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Papers by nayab tahir

Polymer sciences, Mar 13, 2018

Materials Innovations

Nanoparticles range in size from 1-100 nm although much larger nanoparticles i.e. up to 300 nm, a... more Nanoparticles range in size from 1-100 nm although much larger nanoparticles i.e. up to 300 nm, are widely reported for medical application. Current trends in drug delivery research have shifted focus toward the designing of the “smart” drug delivery systems (DDS) for spacial and temporal control of the drug delivery. When a magnetic moiety is added to a DDS i.e. nanoparticle or liposome, it can be retained in a specific part of the body through localized magnetic field. These magnetically modulated drug delivery systems (MDDS) can also carry payload to deep lying tumor tissues which are difficult to target with other targeting modalities. MDDS are also used as hyperthermic agents under the influence of externally applied alternating magnetic field. Not only the magnetic hyperthermia can kill cancer cells but also causes phase-change in nanoparticles to induce abrupt drug release. Magnetic resonance imaging (MRI) is a diagnostic techniques used to image disease specific changes in t...

Advanced Technology for Delivering Therapeutics, 2017

Nanocarriers have provided the versatile platform for the delivery of various therapeutic and dia... more Nanocarriers have provided the versatile platform for the delivery of various therapeutic and diagnostic agents. Liposome, niosomes, polymeric and solid lipid nanoparticles are the most promising nanocarriers that have been entered in the clinical trials and become commercially available. However, each system has been associated with some problems that can be minimized by using the combinatorial approach of hybrid nanocarriers. These hybrid systems combine the benefits of different structural components to synergize the outcome of the therapy. In this chapter, the different types of hybrid nanocarriers have been described with particular emphasis on the brief rationale for the development of these hybrid nanocarriers along with different fabrication approaches with greater emphasize on the lipid polymer hybrid nanoparticles. A brief description factors governing the optimized response characteristics and their potential application of these hybrid nanoparticles are also presented.

Journal of the chemical society of pakistan

The present work describes a reversed phase high-performance liquid chromatography (RP-HPLC) assa... more The present work describes a reversed phase high-performance liquid chromatography (RP-HPLC) assay with diode array detection (DAD) for the determination of rhein, an active metabolite of diacerein, in spiked rabbit plasma. The plasma samples were subjected to one-step liquid-liquid extraction using a protein precipitating agent. The separation of rhein was achieved on an RP C18 hypersil-ODS column (250×4.6mm, 5μm). The mobile phase consisted of a binary mixture of methanol and water (15: 85 v/v, pH 4.7) run under the isocratic system at a flow rate of 1 ml.min-1, the wavelength of DAD was set at 210 nm. The method was validated for linearity, accuracy, precision, and stability according to the International Council for Harmonization (ICH guidelines). The calibration curve was linear over the range of 0.015-5 µg. ml-1 for rhein with the coefficient of regression (r2) of value 0.9980. Extraction recovery for rhein from rabbit plasma was in the range of 95.0% to 98.60% to different sa...

The trends of incidence, morbidity and mortality of tuberculosis in children have always remained... more The trends of incidence, morbidity and mortality of tuberculosis in children have always remained unclear because of lack of a definitive diagnostic tool in mostly cases. The diagnosis of tuberculosis in children remains a major challenge as it is seldom confirmed and is mostly based on clinical signs, symptoms and special investigations e. g. TST, chest X-ray and sputum smear microscopy. Sputum smear microscopy is positive in less than 10-15 % of children with TB and culture yields are nearly about 30-40%. In low burden countries, the triad of (a) known contact with an infectious source case, (b) a positive tuberculin skin test and (c) a suggestive CXR is usually used to establish a diagnosis of childhood TB. The research on childhood tuberculosis is often neglected due to technical difficulties like slow growth in culture, the difficulty of obtaining specimens and the diverse and relatively nonspecific clinical presentation of tuberculosis in this age group. The complexity in maki...

Pharmaceutics, 2021

The current study is aimed to fabricate doxorubicin (Dox) loaded mild temperature responsive lipo... more The current study is aimed to fabricate doxorubicin (Dox) loaded mild temperature responsive liposomes (MTLs) by thin film hydration technique for enhanced in vitro and in vivo anticancer efficacy against hepatocellular carcinoma. The aforementioned Dox loaded MTLs were developed and optimized with extrusion and drug loading techniques. The optimized MTLs were in optimum size range (118.20 ± 2.81–187.13 ± 4.15 nm), colloidal stability (−13.27 ± 0.04 to −32.34 ± 0.15 mV), and enhanced entrapment of Dox (28.71 ± 2.01–79.24 ± 2.16). Furthermore, the optimized formulation (MTL1-E(AL)) embodied improved physicochemical stability deducted by Fourier transform infra-red (FTIR) spectroscopy and mild hyperthermia-based phase transition demonstrated from differential scanning calorimetry (DSC). An in vitro drug release study revealed mild hyperthermia assisted rapid in vitro Dox release from MTLs-E(AL) (T100% ≈ 1 h) by Korsmeyer–Peppas model based Fickian diffusion (n < 0.45). Likewise, an...

Asadullah Madni*, Muhammad Imran Khan, Mubashar Rehman, Nayab Tahir, Nadia Rai, Farzana Parveen, ... more Asadullah Madni*, Muhammad Imran Khan, Mubashar Rehman, Nayab Tahir, Nadia Rai, Farzana Parveen, Muhammad Danish Saeed, Muhammad Abdur Rahim, Hassan Shah Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur, 63100, Pakistan. Riphah Institute of Pharmaceutical Sciences, Riphah International University, Lahore, Pakistan. Department of Pharmacy, University of Central Punjab, Lahore. College of Pharmacy, University of Sargodha, Sargodha. asadullah.madni@iub.edu.pk*

PLOS ONE, 2021

Pentazocine (PTZ) is a narcotic analgesic used to manage moderate to severe, acute and chronic pa... more Pentazocine (PTZ) is a narcotic analgesic used to manage moderate to severe, acute and chronic pains. In this study, PTZ loaded Ethyl cellulose microsphere has been formulated for sustained release and improved bioavailability of PTZ. These microspheres were fabricated by oil in water emulsion solvent evaporation technique. A three factorial, three levels Box-Behnken design was applied to investigate the influence of different formulation components and process variables on the formulation response using the numeric approach through the design expert® software. All the formulations were characterized for the morphology, different physicochemical properties and the results were supported with the ANOVA analysis, three dimensional contour graphs and regression equations. The maximum percentage yield was 98.67% with 98% entrapment of PTZ. The mean particle size of the formulations ranges from 50–148μm, which directly relates to the concentration of polymer and inversely proportional to...

Characterization of Pharmaceutical Nano and Microsystems, 2020

International Journal of Nanomedicine, 2020

Background: Lipid-polymer hybrid nanoparticles (LPHNP) are suitable for co-delivery of hydrophili... more Background: Lipid-polymer hybrid nanoparticles (LPHNP) are suitable for co-delivery of hydrophilic and lipophilic drugs. The structural advantages of polymers and biomimetic properties of lipids enable higher encapsulation of drugs and controlled release profile. Lipidpolymer hybrid nanoparticles have been prepared for co-delivery of curcumin and cisplatin for enhanced cytotoxicity against ovarian cancer. Material and Methods: Chitosan, cisplatin, curcumin, Lipoid S75 were selected as structural components and ionic gelation method was used for preparation of LPHNPs. Nanoparticles were formed via ionic interaction of positively charged chitosan and negatively charged lipid. Results: The optimized nanoparticles were of 225 nm with cationic charge. The encapsulation efficiency was greater than 80% with good drug loading. The drug release profile showed controlled release behavior of both curcumin and cisplatin simultaneously and the absence of burst release. The in vitro therapeutic efficacy and cellular association was evaluated using A2780 ovarian cell lines. To further investigate therapeutic efficacy, we developed 3D spheroids as tumor model to mimic the in vivo conditions. The cytotoxicity and uptake of co-loaded LPHNPs were evaluated on 3D spheroids and results indicated increased chemosensitization and enhanced therapeutic efficacy of co-loaded LPHNPs. Conclusion: Lipid-polymer hybrid nanoparticles could be a suitable platform for co-delivery of curcumin and cisplatin for enhanced cytotoxic effect on ovarian cell lines.

International Journal of Pharmaceutics, 2020

Lipid polymer hybrid nanoparticles (LPHNPs) have been merged as potential nanocarriers for treatm... more Lipid polymer hybrid nanoparticles (LPHNPs) have been merged as potential nanocarriers for treatment of cancer. In the present study, LPHNPs loaded with Sorafenib (SFN) were prepared with PLGA, Lecithin and DSPE-PEG 2000 by using the bulk nanoprecipitation and microfluidic (MF) co-flow nanoprecipitation techniques. Herein, a glass capillary microfluidic device was primed to optimize the LPHNPs and compared to the bulk nanoprecipitation method. The morphological analysis of prepared LPHNPs revealed the well-defined spherical nano-sized particles in bulk nanoprecipitation method. Whereas, core shell morphology was observed in the MF method. The formulation prepared by the MF method (MF1-MF3) indicated relatively higher % EE (95.0%, 93.8% and 88.7%) and controlled release of the SFN from the particles as compared to the LPHNPs obtained by the bulk nanoprecipitation method. However, the release of SFN from all LPHNP formulation followed Higuchi model (R 2 = 0.9901-0.9389) with Fickian diffusion mechanism. Fourier transform infrared spectroscopy (FTIR), Differential scanning calorimetry (DSC) and powder X-rays diffraction (pXRD) studies depicted the compatibility of SFN with all the structural components. In addition, the colloidal stability, in vitro cytotoxicity and cell growth inhibition studies of LPHNPs also demonstrated stability in biological media, biocompatibility and safety with enhanced anti-proliferative effects than the free SFN in breast cancer and prostate cancer cells. In conclusion, LPHNPs provided a prospective platform for the cancer chemotherapy and substantially improved the knowledge of fabrication and optimization of the hybrid nanoparticles.

Role of Novel Drug Delivery Vehicles in Nanobiomedicine [Working Title], 2019

Applications of nanotechnology and material sciences emerge in the development of various novel d... more Applications of nanotechnology and material sciences emerge in the development of various novel drug delivery systems that have been proven as promising clinically. Among these, liposomes, noisome, polymeric carriers and lipid-based delivery system were extensively explored and enter into clinical trials and clinical applications. However, each system has its own pros and cons in term of different physicochemical, pharmacokinetics and therapeutics aspects. Lipid-polymer hybrid carriers merge the potential benefit of these structural components and can be prepared by different approaches to improve the therapeutic outcomes. In this chapter, we provide the useful insight about the lipid-polymer hybrid nanoparticles (LPHNPs) that can be prepared by using the different structural components including the synthetic and natural polymers and lipids. Among these, we also explain the various methods to prepare the LPHNPs with various desired characteristics. Finally, the various therapeutic and clinical applications have been presented briefly.

International Journal of Nanomedicine, 2019

Background: Lipid polymer hybrid nanoparticles (LPHNPs) for the controlled delivery of hydrophili... more Background: Lipid polymer hybrid nanoparticles (LPHNPs) for the controlled delivery of hydrophilic doxorubicin hydrochloride (DOX.HCl) and lipophilic DOX base have been fabricated by the single step modified nanoprecipitation method. Materials and methods: Poly (D, L-lactide-co-glicolide) (PLGA), lecithin, and 1,2-distearoyl-Sn-glycero-3-phosphoethanolamine-N-[methoxy (polyethylene glycol)-2000 (DSPE-PEG 2000) were selected as structural components. Results: The mean particle size was 173-208 nm, with an encapsulation efficiency of 17.8 ±1.9 to 43.8±4.4% and 40.3±0.6 to 59. 8±1.4% for DOX.HCl and DOX base, respectively. The drug release profile was in the range 33-57% in 24 hours and followed the Higuchi model (R 2 =0.9867-0.9450) and Fickian diffusion (n<0.5). However, the release of DOX base was slower than DOX.HCl. The in vitro cytotoxicity studies and confocal imaging showed safety, good biocompatibility, and a higher degree of particle internalization. The higher internalization of DOX base was attributed to higher permeability of lipophilic component and better hydrophobic interaction of particles with cell membranes. Compared to the free DOX, the DOX.HCl and DOX base loaded LPHNPs showed higher antiproliferation effects in MDA-MB231 and PC3 cells. Conclusion: Therefore, LPHNPs have provided a potential drug delivery strategy for safe, controlled delivery of both hydrophilic and lipophilic form of DOX in cancer cells.

Acta poloniae pharmaceutica, 2016

A simple and cost effective RPLC-UV bio-analytical method was developed and used for tenoxicam qu... more A simple and cost effective RPLC-UV bio-analytical method was developed and used for tenoxicam quantification on ODS Hypersil C-18 column using classical liquid-liquid extraction technique for sample preparation. Acetonitrile was used as precipitating agent for plasma proteins and supernatant was taken for injection without any further modification. The bio-analytical method depends upon isocratic elution using binary mixture of aqueous 0.1 M potassium dihydrogen phosphate and acetonitrile in 6 : 4 ratio. The pH of mobile phase was adjusted to 2.8 which favor tenoxicam to remain undissociated throughout the analysis. The optimized flow rate of 1.0 mL/min provided proper separation of peaks and column clean up within 5 min. The UV detection was achieved at 381 nm and 4.29 min. Reproducible calibration curve gave 0.325 μg/mL LOQ, linear dynamic range from 0.325 to 20 μg/mL and recovery from plasma was 98.5% with %CV 0.2314 achieved. After validation, the method was applied in pharmaco...

Acta poloniae pharmaceutica, 2017

Tuberculosis (TB) is an infectious disease that is communicable from one person to another. Pakis... more Tuberculosis (TB) is an infectious disease that is communicable from one person to another. Pakistan stands forefront among few unfortunate countries that still have heavy burden of TB infection. Being a developing country, TB patients in Pakistan have to face different socio-economic constraints that upset life of the patients as well as their families. A cross sectional survey was conducted in three selected districts, Lodharan, Bahawalpur and Bahawalnagar during February 2011 to June 2011. From three hundred selected patients 210 Were enrolled in study after receiving written consents. Data were collected though structured questionnaire and verbal-interviews and statistically analyzes by using the univariate analysis. The survey results showed that the low educational status (p < 0.0012, CI 95%), unawareness of disease (88.7%), crowded population (p = 0.0000, CI, 95%), poverty, high treatment cost and distant access to public health facilities were directly related to prevalen...

AAPS PharmSciTech, Jan 22, 2018

Proniosomes (PN) are the dry water-soluble carrier systems that may enhance the oral bioavailabil... more Proniosomes (PN) are the dry water-soluble carrier systems that may enhance the oral bioavailability, stability, and topical permeability of therapeutic agents. The low solubility and low oral bioavailability due to extensive first pass metabolism make Pentazocine as an ideal candidate for oral and topical sustained release delivery. The present study was aimed to formulate the PNs by quick slurry method that are converted to niosomes (liquid dispersion) by hydration, and subsequently formulated to semisolid niosomal gel. The PNs were found in spherical shape in the SEM and stable in the physicochemical and thermal analysis (FTIR, TGA, and XRD). The quick slurry method produced high recovery (> 80% yield) and better flow properties (θ = 28.1-37.4°). After hydration, the niosomes exhibited desirable entrapment efficiency (44.45-76.23%), size (4.98-21.3 μm), and zeta potential (- 9.81 to - 21.53 mV). The in vitro drug release (T) was extended to more than three half-lives (2-4 h) a...

Journal of drug targeting, Jan 9, 2018

Graphene, the mother of all carbon materials has unlocked a new era of biomedical nanomaterials d... more Graphene, the mother of all carbon materials has unlocked a new era of biomedical nanomaterials due to its exceptional biocompatibility, physicochemical and mechanical properties. It is a single atom thick, nanosized, 2-dimensional structure and provides high surface area with adjustable surface chemistry to form hybrids. The present article provides a comprehensive review of ever-expanding application of graphene nanomaterials with different inorganic and organic materials in drug delivery and theranostics. Methods of preparation of nanomaterials are elaborated and biological and physicochemical characteristics of biomedical relevance are also discussed. Graphene form nanomaterials with metallic nanoparticles with multiscale application. First, graphene act as a platform to attach nanoparticles and provide excellent mechanical strength. Second, graphene improves efficacy of metallic nanoparticles in diagnostic, biosensing, therapeutic and drug delivery application. Graphene based p...

Nanoscale, Jan 19, 2017

Thermoresponsive targeting is used to deliver therapeutic agents at hyperthermic conditions (39-4... more Thermoresponsive targeting is used to deliver therapeutic agents at hyperthermic conditions (39-45 °C). However, available thermoresponsive drug delivery systems (TDDS), including liposomes, have a complex method of preparation involving toxic solvents and reagents. The objective of this in vitro study was to prepare and characterize thermoresponsive lipid nanoparticles (TLN) for treating glioblastoma, the most aggressive brain tumor whose treatment is limited by a low blood brain barrier (BBB) permeability of drugs. Thermoresponsive lipids were prepared by mixing liquid and solid fatty acids (0.1 : 1 to 2 : 1 ratio) and lipid mixtures exhibiting a solid-liquid phase transition at 39 °C were identified by plotting melting point against liquid contents. TLN were prepared by a hot melt encapsulation method using mono- or double-surfactant systems. TLN showed desirable size (<270 nm), zeta potential (-35 to -50 mV), spherical morphology and stability by FTIR studies. In the drug rel...