priya sekar - Academia.edu (original) (raw)

Papers by priya sekar

The collagenase comprise a class of enzymes which are designed to cleave the triple helix of nati... more The collagenase comprise a class of enzymes which are designed to cleave the triple helix of native collagen. The cell free extract of Pila globosa was fractionate with sephadex G-75 column chromatography, SDS PAGE. From the tissue the collagenase enzymes was identified and purified by using assay methods. The collagenolytic activity were also measured thus the collagenolytic enzymes have profound implication in an assay methods. The collagenolytic activity were also measured thus the collagenolytic enzyme have profound implication in an assay of cellular fraction. More over the hepatopancreas the land snail collagenase was isolated and characterized food source of proteolytic enzymes with high specific activities.

Leucas aspera is a perennial herb with woody root stock hairs erect and spreading. In the present... more Leucas aspera is a perennial herb with woody root stock hairs erect and spreading. In the present study the leaves and root extracts of Leucas aspera were investigated for invitro antibacterial activity and phytochemical analysis. The antibacterial activity of Leucas aspera was studied using double diffusion method and the activity was tested against the human pathogens like Bacillus cereus,Klebisella pneumoniae,Staphylococcus aureus,Pseudomonas aeruginosa and Enterobacter faecalis. On the basis of zone of inhibition the results have been illustrated. It is suggested that the leaves and roots of this plant contain good antibacterial activity that could be developed and used as a natural antibacterial agent.

Totally ten Actinomycetes species were isolated from marine soil of Vedharanyam, Nagappattinam di... more Totally ten Actinomycetes species were isolated from marine soil of Vedharanyam, Nagappattinam district, East Coast of India, Viz, Actinomadura hibisca, Actinopolyspora halophila, Amycolata autotrophila, Rhodococcus, coprophilus, Saccharopolyspora hirsute, S.spinosa, S.albus, Streptoverticilium album and S.salmonis. Among these species isolated, S albus and S cyaneus have been selected for enzyme studies. These organisms could be vital sources for the discovery of industrially useful enzymes. Fermentation of cellulase and lipase were carried out and S. cyaneus showed the cellulase and lipase activity of 0.028 IU/ml and 0.617 IU/ml. The cellulase and lipase activity produced by S. albus was 0.021 IU/ml and 0.513 IU/ml respectively.

The present research was to isolate and identify cellulolytic bacteria from the gut region of the... more The present research was to isolate and identify cellulolytic bacteria from the gut region of the local termites Odontotermes obesus. The isolates were cultures in a medium containing carboxy methyl-cellulose and cellobiose. The bacterial species were tentatively identified by using the Bergey’s manual. The species were all novel strains and identified as Bacillus sp.,Pseudomonas and Cellulomonas sp. respectively.

The present study demonstrated that Amaranthus spinosus leaves have antioxidant compound which wa... more The present study demonstrated that Amaranthus spinosus leaves have antioxidant compound which was purified using aqueous methanol extract by chromatographical technique silica gel G-60 column chromatography and by TLC. The biological antioxidant compound were screened by ¹H-NMR and ¹³C-NMR and its activity were measured by ABTS assay, inhibition of lipid peroxidation, superoxide radical and hydroxyl radical scavenging assay.

Vibrios are the most common genera associated with crustaceans. The genus Vibrio were isolated fr... more Vibrios are the most common genera associated with crustaceans. The genus Vibrio were isolated from marine prawn (Penaeus monodon). The isolates were screened for vibriocin production. The best producer was identified as Vibrio parahaemolyticus .The maximum production of Vibrio spp. was manifested at 37ºC at pH 9, after 20h of incubation. The Vibrio spp. was confirmed by the presence of biochemical tests methyl red, VP and oxidase test. Its activity was enhanced in the presence of TCBS medium and withstands autoclaving temperature and showed activity even after prolonged chloroform treatment.

In present study, the methanol method for carotenoids extraction from the intestine waste of Gall... more In present study, the methanol method for carotenoids extraction from the intestine waste of Gallus gallus (chicken) was introduced. The antimicrobial activity of the carotenoids extract against Escherichia coli, Aspergillus niger, Aspergillus flavus, Saccharomyce scerevisiae and Bacillus subtilis was also elucidated. Results showed that the optimum extraction conditions was at a temperature of 50°C, a solvent-solid ratio of 10:1 and duration of 30 min.The disc diffusion method and minimum inhibitory concentration(MIC) determination showed that the extract has a wide spectrum of antimicrobial activity against E.coli, B.subtilis and S.cerevisiae with their inhibition zones ranging from 8.90mm to 15.45mm and the MIC ranging from 16.75μg/ml to 90μg/ml.However,it showed no inhibition effect on A.niger and A.flavus.

Calotropis procera is a shrub or small tree, rarely branched, woody and fissured. In the present ... more Calotropis procera is a shrub or small tree, rarely branched, woody and fissured. In the present study, the leaves and latex extracts of C.procera were investigated for invitro antibacterial activity and phytochemical analysis. The antibacterial activity of C.procera was studied using disc diffusion method and the activity was tested against the human pathogens like E.coli, Pseudomonas aeruginosa, Staphylococus aureus, Enterobacter feacelis. On the basis of zone of inhibition the results have been illustrated. It is suggested that the leaves and latex of this plant contain good antibacterial activity that could be developed and used as natural antibacterial agents.

Airborne fungal spores constitute one of the important components of microbial population of ambi... more Airborne fungal spores constitute one of the important components of microbial population of ambient air. Aeromycological study was conducted for two years (January, 2012 to December, 2013) using Rotorod air sampler in the indoor air of certain residential dwellings in Thanjavur District, Tamil Nadu, India. During the present investigation, 15 fungal types were detected of which Trichoderma spores contributed highest concentration. Maximum concentration of fungal spores was observed in the month of August in 2012 and November in 2013 while minimum concentration was observed in the month of September in both years. Allergenically significant fungal spores responsible for serious health hazards were detected throughout the investigation period. Seasonal variation of airborne fungal spores was correlated with meteorological parameters.

Fatty acid (UFA) biosynthesis is essential for the maintenance of membrane structure and function... more Fatty acid (UFA) biosynthesis is essential for the maintenance of membrane structure and function in many groups of anaerobic bacteria. In this study isolation of cellular fatty acid distributed in unknown rhizobial strains collected from five different soil samples by pour plate method using YEMA medium. The five soil sample are namely Klt 1, Alg 2,Bdl 3, Rf 4 and Mdk 5.The isolated rhizobial sample was identified based on morphological and biochemical characterization. Then the purified culture was mass multiplied and used for isolation of fatty acid. The fatty acid profile of five different rhizobial isolates were identified by GC and the result of fatty acid was totally seven fatty acids from Rhizobial isolates. Among them the Myrestic acid (C:14) were present all the isolates respectively.

Volatile oils are one of the groups of secondary metabolites produced by some plants. Their proba... more Volatile oils are one of the groups of secondary metabolites produced by some plants. Their probable users against burn microbes have received little attention. Burn swabs obtained from ten burned patients at Government hospital, led to the isolation of bacteria using standard methods, the microorganisms were isolated. Then chloroform, methanol and aqueous extract of volatile of Ixora Cocciniea against S.aureus, E.coli, Pseudomonas aeruginosa, P.vulgaris and K.pnemoniae exhibited antibacterial were found efficacious respectively.

Objective: To evaluate the antipyretic effect of the ethanolic extract of leaf and bark of Diospy... more Objective: To evaluate the antipyretic effect of the ethanolic extract of leaf and bark of Diospyros virginiana Linn against brewer yeast induced pyrexia model in albino rats of both sexes was investigated.
Methods: The Pyrexia was induced by subcutaneously injecting 20% w/v brewer's yeast suspension (10 ml/kg) into the animals. After the injection, the rectal temperature of each rat was measured. Paracetamol (150 mg/kg) was used as the standard drug.
Results: The results showed that the ethanol extracts of leaf of Diospyros virginiana possessed significant antipyretic effect compared to the bark extract.
Conclusion: This study provides evidences for the antipyretic activity of Diospyros virginiana which could partly contribute to its ethno medical use.

Chlorella vulgaris is widely cultivated micro algae found in water bodies as it is rich in nutrie... more Chlorella vulgaris is widely cultivated micro algae found in water bodies as it is rich in nutrients and its component
of phytoplankton. C.vulgaris is useful in biomass production in commercial level as it contains proteins and
minerals. In this study the value added biochemical compounds in C.vulgaris were analyzed from different cultures.
The amount of total carotenoids, protein, vitamin, pigments and phenolic compounds were examined. C.vulgaris
extracts possessed great potential antibacterial activities against four bacterial strains with inhibition zones ranged
8-15mm and MICs values ranges from 25-40ɥg/mL.Thus, it could be suggested that the C.vulgaris is useful biosystem
for production of biochemical compounds possess an antimicrobial principles.

The present study of phytochemical analysis in the leaves and bark powder extract with absolute e... more The present study of phytochemical analysis in the leaves and bark powder extract with absolute ethanol, the
phytochemical compound screened by GC-MS method. In this GC-MS analysis, 40 bioactive phytochemical
compounds and 30 compounds were identified in leaves and bark of Diospyros virginiana. The identified compounds
predominantly phenolic derivatives are present included Hydrocarbons, Carbohydrates, Fatty Acid, Fatty Acid ester,
Alcoholic compounds, Alkaloids, Ketones and Alkenes compounds. These different active phytochemicals have been
found to possess a wide range of activities, which may help in the protection against incurable diseases.

Antibacterial activity of ethanolic extracts of plant Cryptomeria japonica used for the treatment... more Antibacterial activity of ethanolic extracts of plant Cryptomeria japonica used for the treatment of parasitic
diseases were examined with four bacteria (two Gram positive-Bacillus subtilis and Staphylococcus aureus and
two Gram negative - Escherichia coli and Salmonella typhi) following disc diffusion method. Plant showed
antibacterial property with B. subtilis, 16 (48 %) plants with S. aureus, 18 (54 %) plants with E. coli and 8 (24 %)
with S.typhi and were found inhibitory against all the bacteria. Plants utilized to combat enteric and urinary
tract infections showed antibacterial activity preferably to Gram-negative organisms. Careful standardization
for these medicinal plants must be instituted for their development as safe, efficacious, antibacterial of plant
origin conservation of traditional knowledge.

This experiment was carried out to study the effect of organic manure(Farmyard manure and poultry... more This experiment was carried out to study the effect of organic manure(Farmyard manure and
poultry manure at a rate of 75m3/ha) and biofertilizers (Rhizobium and azospirillum at a rate of 980 gm/ha)
as their interactions on growth and yield using drip irrigation system.the results indicated that the
appilication of a poultry manure as an organic fertilization to recorded the maximum values.Eclipta alba
treated with rhizobium biofertilizer gave the highest value, while plants treated with azospirillum
respectively.

The ethanolic extract of the leaves and bark of Diospyros virginiana was screened for antidiarrhe... more The ethanolic extract of the leaves and bark of Diospyros virginiana was screened for antidiarrheal effects. The extract was evaluated for castor oil- induced diarrhea and intestinal transit in rats. Diospyros virginiana significantly dose-dependently reduced frequency of stooling in castor oil-induced diarrhea and intestinal motility in rats. Ethanolic leaves extracts reduces diarrheal burden than ethanolic bark extract. These findings suggest that the ethanolic extract of the leaves and bark of Diospyros virginiana may contain some biologically active ingredients that are active for the treatment of diarrhea in herbal traditional medicine

An indigenious herbal tree Diospyros virginiana was evaluated for hypoglycaemic activity on adult... more An indigenious herbal tree Diospyros virginiana was evaluated for hypoglycaemic activity on adult swiss albino rats each using normo glycemic, glucose loaded and streptozotocin induced hypoglycemic rats. Glibenclamide (3.0mg/kg) was used as reference standard for activity comparision. The formulation showed promising results that is comparable to that of the reference standard glibenclamide. The present work justifies the use of the herbal formulation in the traditional practice for the treatment of diabetes.