ajay parida - Academia.edu (original) (raw)
Papers by ajay parida
Current Science, 2018
Halophytes have superior capacity to withstand soil salinity and are appropriate resources to stu... more Halophytes have superior capacity to withstand soil salinity and are appropriate resources to study the mechanism of salt tolerance which can be harnessed to develop crops to withstand salinity. In this communication, we report the effect of salinity (200 mM NaCl) and elevated carbon dioxide (CO 2) treatments in tandem, on select halophytes that have different photosynthetic pathways: C3 and C4. The plants were raised in ambient (380 ppm) and enriched (500 ppm) concentrations of CO 2 using a mini-FACE facility. Total chlorophyll content, total soluble sugar concentration, lipid peroxidation level and electrolyte leakage were measured from fresh leaf samples collected at different time points. The results show a positive effect for elevated CO 2 concentration on salt tolerance in both C3 and C4 plants, and indicate that halophytes may benefit from rising atmospheric CO 2 concentration. The results also suggest that C4 halophytes may benefit from the rising atmospheric CO 2 concentration than C3 halophytes.
Background: The current global pandemic of Coronavirus disease 2019 (COVID-19), caused by SARS-Co... more Background: The current global pandemic of Coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2 led to the investigation with clinical, biochemical, immunological and genomic information of the patients to understand the pathophysiology of this viral infection. Methods: Samples were collected from six asymptomatic and six symptomatic SARS-CoV-2 confirmed hospitalized patients in Bhubaneswar, Odisha, India. Clinical details, biochemical parameters, treatment regime were collected from hospital, viral load was determined by RT-PCR, levels of cytokines and circulating antibodies in plasma were assessed by Bioplex and isotyping respectively. In addition, the whole genome sequencing of viral strains and mutational analysis were carried out. Findings Analysis of the biochemical parameters highlighted the increased levels of C-Reactive protein (CRP), lactate dehydrogenase (LDH), serum SGPT, serum SGOT and ferritin in symptomatic patients indicating that patients with higher levels of ...
Current Science, 2004
Twenty-six landraces of black gram collected from Orissa, India were analysed for genetic diversi... more Twenty-six landraces of black gram collected from Orissa, India were analysed for genetic diversity using amplified fragment length polymorphism (AFLP) markers. Seven primer combinations were used for AFLP analysis. The percentage polymorphism across the samples varied from 74.5 to 93%. The level of rare and common alleles contributing to the diversity in the sample was analysed using the Shannon-Weiner (SW) diversity index. The SW index revealed that three samples of the entire twenty-six contributed significantly to the overall diversity of the sample set. A dendrogram was constructed based on the UPGMA clustering method, which revealed three major clusters. A principal component analysis of the same dataset revealed similar results to that of the dendrogram, with the first principle component accounting for 58% of the total variation. The analysed samples formed five significant groups. Three samples were distinct in their clustering and remained separate from the other samples. ...
Scientific Reports
Screening the transcriptome of drought tolerant variety of little millet (Panicum sumatrense), a ... more Screening the transcriptome of drought tolerant variety of little millet (Panicum sumatrense), a marginally cultivated, nutritionally rich, susbsistent crop, can identify genes responsible for its hardiness and enable identification of new sources of genetic variation which can be used for crop improvement. RNA-Seq generated ~ 230 million reads from control and treated tissues, which were assembled into 86,614 unigenes. In silico differential gene expression analysis created an overview of patterns of gene expression during exposure to drought and salt stress. Separate gene expression profiles for leaf and root tissue revealed the differences in regulatory mechanisms operating in these tissues during exposure to abiotic stress. Several transcription factors were identified and studied for differential expression. 61 differentially expressed genes were found to be common to both tissues under drought and salinity stress and were further validated using qRT-PCR. Transcriptome of P. su...
Vaccine breakthrough infections pose a vast challenge in the eradication of the COVID pandemic si... more Vaccine breakthrough infections pose a vast challenge in the eradication of the COVID pandemic situation. Emerging SARS-CoV-2 variants of concern infecting the immunized individuals indicate an ongoing battle between host immunity and natural selection of the pathogen. Our report sheds light on the prominent SARS-CoV-2 variations observed in the isolates from AZD1222/Covishield and BBV152/Covaxin vaccinated subjects.
Covalent attachment of ubiquitin has been implicated in mediating proteolysis of the cellular pro... more Covalent attachment of ubiquitin has been implicated in mediating proteolysis of the cellular proteins by Ubiq uitin-proteasome pathway. Ubiquitin activating enzyme (EI), ubiquitin conjugating enzyme (E2), and ubiquitin pro tein ligase (E3) are the three enzymes involved in this process. This paper reports the isolation of a gene that codes for the ubiquitin conjugating enzyme in Avicennia marilla (AmUBC2), and regulation of its expression at RNA level under salt stress. Deduced amino acid sequence of AmUBC2 showed 96% identity with UBC2 of Arabidopsis thalialla and also 73-78 % identity with RAD6 DNA repair protein of Homo sapiens, Rattus llorvegicus, Caenorhabditis elegalls, Drosophila melallogaster, Arabidopsis thalialla and Saccharomyces cerevisiae. Multiple alignment analysis showed that the amino acid residues in the core region of UBC2 were highly conserved across different taxa in the evolution ar y hierarchy. While some ubiquitin conjugating enzymes were induced under sa...
Communications Biology
Water scarcity and salinity are major challenges facing agriculture today, which can be addressed... more Water scarcity and salinity are major challenges facing agriculture today, which can be addressed by engineering plants to grow in the boundless seawater. Understanding the mangrove plants at the molecular level will be necessary for developing such highly salt-tolerant agricultural crops. With this objective, we sequenced the genome of a salt-secreting and extraordinarily salt-tolerant mangrove species, Avicennia marina, that grows optimally in 75% seawater and tolerates >250% seawater. Our reference-grade ~457 Mb genome contains 31 scaffolds corresponding to its chromosomes. We identified 31,477 protein-coding genes and a salinome consisting of 3246 salinity-responsive genes and homologs of 614 experimentally validated salinity tolerance genes. The salinome provides a strong foundation to understand the molecular mechanisms of salinity tolerance in plants and breeding crops suitable for seawater farming.
The FASEB Journal
Syrian golden hamsters (Mesocricetus auratus) infected by severe acute respiratory syndrome coron... more Syrian golden hamsters (Mesocricetus auratus) infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) manifests lung pathology. In this study, efforts were made to check the infectivity of a local SARS-CoV-2 isolate in a selflimiting and non-lethal hamster model and evaluate the differential expression of lung proteins during acute infection and convalescence. The findings of this study confirm the infectivity of this isolate in vivo. Analysis of clinical parameters and tissue samples show the pathophysiological manifestation of SARS-CoV-2 infection similar to that reported earlier in COVID-19 patients and hamsters infected with other isolates. However, diffuse alveolar damage (DAD), a common histopathological feature of human COVID-19 was only occasionally noticed. The lung-associated pathological changes were very prominent on the 4th day post-infection (dpi), mostly resolved by 14 dpi. Here, we carried out the quantitative proteomic analysis of the lung tissues from SARS-CoV-2-infected hamsters on day 4 and day 14 postinfection. This resulted in the identification of 1585 proteins of which 68 proteins were significantly altered between both the infected groups. Pathway analysis revealed complement and coagulation cascade, platelet activation, ferroptosis, and focal adhesion as the top enriched pathways. In addition, we also identified altered expression of two pulmonary surfactant-associated proteins (Sftpd and Sftpb), known for their protective role in lung function. Together, these findings will aid in understanding the mechanism(s) involved in SARS-CoV-2 pathogenesis and progression of the disease.
International Journal of Infectious Diseases
Frontiers in Microbiology
Coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus ... more Coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, has emerged as a global pandemic worldwide. In this study, we used ARTIC primers–based amplicon sequencing to profile 225 SARS-CoV-2 genomes from India. Phylogenetic analysis of 202 high-quality assemblies identified the presence of all the five reported clades 19A, 19B, 20A, 20B, and 20C in the population. The analyses revealed Europe and Southeast Asia as two major routes for introduction of the disease in India followed by local transmission. Interestingly, the19B clade was found to be more prevalent in our sequenced genomes (17%) compared to other genomes reported so far from India. Haplotype network analysis showed evolution of 19A and 19B clades in parallel from predominantly Gujarat state in India, suggesting it to be one of the major routes of disease transmission in India during the months of March and April, whereas 20B and 20C appeared to evolve from 20A....
In infectious diseases, the routes of transmission play major roles in determining the rate and e... more In infectious diseases, the routes of transmission play major roles in determining the rate and extent of disease spread. Though fomites and aerosol droplets are major sources of SARS-CoV-2 human to human transmission, studies have also reported possible involvement of other routes of transmission like fecal-oral. Multiple studies around the world have reported shedding of the SARS-CoV-2 viral genome in certain COVID-19 patient fecal samples. Hence, the major objective of this study was to get the experimental evidence whether in Indian COVID-19 patients fecal dissemination of the SARS-CoV-2 genome occurs or not. Information obtained from twelve number of patients from a COVID-19 hospital of Odisha has demonstrated that both symptomatic and asymptomatic Indian patients could be positive for the SARS-CoV-2 genome in their fecal component. The findings have also established a protocol to collect and extract viral RNA for SARS-CoV-2 detection in fecal samples. Together, the study suppo...
Current Plant Biology
Abstract Abiotic stresses affect crop productivity worldwide. Understanding molecular mechanisms ... more Abstract Abiotic stresses affect crop productivity worldwide. Understanding molecular mechanisms of plant abiotic stress tolerance is important for developing stress tolerant crop plants for sustaining crop productivity in future. Chrysopogon zizanioides (vetiver) is a perennial C4 grass reported to be tolerant to water, salinity and submergence stress. Here, we subjected C. zizanioides seedlings to salt and drought stress and carried out whole transcriptome profiling of leaf and root tissues. Assessing the global transcriptome changes under drought and salt stress resulted in the identification of several genes contributing to stress response in this species. Overall, more transcriptomic changes were observed in leaf tissue compared to root tissue. The response to either stress manifested primarily as upregulation of gene expression in both leaf and root. The study identified stress responsive genes commonly and differently regulated under stress/tissue conditions. Several DEGs in our data were identified as enzymes involved in biosynthesis of essential oil components. The differential expression of these genes under drought and salt stress may affect the vetiver essential oil composition under these stresses. Similarly, several genes involved in the biosynthesis of benzylisoquinoline alkaloids including morphin were found to be differentially expressing in our data. Our data may facilitate further molecular studies on stress tolerance of C. zizanioides. The DEGs from our results are potential candidates for understanding and engineering abiotic stress tolerance in plants.
![Research paper thumbnail of T-homoeolog specific plasma membrane protein 3 [ Nt(t)PMP3-2 ] in polyploid Nicotiana tabacum shows conserved alternative splicing, derived from extant Nicotiana tomentosiformis parent](https://attachments.academia-assets.com/81127759/thumbnails/1.jpg)
](Plant Physiology and Biochemistry
COVID-19 that emerged as a global pandemic is caused by SARS-CoV-2 virus. The virus genome analys... more COVID-19 that emerged as a global pandemic is caused by SARS-CoV-2 virus. The virus genome analysis during disease spread reveals about its evolution and transmission. We did whole genome sequencing of 225 clinical strains from the state of Odisha in eastern India using ARTIC protocol-based amplicon sequencing. Phylogenetic analysis identified the presence of all five reported clades 19A, 19B, 20A, 20B and 20C in the population. The analyses revealed two major routes for the introduction of the disease in India i.e. Europe and South-east Asia followed by local transmission. Interestingly, 19B clade was found to be much more prevalent in our sequenced genomes (17%) as compared to other genomes reported so far from India. The haplogroup analysis for clades showed evolution of 19A and 19B in parallel whereas the 20B and 20C appeared to evolve from 20A. Majority of the 19A and 19B clades were present in cases that migrated from Gujarat state in India suggesting it to be one of the major...
Molecular Biology Reports, Mar 1, 2011
Plant growth and productivity are adversely affected by various abiotic and biotic stress factors... more Plant growth and productivity are adversely affected by various abiotic and biotic stress factors. In our previous study, we used Prosopis juliflora, an abiotic stress tolerant tree species of Fabaceae, as a model plant system for isolating genes functioning in abiotic stress tolerance. Here we report the isolation and characterization of a Rab family GTPase from P. juliflora (Pj Rab7) and the ability of this gene to confer salt stress tolerance in transgenic tobacco. Northern analysis for Pj Rab7 in P. juliflora leaf tissue revealed up-regulation of this gene under salt stress under the concentrations and time points analyzed. Pj Rab7 transgenic tobacco lines survived better under conditions of 150 mM NaCl stress compared to control un-transformed plants. Pj Rab7 transgenic plants were found to accumulate more sodium than control plants during salt stress. The results of our studies could be used as a starting point for generation of crop plants tolerant to abiotic stress.
Canadian Journal of Biotechnology
Current Science, 2018
Halophytes have superior capacity to withstand soil salinity and are appropriate resources to stu... more Halophytes have superior capacity to withstand soil salinity and are appropriate resources to study the mechanism of salt tolerance which can be harnessed to develop crops to withstand salinity. In this communication, we report the effect of salinity (200 mM NaCl) and elevated carbon dioxide (CO 2) treatments in tandem, on select halophytes that have different photosynthetic pathways: C3 and C4. The plants were raised in ambient (380 ppm) and enriched (500 ppm) concentrations of CO 2 using a mini-FACE facility. Total chlorophyll content, total soluble sugar concentration, lipid peroxidation level and electrolyte leakage were measured from fresh leaf samples collected at different time points. The results show a positive effect for elevated CO 2 concentration on salt tolerance in both C3 and C4 plants, and indicate that halophytes may benefit from rising atmospheric CO 2 concentration. The results also suggest that C4 halophytes may benefit from the rising atmospheric CO 2 concentration than C3 halophytes.
Background: The current global pandemic of Coronavirus disease 2019 (COVID-19), caused by SARS-Co... more Background: The current global pandemic of Coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2 led to the investigation with clinical, biochemical, immunological and genomic information of the patients to understand the pathophysiology of this viral infection. Methods: Samples were collected from six asymptomatic and six symptomatic SARS-CoV-2 confirmed hospitalized patients in Bhubaneswar, Odisha, India. Clinical details, biochemical parameters, treatment regime were collected from hospital, viral load was determined by RT-PCR, levels of cytokines and circulating antibodies in plasma were assessed by Bioplex and isotyping respectively. In addition, the whole genome sequencing of viral strains and mutational analysis were carried out. Findings Analysis of the biochemical parameters highlighted the increased levels of C-Reactive protein (CRP), lactate dehydrogenase (LDH), serum SGPT, serum SGOT and ferritin in symptomatic patients indicating that patients with higher levels of ...
Current Science, 2004
Twenty-six landraces of black gram collected from Orissa, India were analysed for genetic diversi... more Twenty-six landraces of black gram collected from Orissa, India were analysed for genetic diversity using amplified fragment length polymorphism (AFLP) markers. Seven primer combinations were used for AFLP analysis. The percentage polymorphism across the samples varied from 74.5 to 93%. The level of rare and common alleles contributing to the diversity in the sample was analysed using the Shannon-Weiner (SW) diversity index. The SW index revealed that three samples of the entire twenty-six contributed significantly to the overall diversity of the sample set. A dendrogram was constructed based on the UPGMA clustering method, which revealed three major clusters. A principal component analysis of the same dataset revealed similar results to that of the dendrogram, with the first principle component accounting for 58% of the total variation. The analysed samples formed five significant groups. Three samples were distinct in their clustering and remained separate from the other samples. ...
Scientific Reports
Screening the transcriptome of drought tolerant variety of little millet (Panicum sumatrense), a ... more Screening the transcriptome of drought tolerant variety of little millet (Panicum sumatrense), a marginally cultivated, nutritionally rich, susbsistent crop, can identify genes responsible for its hardiness and enable identification of new sources of genetic variation which can be used for crop improvement. RNA-Seq generated ~ 230 million reads from control and treated tissues, which were assembled into 86,614 unigenes. In silico differential gene expression analysis created an overview of patterns of gene expression during exposure to drought and salt stress. Separate gene expression profiles for leaf and root tissue revealed the differences in regulatory mechanisms operating in these tissues during exposure to abiotic stress. Several transcription factors were identified and studied for differential expression. 61 differentially expressed genes were found to be common to both tissues under drought and salinity stress and were further validated using qRT-PCR. Transcriptome of P. su...
Vaccine breakthrough infections pose a vast challenge in the eradication of the COVID pandemic si... more Vaccine breakthrough infections pose a vast challenge in the eradication of the COVID pandemic situation. Emerging SARS-CoV-2 variants of concern infecting the immunized individuals indicate an ongoing battle between host immunity and natural selection of the pathogen. Our report sheds light on the prominent SARS-CoV-2 variations observed in the isolates from AZD1222/Covishield and BBV152/Covaxin vaccinated subjects.
Covalent attachment of ubiquitin has been implicated in mediating proteolysis of the cellular pro... more Covalent attachment of ubiquitin has been implicated in mediating proteolysis of the cellular proteins by Ubiq uitin-proteasome pathway. Ubiquitin activating enzyme (EI), ubiquitin conjugating enzyme (E2), and ubiquitin pro tein ligase (E3) are the three enzymes involved in this process. This paper reports the isolation of a gene that codes for the ubiquitin conjugating enzyme in Avicennia marilla (AmUBC2), and regulation of its expression at RNA level under salt stress. Deduced amino acid sequence of AmUBC2 showed 96% identity with UBC2 of Arabidopsis thalialla and also 73-78 % identity with RAD6 DNA repair protein of Homo sapiens, Rattus llorvegicus, Caenorhabditis elegalls, Drosophila melallogaster, Arabidopsis thalialla and Saccharomyces cerevisiae. Multiple alignment analysis showed that the amino acid residues in the core region of UBC2 were highly conserved across different taxa in the evolution ar y hierarchy. While some ubiquitin conjugating enzymes were induced under sa...
Communications Biology
Water scarcity and salinity are major challenges facing agriculture today, which can be addressed... more Water scarcity and salinity are major challenges facing agriculture today, which can be addressed by engineering plants to grow in the boundless seawater. Understanding the mangrove plants at the molecular level will be necessary for developing such highly salt-tolerant agricultural crops. With this objective, we sequenced the genome of a salt-secreting and extraordinarily salt-tolerant mangrove species, Avicennia marina, that grows optimally in 75% seawater and tolerates >250% seawater. Our reference-grade ~457 Mb genome contains 31 scaffolds corresponding to its chromosomes. We identified 31,477 protein-coding genes and a salinome consisting of 3246 salinity-responsive genes and homologs of 614 experimentally validated salinity tolerance genes. The salinome provides a strong foundation to understand the molecular mechanisms of salinity tolerance in plants and breeding crops suitable for seawater farming.
The FASEB Journal
Syrian golden hamsters (Mesocricetus auratus) infected by severe acute respiratory syndrome coron... more Syrian golden hamsters (Mesocricetus auratus) infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) manifests lung pathology. In this study, efforts were made to check the infectivity of a local SARS-CoV-2 isolate in a selflimiting and non-lethal hamster model and evaluate the differential expression of lung proteins during acute infection and convalescence. The findings of this study confirm the infectivity of this isolate in vivo. Analysis of clinical parameters and tissue samples show the pathophysiological manifestation of SARS-CoV-2 infection similar to that reported earlier in COVID-19 patients and hamsters infected with other isolates. However, diffuse alveolar damage (DAD), a common histopathological feature of human COVID-19 was only occasionally noticed. The lung-associated pathological changes were very prominent on the 4th day post-infection (dpi), mostly resolved by 14 dpi. Here, we carried out the quantitative proteomic analysis of the lung tissues from SARS-CoV-2-infected hamsters on day 4 and day 14 postinfection. This resulted in the identification of 1585 proteins of which 68 proteins were significantly altered between both the infected groups. Pathway analysis revealed complement and coagulation cascade, platelet activation, ferroptosis, and focal adhesion as the top enriched pathways. In addition, we also identified altered expression of two pulmonary surfactant-associated proteins (Sftpd and Sftpb), known for their protective role in lung function. Together, these findings will aid in understanding the mechanism(s) involved in SARS-CoV-2 pathogenesis and progression of the disease.
International Journal of Infectious Diseases
Frontiers in Microbiology
Coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus ... more Coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, has emerged as a global pandemic worldwide. In this study, we used ARTIC primers–based amplicon sequencing to profile 225 SARS-CoV-2 genomes from India. Phylogenetic analysis of 202 high-quality assemblies identified the presence of all the five reported clades 19A, 19B, 20A, 20B, and 20C in the population. The analyses revealed Europe and Southeast Asia as two major routes for introduction of the disease in India followed by local transmission. Interestingly, the19B clade was found to be more prevalent in our sequenced genomes (17%) compared to other genomes reported so far from India. Haplotype network analysis showed evolution of 19A and 19B clades in parallel from predominantly Gujarat state in India, suggesting it to be one of the major routes of disease transmission in India during the months of March and April, whereas 20B and 20C appeared to evolve from 20A....
In infectious diseases, the routes of transmission play major roles in determining the rate and e... more In infectious diseases, the routes of transmission play major roles in determining the rate and extent of disease spread. Though fomites and aerosol droplets are major sources of SARS-CoV-2 human to human transmission, studies have also reported possible involvement of other routes of transmission like fecal-oral. Multiple studies around the world have reported shedding of the SARS-CoV-2 viral genome in certain COVID-19 patient fecal samples. Hence, the major objective of this study was to get the experimental evidence whether in Indian COVID-19 patients fecal dissemination of the SARS-CoV-2 genome occurs or not. Information obtained from twelve number of patients from a COVID-19 hospital of Odisha has demonstrated that both symptomatic and asymptomatic Indian patients could be positive for the SARS-CoV-2 genome in their fecal component. The findings have also established a protocol to collect and extract viral RNA for SARS-CoV-2 detection in fecal samples. Together, the study suppo...
Current Plant Biology
Abstract Abiotic stresses affect crop productivity worldwide. Understanding molecular mechanisms ... more Abstract Abiotic stresses affect crop productivity worldwide. Understanding molecular mechanisms of plant abiotic stress tolerance is important for developing stress tolerant crop plants for sustaining crop productivity in future. Chrysopogon zizanioides (vetiver) is a perennial C4 grass reported to be tolerant to water, salinity and submergence stress. Here, we subjected C. zizanioides seedlings to salt and drought stress and carried out whole transcriptome profiling of leaf and root tissues. Assessing the global transcriptome changes under drought and salt stress resulted in the identification of several genes contributing to stress response in this species. Overall, more transcriptomic changes were observed in leaf tissue compared to root tissue. The response to either stress manifested primarily as upregulation of gene expression in both leaf and root. The study identified stress responsive genes commonly and differently regulated under stress/tissue conditions. Several DEGs in our data were identified as enzymes involved in biosynthesis of essential oil components. The differential expression of these genes under drought and salt stress may affect the vetiver essential oil composition under these stresses. Similarly, several genes involved in the biosynthesis of benzylisoquinoline alkaloids including morphin were found to be differentially expressing in our data. Our data may facilitate further molecular studies on stress tolerance of C. zizanioides. The DEGs from our results are potential candidates for understanding and engineering abiotic stress tolerance in plants.
Plant Physiology and Biochemistry
COVID-19 that emerged as a global pandemic is caused by SARS-CoV-2 virus. The virus genome analys... more COVID-19 that emerged as a global pandemic is caused by SARS-CoV-2 virus. The virus genome analysis during disease spread reveals about its evolution and transmission. We did whole genome sequencing of 225 clinical strains from the state of Odisha in eastern India using ARTIC protocol-based amplicon sequencing. Phylogenetic analysis identified the presence of all five reported clades 19A, 19B, 20A, 20B and 20C in the population. The analyses revealed two major routes for the introduction of the disease in India i.e. Europe and South-east Asia followed by local transmission. Interestingly, 19B clade was found to be much more prevalent in our sequenced genomes (17%) as compared to other genomes reported so far from India. The haplogroup analysis for clades showed evolution of 19A and 19B in parallel whereas the 20B and 20C appeared to evolve from 20A. Majority of the 19A and 19B clades were present in cases that migrated from Gujarat state in India suggesting it to be one of the major...
Molecular Biology Reports, Mar 1, 2011
Plant growth and productivity are adversely affected by various abiotic and biotic stress factors... more Plant growth and productivity are adversely affected by various abiotic and biotic stress factors. In our previous study, we used Prosopis juliflora, an abiotic stress tolerant tree species of Fabaceae, as a model plant system for isolating genes functioning in abiotic stress tolerance. Here we report the isolation and characterization of a Rab family GTPase from P. juliflora (Pj Rab7) and the ability of this gene to confer salt stress tolerance in transgenic tobacco. Northern analysis for Pj Rab7 in P. juliflora leaf tissue revealed up-regulation of this gene under salt stress under the concentrations and time points analyzed. Pj Rab7 transgenic tobacco lines survived better under conditions of 150 mM NaCl stress compared to control un-transformed plants. Pj Rab7 transgenic plants were found to accumulate more sodium than control plants during salt stress. The results of our studies could be used as a starting point for generation of crop plants tolerant to abiotic stress.
Canadian Journal of Biotechnology