noor uddin - Academia.edu (original) (raw)

Papers by noor uddin

Five Schiff bases, 2-((3-chlorophenylimino)methyl)-5-(diethylamino)phenol (L1), 2-((2,4-dichlorop... more Five Schiff bases, 2-((3-chlorophenylimino)methyl)-5-(diethylamino)phenol (L1), 2-((2,4-dichlorophenylimino)
methyl)-5-(diethylamino)phenol (L2), 5-(diethylamino)-2-((3,5-dimethylphenylimino)methyl)phenol
(L3), 2-((2-chloro-4-methylphenylimino)methyl)-5-(diethylamino)phenol (L4), and 5-(diethylamino)-2-
((2,6-diethylphenylimino)methyl)phenol (L5) were synthesized and characterized by elemental analysis,
FT-IR, 1H and 13C NMR spectroscopy. Three of the compounds (L1, L2, and L4) were analyzed by single
crystal X-ray diffraction: L1 and L2 crystallized in orthorhombic P212121 and Pca21 space group,
respectively, while L4 crystallized in monoclinic P21/c space group. Theoretical investigations were performed
for all the synthesized compounds to evaluate the structural details. Drug–DNA interaction
studies results from UV–Vis spectroscopy and electrochemistry complement that the compounds bind
to DNA through electrostatic interactions. The cytotoxicity of the synthesized compounds was studied
against cancer cell lines (HeLa and MCF-7) and a normal cell line (BHK-21) by means of an MTT assay
compared to carboplatin, featuring IC50 values in the micromolar range. The pro-apoptotic mechanism
for the active compound L5 was evaluated by fluorescence microscopy, cell cycle analysis, caspase-9
and -3 activity, reactive oxygen species production, and DNA binding studies that further strengthen
the results of that L5 is a potent drug against cancer.