter (GLUT1) has been suggested in the development of DN. We examined the association between XbaI polymorphism of GLUT1 and susceptibility to T2DM and development of DN. Methods: The study included 227 T2DM patients divided into 107 without DN (DM − DN) and 120 with DN (DM + DN), in addition to 100 apparently healthy controls. Genotyping was done by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Results: The GLUT1 XbaI T allele was associated with increased susceptibility to T2DM, when comparing the healthy controls to the whole diabetic group, odds ratio (OR) = 1.899, 95% confidence interval (CI) (1.149-3.136), p = 0.011. This association was also significant between healthy controls and DM − DN OR = 1.997 (1.079-3.699), p = 0.026 as well as between healthy controls and DM + DN OR = 1.818 (1.016-3.253), p = 0.042. However there was no significant association of XbaI polymorphism with DN when comparing DM − DN to DM + DN OR = 0.910 (0.474-1.747), p = 0.777. Conclusion: XbaI T allele is associated with increased susceptibility to T2DM, but not to development of DN. Further studies are needed to replicate such findings.

Genetic Testing and Molecular Biomarkers, 2015

Epigenetic changes, including DNA methylation, are recognized as one of the potential mechanisms ... more Epigenetic changes, including DNA methylation, are recognized as one of the potential mechanisms involved in the pathogenesis of hepatocellular carcinoma (HCC). We aimed to study the methylation status of the promoter region of Serine peptidase inhibitor/hepatocyte growth factor activator inhibitor type 2 (SPINT2/HAI-2) tumor suppressor gene in hepatitis C virus (HCV)-infected cirrhotic patients with and without HCC. Methyl-specific polymerase (MSP) chain reaction was used to detect CpG methylation of the SPINT2/HAI-2 gene promoter in peripheral blood samples of 30 HCC and 50 HCV cirrhotic cases, along with 50 normal individuals. Aberrant methylation showed a stepwise increase in frequency from 40% in controls to 64% in HCV cirrhotics, and 66.7% in HCC cases with a significant difference among the studied groups (p=0.021). The combined patient groups had an increased risk of aberrant methylation with an odds ratio (OR) of 2.52, a 95% confidence interval (CI) of 1.23-5.14, and a p-value of 0.05 that became more statistically significant after adjusting for age (OR=2.4, 95% CI=1.13-5.26, p-value=0.012), thereby confirming the association between HCV infection and aberrant methylation. Our study highlights the role of promoter hypermethylation in the multistep process of hepatocarcinogenesis, providing potential clinical applications in diagnosis and prognosis.

Genetic Testing and Molecular Biomarkers, 2015

Background: Biopsies obtained from lung cancers contain a mixture of cancerous and healthy tissue... more Background: Biopsies obtained from lung cancers contain a mixture of cancerous and healthy tissues. The mutant-enriched polymerase chain reaction (ME-PCR) identifies low-level somatic DNA mutations within an excess wild-type sample. Aims: This study aimed at comparing nonenriched PCR (NE-PCR) versus ME-PCR for the detection of two epidermal growth factor receptor (EGFR) gene mutations among nonsmall cell lung cancer patients. Methods: Fifty lung tissue biopsies were screened for inframe TTAA deletions in exon-19 and the L858R point mutation in exon-21, using ME-PCR and NE-PCR, followed by capillary electrophoresis. Results: Only exon-19 deletions were detected in 22% and 18% of cases using ME-PCR and NE-PCR, respectively. Diagnostic performance of the NE-PCR versus the ME-PCR serving as a ''gold standard'' revealed a sensitivity of 82%, and a specificity of 100%, with positive and negative predictive values of 100% and 95%, respectively, and an overall accuracy of 96%. Despite a strong agreement shown between the two assays (K = 0.875), the NE-PCR showed an 18% false-negative rate in bronchoscopically obtained biopsies compared to ME-PCR. Conclusion: The false negativity encountered with NE-PCR in bronchoscopically obtained samples makes ME-PCR the technique of choice in such situations.

Pregnancy Hypertension: An International Journal of Women's Cardiovascular Health, 2012

Objective: One of the theories involved in the pathogenesis of pregnancy induced hypertension inv... more Objective: One of the theories involved in the pathogenesis of pregnancy induced hypertension involves salt and water retention. We aimed to measure the proenzyme convertase corin, responsible for pro-atrial natriuretic peptide (ANP) cleavage to active ANP, in plasma of hypertensive pregnant females. Study design: Sixty pregnant females suffering from pregnancy induced hypertension in second and third trimesters of pregnancy were compared to twenty eight healthy pregnant females of the same gestational period. Concomitant urine and plasma samples were collected for the determination of some biochemical parameters. Plasma soluble corin and N-terminal (NT) pro-ANP (1-98) values were determined in both groups using enzyme immunoassays. Results: Plasma soluble corin mean value was significantly higher in the patient group compared to the control group. Upon dividing the patient group according to blood pressure, plasma NT pro-ANP showed significantly higher mean value in the group with blood pressure P 140/90 mmHg compared to the group with blood pressure < 140/90 mmHg and control group. Conclusions: High plasma soluble corin and NT pro-ANP values in hypertensive pregnant females particularly those with blood pressure P 140/90 mmHg speculates an ANP receptor/ post receptor signaling defect, which would aggravate the pregnancy induced hypertensive state.

Molecular Biology Reports, 2012

The researches attempting to associate the PPARc C161T polymorphism with coronary artery disease ... more The researches attempting to associate the PPARc C161T polymorphism with coronary artery disease (CAD) yielded complicated and contradictory results. We aimed for more precise estimate of the relationship and conducted a comprehensive meta-analysis. Publications written in English or Chinese were screened in MEDLINE, Embase, CNKI, Wanfang and CBM. Data on 11 studies including 3,020 cases and 2,853 controls were extracted. A random-effects model was available to synthesize the inconsistent outcomes of the individual studies, while addressing between-study heterogeneity and publication bias. The PPARc C161T polymorphism followed Hard-Weinberg Equilibrium for all studies (P [ 0.05).Overall, there was no evidence for a significant association under all genetic models but with distinct heterogeneity (T vs. C: P = 0.29, OR = 0.91, 95 %CI 0.77-1.08, P heterogeneity = 0.004, I 2 = 61.2 %). However, in the subgroup analysis by ethnicity, the T allele carriers showed a prominent 26 % risk reduction of CAD among Chinese (dominant genetic model: P = 0.03, 95 %CI 0.57-0.97, P heterogeneity = 0.03, I 2 = 56.1 %). After dividing into population source, the significance of CAD risk reduction was strengthened in hospital-based studies (allele comparison: P = 0.04, OR = 0.82, 95 %CI 0.67-1.00, P heterogeneity = 0.04, I 2 = 52.5 %; dominant model: P = 0.01, OR = 0.73, 95 %CI 0.57-0.92, P heterogeneity = 0.05, I 2 = 50.8 %). There was no obvious publication bias verified in the method of funnel plot and Egger's linear regression test (t =-0.11, P = 0.913). Taken together, our results revealed the PPARc C161T polymorphism might play a moderate protective effect on developing CAD among Chinese, but not among Caucasians.

CHEST Journal, 2007

PURPOSE: Background The first step in the evaluation of patients with pleural effusion is todeter... more PURPOSE: Background The first step in the evaluation of patients with pleural effusion is todetermine whether the effusion is a transudate or an exudate. Osteopontin (OPN) isa pleiotropic integrin-binding protein with many functions. We assessed pleuraleffusion and serum concentrations of OPN and C reactive protein (CRP) in patientswith different types of pleural effusions. METHODS: Methods The current study comprised three groups: 20 patients with transudativeeffusion, 30 patients with malignant effusion and 30 patients with tuberculouseffusion. OPN was analysed using commercially available enzyme-linkedimmunosorbent assay kit. RESULTS: Results OPN effusion values were significantly higher in exudates (bothmalignant and tuberculous effusion cases) compared with transudative effusion.Also when compared separately, patients with tuberculous effusion and those withmalignant effusion had a significantly higher fluid and OPN effusion/serum ratiothan those with transudative effusion. Patient with tuberculous effusion had asignificantly higher serum CRP effusion and effusion/serum ratio of CRP than thosewith malignant or transudative effusion. CONCLUSION: Conclusion OPN is significantly increased in exudative effusion compared withtransudative ones. However, serum OPN and effusion/serum OPN ratio were notsignificantly different in patients with malignant and those with tuberculouseffusions. The lack of difference in serum OPN and effusion/serum OPN ratiobetween patients with malignant and those with tuberculous effusion may beattributed to the heterogeneity of the malignant effusion group. Receiver-operatingcurves analysis has shown that effusion/serum CRP ratio outperformed effusion/serum OPN ratio as a diagnostic marker for tuberculous pleural effusion. CLINICAL IMPLICATIONS: Elevated OPN level may suggest that the effusion is a seconadry to Tuberculous pleursy. However, CRP offer a better diagnostic performance than OPN.

Background The first step in the evaluation of patients with pleural effusion is to determine whe... more Background The first step in the evaluation of patients with pleural effusion is to determine whether the effusion is a transudate or an exudate. Osteopontin (OPN) is a pleiotropic integrin-binding protein with many functions. We assessed pleural effusion and serum concentrations of OPN and C-reactive protein (CRP) in patients with different types of pleural effusions. Methods The current study comprised three groups: 20 patients with transudative effusion, 30 patients with malignant effusion and 30 patients with tuberculous effusion. OPN was analysed using a commercially available enzyme-linked immunosorbent assay kit. Results OPN effusion values were significantly higher in exudates (both malignant and tuberculous effusion cases) compared with transudative effusion. Also when compared separately, patients with tuberculous effusion and those with malignant effusion had a significantly higher fluid and OPN effusion/serum ratio than those with transudative effusion. Patients with tuberculous effusion had a significantly higher serum CRP effusion and effusion/serum ratio of CRP than those with malignant or transudative effusion. Conclusion OPN is significantly increased in exudative effusion compared with transudative ones. However, serum OPN and effusion/serum OPN ratio were not significantly different in patients with malignant from those with tuberculous effusions. The lack of difference in serum OPN and effusion/serum OPN ratio between patients with malignant and those with tuberculous effusion may be attributed to the heterogeneity of the malignant effusion group. Receiver-operating characteristic (ROC) curve analysis has shown that effusion/serum CRP ratio outperformed effusion/serum OPN ratio as a diagnostic marker for tuberculous pleural effusion.

American Journal of Otolaryngology, 2004

PURPOSE: To investigate the influence of the glutathione S-transferase (GST) M1, T1, and P1 genot... more PURPOSE: To investigate the influence of the glutathione S-transferase (GST) M1, T1, and P1 genotypes on the laryngeal squamous cell carcinoma risk. MATERIAL AND METHODS: The study group consisted of 42 white patients with laryngeal squamous cell carcinoma ( ...

Asian Pacific Journal of Cancer Prevention, Mar 1, 2022

Worldwide, breast cancer (BC) is the most common cancer among women (Ferlay et al., 2015). Accord... more Worldwide, breast cancer (BC) is the most common cancer among women (Ferlay et al., 2015). According to the 2014 published data of the Egyptian National Population Based Cancer Registry Program, breast cancer incidence in Lower, Middle, and Upper Egypt accounted for 33.8%, 26.8% and 38.7% respectively of all cancer types in females (Ibrahim et al., 2014). Metastatic disease remains a significant contributor to morbidity and mortality in patients with breast cancer. An improved molecular and biochemical understanding of the metastatic process is expected to fuel the development of new therapeutic approaches (Tao et al., 2015). Metastasis suppressors, defined by their abilities to inhibit metastasis without blocking tumor growth, are

Asian Pacific Journal of Cancer Prevention, Jul 1, 2020

Background and aim of the work: Helicobacter pylori-associated gastric ulcer (H.pylori-GU) is a s... more Background and aim of the work: Helicobacter pylori-associated gastric ulcer (H.pylori-GU) is a serious condition, not only because H.pylori is identified as a grade 1 carcinogen but also because GU is a precancerous condition. Identification and treatment of H.pylori-GU may prevent the sequential progression of dysplasia to carcinoma. Trefoil factor 3 (Tf3) has been implicated in gastric mucosal repair. We compared serum Tf3 to gastric endoscopy in diagnosing H.pylori-GU. Subjects and methods: The study included eighty patients suffering from H.pylori induced gastritis, forty of which presented with GU. Gastric endoscopy with slide urease test was used to diagnose H.pylori-GU. Serum Tf3 level was determined using an enzyme immunoassay in all patients as well as thirty healthy volunteers. Results: Serum Tf3 showed a significant stepwise decrease among the studied groups. It was significantly lower in patients compared to the control group (p<0.001). Furthermore, it was lower in those with GU compared to those without GU (p=0.023). Based on a receiver operating characteristic curve generated cut off value of 2.4 ng/mL, the diagnostic performance of serum Tf3 as a biomarker of H.pylori-GU revealed a diagnostic specificity of 42.5%, sensitivity of 67.5%, positive and negative predictive values of 54% and 56.67% respectively. Conclusion: Although serum Tf3 showed significant variation in H.pylori-GU, further studies are warranted to confirm its role in the pathogenesis of gastric ulcers.

Journal of diabetes mellitus, Feb 1, 2016

Objectives: Diabetic retinopathy (DR) is one of the most common microvascular complications of ty... more Objectives: Diabetic retinopathy (DR) is one of the most common microvascular complications of type 2 diabetes mellitus (T2DM). It is multifactorial with the contribution of multiple genetic factors. We questioned the association of polymorphisms in the peroxisome proliferator-activated receptor ɣ2 (PPARɣ2) gene (Pro12Ala and C161T) with DR in an Egyptian population. Methods: This case control study included one hundred healthy individuals and 252 T2DM among them 122 with DR and 130 without DR. Genotyping was done by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Results: The Pro12Ala Ala allele was associated with decreased risk of DR with an odds ratio (OR) of 0.484, 95% confidence interval (CI) (0.254-0.920), and a p value = 0.024. The C161T T allele was associated with increased risk of DR with OR = 2.593, 95% CI (1.672-4.020), p < 0.001. However, when considering other covariates such as glycosylated hemoglobin (HbA1c) in multivariate regression analysis only C161T was associated with increased risk of DR with OR = 3.479, 95% CI (1.907-6.346), p < 0.001, while the significant association with Pro12Ala was lost. HbA 1c was higher in Pro/Pro genotype when compared to those with

Genetic Testing and Molecular Biomarkers, Jul 1, 2015

Epigenetic changes, including DNA methylation, are recognized as one of the potential mechanisms ... more Epigenetic changes, including DNA methylation, are recognized as one of the potential mechanisms involved in the pathogenesis of hepatocellular carcinoma (HCC). We aimed to study the methylation status of the promoter region of Serine peptidase inhibitor/hepatocyte growth factor activator inhibitor type 2 (SPINT2/HAI-2) tumor suppressor gene in hepatitis C virus (HCV)-infected cirrhotic patients with and without HCC. Methyl-specific polymerase (MSP) chain reaction was used to detect CpG methylation of the SPINT2/HAI-2 gene promoter in peripheral blood samples of 30 HCC and 50 HCV cirrhotic cases, along with 50 normal individuals. Aberrant methylation showed a stepwise increase in frequency from 40% in controls to 64% in HCV cirrhotics, and 66.7% in HCC cases with a significant difference among the studied groups (p=0.021). The combined patient groups had an increased risk of aberrant methylation with an odds ratio (OR) of 2.52, a 95% confidence interval (CI) of 1.23-5.14, and a p-value of 0.05 that became more statistically significant after adjusting for age (OR=2.4, 95% CI=1.13-5.26, p-value=0.012), thereby confirming the association between HCV infection and aberrant methylation. Our study highlights the role of promoter hypermethylation in the multistep process of hepatocarcinogenesis, providing potential clinical applications in diagnosis and prognosis.

Pregnancy Hypertension, 2012

Objective: One of the theories involved in the pathogenesis of pregnancy induced hypertension inv... more Objective: One of the theories involved in the pathogenesis of pregnancy induced hypertension involves salt and water retention. We aimed to measure the proenzyme convertase corin, responsible for pro-atrial natriuretic peptide (ANP) cleavage to active ANP, in plasma of hypertensive pregnant females. Study design: Sixty pregnant females suffering from pregnancy induced hypertension in second and third trimesters of pregnancy were compared to twenty eight healthy pregnant females of the same gestational period. Concomitant urine and plasma samples were collected for the determination of some biochemical parameters. Plasma soluble corin and N-terminal (NT) pro-ANP (1-98) values were determined in both groups using enzyme immunoassays. Results: Plasma soluble corin mean value was significantly higher in the patient group compared to the control group. Upon dividing the patient group according to blood pressure, plasma NT pro-ANP showed significantly higher mean value in the group with blood pressure P 140/90 mmHg compared to the group with blood pressure < 140/90 mmHg and control group. Conclusions: High plasma soluble corin and NT pro-ANP values in hypertensive pregnant females particularly those with blood pressure P 140/90 mmHg speculates an ANP receptor/ post receptor signaling defect, which would aggravate the pregnancy induced hypertensive state.

Asian Pacific Journal of Cancer Prevention, Mar 1, 2022

Worldwide, breast cancer (BC) is the most common cancer among women (Ferlay et al., 2015). Accord... more Worldwide, breast cancer (BC) is the most common cancer among women (Ferlay et al., 2015). According to the 2014 published data of the Egyptian National Population Based Cancer Registry Program, breast cancer incidence in Lower, Middle, and Upper Egypt accounted for 33.8%, 26.8% and 38.7% respectively of all cancer types in females (Ibrahim et al., 2014). Metastatic disease remains a significant contributor to morbidity and mortality in patients with breast cancer. An improved molecular and biochemical understanding of the metastatic process is expected to fuel the development of new therapeutic approaches (Tao et al., 2015). Metastasis suppressors, defined by their abilities to inhibit metastasis without blocking tumor growth, are

Asian Pacific Journal of Cancer Prevention, 2020

Background and aim of the work: Helicobacter pylori-associated gastric ulcer (H.pylori-GU) is a s... more Background and aim of the work: Helicobacter pylori-associated gastric ulcer (H.pylori-GU) is a serious condition, not only because H.pylori is identified as a grade 1 carcinogen but also because GU is a precancerous condition. Identification and treatment of H.pylori-GU may prevent the sequential progression of dysplasia to carcinoma. Trefoil factor 3 (Tf3) has been implicated in gastric mucosal repair. We compared serum Tf3 to gastric endoscopy in diagnosing H.pylori-GU. Subjects and methods: The study included eighty patients suffering from H.pylori induced gastritis, forty of which presented with GU. Gastric endoscopy with slide urease test was used to diagnose H.pylori-GU. Serum Tf3 level was determined using an enzyme immunoassay in all patients as well as thirty healthy volunteers. Results: Serum Tf3 showed a significant stepwise decrease among the studied groups. It was significantly lower in patients compared to the control group (p<0.001). Furthermore, it was lower in those with GU compared to those without GU (p=0.023). Based on a receiver operating characteristic curve generated cut off value of 2.4 ng/mL, the diagnostic performance of serum Tf3 as a biomarker of H.pylori-GU revealed a diagnostic specificity of 42.5%, sensitivity of 67.5%, positive and negative predictive values of 54% and 56.67% respectively. Conclusion: Although serum Tf3 showed significant variation in H.pylori-GU, further studies are warranted to confirm its role in the pathogenesis of gastric ulcers.

Alexandria Journal of Medicine, 2019

El-Banna (2019) Serum sclerostin as a potential biomarker of vascular and valvular types of calci... more El-Banna (2019) Serum sclerostin as a potential biomarker of vascular and valvular types of calcification in chronic kidney disease cases with and without maintenance hemodialysis,

American Journal of Molecular Biology, 2016

Objectives: Diabetic nephropathy (DN) is one of the chronic microangiopathic complications of typ... more Objectives: Diabetic nephropathy (DN) is one of the chronic microangiopathic complications of type 2 diabetes (T2DM) and has become the most frequent cause of end-stage renal disease. The XbaI polymorphism in the glucose transporter (GLUT1) has been suggested in the development of DN. We examined the association between XbaI polymorphism of GLUT1 and susceptibility to T2DM and development of DN. Methods: The study included 227 T2DM patients divided into 107 without DN (DM − DN) and 120 with DN (DM + DN), in addition to 100 apparently healthy controls. Genotyping was done by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Results: The GLUT1 XbaI T allele was associated with increased susceptibility to T2DM, when comparing the healthy controls to the whole diabetic group, odds ratio (OR) = 1.899, 95% confidence interval (CI) (1.149-3.136), p = 0.011. This association was also significant between healthy controls and DM − DN OR = 1.997 (1.079-3.699), p = 0.026 as well as between healthy controls and DM + DN OR = 1.818 (1.016-3.253), p = 0.042. However there was no significant association of XbaI polymorphism with DN when comparing DM − DN to DM + DN OR = 0.910 (0.474-1.747), p = 0.777. Conclusion: XbaI T allele is associated with increased susceptibility to T2DM, but not to development of DN. Further studies are needed to replicate such findings.

Genetic Testing and Molecular Biomarkers, 2015

Epigenetic changes, including DNA methylation, are recognized as one of the potential mechanisms ... more Epigenetic changes, including DNA methylation, are recognized as one of the potential mechanisms involved in the pathogenesis of hepatocellular carcinoma (HCC). We aimed to study the methylation status of the promoter region of Serine peptidase inhibitor/hepatocyte growth factor activator inhibitor type 2 (SPINT2/HAI-2) tumor suppressor gene in hepatitis C virus (HCV)-infected cirrhotic patients with and without HCC. Methyl-specific polymerase (MSP) chain reaction was used to detect CpG methylation of the SPINT2/HAI-2 gene promoter in peripheral blood samples of 30 HCC and 50 HCV cirrhotic cases, along with 50 normal individuals. Aberrant methylation showed a stepwise increase in frequency from 40% in controls to 64% in HCV cirrhotics, and 66.7% in HCC cases with a significant difference among the studied groups (p=0.021). The combined patient groups had an increased risk of aberrant methylation with an odds ratio (OR) of 2.52, a 95% confidence interval (CI) of 1.23-5.14, and a p-value of 0.05 that became more statistically significant after adjusting for age (OR=2.4, 95% CI=1.13-5.26, p-value=0.012), thereby confirming the association between HCV infection and aberrant methylation. Our study highlights the role of promoter hypermethylation in the multistep process of hepatocarcinogenesis, providing potential clinical applications in diagnosis and prognosis.

Genetic Testing and Molecular Biomarkers, 2015

Background: Biopsies obtained from lung cancers contain a mixture of cancerous and healthy tissue... more Background: Biopsies obtained from lung cancers contain a mixture of cancerous and healthy tissues. The mutant-enriched polymerase chain reaction (ME-PCR) identifies low-level somatic DNA mutations within an excess wild-type sample. Aims: This study aimed at comparing nonenriched PCR (NE-PCR) versus ME-PCR for the detection of two epidermal growth factor receptor (EGFR) gene mutations among nonsmall cell lung cancer patients. Methods: Fifty lung tissue biopsies were screened for inframe TTAA deletions in exon-19 and the L858R point mutation in exon-21, using ME-PCR and NE-PCR, followed by capillary electrophoresis. Results: Only exon-19 deletions were detected in 22% and 18% of cases using ME-PCR and NE-PCR, respectively. Diagnostic performance of the NE-PCR versus the ME-PCR serving as a ''gold standard'' revealed a sensitivity of 82%, and a specificity of 100%, with positive and negative predictive values of 100% and 95%, respectively, and an overall accuracy of 96%. Despite a strong agreement shown between the two assays (K = 0.875), the NE-PCR showed an 18% false-negative rate in bronchoscopically obtained biopsies compared to ME-PCR. Conclusion: The false negativity encountered with NE-PCR in bronchoscopically obtained samples makes ME-PCR the technique of choice in such situations.

Pregnancy Hypertension: An International Journal of Women's Cardiovascular Health, 2012

Objective: One of the theories involved in the pathogenesis of pregnancy induced hypertension inv... more Objective: One of the theories involved in the pathogenesis of pregnancy induced hypertension involves salt and water retention. We aimed to measure the proenzyme convertase corin, responsible for pro-atrial natriuretic peptide (ANP) cleavage to active ANP, in plasma of hypertensive pregnant females. Study design: Sixty pregnant females suffering from pregnancy induced hypertension in second and third trimesters of pregnancy were compared to twenty eight healthy pregnant females of the same gestational period. Concomitant urine and plasma samples were collected for the determination of some biochemical parameters. Plasma soluble corin and N-terminal (NT) pro-ANP (1-98) values were determined in both groups using enzyme immunoassays. Results: Plasma soluble corin mean value was significantly higher in the patient group compared to the control group. Upon dividing the patient group according to blood pressure, plasma NT pro-ANP showed significantly higher mean value in the group with blood pressure P 140/90 mmHg compared to the group with blood pressure < 140/90 mmHg and control group. Conclusions: High plasma soluble corin and NT pro-ANP values in hypertensive pregnant females particularly those with blood pressure P 140/90 mmHg speculates an ANP receptor/ post receptor signaling defect, which would aggravate the pregnancy induced hypertensive state.

Molecular Biology Reports, 2012

The researches attempting to associate the PPARc C161T polymorphism with coronary artery disease ... more The researches attempting to associate the PPARc C161T polymorphism with coronary artery disease (CAD) yielded complicated and contradictory results. We aimed for more precise estimate of the relationship and conducted a comprehensive meta-analysis. Publications written in English or Chinese were screened in MEDLINE, Embase, CNKI, Wanfang and CBM. Data on 11 studies including 3,020 cases and 2,853 controls were extracted. A random-effects model was available to synthesize the inconsistent outcomes of the individual studies, while addressing between-study heterogeneity and publication bias. The PPARc C161T polymorphism followed Hard-Weinberg Equilibrium for all studies (P [ 0.05).Overall, there was no evidence for a significant association under all genetic models but with distinct heterogeneity (T vs. C: P = 0.29, OR = 0.91, 95 %CI 0.77-1.08, P heterogeneity = 0.004, I 2 = 61.2 %). However, in the subgroup analysis by ethnicity, the T allele carriers showed a prominent 26 % risk reduction of CAD among Chinese (dominant genetic model: P = 0.03, 95 %CI 0.57-0.97, P heterogeneity = 0.03, I 2 = 56.1 %). After dividing into population source, the significance of CAD risk reduction was strengthened in hospital-based studies (allele comparison: P = 0.04, OR = 0.82, 95 %CI 0.67-1.00, P heterogeneity = 0.04, I 2 = 52.5 %; dominant model: P = 0.01, OR = 0.73, 95 %CI 0.57-0.92, P heterogeneity = 0.05, I 2 = 50.8 %). There was no obvious publication bias verified in the method of funnel plot and Egger's linear regression test (t =-0.11, P = 0.913). Taken together, our results revealed the PPARc C161T polymorphism might play a moderate protective effect on developing CAD among Chinese, but not among Caucasians.

CHEST Journal, 2007

PURPOSE: Background The first step in the evaluation of patients with pleural effusion is todeter... more PURPOSE: Background The first step in the evaluation of patients with pleural effusion is todetermine whether the effusion is a transudate or an exudate. Osteopontin (OPN) isa pleiotropic integrin-binding protein with many functions. We assessed pleuraleffusion and serum concentrations of OPN and C reactive protein (CRP) in patientswith different types of pleural effusions. METHODS: Methods The current study comprised three groups: 20 patients with transudativeeffusion, 30 patients with malignant effusion and 30 patients with tuberculouseffusion. OPN was analysed using commercially available enzyme-linkedimmunosorbent assay kit. RESULTS: Results OPN effusion values were significantly higher in exudates (bothmalignant and tuberculous effusion cases) compared with transudative effusion.Also when compared separately, patients with tuberculous effusion and those withmalignant effusion had a significantly higher fluid and OPN effusion/serum ratiothan those with transudative effusion. Patient with tuberculous effusion had asignificantly higher serum CRP effusion and effusion/serum ratio of CRP than thosewith malignant or transudative effusion. CONCLUSION: Conclusion OPN is significantly increased in exudative effusion compared withtransudative ones. However, serum OPN and effusion/serum OPN ratio were notsignificantly different in patients with malignant and those with tuberculouseffusions. The lack of difference in serum OPN and effusion/serum OPN ratiobetween patients with malignant and those with tuberculous effusion may beattributed to the heterogeneity of the malignant effusion group. Receiver-operatingcurves analysis has shown that effusion/serum CRP ratio outperformed effusion/serum OPN ratio as a diagnostic marker for tuberculous pleural effusion. CLINICAL IMPLICATIONS: Elevated OPN level may suggest that the effusion is a seconadry to Tuberculous pleursy. However, CRP offer a better diagnostic performance than OPN.

Background The first step in the evaluation of patients with pleural effusion is to determine whe... more Background The first step in the evaluation of patients with pleural effusion is to determine whether the effusion is a transudate or an exudate. Osteopontin (OPN) is a pleiotropic integrin-binding protein with many functions. We assessed pleural effusion and serum concentrations of OPN and C-reactive protein (CRP) in patients with different types of pleural effusions. Methods The current study comprised three groups: 20 patients with transudative effusion, 30 patients with malignant effusion and 30 patients with tuberculous effusion. OPN was analysed using a commercially available enzyme-linked immunosorbent assay kit. Results OPN effusion values were significantly higher in exudates (both malignant and tuberculous effusion cases) compared with transudative effusion. Also when compared separately, patients with tuberculous effusion and those with malignant effusion had a significantly higher fluid and OPN effusion/serum ratio than those with transudative effusion. Patients with tuberculous effusion had a significantly higher serum CRP effusion and effusion/serum ratio of CRP than those with malignant or transudative effusion. Conclusion OPN is significantly increased in exudative effusion compared with transudative ones. However, serum OPN and effusion/serum OPN ratio were not significantly different in patients with malignant from those with tuberculous effusions. The lack of difference in serum OPN and effusion/serum OPN ratio between patients with malignant and those with tuberculous effusion may be attributed to the heterogeneity of the malignant effusion group. Receiver-operating characteristic (ROC) curve analysis has shown that effusion/serum CRP ratio outperformed effusion/serum OPN ratio as a diagnostic marker for tuberculous pleural effusion.

American Journal of Otolaryngology, 2004

PURPOSE: To investigate the influence of the glutathione S-transferase (GST) M1, T1, and P1 genot... more PURPOSE: To investigate the influence of the glutathione S-transferase (GST) M1, T1, and P1 genotypes on the laryngeal squamous cell carcinoma risk. MATERIAL AND METHODS: The study group consisted of 42 white patients with laryngeal squamous cell carcinoma ( ...