patrick boyaval - Academia.edu (original) (raw)
Papers by patrick boyaval
HAL (Le Centre pour la Communication Scientifique Directe), Sep 26, 2000
Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen), 2001
In vivo 13C Nuclear Magnetic Resonance (NMR) spectroscopy was used to investigate the pathways of... more In vivo 13C Nuclear Magnetic Resonance (NMR) spectroscopy was used to investigate the pathways of glucose metabolism, non-invasively, in living cell suspensions of Propionibacterium freudenreichii subsp. shermanii. This species is the main ripening flora of the Swiss-type cheeses and is widely used as propionic acid and vitamin B12 industrial producer. The flow of labelled [1-13C]glucose was monitored in living cell suspensions and enrichment was detected in main products like [1-13C]glycogen, [6-13C]lycogen, [1-13C]trehalose, [6-13C]trehalose, [1-13C]propionate, [2-13C]propionate, [3-13C]propionate, [1-13C]acetate, [2-13C]acetate, [1-13C]succinate, [2-13C]succinate and [1-13C]CO2. alpha and beta glucose consumption could be examined separately and were catabolized at the same rate. Three intermediates were also found out, namely [1-13C]glucose-6-phosphate, [6-13C]glucose-6-phosphate and [1-13C]glucose-1-phosphate. From the formation of intermediates such as [6-3C]glucose-6-phosphat...
HAL (Le Centre pour la Communication Scientifique Directe), 1989
Enzyme and Microbial Technology, 1988
Whole cells of Lactobacillus helveticus were immobilized in calcium-alginate beads to produce lac... more Whole cells of Lactobacillus helveticus were immobilized in calcium-alginate beads to produce lactic acid from cheese whey ultrafiltrate. Ca-alginate-entrapped cells were characterized by higher fermentation rates and optimum pH than free cells. No difference could be observed in the profile of cell activity against temperature for either type of cells. After a heat treatment, cell activity was higher for free cells than for immobilized cells. Continuous lactic acid fermentation using a packed bed reactor was investigated.
The fast milk acidifying phenotype of Streptococcus thermophilus can be acquired by natural trans... more The fast milk acidifying phenotype of Streptococcus thermophilus can be acquired by natural transformation of the genomic island encoding the cell-envelope proteinase PrtS
Dairy propionibacteria, and especially Propionibacterium freudenreichii subsp. shermanii, are imp... more Dairy propionibacteria, and especially Propionibacterium freudenreichii subsp. shermanii, are important in the food industry and biotechnology. Only a few investigations have focused on the complex physiology of this remarkable bacterium, while the physiology of dairy lactic acid bacteria has been extensively studied over the past decades. Here we report the progress of our whole genome sequencing project: 93% of the P. shermanii genome was assembled in 426 contigs with low overall redundancy. Our annotation strategy overlaps the sequence finishing step, thus improving it. Annotation of the incomplete genome is performed using ContigBrowser, a bioinformatic tool allowing data management, developed in our laboratory. This resulted in the detection of 2611 putative proteins (data May 2003). Our tool allows an expert annotation by manual verification and curation of functional protein categories after automatic assignment. Our genomic sequence analysis, combined with the already develo...
Le Centre pour la Communication Scientifique Directe - HAL - Diderot, 2001
46 ref.International audienc
Journal of Fungi
Food processes use different microorganisms, from bacteria to fungi. Yeast strains have been exte... more Food processes use different microorganisms, from bacteria to fungi. Yeast strains have been extensively studied, especially Saccharomyces cerevisiae. However, to date, very little is known about the potential beneficial effects of molds on gut health as part of gut microbiota. We undertook a comprehensive characterization of five mold strains, Penicillium camemberti, P. nalgiovense, P. roqueforti, Fusarium domesticum, and Geotrichum candidum used in food processes, on their ability to trigger or protect intestinal inflammation using in vitro human cell models and in vivo susceptibility to sodium dextran sulfate-induced colitis. Comparison of spore adhesion to epithelial cells showed a very wide disparity in results, with F. domesticum and P. roqueforti being the two extremes, with almost no adhesion and 20% adhesion, respectively. Interaction with human immune cells showed mild pro-inflammatory properties of all Penicillium strains and no effect of the others. However, the potentia...
Depuis déjà deux ans, la Région Bretagne s'est résolument tournée vers les biotechnologies. Le ch... more Depuis déjà deux ans, la Région Bretagne s'est résolument tournée vers les biotechnologies. Le challenge est d'envergure mais les prémices laissent bien augurer du résultat. La mobilisation est 1 importante : laboratoires universitaires, institutionnels et privés, directeurs généraux d'entreprises et centres de transferts, chacun remplissant son rôle vers un même o'bj-ectif pour permettre à l'économie régionale de pénétrer sur le marché des produits obtenus par l'application de procédés biotechnologiques. Les fermentations tiennent une place privilégiée dès que l'on parle de bio-industries et sur ce , i;egistre, la Br, etagne était b'Îen peu pourvue. 11 fallait donc étudier, rassurer, poser les bonnes questions, se référer à l'existant, explorer le devenir. C'est dans ce sens que Mess, ieurs BOYAVAL, BROUSSOLLE et BRULE ont réalisé le travail présenté dans l'artiic• le suivant ; travail qui permettra aux entreprises de mieux préparer une démarche d'1 intégration de procédés «bio» dans leurs chaînes de fabrication, aux centres de transfert de convaincre sur des exemples concrets et aux chercheurs d'espérer voir une application de leurs travaux d'acquisition de connaissances fondamentales et de développement de technologies en découlant. Mais ce trava, il ne doit être qu'un début ; il faut maintenant aller plus loin et réafüer. Pour cela, le Programme BR.ITT A apporte chaque jour des éléments dynamiques de réussite. La Région Bretagne sera, dans les dix années à venir, une référence dans l'util 1 isation des bic-procédés. Les produits fabriqués ayant gagné en plus-value quitteront .Jes entrepriises bretonnes pour conquérir le consommateur par leur niveau , de qualité contrôMe et gar~_ ntie. C'est le décollage que, tous ensemble, nous devons réussir.
– The solubility and the selectivity of αS1-, β- and κ-casein isolation from an industri-ally pro... more – The solubility and the selectivity of αS1-, β- and κ-casein isolation from an industri-ally produced spray-dried sodium caseinate as a function of temperature (20–40 ◦C) and of ethanol volume fraction (0–0.6) at pH 5.0–7.5 were studied. Reverse-phase high-performance liquid chro-matography and nitrogen balance were used as analytical methods. At 20 ◦C, casein solubility as a function of pH (5–7) and of the ethanol volume fraction (0–0.6) is quite well represented by an equation describing the ionisation process. αS1-, β- and κ-casein solubility and β- and κ-casein ex-traction selectivity decrease both with a rise in the ethanol volume fraction to 0.45–0.50 and with temperature increase. For αS1-casein, the selectivity increases to 67 % for an ethanol volume fraction of 0.47 at 29 ◦C. This fraction could be used as an emulsifying agent or for cream liqueur production or further purification.
Le domaine de l'invention est celui de la fermentation semi-continue d'une source carbone... more Le domaine de l'invention est celui de la fermentation semi-continue d'une source carbonee pour la fabrication d'un melange biologique a base d'acide propionique, selon un processus industrialisable. Le dispositif selon l'invention comprend avantageusement une cuve de fermentation (6) comportant deux boucles externes (9, 19) de circulation du substrat fermente: une boucle (9) de brassage du substrat fermente effectuant un prelevement et une reintroduction du substrat fermente dans la cuve (6) de fermentation, une boucle (19) d'extraction du melange (21) biologique a base d'acide propionique fabrique (21), incluant un module (22) de separation melange biologique fabrique (21)/bacteries (23). Le procede mis en ÷uvre est du type semi-continu, forme d'une succession de cycles de fermentation/extraction avec recyclage integral de la biomasse, puis recyclage partiel apres obtention d'une concentration en biomasse predeterminee.
FEMS Microbiology Reviews, 2020
Maternal health status is vital for the development of the offspring of humans, including physiol... more Maternal health status is vital for the development of the offspring of humans, including physiological health and psychological functions. The complex and diverse microbial ecosystem residing within humans contributes critically to these intergenerational impacts. Perinatal factors, including maternal nutrition, antibiotic use and maternal stress, alter the maternal gut microbiota during pregnancy, which can be transmitted to the offspring. In addition, gestational age at birth and mode of delivery are indicated frequently to modulate the acquisition and development of gut microbiota in early life. The early-life gut microbiota engages in a range of host biological processes, particularly immunity, cognitive neurodevelopment and metabolism. The perturbed early-life gut microbiota increases the risk for disease in early and later life, highlighting the importance of understanding relationships of perinatal factors with early-life microbial composition and functions. In this review, ...
Le Lait, 1993
HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific re... more HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.
Département de biochimie, de microbiologie et de bio-informatique, Faculté des sciences et de gén... more Département de biochimie, de microbiologie et de bio-informatique, Faculté des sciences et de génie, Groupe de recherche en écologie buccale, Faculté de médecine dentaire, Félix d’Hérelle Reference Center for Bacterial Viruses, Université Laval, Quebec City, Quebec, Canada, G1V 0A6. 2 Danisco USA Inc., 3329 Agriculture Drive, Madison, WI 53716, USA. 3 Danisco France SAS, Boîte Postale 10, F-86220 Dangé-Saint-Romain, France.
La presente invention concerne des procedes et des compositions permettant de moduler la resistan... more La presente invention concerne des procedes et des compositions permettant de moduler la resistance d'une cellule a un acide nucleique cible ou a un produit de la transcription de ce dernier. Dans des modes de realisation preferes, l'invention concerne des compositions et des procedes permettant d'utiliser un ou plusieurs genes ou proteines cas pour moduler la resistance d'une cellule a un acide nucleique cible ou a un produit de transcription de ce dernier. Dans des modes de realisation, la presente invention concerne des procedes et des compositions qui peuvent servir a mettre au point et a utiliser des combinaisons de souches et des rotations de cultures starter. Dans d'autres modes de realisation, la presente invention concerne des procedes de marquage et/ou d'identification de bacteries. Dans des modes de realisation preferes, la presente invention concerne des procedes consistant a utiliser des loci CRISPR pour determiner la virulence potentielle d'...
HAL (Le Centre pour la Communication Scientifique Directe), Sep 26, 2000
Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen), 2001
In vivo 13C Nuclear Magnetic Resonance (NMR) spectroscopy was used to investigate the pathways of... more In vivo 13C Nuclear Magnetic Resonance (NMR) spectroscopy was used to investigate the pathways of glucose metabolism, non-invasively, in living cell suspensions of Propionibacterium freudenreichii subsp. shermanii. This species is the main ripening flora of the Swiss-type cheeses and is widely used as propionic acid and vitamin B12 industrial producer. The flow of labelled [1-13C]glucose was monitored in living cell suspensions and enrichment was detected in main products like [1-13C]glycogen, [6-13C]lycogen, [1-13C]trehalose, [6-13C]trehalose, [1-13C]propionate, [2-13C]propionate, [3-13C]propionate, [1-13C]acetate, [2-13C]acetate, [1-13C]succinate, [2-13C]succinate and [1-13C]CO2. alpha and beta glucose consumption could be examined separately and were catabolized at the same rate. Three intermediates were also found out, namely [1-13C]glucose-6-phosphate, [6-13C]glucose-6-phosphate and [1-13C]glucose-1-phosphate. From the formation of intermediates such as [6-3C]glucose-6-phosphat...
HAL (Le Centre pour la Communication Scientifique Directe), 1989
Enzyme and Microbial Technology, 1988
Whole cells of Lactobacillus helveticus were immobilized in calcium-alginate beads to produce lac... more Whole cells of Lactobacillus helveticus were immobilized in calcium-alginate beads to produce lactic acid from cheese whey ultrafiltrate. Ca-alginate-entrapped cells were characterized by higher fermentation rates and optimum pH than free cells. No difference could be observed in the profile of cell activity against temperature for either type of cells. After a heat treatment, cell activity was higher for free cells than for immobilized cells. Continuous lactic acid fermentation using a packed bed reactor was investigated.
The fast milk acidifying phenotype of Streptococcus thermophilus can be acquired by natural trans... more The fast milk acidifying phenotype of Streptococcus thermophilus can be acquired by natural transformation of the genomic island encoding the cell-envelope proteinase PrtS
Dairy propionibacteria, and especially Propionibacterium freudenreichii subsp. shermanii, are imp... more Dairy propionibacteria, and especially Propionibacterium freudenreichii subsp. shermanii, are important in the food industry and biotechnology. Only a few investigations have focused on the complex physiology of this remarkable bacterium, while the physiology of dairy lactic acid bacteria has been extensively studied over the past decades. Here we report the progress of our whole genome sequencing project: 93% of the P. shermanii genome was assembled in 426 contigs with low overall redundancy. Our annotation strategy overlaps the sequence finishing step, thus improving it. Annotation of the incomplete genome is performed using ContigBrowser, a bioinformatic tool allowing data management, developed in our laboratory. This resulted in the detection of 2611 putative proteins (data May 2003). Our tool allows an expert annotation by manual verification and curation of functional protein categories after automatic assignment. Our genomic sequence analysis, combined with the already develo...
Le Centre pour la Communication Scientifique Directe - HAL - Diderot, 2001
46 ref.International audienc
Journal of Fungi
Food processes use different microorganisms, from bacteria to fungi. Yeast strains have been exte... more Food processes use different microorganisms, from bacteria to fungi. Yeast strains have been extensively studied, especially Saccharomyces cerevisiae. However, to date, very little is known about the potential beneficial effects of molds on gut health as part of gut microbiota. We undertook a comprehensive characterization of five mold strains, Penicillium camemberti, P. nalgiovense, P. roqueforti, Fusarium domesticum, and Geotrichum candidum used in food processes, on their ability to trigger or protect intestinal inflammation using in vitro human cell models and in vivo susceptibility to sodium dextran sulfate-induced colitis. Comparison of spore adhesion to epithelial cells showed a very wide disparity in results, with F. domesticum and P. roqueforti being the two extremes, with almost no adhesion and 20% adhesion, respectively. Interaction with human immune cells showed mild pro-inflammatory properties of all Penicillium strains and no effect of the others. However, the potentia...
Depuis déjà deux ans, la Région Bretagne s'est résolument tournée vers les biotechnologies. Le ch... more Depuis déjà deux ans, la Région Bretagne s'est résolument tournée vers les biotechnologies. Le challenge est d'envergure mais les prémices laissent bien augurer du résultat. La mobilisation est 1 importante : laboratoires universitaires, institutionnels et privés, directeurs généraux d'entreprises et centres de transferts, chacun remplissant son rôle vers un même o'bj-ectif pour permettre à l'économie régionale de pénétrer sur le marché des produits obtenus par l'application de procédés biotechnologiques. Les fermentations tiennent une place privilégiée dès que l'on parle de bio-industries et sur ce , i;egistre, la Br, etagne était b'Îen peu pourvue. 11 fallait donc étudier, rassurer, poser les bonnes questions, se référer à l'existant, explorer le devenir. C'est dans ce sens que Mess, ieurs BOYAVAL, BROUSSOLLE et BRULE ont réalisé le travail présenté dans l'artiic• le suivant ; travail qui permettra aux entreprises de mieux préparer une démarche d'1 intégration de procédés «bio» dans leurs chaînes de fabrication, aux centres de transfert de convaincre sur des exemples concrets et aux chercheurs d'espérer voir une application de leurs travaux d'acquisition de connaissances fondamentales et de développement de technologies en découlant. Mais ce trava, il ne doit être qu'un début ; il faut maintenant aller plus loin et réafüer. Pour cela, le Programme BR.ITT A apporte chaque jour des éléments dynamiques de réussite. La Région Bretagne sera, dans les dix années à venir, une référence dans l'util 1 isation des bic-procédés. Les produits fabriqués ayant gagné en plus-value quitteront .Jes entrepriises bretonnes pour conquérir le consommateur par leur niveau , de qualité contrôMe et gar~_ ntie. C'est le décollage que, tous ensemble, nous devons réussir.
– The solubility and the selectivity of αS1-, β- and κ-casein isolation from an industri-ally pro... more – The solubility and the selectivity of αS1-, β- and κ-casein isolation from an industri-ally produced spray-dried sodium caseinate as a function of temperature (20–40 ◦C) and of ethanol volume fraction (0–0.6) at pH 5.0–7.5 were studied. Reverse-phase high-performance liquid chro-matography and nitrogen balance were used as analytical methods. At 20 ◦C, casein solubility as a function of pH (5–7) and of the ethanol volume fraction (0–0.6) is quite well represented by an equation describing the ionisation process. αS1-, β- and κ-casein solubility and β- and κ-casein ex-traction selectivity decrease both with a rise in the ethanol volume fraction to 0.45–0.50 and with temperature increase. For αS1-casein, the selectivity increases to 67 % for an ethanol volume fraction of 0.47 at 29 ◦C. This fraction could be used as an emulsifying agent or for cream liqueur production or further purification.
Le domaine de l'invention est celui de la fermentation semi-continue d'une source carbone... more Le domaine de l'invention est celui de la fermentation semi-continue d'une source carbonee pour la fabrication d'un melange biologique a base d'acide propionique, selon un processus industrialisable. Le dispositif selon l'invention comprend avantageusement une cuve de fermentation (6) comportant deux boucles externes (9, 19) de circulation du substrat fermente: une boucle (9) de brassage du substrat fermente effectuant un prelevement et une reintroduction du substrat fermente dans la cuve (6) de fermentation, une boucle (19) d'extraction du melange (21) biologique a base d'acide propionique fabrique (21), incluant un module (22) de separation melange biologique fabrique (21)/bacteries (23). Le procede mis en ÷uvre est du type semi-continu, forme d'une succession de cycles de fermentation/extraction avec recyclage integral de la biomasse, puis recyclage partiel apres obtention d'une concentration en biomasse predeterminee.
FEMS Microbiology Reviews, 2020
Maternal health status is vital for the development of the offspring of humans, including physiol... more Maternal health status is vital for the development of the offspring of humans, including physiological health and psychological functions. The complex and diverse microbial ecosystem residing within humans contributes critically to these intergenerational impacts. Perinatal factors, including maternal nutrition, antibiotic use and maternal stress, alter the maternal gut microbiota during pregnancy, which can be transmitted to the offspring. In addition, gestational age at birth and mode of delivery are indicated frequently to modulate the acquisition and development of gut microbiota in early life. The early-life gut microbiota engages in a range of host biological processes, particularly immunity, cognitive neurodevelopment and metabolism. The perturbed early-life gut microbiota increases the risk for disease in early and later life, highlighting the importance of understanding relationships of perinatal factors with early-life microbial composition and functions. In this review, ...
Le Lait, 1993
HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific re... more HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.
Département de biochimie, de microbiologie et de bio-informatique, Faculté des sciences et de gén... more Département de biochimie, de microbiologie et de bio-informatique, Faculté des sciences et de génie, Groupe de recherche en écologie buccale, Faculté de médecine dentaire, Félix d’Hérelle Reference Center for Bacterial Viruses, Université Laval, Quebec City, Quebec, Canada, G1V 0A6. 2 Danisco USA Inc., 3329 Agriculture Drive, Madison, WI 53716, USA. 3 Danisco France SAS, Boîte Postale 10, F-86220 Dangé-Saint-Romain, France.
La presente invention concerne des procedes et des compositions permettant de moduler la resistan... more La presente invention concerne des procedes et des compositions permettant de moduler la resistance d'une cellule a un acide nucleique cible ou a un produit de la transcription de ce dernier. Dans des modes de realisation preferes, l'invention concerne des compositions et des procedes permettant d'utiliser un ou plusieurs genes ou proteines cas pour moduler la resistance d'une cellule a un acide nucleique cible ou a un produit de transcription de ce dernier. Dans des modes de realisation, la presente invention concerne des procedes et des compositions qui peuvent servir a mettre au point et a utiliser des combinaisons de souches et des rotations de cultures starter. Dans d'autres modes de realisation, la presente invention concerne des procedes de marquage et/ou d'identification de bacteries. Dans des modes de realisation preferes, la presente invention concerne des procedes consistant a utiliser des loci CRISPR pour determiner la virulence potentielle d'...