paulina bermejo - Academia.edu (original) (raw)
Papers by paulina bermejo
Phytotherapy Research, 2000
In the course of our search for plant natural products as antiviral agents, extracts of ten plant... more In the course of our search for plant natural products as antiviral agents, extracts of ten plants from the Iberian Peninsula were tested for antiviral activity against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and poliovirus type 1. Aqueous extracts of five of these medicinal plants, namely Nepeta nepetella (150-500 microg/mL), Nepeta coerulea (150-500 microg/mL), Nepeta tuberosa (150-500 microg/mL), Dittrichia viscosa (50-125 microg/mL) and Sanguisorba minor magnolii (50-125 microg/mL), showed a clear antiviral activity against two different DNA and RNA viruses, i.e. HSV-1 and VSV. Only the medicinal plant Dittrichia viscosa was active against an additional virus, poliovirus type 1.
Journal of Ethnopharmacology, 2006
Hexanic, dichloromethanic, ethanolic and aqueous extracts from Baccharis obtusifolia HBK, Bacchar... more Hexanic, dichloromethanic, ethanolic and aqueous extracts from Baccharis obtusifolia HBK, Baccharis latifolia (R. et P.) Pers., Baccharis pentlandii D.C. and Baccharis subulata Wedd., plants used in the traditional medicine of South America have been studied for their in vitro anti-inflammatory activity in cellular systems. Calcium ionophore A23187-stimulated mouse peritoneal macrophages were validated as a source of cyclooxygenase-1 (COX-1) (prostaglandin E 2 , PGE 2 ) and 5-lipoxygenase (5-LOX) (leukotriene C 4 , LTC 4 ), and mouse peritoneal macrophages stimulated with Escherichia coli lipopolysaccharide (LPS) were used for testing cyclooxygenase-2 (COX-2) (PGE 2 ), nitric oxide (NO) and tumour necrosis factor-␣ (TNF-␣) activity. Most of the extracts tested were active in all assays.
Phytotherapy Research, 1999
Folk medicinal plants are potential sources of useful therapeutic compounds including some with a... more Folk medicinal plants are potential sources of useful therapeutic compounds including some with antiviral activities. Extracts prepared from 10 South American medicinal plants (Baccharis trinervis, Baccharis teindalensis, Eupatorium articulatum, Eupatorium glutinosum, Tagetes pusilla, Neurolaena lobata, Conyza floribunda, Phytolacca bogotensis, Phytolacca rivinoides and Heisteria acuminata) were screened for in vitro antiviral activity against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and poliovirus type 1. The most potent inhibition was observed with an aqueous extract of B. trinervis, which inhibited HSV-1 replication by 100% at 50-200 mg/mL, without showing cytotoxic effects. Good activities were also found with the ethanol extract of H. acuminata and the aqueous extract of E. articulatum, which exhibited antiviral effects against both DNA and RNA viruses (HSV-1 and VSV, respectively) at 125-250 mg/mL. The aqueous extracts of T. pusilla (100-250 mg/mL), B. teindalensis (50-125 mg/mL) and E. glutinosum (50-125 mg/mL) also inhibited the replication of VSV, but none of the extracts tested had any effect on poliovirus replication.
Phytotherapy Research, 1997
Dichloromethane and ethanol extracts of 12 plants with a history of use in traditional medicine, ... more Dichloromethane and ethanol extracts of 12 plants with a history of use in traditional medicine, were tested for antiviral activity against herpes simplex type I. The most potent inhibition was shown by ethanol extracts of Eugenia jambos, Cistus populifolius, Lippia alba, Chiranthodendron pentadactylon and Tuberaria lignosa. These extracts, and others that had no effect, were chosen for more extensive studies against poliovirus type 1 and vesicular stomatitis virus. It was found that the ethanol extracts of Eugenia jambos, Chiranthodendron pentadactylon and Santolina oblongifolia inhibited the replication of VSV, but none of the extracts investigated had any effect on poliovirus replication.
Phytotherapy Research, 2000
In the course of our search for plant natural products as antiviral agents, extracts of ten plant... more In the course of our search for plant natural products as antiviral agents, extracts of ten plants from the Iberian Peninsula were tested for antiviral activity against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and poliovirus type 1. Aqueous extracts of five of these medicinal plants, namely Nepeta nepetella (150-500 microg/mL), Nepeta coerulea (150-500 microg/mL), Nepeta tuberosa (150-500 microg/mL), Dittrichia viscosa (50-125 microg/mL) and Sanguisorba minor magnolii (50-125 microg/mL), showed a clear antiviral activity against two different DNA and RNA viruses, i.e. HSV-1 and VSV. Only the medicinal plant Dittrichia viscosa was active against an additional virus, poliovirus type 1.
Journal of Natural Products, 1996
Four coumarins were isolated from the EtOAc extract of the flower-tops of Santolina oblongifolia ... more Four coumarins were isolated from the EtOAc extract of the flower-tops of Santolina oblongifolia Boiss. (Compositae). They were identified as 7-methoxycoumarin (herniarin) (1), 6,7-dihydroxycoumarin (aesculetin) (2), 6-methoxy-7-glucosidylcoumarin (scopolin) (3), and 6-hydroxy-7-methoxycoumarin (scopoletin) (4). This is the first report of the isolation of aesculetin and scopolin from the genus Santolina. The isolated coumarins showed marked activity as inhibitors of eicosanoid-release from ionophore-stimulated mouse peritoneal macrophages.
Journal of Ethnopharmacology, 2006
Hexanic, dichloromethanic, ethanolic and aqueous extracts from Baccharis obtusifolia HBK, Bacchar... more Hexanic, dichloromethanic, ethanolic and aqueous extracts from Baccharis obtusifolia HBK, Baccharis latifolia (R. et P.) Pers., Baccharis pentlandii D.C. and Baccharis subulata Wedd., plants used in the traditional medicine of South America have been studied for their in vitro anti-inflammatory activity in cellular systems. Calcium ionophore A23187-stimulated mouse peritoneal macrophages were validated as a source of cyclooxygenase-1 (COX-1) (prostaglandin E 2 , PGE 2 ) and 5-lipoxygenase (5-LOX) (leukotriene C 4 , LTC 4 ), and mouse peritoneal macrophages stimulated with Escherichia coli lipopolysaccharide (LPS) were used for testing cyclooxygenase-2 (COX-2) (PGE 2 ), nitric oxide (NO) and tumour necrosis factor-␣ (TNF-␣) activity. Most of the extracts tested were active in all assays.
Journal of Natural Products, 1997
The CH2Cl2 extract of Tanacetum microphyllum exhibited antiinflammatory activity on PMA-mouse ear... more The CH2Cl2 extract of Tanacetum microphyllum exhibited antiinflammatory activity on PMA-mouse ear model. Two antiinflammatory flavonoids, 5, 7-dihydroxy-3, 6, 4'-trimethoxyflavone (santin)(1) and 5, 7-dihydroxy-3, 4'-dimethoxyflavone (ermanin)(2), were isolated.
Phytotherapy Research, 2000
In the course of our search for plant natural products as antiviral agents, extracts of ten plant... more In the course of our search for plant natural products as antiviral agents, extracts of ten plants from the Iberian Peninsula were tested for antiviral activity against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and poliovirus type 1. Aqueous extracts of five of these medicinal plants, namely Nepeta nepetella (150-500 microg/mL), Nepeta coerulea (150-500 microg/mL), Nepeta tuberosa (150-500 microg/mL), Dittrichia viscosa (50-125 microg/mL) and Sanguisorba minor magnolii (50-125 microg/mL), showed a clear antiviral activity against two different DNA and RNA viruses, i.e. HSV-1 and VSV. Only the medicinal plant Dittrichia viscosa was active against an additional virus, poliovirus type 1.
Phytotherapy Research, 1999
Folk medicinal plants are potential sources of useful therapeutic compounds including some with a... more Folk medicinal plants are potential sources of useful therapeutic compounds including some with antiviral activities. Extracts prepared from 10 South American medicinal plants (Baccharis trinervis, Baccharis teindalensis, Eupatorium articulatum, Eupatorium glutinosum, Tagetes pusilla, Neurolaena lobata, Conyza floribunda, Phytolacca bogotensis, Phytolacca rivinoides and Heisteria acuminata) were screened for in vitro antiviral activity against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and poliovirus type 1. The most potent inhibition was observed with an aqueous extract of B. trinervis, which inhibited HSV-1 replication by 100% at 50-200 mg/mL, without showing cytotoxic effects. Good activities were also found with the ethanol extract of H. acuminata and the aqueous extract of E. articulatum, which exhibited antiviral effects against both DNA and RNA viruses (HSV-1 and VSV, respectively) at 125-250 mg/mL. The aqueous extracts of T. pusilla (100-250 mg/mL), B. teindalensis (50-125 mg/mL) and E. glutinosum (50-125 mg/mL) also inhibited the replication of VSV, but none of the extracts tested had any effect on poliovirus replication.
Journal of Ethnopharmacology, 1996
Six medicinal plant extracts from Venezuela, used in traditional medicine, were investigated for ... more Six medicinal plant extracts from Venezuela, used in traditional medicine, were investigated for their anti-inflammatory potential against adjuvant-carrageenan-induced inflammation (ACII). All doses expressed here are as equivalents of dried starting plant materials (1.50 g dry plant/kg body wt.). The most interesting plant extracts were Synedrella nod(flora, and the hexane leaf extract of Bursera simaruba. In ACII, orally administered extracts (at doses 40 and 80 mg/kg, respectively), inhibited both the acute and chronic phases of this experimental model of inflammation, mainly the chronic phase. These extracts exhibited potent anti-inflammatory activity daily throughout the experiment, and were as effective as reference drugs, phenylbutazone (80 mg/kg) and indomethacin (3 mg/kg), * Corresponding author. use of these drugs has a sound tradition, and their medicinal uses and general safety are well known to native peoples, their place has yet to be rationalized in therapeutics, using the current methodology. Scientific studies are therefore required to judge their efficacy and some of the medicinal properties popularly claimed, as well as other limitations to widen the scope of these drugs.
Journal of Ethnopharmacology, 2006
Hexanic, dichloromethanic, ethanolic and aqueous extracts from Baccharis obtusifolia HBK, Bacchar... more Hexanic, dichloromethanic, ethanolic and aqueous extracts from Baccharis obtusifolia HBK, Baccharis latifolia (R. et P.) Pers., Baccharis pentlandii D.C. and Baccharis subulata Wedd., plants used in the traditional medicine of South America have been studied for their in vitro anti-inflammatory activity in cellular systems. Calcium ionophore A23187-stimulated mouse peritoneal macrophages were validated as a source of cyclooxygenase-1 (COX-1) (prostaglandin E 2 , PGE 2 ) and 5-lipoxygenase (5-LOX) (leukotriene C 4 , LTC 4 ), and mouse peritoneal macrophages stimulated with Escherichia coli lipopolysaccharide (LPS) were used for testing cyclooxygenase-2 (COX-2) (PGE 2 ), nitric oxide (NO) and tumour necrosis factor-␣ (TNF-␣) activity. Most of the extracts tested were active in all assays.
Phytotherapy Research, 1997
Dichloromethane and ethanol extracts of 12 plants with a history of use in traditional medicine, ... more Dichloromethane and ethanol extracts of 12 plants with a history of use in traditional medicine, were tested for antiviral activity against herpes simplex type I. The most potent inhibition was shown by ethanol extracts of Eugenia jambos, Cistus populifolius, Lippia alba, Chiranthodendron pentadactylon and Tuberaria lignosa. These extracts, and others that had no effect, were chosen for more extensive studies against poliovirus type 1 and vesicular stomatitis virus. It was found that the ethanol extracts of Eugenia jambos, Chiranthodendron pentadactylon and Santolina oblongifolia inhibited the replication of VSV, but none of the extracts investigated had any effect on poliovirus replication.
Phytotherapy Research, 1999
Folk medicinal plants are potential sources of useful therapeutic compounds including some with a... more Folk medicinal plants are potential sources of useful therapeutic compounds including some with antiviral activities. Extracts prepared from 10 South American medicinal plants (Baccharis trinervis, Baccharis teindalensis, Eupatorium articulatum, Eupatorium glutinosum, Tagetes pusilla, Neurolaena lobata, Conyza floribunda, Phytolacca bogotensis, Phytolacca rivinoides and Heisteria acuminata) were screened for in vitro antiviral activity against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and poliovirus type 1. The most potent inhibition was observed with an aqueous extract of B. trinervis, which inhibited HSV-1 replication by 100% at 50-200 mg/mL, without showing cytotoxic effects. Good activities were also found with the ethanol extract of H. acuminata and the aqueous extract of E. articulatum, which exhibited antiviral effects against both DNA and RNA viruses (HSV-1 and VSV, respectively) at 125-250 mg/mL. The aqueous extracts of T. pusilla (100-250 mg/mL), B. teindalensis (50-125 mg/mL) and E. glutinosum (50-125 mg/mL) also inhibited the replication of VSV, but none of the extracts tested had any effect on poliovirus replication.
Journal of Ethnopharmacology, 1998
The present study was carried out to investigate the anti-inflammatory and antioxidant and proper... more The present study was carried out to investigate the anti-inflammatory and antioxidant and properties of ethanol extract of Crinum asiaticum (Syn: Crinum amabile) leaf. The carrageenan induced paw edema model was used to evaluate the anti-inflammatory activity in Wister albino rats. In this study, Crinum asiaticum leaf extract was administered to animals at the dose of 2gm/kg and 1gm/kg body weight and the obtained effects were compared with commercially available anti-inflammatory drug diclofenac sodium (10 mg/kg) for carrageenan induced paw edema test. Antioxidant activity of the Crinum asiaticum ethanol leaf extract was assessed by DPPH free radical scavenging method. Crinum asiaticum leaf extract showed significant effect in the reduction of the paw edema induced by carrageenan at the dose of 2gm/kg and 1gm/kg body weight where 40.8% and 8.76% of paw edema were reduced at the 4 th hour of administration of extract. These results indicate that Crinum asiaticum leaf extract have potent anti-inflammatory effect. Leaf extract also showed DPPH free radical scavenging effect compared with ascorbic acid. IC 50 value of ascorbic acid and leaf extract was found 13.26µg/ml and 71.4µg/ml respectively.
Phytotherapy Research, 2000
In the course of our search for plant natural products as antiviral agents, extracts of ten plant... more In the course of our search for plant natural products as antiviral agents, extracts of ten plants from the Iberian Peninsula were tested for antiviral activity against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and poliovirus type 1. Aqueous extracts of five of these medicinal plants, namely Nepeta nepetella (150-500 microg/mL), Nepeta coerulea (150-500 microg/mL), Nepeta tuberosa (150-500 microg/mL), Dittrichia viscosa (50-125 microg/mL) and Sanguisorba minor magnolii (50-125 microg/mL), showed a clear antiviral activity against two different DNA and RNA viruses, i.e. HSV-1 and VSV. Only the medicinal plant Dittrichia viscosa was active against an additional virus, poliovirus type 1.
Phytotherapy Research, 1997
Dichloromethane and ethanol extracts of 12 plants with a history of use in traditional medicine, ... more Dichloromethane and ethanol extracts of 12 plants with a history of use in traditional medicine, were tested for antiviral activity against herpes simplex type I. The most potent inhibition was shown by ethanol extracts of Eugenia jambos, Cistus populifolius, Lippia alba, Chiranthodendron pentadactylon and Tuberaria lignosa. These extracts, and others that had no effect, were chosen for more extensive studies against poliovirus type 1 and vesicular stomatitis virus. It was found that the ethanol extracts of Eugenia jambos, Chiranthodendron pentadactylon and Santolina oblongifolia inhibited the replication of VSV, but none of the extracts investigated had any effect on poliovirus replication.
Journal of Natural Products, 1996
Four coumarins were isolated from the EtOAc extract of the flower-tops of Santolina oblongifolia ... more Four coumarins were isolated from the EtOAc extract of the flower-tops of Santolina oblongifolia Boiss. (Compositae). They were identified as 7-methoxycoumarin (herniarin) (1), 6,7-dihydroxycoumarin (aesculetin) (2), 6-methoxy-7-glucosidylcoumarin (scopolin) (3), and 6-hydroxy-7-methoxycoumarin (scopoletin) (4). This is the first report of the isolation of aesculetin and scopolin from the genus Santolina. The isolated coumarins showed marked activity as inhibitors of eicosanoid-release from ionophore-stimulated mouse peritoneal macrophages.
Journal of Ethnopharmacology, 2006
Hexanic, dichloromethanic, ethanolic and aqueous extracts from Baccharis obtusifolia HBK, Bacchar... more Hexanic, dichloromethanic, ethanolic and aqueous extracts from Baccharis obtusifolia HBK, Baccharis latifolia (R. et P.) Pers., Baccharis pentlandii D.C. and Baccharis subulata Wedd., plants used in the traditional medicine of South America have been studied for their in vitro anti-inflammatory activity in cellular systems. Calcium ionophore A23187-stimulated mouse peritoneal macrophages were validated as a source of cyclooxygenase-1 (COX-1) (prostaglandin E 2 , PGE 2 ) and 5-lipoxygenase (5-LOX) (leukotriene C 4 , LTC 4 ), and mouse peritoneal macrophages stimulated with Escherichia coli lipopolysaccharide (LPS) were used for testing cyclooxygenase-2 (COX-2) (PGE 2 ), nitric oxide (NO) and tumour necrosis factor-␣ (TNF-␣) activity. Most of the extracts tested were active in all assays.
Journal of Natural Products, 1996
Four coumarins were isolated from the EtOAc extract of the flower-tops of Santolina oblongifolia ... more Four coumarins were isolated from the EtOAc extract of the flower-tops of Santolina oblongifolia Boiss. (Compositae). They were identified as 7-methoxycoumarin (herniarin) (1), 6,7-dihydroxycoumarin (aesculetin) (2), 6-methoxy-7-glucosidylcoumarin (scopolin) (3), and 6-hydroxy-7-methoxycoumarin (scopoletin) (4). This is the first report of the isolation of aesculetin and scopolin from the genus Santolina. The isolated coumarins showed marked activity as inhibitors of eicosanoid-release from ionophore-stimulated mouse peritoneal macrophages.
Phytotherapy Research, 2000
In the course of our search for plant natural products as antiviral agents, extracts of ten plant... more In the course of our search for plant natural products as antiviral agents, extracts of ten plants from the Iberian Peninsula were tested for antiviral activity against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and poliovirus type 1. Aqueous extracts of five of these medicinal plants, namely Nepeta nepetella (150-500 microg/mL), Nepeta coerulea (150-500 microg/mL), Nepeta tuberosa (150-500 microg/mL), Dittrichia viscosa (50-125 microg/mL) and Sanguisorba minor magnolii (50-125 microg/mL), showed a clear antiviral activity against two different DNA and RNA viruses, i.e. HSV-1 and VSV. Only the medicinal plant Dittrichia viscosa was active against an additional virus, poliovirus type 1.
Journal of Ethnopharmacology, 2006
Hexanic, dichloromethanic, ethanolic and aqueous extracts from Baccharis obtusifolia HBK, Bacchar... more Hexanic, dichloromethanic, ethanolic and aqueous extracts from Baccharis obtusifolia HBK, Baccharis latifolia (R. et P.) Pers., Baccharis pentlandii D.C. and Baccharis subulata Wedd., plants used in the traditional medicine of South America have been studied for their in vitro anti-inflammatory activity in cellular systems. Calcium ionophore A23187-stimulated mouse peritoneal macrophages were validated as a source of cyclooxygenase-1 (COX-1) (prostaglandin E 2 , PGE 2 ) and 5-lipoxygenase (5-LOX) (leukotriene C 4 , LTC 4 ), and mouse peritoneal macrophages stimulated with Escherichia coli lipopolysaccharide (LPS) were used for testing cyclooxygenase-2 (COX-2) (PGE 2 ), nitric oxide (NO) and tumour necrosis factor-␣ (TNF-␣) activity. Most of the extracts tested were active in all assays.
Phytotherapy Research, 1999
Folk medicinal plants are potential sources of useful therapeutic compounds including some with a... more Folk medicinal plants are potential sources of useful therapeutic compounds including some with antiviral activities. Extracts prepared from 10 South American medicinal plants (Baccharis trinervis, Baccharis teindalensis, Eupatorium articulatum, Eupatorium glutinosum, Tagetes pusilla, Neurolaena lobata, Conyza floribunda, Phytolacca bogotensis, Phytolacca rivinoides and Heisteria acuminata) were screened for in vitro antiviral activity against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and poliovirus type 1. The most potent inhibition was observed with an aqueous extract of B. trinervis, which inhibited HSV-1 replication by 100% at 50-200 mg/mL, without showing cytotoxic effects. Good activities were also found with the ethanol extract of H. acuminata and the aqueous extract of E. articulatum, which exhibited antiviral effects against both DNA and RNA viruses (HSV-1 and VSV, respectively) at 125-250 mg/mL. The aqueous extracts of T. pusilla (100-250 mg/mL), B. teindalensis (50-125 mg/mL) and E. glutinosum (50-125 mg/mL) also inhibited the replication of VSV, but none of the extracts tested had any effect on poliovirus replication.
Phytotherapy Research, 1997
Dichloromethane and ethanol extracts of 12 plants with a history of use in traditional medicine, ... more Dichloromethane and ethanol extracts of 12 plants with a history of use in traditional medicine, were tested for antiviral activity against herpes simplex type I. The most potent inhibition was shown by ethanol extracts of Eugenia jambos, Cistus populifolius, Lippia alba, Chiranthodendron pentadactylon and Tuberaria lignosa. These extracts, and others that had no effect, were chosen for more extensive studies against poliovirus type 1 and vesicular stomatitis virus. It was found that the ethanol extracts of Eugenia jambos, Chiranthodendron pentadactylon and Santolina oblongifolia inhibited the replication of VSV, but none of the extracts investigated had any effect on poliovirus replication.
Phytotherapy Research, 2000
In the course of our search for plant natural products as antiviral agents, extracts of ten plant... more In the course of our search for plant natural products as antiviral agents, extracts of ten plants from the Iberian Peninsula were tested for antiviral activity against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and poliovirus type 1. Aqueous extracts of five of these medicinal plants, namely Nepeta nepetella (150-500 microg/mL), Nepeta coerulea (150-500 microg/mL), Nepeta tuberosa (150-500 microg/mL), Dittrichia viscosa (50-125 microg/mL) and Sanguisorba minor magnolii (50-125 microg/mL), showed a clear antiviral activity against two different DNA and RNA viruses, i.e. HSV-1 and VSV. Only the medicinal plant Dittrichia viscosa was active against an additional virus, poliovirus type 1.
Journal of Natural Products, 1996
Four coumarins were isolated from the EtOAc extract of the flower-tops of Santolina oblongifolia ... more Four coumarins were isolated from the EtOAc extract of the flower-tops of Santolina oblongifolia Boiss. (Compositae). They were identified as 7-methoxycoumarin (herniarin) (1), 6,7-dihydroxycoumarin (aesculetin) (2), 6-methoxy-7-glucosidylcoumarin (scopolin) (3), and 6-hydroxy-7-methoxycoumarin (scopoletin) (4). This is the first report of the isolation of aesculetin and scopolin from the genus Santolina. The isolated coumarins showed marked activity as inhibitors of eicosanoid-release from ionophore-stimulated mouse peritoneal macrophages.
Journal of Ethnopharmacology, 2006
Hexanic, dichloromethanic, ethanolic and aqueous extracts from Baccharis obtusifolia HBK, Bacchar... more Hexanic, dichloromethanic, ethanolic and aqueous extracts from Baccharis obtusifolia HBK, Baccharis latifolia (R. et P.) Pers., Baccharis pentlandii D.C. and Baccharis subulata Wedd., plants used in the traditional medicine of South America have been studied for their in vitro anti-inflammatory activity in cellular systems. Calcium ionophore A23187-stimulated mouse peritoneal macrophages were validated as a source of cyclooxygenase-1 (COX-1) (prostaglandin E 2 , PGE 2 ) and 5-lipoxygenase (5-LOX) (leukotriene C 4 , LTC 4 ), and mouse peritoneal macrophages stimulated with Escherichia coli lipopolysaccharide (LPS) were used for testing cyclooxygenase-2 (COX-2) (PGE 2 ), nitric oxide (NO) and tumour necrosis factor-␣ (TNF-␣) activity. Most of the extracts tested were active in all assays.
Journal of Natural Products, 1997
The CH2Cl2 extract of Tanacetum microphyllum exhibited antiinflammatory activity on PMA-mouse ear... more The CH2Cl2 extract of Tanacetum microphyllum exhibited antiinflammatory activity on PMA-mouse ear model. Two antiinflammatory flavonoids, 5, 7-dihydroxy-3, 6, 4'-trimethoxyflavone (santin)(1) and 5, 7-dihydroxy-3, 4'-dimethoxyflavone (ermanin)(2), were isolated.
Phytotherapy Research, 2000
In the course of our search for plant natural products as antiviral agents, extracts of ten plant... more In the course of our search for plant natural products as antiviral agents, extracts of ten plants from the Iberian Peninsula were tested for antiviral activity against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and poliovirus type 1. Aqueous extracts of five of these medicinal plants, namely Nepeta nepetella (150-500 microg/mL), Nepeta coerulea (150-500 microg/mL), Nepeta tuberosa (150-500 microg/mL), Dittrichia viscosa (50-125 microg/mL) and Sanguisorba minor magnolii (50-125 microg/mL), showed a clear antiviral activity against two different DNA and RNA viruses, i.e. HSV-1 and VSV. Only the medicinal plant Dittrichia viscosa was active against an additional virus, poliovirus type 1.
Phytotherapy Research, 1999
Folk medicinal plants are potential sources of useful therapeutic compounds including some with a... more Folk medicinal plants are potential sources of useful therapeutic compounds including some with antiviral activities. Extracts prepared from 10 South American medicinal plants (Baccharis trinervis, Baccharis teindalensis, Eupatorium articulatum, Eupatorium glutinosum, Tagetes pusilla, Neurolaena lobata, Conyza floribunda, Phytolacca bogotensis, Phytolacca rivinoides and Heisteria acuminata) were screened for in vitro antiviral activity against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and poliovirus type 1. The most potent inhibition was observed with an aqueous extract of B. trinervis, which inhibited HSV-1 replication by 100% at 50-200 mg/mL, without showing cytotoxic effects. Good activities were also found with the ethanol extract of H. acuminata and the aqueous extract of E. articulatum, which exhibited antiviral effects against both DNA and RNA viruses (HSV-1 and VSV, respectively) at 125-250 mg/mL. The aqueous extracts of T. pusilla (100-250 mg/mL), B. teindalensis (50-125 mg/mL) and E. glutinosum (50-125 mg/mL) also inhibited the replication of VSV, but none of the extracts tested had any effect on poliovirus replication.
Journal of Ethnopharmacology, 1996
Six medicinal plant extracts from Venezuela, used in traditional medicine, were investigated for ... more Six medicinal plant extracts from Venezuela, used in traditional medicine, were investigated for their anti-inflammatory potential against adjuvant-carrageenan-induced inflammation (ACII). All doses expressed here are as equivalents of dried starting plant materials (1.50 g dry plant/kg body wt.). The most interesting plant extracts were Synedrella nod(flora, and the hexane leaf extract of Bursera simaruba. In ACII, orally administered extracts (at doses 40 and 80 mg/kg, respectively), inhibited both the acute and chronic phases of this experimental model of inflammation, mainly the chronic phase. These extracts exhibited potent anti-inflammatory activity daily throughout the experiment, and were as effective as reference drugs, phenylbutazone (80 mg/kg) and indomethacin (3 mg/kg), * Corresponding author. use of these drugs has a sound tradition, and their medicinal uses and general safety are well known to native peoples, their place has yet to be rationalized in therapeutics, using the current methodology. Scientific studies are therefore required to judge their efficacy and some of the medicinal properties popularly claimed, as well as other limitations to widen the scope of these drugs.
Journal of Ethnopharmacology, 2006
Hexanic, dichloromethanic, ethanolic and aqueous extracts from Baccharis obtusifolia HBK, Bacchar... more Hexanic, dichloromethanic, ethanolic and aqueous extracts from Baccharis obtusifolia HBK, Baccharis latifolia (R. et P.) Pers., Baccharis pentlandii D.C. and Baccharis subulata Wedd., plants used in the traditional medicine of South America have been studied for their in vitro anti-inflammatory activity in cellular systems. Calcium ionophore A23187-stimulated mouse peritoneal macrophages were validated as a source of cyclooxygenase-1 (COX-1) (prostaglandin E 2 , PGE 2 ) and 5-lipoxygenase (5-LOX) (leukotriene C 4 , LTC 4 ), and mouse peritoneal macrophages stimulated with Escherichia coli lipopolysaccharide (LPS) were used for testing cyclooxygenase-2 (COX-2) (PGE 2 ), nitric oxide (NO) and tumour necrosis factor-␣ (TNF-␣) activity. Most of the extracts tested were active in all assays.
Phytotherapy Research, 1997
Dichloromethane and ethanol extracts of 12 plants with a history of use in traditional medicine, ... more Dichloromethane and ethanol extracts of 12 plants with a history of use in traditional medicine, were tested for antiviral activity against herpes simplex type I. The most potent inhibition was shown by ethanol extracts of Eugenia jambos, Cistus populifolius, Lippia alba, Chiranthodendron pentadactylon and Tuberaria lignosa. These extracts, and others that had no effect, were chosen for more extensive studies against poliovirus type 1 and vesicular stomatitis virus. It was found that the ethanol extracts of Eugenia jambos, Chiranthodendron pentadactylon and Santolina oblongifolia inhibited the replication of VSV, but none of the extracts investigated had any effect on poliovirus replication.
Phytotherapy Research, 1999
Folk medicinal plants are potential sources of useful therapeutic compounds including some with a... more Folk medicinal plants are potential sources of useful therapeutic compounds including some with antiviral activities. Extracts prepared from 10 South American medicinal plants (Baccharis trinervis, Baccharis teindalensis, Eupatorium articulatum, Eupatorium glutinosum, Tagetes pusilla, Neurolaena lobata, Conyza floribunda, Phytolacca bogotensis, Phytolacca rivinoides and Heisteria acuminata) were screened for in vitro antiviral activity against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and poliovirus type 1. The most potent inhibition was observed with an aqueous extract of B. trinervis, which inhibited HSV-1 replication by 100% at 50-200 mg/mL, without showing cytotoxic effects. Good activities were also found with the ethanol extract of H. acuminata and the aqueous extract of E. articulatum, which exhibited antiviral effects against both DNA and RNA viruses (HSV-1 and VSV, respectively) at 125-250 mg/mL. The aqueous extracts of T. pusilla (100-250 mg/mL), B. teindalensis (50-125 mg/mL) and E. glutinosum (50-125 mg/mL) also inhibited the replication of VSV, but none of the extracts tested had any effect on poliovirus replication.
Journal of Ethnopharmacology, 1998
The present study was carried out to investigate the anti-inflammatory and antioxidant and proper... more The present study was carried out to investigate the anti-inflammatory and antioxidant and properties of ethanol extract of Crinum asiaticum (Syn: Crinum amabile) leaf. The carrageenan induced paw edema model was used to evaluate the anti-inflammatory activity in Wister albino rats. In this study, Crinum asiaticum leaf extract was administered to animals at the dose of 2gm/kg and 1gm/kg body weight and the obtained effects were compared with commercially available anti-inflammatory drug diclofenac sodium (10 mg/kg) for carrageenan induced paw edema test. Antioxidant activity of the Crinum asiaticum ethanol leaf extract was assessed by DPPH free radical scavenging method. Crinum asiaticum leaf extract showed significant effect in the reduction of the paw edema induced by carrageenan at the dose of 2gm/kg and 1gm/kg body weight where 40.8% and 8.76% of paw edema were reduced at the 4 th hour of administration of extract. These results indicate that Crinum asiaticum leaf extract have potent anti-inflammatory effect. Leaf extract also showed DPPH free radical scavenging effect compared with ascorbic acid. IC 50 value of ascorbic acid and leaf extract was found 13.26µg/ml and 71.4µg/ml respectively.
Phytotherapy Research, 2000
In the course of our search for plant natural products as antiviral agents, extracts of ten plant... more In the course of our search for plant natural products as antiviral agents, extracts of ten plants from the Iberian Peninsula were tested for antiviral activity against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and poliovirus type 1. Aqueous extracts of five of these medicinal plants, namely Nepeta nepetella (150-500 microg/mL), Nepeta coerulea (150-500 microg/mL), Nepeta tuberosa (150-500 microg/mL), Dittrichia viscosa (50-125 microg/mL) and Sanguisorba minor magnolii (50-125 microg/mL), showed a clear antiviral activity against two different DNA and RNA viruses, i.e. HSV-1 and VSV. Only the medicinal plant Dittrichia viscosa was active against an additional virus, poliovirus type 1.
Phytotherapy Research, 1997
Dichloromethane and ethanol extracts of 12 plants with a history of use in traditional medicine, ... more Dichloromethane and ethanol extracts of 12 plants with a history of use in traditional medicine, were tested for antiviral activity against herpes simplex type I. The most potent inhibition was shown by ethanol extracts of Eugenia jambos, Cistus populifolius, Lippia alba, Chiranthodendron pentadactylon and Tuberaria lignosa. These extracts, and others that had no effect, were chosen for more extensive studies against poliovirus type 1 and vesicular stomatitis virus. It was found that the ethanol extracts of Eugenia jambos, Chiranthodendron pentadactylon and Santolina oblongifolia inhibited the replication of VSV, but none of the extracts investigated had any effect on poliovirus replication.
Journal of Natural Products, 1996
Four coumarins were isolated from the EtOAc extract of the flower-tops of Santolina oblongifolia ... more Four coumarins were isolated from the EtOAc extract of the flower-tops of Santolina oblongifolia Boiss. (Compositae). They were identified as 7-methoxycoumarin (herniarin) (1), 6,7-dihydroxycoumarin (aesculetin) (2), 6-methoxy-7-glucosidylcoumarin (scopolin) (3), and 6-hydroxy-7-methoxycoumarin (scopoletin) (4). This is the first report of the isolation of aesculetin and scopolin from the genus Santolina. The isolated coumarins showed marked activity as inhibitors of eicosanoid-release from ionophore-stimulated mouse peritoneal macrophages.
Journal of Ethnopharmacology, 2006
Hexanic, dichloromethanic, ethanolic and aqueous extracts from Baccharis obtusifolia HBK, Bacchar... more Hexanic, dichloromethanic, ethanolic and aqueous extracts from Baccharis obtusifolia HBK, Baccharis latifolia (R. et P.) Pers., Baccharis pentlandii D.C. and Baccharis subulata Wedd., plants used in the traditional medicine of South America have been studied for their in vitro anti-inflammatory activity in cellular systems. Calcium ionophore A23187-stimulated mouse peritoneal macrophages were validated as a source of cyclooxygenase-1 (COX-1) (prostaglandin E 2 , PGE 2 ) and 5-lipoxygenase (5-LOX) (leukotriene C 4 , LTC 4 ), and mouse peritoneal macrophages stimulated with Escherichia coli lipopolysaccharide (LPS) were used for testing cyclooxygenase-2 (COX-2) (PGE 2 ), nitric oxide (NO) and tumour necrosis factor-␣ (TNF-␣) activity. Most of the extracts tested were active in all assays.
Journal of Natural Products, 1996
Four coumarins were isolated from the EtOAc extract of the flower-tops of Santolina oblongifolia ... more Four coumarins were isolated from the EtOAc extract of the flower-tops of Santolina oblongifolia Boiss. (Compositae). They were identified as 7-methoxycoumarin (herniarin) (1), 6,7-dihydroxycoumarin (aesculetin) (2), 6-methoxy-7-glucosidylcoumarin (scopolin) (3), and 6-hydroxy-7-methoxycoumarin (scopoletin) (4). This is the first report of the isolation of aesculetin and scopolin from the genus Santolina. The isolated coumarins showed marked activity as inhibitors of eicosanoid-release from ionophore-stimulated mouse peritoneal macrophages.